Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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RNA-sequencing Profiles of Cell Cycle-Related Genes Upregulated during the G2-Phase in Giardia lamblia

  • Kim, Juri (Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Shin, Mee Young (Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Park, Soon-Jung (Department of Environmental Medical Biology and Institute of Tropical Medicine, Yonsei University College of Medicine)
  • Received : 2019.02.15
  • Accepted : 2019.03.27
  • Published : 2019.04.30
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Abstract

To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1/S- or G2-phase by treatment with nocodazole and aphidicolin were prepared from the synchronized cell cultures. RNA-sequencing analysis of the 2 stages of Giardia cell cycle identified several cell cycle genes that were up-regulated at the G2-phase. Transcriptome analysis of cells in 2 distinct cell cycle stages of G. lamblia confirmed previously reported components of cell cycle (PcnA, cyclin B, and CDK) and identified additional cell cycle components (NEKs, Mad2, spindle pole protein, and CDC14A). This result indicates that the cell cycle machinery operates in this protozoan, one of the earliest diverging eukaryotic lineages.

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References

  1. Lane S, Lloyd D. Current trends in research in the waterborne parasite Giardia. Crit Rev Microbiol 2002; 28: 123-147. https://doi.org/10.1080/1040-840291046713
  2. Reiner DS, Ankarklev J, Troell K, Palm D, Bernander R, Gillin FD, Andersson JO, Svard SG. Synchronisation of Giardia lamblia: identification of cell cycle stage-specific genes and a differentiation restriction point. Int J Parasitol 2008; 38: 935-944. https://doi.org/10.1016/j.ijpara.2007.12.005
  3. Poxleitner MK, Dawson SC, Cande WZ. Cell cycle synchrony in Giardia intestinalis cultures achieved by using nocodazole and aphidicoliln. Eukayot Cell 2008; 7: 569-574. https://doi.org/10.1128/EC.00415-07
  4. Horlock-Roberts K, Reaume C, Dayer G, Ouellet C, Cook N, Yee J. Drug-free approach to study the unusual cell cycle of Giardia intestinalis. mSphere 2017; 2: e00384-00316.
  5. Bernander R, Palm JE, Svard SG. Genome ploidy in different stages of the Giardia lamblia life cycle. Cell Microbiol 2001; 3: 55-62. https://doi.org/10.1046/j.1462-5822.2001.00094.x
  6. Keister DB. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg 1983; 77: 487-488. https://doi.org/10.1016/0035-9203(83)90120-7
  7. Gargantini PR, Serradell MDC, Rios DN, Tenaglia AH, Lujan HD. Antigenic variation in the intestinal parasite Giardia lamblia. Curr Opin Microbiol 2016; 32: 52-58. https://doi.org/10.1016/j.mib.2016.04.017
  8. Gourguechon S, Holt LJ, Cande WZ. The Giardia cell cycle progresses independently of the anaphase-promoting complex. J Cell Sci 2013; 126: 2246-2255. https://doi.org/10.1242/jcs.121632
  9. Leung W, Baxley RM, Moldovan GL, Bielinsky AK. Mechanisms of DNA Damage Tolerance: Post-Translational Regulation of PCNA. Genes 2018; 10: E10. https://doi.org/10.3390/genes10010010
  10. Kawabe T, Suganuma M, Ando T, Kimura M, Hori H, Okamoto T. Cdc25C interacts with PCNA at G2/M transition. Oncogene 2002; 21: 1717-1726. https://doi.org/10.1038/sj.onc.1205229
  11. Naryzhny SN. Proliferating cell nuclear antigen: a proteomics view. Cell Mol Life Sci 2008; 65: 3789-3808. https://doi.org/10.1007/s00018-008-8305-x
  12. Kernan J, Bonacci T, Emanuele MJ. Who guards the guardian? Mechanisms that restrain APC/C during the cell cycle. Biochim Biophys Acta Mol Cell Res 2018; 1865: 1924-1933. https://doi.org/10.1016/j.bbamcr.2018.09.011
  13. Vicente JJ, Cande WZ. Mad2, Bub3, and Mps1 regulate chromosome segregation and mitotic synchrony in Giardia intestinalis, a binucleate protist lacking an anaphase-promoting complex. Mol Biol Cell 2014; 25: 2774-2787. https://doi.org/10.1091/mbc.e14-05-0975
  14. Fry AM, Bayliss R, Roig J. Mitotic Regulation by NEK Kinase Networks. Front Cell Dev Biol 2017; 5: 102. https://doi.org/10.3389/fcell.2017.00102
  15. Manning G, Reiner DS, Lauwaet T, Dacre M, Smith A, Zhai Y, Svard S, Gillin FD. The minimal kinome of Giardia lamblia illuminates early kinase evolution and unique parasite biology. Genome Biol 2011; 12: R66. https://doi.org/10.1186/gb-2011-12-7-r66

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