• Biomolecules & Therapeutics
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• v.31 no.3
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• pp.340-349
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• 2023

Mad2B (Mad2L2), the human homolog of the yeast Rev7 protein, is a regulatory subunit of DNA polymerase ζ that shares sequence similarity with the mitotic checkpoint protein Mad2A. Previous studies on Mad2B have concluded that it is a mitotic checkpoint protein that functions by inhibiting the anaphase-promoting complex/cyclosome (APC/C). Here, we demonstrate that Mad2B is activated in response to cisplatin-induced DNA damage. Mad2B co-localizes at nuclear foci with DNA damage markers, such as proliferating cell nuclear antigen and gamma histone H2AX (γ-H2AX), following cisplatin-induced DNA damage. However, unlike Mad2A, the binding of Mad2B to Cdc20 does not inhibit the activity of APC/C in vitro. In contrast to Mad2A, Mad2B does not localize to kinetochores or binds to Cdc20 in spindle assembly checkpoint-activated cells. Loss of the Mad2B protein leads to damaged nuclei following cisplatin-induced DNA damage. Mad2B/Rev7 depletion causes the accumulation of damaged nuclei, thereby accelerating apoptosis in human cancer cells in response to cisplatin-induced DNA damage. Therefore, our results suggest that Mad2B may be a critical modulator of DNA damage response.

• The Korean Journal of Physiology and Pharmacology
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• v.27 no.5
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• pp.427-436
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• 2023

Mitotic arrest deficient 2 like 2 (Mad2L2, also known as Mad2B), the human homologue of the yeast Rev7 protein, is a regulatory subunit of DNA polymerase ζ that shares high sequence homology with Mad2, the mitotic checkpoint protein. Previously, we demonstrated the involvement of Mad2B in the cisplatin-induced DNA damage response. In this study, we extend our findings to show that Mad2B is recruited to sites of DNA damage in human cancer cells in response to cisplatin treatment. We found that in undamaged cells, Mad2B exists in a complex with Polζ-Rev1 and the APC/C subunit Cdc27. Following cisplatin-induced DNA damage, we observed an increase in the recruitment of Mad2B and Cdc20 (the activators of the APC/C), to the complex. The involvement of Mad2B-Cdc20-APC/C during DNA damage has not been reported before and suggests that the APC/C is activated following cisplatin-induced DNA damage. Using an in vitro ubiquitination assay, our data confirmed Mad2B-dependent activation of APC/C in cisplatin-treated cells. Mad2B may act as an accelerator for APC/C activation during DNA damage response. Our data strongly suggest a role for Mad2B-APC/C-Cdc20 in the ubiquitination of proteins involved in the DNA damage response.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.8B
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• pp.1506-1517
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• 1999

In this paper, we propose two fast block matching algorithm using the distribution of mean absolute difference (MAD) at the search region overlapped with neighbor blocks and pixel subsapmling. The proposed methods use the lower and upper bound of MAD at the overlapped search region which is calculated from the MAD of neighbor block at that search position and MAD between the current block and neighbor block. In the first algorithm, we can reduce the computational complexity by executing the block matching operation at the only necessary search points. That points are selected using the lower bound of MAD. In the second algorithm, we use the statictical distribution of actual MAD which exists between the lower bound and upper bound of MAD. By using the statistical distribution of actual MAD, we can significantly reduce the computational complexity for motion estimation. after striking space key 2 times.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.05a
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• pp.83-86
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• 2005

The aim of this study is to establish the indoor noise limits of apartment houses for mad traffic noise. To achieve this goal, psycho-acoustic experiments were carried out with the mad traffic sound sources modulated by the transmission loss characteristics of the external windows. As a result of this study, followings are suggested. 1) On correlation between dose level and psycho-acoustical response, the initial level of negative feeling is located on $40.1{\sim}40.6$ Leq dB(A). 2) On the degree of satisfaction to road traffic noise, near 35% Point being same dissatisfaction degree is to be assumed $40{\sim}41$ dB(A) of indoor noise level presented into three vocabulary. It is suggested to be reasonable level of 40 dB(A) on the indoor noise limits for intruding road traffic noise, and it is appropriate to be the 5dB level difference between grades.

• Journal of Life Science
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• v.16 no.5
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• pp.828-833
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• 2006

Maize (Zea mays L.) pistil cell death and stamen cell arrest are pivotal process on the sex determination, which diverges from bisexual state of floral meristem to unisexual state in staminate or pistillate floret. We investigated the temporal and spatial distribution of cell cycle gene expression during maize sex determination. The positive regulatory genes of cell cycle, cyclin A, cyclin B, cyclin dependent kinase (CDK) and Mad2 were highly expressed in the developing pistil and stamen but the expression was disappeared in the dying pistil and arresting stamens. In contrast, the negative regulatory genes of cell cycle, Wee1 and CDK inhibitor (CKI) were expressed in the arresting stamens in the wild-type ear and tasselseed2 mutant tassel, however, these genes were not detected in dying pistil although the cyclin B gene expression was disappeared. These results suggest that both the pistil cell death and stamen cell arrest process in maize sex determination are involved in cell cycle regulation, but the different expression patterns of negative regulatory cell cycle genes in the arresting stamens and aborting pistils suggest that the two processes may have distinctive modes of action.

