• Clinical and Experimental Reproductive Medicine
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• v.50 no.3
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• pp.143-153
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• 2023

Objective: Men's sexual health plays an important role in male fertility and childbearing, as it is associated with factors such as sexual desire, healthy spermatogenesis, and erectile function. In various cultures, medicinal plants have been utilized to address male sexual issues, including infertility and erectile dysfunction. Despite recent advancements in medical science for treating male impotence, some men opt for herbal supplements as an alternative, given that numerous herbs have the potential to enhance male sexual performance. The Apiaceae family is one of the oldest plant families used for medicinal purposes. Ferula, a genus within this family, comprises approximately 170 different species worldwide. Members of this genus possess numerous therapeutic properties due to the presence of various compounds. This article aims to explore the potential impacts of Ferula plants on the male reproductive system. Methods: This review article was prepared by searching for terms including Ferula and "aphrodisiac," Ferula and "spermatogenesis," and Ferula and "male reproductive system." Relevant information was gathered through electronic databases, including ISI Web of Knowledge, PubMed, and Google Scholar. Results: The findings indicated that relatively comprehensive studies have been conducted in this area, revealing that certain Ferula species have been employed in folk medicine to boost fertility and libido. Recent research has corroborated these effects. Conclusion: It is hoped that new aphrodisiac compounds with fewer side effects can be isolated from Ferula plants in the future.

• Korean Journal of Plant Tissue Culture
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• v.25 no.5
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• pp.309-313
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• 1998

RAPD (Random Amplified Polymorphic DNA) analysis was conducted to Schisandra nigra plants in order to select the specific markers for monoecious and dioecious individuals. RAPD results using eighty random 10-mer primers revealed that S. nigra had a different banding pattern from S. chinensis and Kadsura japonica. When DNA isolated from leaves of monoecious and dioecious plants were used as PCR template, only five primers, OPA-17, OPA-19, OPB-03, OPB-09 and OFB-16, showed polymorphic band patterns. No variation in banding profiles within male or female individuals was observed when these five primers were used whereas three monoecious plants (No 1, No 2 and No 3) showed different banding patterns one another, A 750 bp segment was amplified by primer OPB-3 from male individuals. On the other hand, two segments, 950 bp and 1690 bp, with OPA-19 and 700 bp of segment with OPB-3 were amplified in female individuals. These result indicate that the specific buds of male and female S. nigra could be used as genetic markers for the early discrimination of male and female individuals.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.3
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• pp.281-288
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• 2007

Photoperiod sensitive genetic male sterile (PGMS) rice is sterile mutant controlled by photoperiod. A PGMS mutant 920S was sterile grown under long-day (LD) photoperiod (14 h light/10 h dark) but fertile grown under short-day (SD) photoperiod (10 h light/14 h dark). Proteome analysis revealed that 12 protein spots were differentially expressed in the spikelets of 920S plants either treated with LD or SD photoperiod. Among these proteins, three proteins including chlorophyll a/b binding protein, vacuolar ATPase ${\beta}-subunit,\;{\alpha}-tubulin$ and an unknown protein were more than three-fold abundant in the spikelet of the SD-treated plants than those of the LD-treated plants. On the other hand, eight proteins including acetyl transferase, 2, 3- biphosphoglycerate, aminopeptidase N, pyruvate decarboxylase, 60S acidic ribosomal protein and three unknown protein spots were more abundant in the spikelets of the LD-treated plants than those of the SD-treated plants. The results suggest that the observed proteins may be involved in sterile or fertile pollen development under LD or SD photoperiod respectively in the PGMS mutant rice.

• Journal of Radiation Industry
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• v.4 no.3
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• pp.239-246
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• 2010

The aim of this study is selection of the male-sterile plant for inhibiting transgene flow through gamma-irradiation ($^{60}Co$) at the pollination and fertilization cycle of herbicide-tolerant genetically modified (GM) zoysiagrass (Zoysia japonica Steud.). High frequencies of plant mutations were obtained about 18% from $M_1$ generation at the doses (10 to 50 Gy). We also found that some $M_1$ plants showed male-sterile plants using de-husked seeds and comparison of stainable pollen using $KI-I_2$ solution. Besides the effects of irradiation on pollination and fertilization cycle, various other mutations like dwarf, cold tolerance, increasing grains and mass were observed. Four of dwarfism plants were selected through comparison of morphological characteristic between control and mutants during 4 years. These results demonstrated that the gamma-irradiation on pollination and fertilization cycle is very effective to induce the various mutations, and the male-sterile mutants are useful for controlling transgene flow and developing of high quality turfgasses.

