• 제목/요약/키워드: Male sex

검색결과 2,847건 처리시간 0.033초

웅성 특이적 SRY 및 ZFY 유전자를 이용한 쇠고기 성(性) 판별 (Sex Identification of Bovine Meat Using Male Specific SRY and ZFY Genes)

  • 신성철;정구용;정의룡
    • 한국축산식품학회지
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    • 제27권3호
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    • pp.351-356
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    • 2007
  • 본 연구는 포유류의 Y-염색체상에 존재하는 SRY 및 ZFY의 웅성 특이적 성 결정 유전자를 이용하는 PCR 기법으로 쇠고기 성판별 기술을 개발하고자 수행하였다. 성 결정 유전자 영역의 특정 염기서열을 포함하는 primer를 각각 설계 합성하고 이들 primer를 이용하여 PCR증폭을 실시한 다음, 각각의 증폭산물을 1.5% agarose gel에 전기영동하여 웅성 특이적 DNA band의 증폭여부를 확인하였다. SRY 유전자에서 웅성개체 쇠고기는 1,348 bp 크기의 단편을 가진 DNA band가 검출되었으나, 자성개체의 경우 DNA band가 전혀 검출되지 않은 것을 확인 할 수 있었다. 또한, ZFY 유전자에서 웅성개체의 쇠고기는 979 bp 크기의 단편을 가진 DNA band가 모두 검출되었으나, 자성개체의 쇠고기에서는 역시 DNA band가 전혀 검출되지 않았다. 즉, SRY 및 ZFY 유전자는 모두 숫소에서 유래한 쇠고기에서 웅성 특이적인 DNA band가 정확히 검출된 반면 암소에서 유래한 쇠고기에서는 웅성 특이적 DNA band가 전혀 검출되지 않았다. 또한 쇠고기 성 감별법을 도축 후 가공 유통판매단계에 실제 활용 가능성을 검증하고자 시중에 유통되고 있는 등심 및 갈비 포장육 350시료를 무작위 추출하여 성 판별을 실시한 결과 숫소가 252시료(72%) 그리고 암소가 98두(28%)로 조사되었다. 따라서 본 연구에서 개발한 SRY 또는 ZFY의 웅성 특이적 성 결정 유전자를 이용하는 쇠고기 성 감별기술은 생산단계는 물론 도축 후 가공 유통 판매되고 있는 모든 쇠고기의 암수 성감별을 위한 유용한 DNA 표지인자로 활용할 수 있을 것으로 기대된다.

가야시대 인골의 치아에 의한 성별 및 연령추정 (Sex and Age Determination of the Kaya s Ancient Human Skeletal Remains via Dental Approaches)

  • Mee-Eun Kim;Myung-Yun Ko;Bong-Soo Park
    • Journal of Oral Medicine and Pain
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    • 제21권2호
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    • pp.225-242
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    • 1996
  • The author studied to determine the sex and age of 8 ancient human skeletal remains, which had excavated from ancient tombs located in Yeanri, Kimhae. Some kinds of personal identification methods their skulls and teeth were used for this study and the results were obtained as follows : 1. Sex determination was possible in ancient teeth from 4th to 5th century, using detection of X-Y homologous amelogenin gene by polymerase chain reaction. 2. DNA analysis proved that the materials examined were all male, but which always did not coincide with the results from other methods for sex determination including comparison of sexual differentiation of cranium and teeth and use of discriminant functions in the dental measurement. 3. There was little difference of the estimated ages between the methods by regression of pulp cavity and attrition in teeth. The ages from these two methods always did not coincide with ones from evaluation closure of cranial and palatal sutures. 4. Sex and age of the materials were determined as follows ; $\cdot$ Y9 was estimated to be male in early sixties. $\cdot$ Yl2 was estimated to be male in late twenties to early thirties. $\cdot$ Y37 was estimated to be male in early forties. $\cdot$ Y70 was estimated to be male in early sixties. $\cdot$ Y87 was estimated to be male in late forties. $\cdot$ Y109 was estimated to be male in early forties. $\cdot$ Yl29 was estimated to be male in late thirties to early forties. $\cdot$ Yl42 was estimated to be male in late fifties to early sixties.