• Journal of The Korean Astronomical Society
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• v.33 no.1
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• pp.47-55
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• 2000

Nonpotential characteristics of magnetic fields in AR 5747 are examined using Mees Solar Observatory magnetograms taken on Oct. 20, 1989 to Oct. 22, 1989. The active region showed such violent flaring activities during the observational span that strong X-ray flares took place including a 2B/X3 flare. The magnetogram data were obtained by the Haleakala Stokes Polarimeter which provides simultaneous Stokes profiles of the Fe I doublet 6301.5 and 6302.5. A nonlinear least square method was adopted to derive the magnetic field vectors from the observed Stokes profiles and a multi-step ambiguity solution method was employed to resolve the $180^{\circ}$ ambiguity. From the ambiguity-resolved vector magnetograms, we have derived a set of physical quantities characterizing the field configuration, which are magnetic flux, vertical current density, magnetic shear angle, angular shear, magnetic free energy density, a measure of magnetic field discontinuity MAD and linear force-free coefficient. Our results show that (1) magnetic nonpotentiality is concentrated near the inversion line in the flaring sites, (2) all the physical parameters decreased with time, which may imply that the active region was in a relaxation stage of its evolution, (3) 2-D MAD has similar patterns with other nonpotential parameters, demonstrating that it can be utilized as an useful parameter of flare producing active region, and (4) the linear force-free coefficient could be a evolutionary indicator with a merit as a global nonpotential parameter.

• Journal of Korean Society of Forest Science
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• v.108 no.4
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• pp.592-599
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• 2019

This study aimed to develop an equation for estimating stem volume for Larix kaempferiin South Korea using independent variables, diameter at breast height (DBH), and height as being closely associated with stem volume. Analysis was conducted on the growth performance of 2,840 Larix kaempferi samples across South Korea after felling them and gleaning diameter data according to both stem height and stem analyses. In order to test the fitness of six different stem taper equations, empirical assessment was conducted for fitness index (FI), bias, mean, and absolute deviation (MAD), and coefficient variation (%CV). The two selectedmodels found to be optimal were the following: model one (V=a+bDBH²), established by employing DBH only; and model four (V=a+bDBH²H), established by utilizing DBH and height, respectively. The findings of non-linear regression indicated statistical significance (p < 0.05) in a and b, which were the coefficients for the intercepts and slopes of the models. The FI of the models ranged between 94% and 99%, and the bias was close to zero, while MAD ranged from 0.01 to 0.05, and %CV from 5.97 to 14.43, indicating a high level of fitness. Thus, using the suggested models, the basic information necessary for forest management was obtained, and an estimation of the stem volume was effected without delay soon after effecting DBH and height measurements.

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.162-172
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• 2002

Schizosaccharomyces pombe is suited for the study of cytokinesis as it divides by forming a septum in the middle of the cell at the end of mitosis. To enhance our understanding of the cytokinesis, we have carried out a genetic screen for temperature-sensitive S. pombe mutants that show defects in septum formation and cell division. Here we present the isolation and characterization of a new temperature-sensitive mutant, sun1(septum uncontrolled), which undergoes uncontrolled septation during cell division cycle at restrictive temperature $(37^{\circ}C)$. In sun1 mutant, actin ring and septum are positioned at random locations and angles, and nuclear division cycle continues. These observations suggest that the sun] gene product is required for the proper placement of the actin ring as well as precise septation. The sun] mutant is monogenic recessive mutation unlinked to previously known various cdc genes of S. pombe. In a screen for $sunl^+$ gene to complement the sun] mutant, we have cloned a gene, $susl^+$(suppressor of sun1 mutant), that encodes a protein of 689 amino acids. The predicted amino acid sequence of $susl^+$ gene is similar to the human hMadlp and Saccharomyces cerevisiae Mad1p, a component of the spindle checkpoint in eukaryotic cells. The null mutant of $susl^+$ gene grows normally at various temperatures and has the increased sensitivity to anti-microtubule drug, while $susl^+$ mutant shows no sensitivity to microtubule destabilizing drugs. The putative S. pombe Sus1p directly interacts with S. pombe Mad2p in yeast two-hybrid assays. These data suggest that the newly isolated susr gene encodes S. pombe Mad1p and suppresses sun] mutant defective in controlled septation in a cell division cycle.

• Parasites, Hosts and Diseases
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• v.57 no.2
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• pp.185-189
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• 2019

To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1/S- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the 2 stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. Transcriptome analysis of cells in 2 distinct cell cycle stages of G. lamblia confirmed previously reported components of cell cycle (PcnA, cyclin B, and CDK) and identified additional cell cycle components (NEKs, Mad2, spindle pole protein, and CDC14A). This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.

• ETRI Journal
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• v.18 no.2
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• pp.61-74
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• 1996

We propose two new rate control schemes to improve MPEG-2 rate control in view of visual quality when scene changes happen. Two proposed schemes are characterized by real-time and non real-time improvement to reduce the impact of scene changes. We also propose a new target-bit prediction method using spatial activity of pictures and present a simple and efficient scene change detection scheme using signed difference of mean absolute difference (MAD). Computer simulation results show that the proposed real-time algorithm effectively alleviates visual quality degradation after scene changes. The proposed non real-time algorithm gives maximum 2 dB improvement in peak signal-to-noise ratio (PSNR) at a scene-changed picture, compared with MPEG-2 rate control scheme and it shows better quality than the real-time one.