• Journal of Plant Biology
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• v.26 no.3
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• pp.141-150
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• 1983

Antithamnion sparsum Tokida isolated from the southern and western coasts of Korea was investigated in culture, comparing the morphological character with A. defectum Kylin from the Pacific North America. A. sparsum basically showed a Polysiphonia-type life history. However, it sometimes exhibited a monoecious reproducton and the carpospores released from the cystocarp by self-fertilization unexpectedly developed into the plants bearing spermatangia alone. These male plants were not functional up to 60 days culture. The results of intraspecific crosses between populations of A. sparsum were positive and the hybrid carpospores gave rise to normal tetrasporophytes. On the other hand, theinterspecific crosses between A. sparsum and A. defectum were successful partly, evidenced by the gonimoblast development and the release of carpospores in case of A. sparsum (male)$\times$A. defectum (female), but not in case of A. sparsum (female)$\times$A. defectum (male). Thus, the both species were still under the speciation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.3
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• pp.99-107
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• 1965

Thirteen varieties of hemp, Cannabinus sativa L., consisting of four Italian, one Japanese and eight local varieties were used in the study on the fiber content and a satisfactory selection for the higher-fiber-yielding plants. In addition, the relationship among fiber contents at the ball setting stage, and at the maturity stage, primary fiber content, and total fiber content were determined. The results of the study are summarized as follow: 1. Fiber weight of the matured male plant expressed as percentage of the fiber weight to dried stems varied from 12.09 to 26.71 percent for the Italian varieties, 14.25% for the Japanese variety and ranged from 6.59 to 16.79 for the local varieties. It was shown that on the average, Italian varieties contained more fiber compared with the other varieties. 2. On the same basis, the average percent age of fiber for Italian male plants was 20.77 while it was 20.96 for female plants. For the Japanese variety, similar figures were 14.24 for male plants and 15.43 for female plants. By comparison, the average percent fiber for the male plants of Korean varieties was only 10.34 but averaged 16.79 for female plants. In this experiment, female plants contained a greater percentage of fiber than male plants. 3. Statistically significant correlations were obtained between fiber content at the ball setting stage and fiber content of the mature plant. The correlation coefficient was r=0.942＊＊, while the regression equation was Y=0.28+1.03X. These data indicate that plant. selection by early testing at the ball setting stage is an effective method for the improvement of fiber content. 4. The correlation between primary fiber content and total fiber content was r=0.913＊＊, with a regression equation of Y=12.01+1.20X. It should thus be possible to obtain plants of higher total fiber content at maturity by the selection of plants which have a higher content of primary fiber at the ball setting stage. 5. From a study of the transverse sections from the hemp plant it was shown that Italian varieties have wide bands of primary and secondary fibers with narrow areas of woody material, while both Korean local varieties and Japanese varieties have narrow bands of primary and secondary fiber and wide areas of woody material. For hemp improvement, it is certain that a variety which has a narrow area of woody substances and wide area of fiber in its stem should be selected.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.214-219
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• 2009

The current study was based on a survey of 429 middle school students (202 male and 227 female students) who lived in Ansan City. Most students (74%) had an experience in horticultural activities during or after elementary school years (male 43%, female 41%). Middle school students answered that they most frequently learned about growing up vegetables and plants among horticultural activities (male 73%, female 79%) and they thought horticultural activities are very important for education purpose such as environmental education. The most preferred horticultural activities program was utilizing vegetables and plants of which students generally had no experiences. That male students (46%) answered that the most interested aspect of horticultural activities in growth process of vegetables and plants while female answered (36%) the importance of plant life. However the most unfavorite aspect of horticultural activities was the time-consuming as a 58%, and then boring (33%).

• Journal of Life Science
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• v.21 no.12
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• pp.1659-1665
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• 2011

In Citrus, an $F_1$ segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu ${\times}$ C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the $F_1$ population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 ($x^2$ =2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding $F_1$ progenies in Citrus.

• Molecules and Cells
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• v.20 no.3
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• pp.416-422
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• 2005

We previously used Southern blot analysis to detect restriction-length polymorphisms between male fertile and cytoplasmic male sterile (CMS) cytoplasms at the coxII and atp6 loci of the mtDNA of Capsicum annuum L. Two copies of atp6 were found in each male fertile and CMS pepper lines. Interestingly, one of the copies of atp6 in CMS pepper was a 3'-truncated pseudogene. The open reading frame of the coxII gene was the same in the fertile (N-) and CMS (S-) lines. However, the nucleotide sequence in the S-cytoplasm diverged from that in the N-cytoplasm 41 bp downstream of the stop codon. To develop CMS-specific sequence-characterized amplified region (SCAR) markers, inverse PCR was performed to characterize the nucleotide sequences of the 5' and 3' flanking regions of mitochondrial atp6 and coxII from the cytoplasms of male fertile (N-) and CMS (S-) pepper plants. Based on these data, two CMS-specific SCAR markers, 607 and 708 bp long, were developed to distinguish N-cytoplasm from S-cytoplasm by PCR. The CMS-specific PCR bands were verified for 20 cultivars containing either N- or S-cytoplasm. PCR amplification of CMS-specific mitochondrial nucleotide sequences will allow quick and reliable identification of the cytoplasmic types of individual plants at the seedling stage, and assessment of the purity of $F_1$ seed lots. The strategy used in this report for identifying CMS-specific markers could be adopted for many other crops where CMS is used for F1 seed production.

• Plant Biotechnology Reports
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• v.4 no.1
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• pp.95-99
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• 2010

Decamer RAPD primers were tested on dioeceious and hermaphrodite plants of Commiphora wightii to identify sex-specific molecular markers. Sixty different random decamer primers were screened out of which only three primers were found to be associated with sex expression. A ~1,280-bp fragment from the primer OPN06 was found to be present in all the female individuals. Another primer OPN 16 produced a unique ~400-bp amplification product in only hermaphrodite individuals. The third marker, OPA20 amplified a ~1,140-bp fragment from female and hermaphrodite DNAs, but failed to do so from the male plant DNAs.