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독신의 심리적 성향에 대한 연구 (A Study of the Psychological Characteristics of a Single Life)

  • 장현숙
    • 가정과삶의질연구
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    • 제20권6호
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    • pp.185-195
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    • 2002
  • This study examined the psychological aspects of a single life with particular focus on the personality & emotional characteristics, the social & behavioral characteristics, marriage, happiness, and sex of singles. The sample consisted of 160 single male and female aged 30 to 49. The results of the questionnaire survey showed that the singles tended to be self-centered, especially when they did not have a plan to get married, and that most of the singles in the 30s wanted to have a friend of the other sex. In the view of marriage, young, male singles were more likely to form a family than old, female singles. Also, female singles, bereaved or divorced, were shown to have fear about marriage and negative attitudes toward marriage more than male, never-married singles did. Regarding the extent of happiness, old, female (rather than young, male) singles tended to be more satisfied with a single life. Besides, old, religious singles were less likely to feel lonely than young, unreligious singles. In addition, it appeared that college-graduated singles had rather liberal view on sex although singles generally showed rather conservative toward sex in this study. Therefore, the findings reveal that singles were self-centered, social, positive view on marriage, somewhat happy of a single life, and having a conservative attitude about sex. Further understanding of a single life in modem society is needed in future studies.

Dehydroepiandrosterone(DHEA)의 투여에 의한 rat 흉대동맥의 반응성 변화 (Responsiveness of the Thoracic Aorta in Rats Treated with Dehydroepiandrosterone (DHEA))

  • 박관하
    • Biomolecules & Therapeutics
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    • 제9권2호
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    • pp.119-124
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    • 2001
  • In order to determine the role of dehydroepiandrosterone (DHEA), the important sex-steroid hormone precursor, in vascular reactivity in rats, animals were treated for two weeks with DHEA or sex hormones, and the vascorelaxant and contractile responses of isolated aorta were examined. DHEA diminished the acetylcholine (ACh)-induced relaxation in female rats, while the drug was without effect in males. Testoterone lowered the vasorelaxant activity to ACh in either sex. 17$\beta$-Estradiol enhanced ACh-induced vasorelaxation in male rats, but this female sex hormone did not influence in females. In male rats, the androgen receptor antagonist flutamide also enhanced vasorelaxant action of ACh. When the male rat aorta was incubated in vitro with a nitric oxide (NO) synthase inhibitor L-NAME, phenylephrine-induced contraction was greatly potentiated in DHEA-pretreated rats compared to control ones. The present results suggest that DHEA stimulates mainly androgen in female, but both androgen and estrogen in male rats. The participation of NO In the modulation of vascular reactivity with pretreated DHEA was also considered.

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중학생의 인터넷음란물 접속과 성 의식의 관계 (Relationship between Access to Lewd Internet Contents by Middle School Students and Their Awareness of Sex)

  • 임종인;최인숙
    • 한국학교ㆍ지역보건교육학회지
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    • 제4권
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    • pp.117-139
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    • 2003
  • The purpose of this research lies in presenting logical viability for the measures that curtail access to lewd Internet contents by middle school students amidst the reality in which lewd contents are circulated freely through the Internet, a medium that the middle school students find most easy to access. In order to establish right form of awareness towards sex, this research identified the ways they access the lewd Internet contents, their reaction after the exposure to those contents, their knowledge of sex, their concerns regarding sex and their accessibility to sexual activities in order to conduct a comparative analysis on the relationship between lewd Internet contents and their awareness of sex. First, realities of accessing lewd Internet contents and reactions according to the demographics of middle school students There isa significant difference in the experience of accessing lewd Internet contents in terms of gender. Mostly, male students tend to access the contents more. As for the way they access the lewd Internet contents, both male and female students replied that they access through spam mail of lewd nature. Thus, measures to address this problem are needed urgently. As to when they first accessed the lewd contents, most of the research subjects replied that they accessed either in elementary school period or in the early middle school period. This shows that most of the students got exposed to lewd contents even before they could establish positive, correct awareness of sex. Thus, there is a risk that they may formulate wrong kind of sexual awareness. Accordingly, it is necessary to develop measures through focused sex education. Students are divided into two groups according to the time they spend on the contents averagely: those who spend over one hour and those who spend less than an hour on the lewd contents. If the students spending longer hours are not to be checked and properly guided, it may lead to increasing cases of sexual delinquencies due to their wrongly formed awareness of sex. When the question of existence(non-existence) of guardian was addressed, students with both parents tend to access the lewd Internet contents in a more diverse manner and tend to access more compared to those students from single parent or no-parent families. Accordingly, guardians need to pay attention to how their children are using the Internet. Second, awareness of sex depending on the middle school students' demographics In case of sexual knowledge, middle school students shows relatively high level of knowledge. In particular, female students are found more knowledgeable than male students, and the students in upper years are more knowledgeable as well. As a result, this research recommends that the students in lower years should be guided with more basc and detailed information, while those in upper years need to be taught to form and express their own thoughts and attitudes and to build up independence on this matter. In case of worries about sex, both male and female students don't worry too much about it. However, male students are more concerned about sex than female students in a more diverse ways. As for the differences by academic year, concerns for sex increase, as students get older. Accordingly, sex education that helps establish sound perception of the opposite sex and that focuses on the etiquettes that one must adhere to at the presence of the opposite sex need to be conducted against middle school students. In case of accessibility to sex, male students manifest higher tendency than female students. As for the differences by academic year, those in the first and second years show higher accessibility than those in their third year. In general, younger students tend to be more open-minded toward sex. Accordingly, students in lower academic years need to undergo basic knowledge oriented sex education, whereas those in upper academic years need to undergo discussion centered sex education where they subject to questions regarding their attitude and opinion. Third, relationship between the reactions after the exposure to the lewd Internet contents and their awareness of sex As the frequency of contact with lewd Internet contents increases, awareness of sex increases as well. Thus, the lewd Internet contents contribute to the increase in students' concern for sex as well as increase in accessibility to sex, which eventually become barriers to students' establishment of a healthy perception of sex. Reactions to sex after the exposure to lewd Internet contents and awareness of sex indicate a significant correlation. However, negative correlation is manifested with knowledge of sex. Thus, it is possible to know that the greater access to lewd Internet contents does not necessarily translate into increase in knowledge of sex. However, the study showed there is a correlation between concerns for sex and the level of accessibility to sex. In more detail, the more reactions to the contents they show, the more concerns for sex they have and the more positively they take acceptability to sex. Moreover, it is necessary to develop necessary measures since textbooks today do not include measures needed to address the lewd Internet contents. Given the above findings, it is necessary to continue to complement structural measures in order to prevent easy access of lewd Internet contents by middle school students. Moreover, it is necessary to be considerate of the students so that they themselves can form a healthy Internet culture and grow up within positive framework for the sex education.

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대학생과 고등학생의 성역할 정체감과 성 고정관념에 대한 비교조사 (Sex-Role Identity & Stereotypes of Students in High School and College)

  • 박영숙;김영임;박연환
    • 한국학교보건학회지
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    • 제11권1호
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    • pp.51-62
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    • 1998
  • The purposes of this study are to identify sex-role Identity and stereotypes in students-specifically, to compare men with women, and high school students with college students The subjects consisted of 283 college students and 392 high school students in Seoul A Bem's Sex-Role Inventory translated by Hur, Sookja for sex-role identity stereotype scales and modified by Kim, Dongil was used The major findings were as follows 1 Androgyny type was the most prevalent of all four types (35 3%) in college male students, but undifferentiated type was the most common (36 8%) for college female students 2 The sex-role identity distribution of high school male and female students was undifferentiated, androgyny, feminity, and masculinity in that order 3 There was a significant difference between male and female students in the perception sex stereotypes Women were inclined to oppose traditional sex-roles rather than men 4 There were significant differences between high school and college students in the perception of domestic sex-roles, appearance and occupational characteristics, as well as the psychosocial traits of sex stereotypes The college students tended to resist traditional sex-roles rather than high school students 5 There were no significant differences between sex-role types and sex stereotypes In conclusion, women have difficulty in developing a sex-role identity owing to the contradiction between a woman's desirable sex-role and her feminity sex-role identity.

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A Cultural Comparison of Sex Role Identity and Attitude toward Grooming and Recreational Apparel Shopping Behavior among Male Consumers

  • Lee, Jaeil;Lee, Yoon-Jung
    • 한국의류산업학회지
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    • 제15권4호
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    • pp.565-573
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    • 2013
  • This study focused on the cultural differences between South Korea and the U.S. in terms of male consumers' sex role attitude and its influence on grooming and apparel shopping behavior. Purposive samples of American and South Korean males aged between 20 and 40 years were surveyed. The sample sizes were 219 and 233 for American and South Korean consumers, respectively. The data were analyzed by structural equation modeling and ANOVA using SPSS 12.0 and AMOS 14.0. The results indicated that only grooming was influenced by the perceived femininity in the case of South Korean men; however, the model for American men indicated a significant positive influence of femininity on grooming and recreational apparel shopping behavior. In other words, American male consumers who perceive themselves feminine were more likely to be engaged in grooming and recreational apparel shopping behavior. On the other hand, for South Korean men, recreational apparel shopping behavior was not influenced by their sex role attitude, or whether they considered themselves feminine or masculine. This means that recreational apparel shopping behavior is a gender-specific behavior in the U.S., but not in South Korea. The findings of this study indicated that culture has influence on consumers' approach to shopping and appearance. South Korean male consumers were more likely to acknowledge themselves as being feminine, enjoy apparel shopping and grooming compared to American male consumers.

배양세포의 성염색질에 관한 세포학적 연구 1. 배양기간을 달리한 고양이, 몰못트, 닭의 배양세포에 있어서의 성염색질에 대하여

  • 강영선;박상대
    • 한국동물학회지
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    • 제8권2호
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    • pp.37-41
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    • 1965
  • The frequency and pattern of sex chromatin in primary cultured cells of kidney cortex of cats and guinea-pigs, and muscle of chick embroys were examined and compared to those of in vivo condition, with special reference to the various cultured stages. 1. In cat, the frequencies of sex chromatin positive I of peripheral position were average 62.7% in female, and 15% in male, whereas those of non-peripherla position were 5.8% in female and 0.1% in male. The incident proportion between them showed a marked difference-approximately 10 times higher in female than male. These results failry indicated that a distinct nuclear dimorphism with regard to the sex chromatin positive I was established in cultured cells. The position of sex chormatin was usually peripheral location. The tendency of frequencies , with reference to the cultured stages, was low count in primary extracted and initial stage cells , but it showed a peaked frequency in 10-13 days after primary culture, and after that the frequencies were decreased gradually. Compared between I vitro and in vivo condition of the same tissues, the cells in vivo exhibited the sex chromatin in high frequency at the peak showed stage. 2. In guinea-pig , the frequencyies of peripheral positive I were 36.8% in female and 6.3% in male, while non-peripheral positions were 6.1% in female and 3.5% inmale. Its incident was a rate of nearly 4 times higher in female than male. The nuclear dimorphism was also established in cultrued cells of guinea-pig. The position and the incident frquency showed a similar pattern as in cat except the primary extracted cells. 3. In chick embryo, the frequencies of sex chromatin positive I of peripheral position were 38.2% in female, and 18.3% in male, non-peripheral position, however, was hardly to find. These results suggest that the definite sexual dimorphism was unable to find in chick embryo cultured cells. The position and the incident tendency were a similar pattern as in above mammals and the frequency was higher in vitro cells.

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북방전복 (Haliotis discus hannai) 의 성별에 따른 성장형질 및 유전모수 추정 (Estimation of Genetic Parameter and Growth Traits by Sex of Pacific Abalone, Haliotis discus hannai)

  • 박철지;박종원;김보라;정규현;김영진;손윤석;김경길
    • 한국패류학회지
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    • 제32권4호
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    • pp.249-254
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    • 2016
  • 북방전복을 대상으로 성별에 따른 성장형질의 변이를 이용한 선발육종의 가능성 여부를 확인하고자, 실제로 암수 1:1 수정을 통하여 10가계를 생산 관리하여 성별에 따른 성장형질의 표현형 측정과 유전모수를 추정하였다. 그 결과 성비율은 1:1로 일반적 전복류의 성비와 같은 결과를 나타내었으며, 성별에 따른 성장은 모든 성장형질에 있어 암컷이 수컷보다 유의적으로 큰 것으로 나타났다 (p < 0.05). 이러한 결과는 선발을 할 때 성별에 따른 성장차이를 이용하여 양식전복의 성장률 향상을 할 수 있는 가능성을 시사하고 있다.

Y 염색체 특이성 DNA분리와 단일 H-Y 항체 개발에 의한 토끼의 수정란 성 감별에 관한 연구 II. PCR을 이용한 Y 염색체 특이성 DNA의 증폭에 의한 토끼 수정란의 성 감별 (Studies on Isolaton of Y-specific DNA Marker and Development of Monoclonal H-Y Antibody for Embryo Sexing in Rabbit II. Sex Determination of Rabbit Embryo by PCR Amplified Y-specific DNA)

  • 박영일;임경순;한재용;남경우;황규춘;박화춘
    • 한국가축번식학회지
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    • 제20권2호
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    • pp.89-99
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    • 1996
  • The purpose of this study was to develop the diagnosis techniques for sex determination of rabbit embryos at preimplantation stage. To detect male specific sequences using polymerase chain reaction, two genes functional on sex determination including SRY and ZFX/Y genes were targeted using multiple oligonucleotide primer sets. Three of them for conserved SRY gene were used for appropriate amplification pattern, and then only one primer set #3 proved to be most efficient, showing male-specific strong signal ofamplified sequences. Using this male specific bandsfrom human, cattle, pig and mouse, the gender of rabbit was determined. As an another system for sex determination system, amplified 910bp fragment from ZFX/Y was digested with several restriction endonuclease and showed gender specific restriction fragments only by Hinf I. Using two different system for sex identification of rabbit in this study, blind tests for 17 samples was conducted and showed identical results from two different methods. And then, amplification limit of PCR reaction for template DNA was estimated using various amounts of DNA for both SRY and ZFX/Y systems, resulted as 20pg and 800pg, respectively. With this results, test for gender identification of rabbit embryos were performed using SRY derived amplification system. From total 22 embryos selected for its developmental state 18 were identified as male embryos, showing significant difference from expected sex ratio 1:1. This biased sex ratio was interpreted as to have been caused by the fact, reported by the fact, reported by several researchers, that male embryos develop more rapidly and are more resistant against the in vitro manipulation than female embryos.

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