• Korean Journal of Animal Reproduction
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• v.27 no.3
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• pp.233-240
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• 2003

This study was conducted to examine the effects of oocyte maturation period, phytohemagglutinin-P (PHA-P) treatment and activation agent on the enucleation, fusion, activation or in vitro development of bovine nuclear transfer embryos. Bovine oocytes were enucleated at 16∼24 h of in vitro maturation (IVM). Adult ear skin cells treated or non-treated with PHA-P were transferred into enucleated oocytes. Reconstituted oocytes treated or non-treated with PHA-P were fused by a pulse of 1.5 kV/cm for 30 $\mu$sec. Fused oocytes were activated with a combination of calcium ionophore (A23187) and cycloheximide (CHXM) or dimethylaminopurine (DMAP), and cultured in vitro for 7∼9 days. Enucleation rate was significantly increased when oocytes were matured for 16∼18 h (70.2∼92.3%, P<0.05) compared to that of oocytes were matured for 20∼24 h (44.3∼53.4%). The location of metaphase-II plate was far off from the 1st polar body as maturation time was increased. PHA-P treatment of donor cells or reconstituted oocytes significantly improved fusion rate (P<0.05). Cleavage and blastocyst formation rates were significantly increased after activation with a combination of A23187 and DMAP (78.6% and 32.9%, respectively) compared to those of embryos activated with a combination of A23l87 and CHXM (48.5 and 15.2%, respectively). From the present result, it is suggested that high enucleation efficiency can obtained by using oocytes matured for 18 h. It also shows that PHA-P treatment can improve the fusion rate, and activation with a combination of A23187 and DMAP can enhance the embryo development.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.4
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• pp.238-250
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• 1993

Sixty two natural habitats of Korean lespedeza (Lespedeza stipulacea Maxim) were investigated to obtain basic data on the plant growth and the soil physico-chemical properties such as pH, mineral elements, organic matter content, soil separats and texture. Analyzed results can be summarized as follows. 1. Plant height and dry matter yield of the samples higher in late August and early September, although they varied greatly depending on the sampling date and location. 2. The plant shoot should higher contents of N, P, K. Ca and Mn than root and the plant root was higher in Mg, Fe, Cu, and Zn contents than shoot. 3. Soil pH and Ca content of the natural habitats were higher, but the contents of P$_2$O$_{5}$, K, Mg, Na and organic matter were lower than those of the average soil in Korea. The soil texture of natural habitats showed much higher portion of sand and extremely low portion of silt and clay 4. Korean lespedeza is well adapted to soils so low in fertility and organic matter that other crops and most weed fail. also it appears to thrive on the loamy sands, sandy loams of the piedmont region and on similar soils with variously physico-chemical properties.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.28 no.6
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• pp.579-586
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• 2010

Due to the recent development of measuring technology and 3D programs, it has become possible to obtain various spatial data. This study utilizes the 2-dimensional data and 3-dimensional data extraction technology based on the existing empirical and statistical DB. The data obtained from geospatial data technology are integrated with civil engineering BIM to conduct the modeling of the topography of the target region and select the optimum location condition by using the cut and fill balance of the volume of earth. The target area is the land around Tamjin River, Jangheong-gun, Jeolla-do. The 3-dimensional topology linked with 3-dimensional mapping technology by using the orth-image and aerial LiDAR that uses aerial photo of the target area is visualized with Civil3D of AutoDesk. By using Civil3D program, the Thanks to the recent development of measuring technology and 3D programs, target area is analyzed through visualization and related data can be obtained for analysis. The method of using civil engineering BIM enables to obtain various and accurate information about the target area which is helpful for addressing the issues risen from the existing methodology. In this regard, it aims at searching for the alternatives and provides suggestions to utilize the information.

• Journal of Navigation and Port Research
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• v.31 no.6
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• pp.479-484
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• 2007

For calculation of wave field for design of coastal and port structures, generally the wind fields from inland observation record or the predicted waves from deep water wave transformation model are being used. However, for the first case, as we should revise the wave data adopting correcting parameters depending on the distance from the coast and location, it is difficult to extract water waves from wind field. Furthermore, for the second case, because of the calculation which executed under very large grid sizes in the wide domain, the simulation(wave transformation) implied uncertainty in the near shore area and shallow region. So it's difficult to obtain exact data from the simulation. Thus, in this study the calculation of wave field on shallow water is accomplished using the observed data of typhoon 'Maemi' in the Korea Eastern South sea. Moreover, for the accuracy of the calculated wave field, we compared and studied the observed data of wave height and direction on the vicinity of the Ulsan waters. It is proved that the results of this study is more accurate than the existing method with showing ${\pm}1.3%$ difference between observed and calculated wave height distribution in Ulsan waters

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.3
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• pp.1889-1894
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• 2013

Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.

• Journal of Korean Society of Forest Science
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• v.28 no.1
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• pp.43-49
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• 1975

These studies were carried out to know the variation of seeds and top height growth of seedlings by locations along the southern sea coast in Korea. The results obtained were summarized as follows: 1. The number of seeds per cone was showed to be the highest in Chung-mu and the lowest in Wul-jin. 2. The variations of seed length and seed width were showed to be high in Wul-jin and Young-duck along the eastern sea and Bul-kyo, Mok-po along the southern sea. 3. The number of cotyledons was not correlations between seed length and seed width. And the number of cotyledon was showed to be 7 leaves ie. all locations. 4. The results of T-test were not significant by all locations. 5. By the locations, the results of analysis of variance were not significant in top height growth of seedlings by sowing of the present year, but high significant in July of the next year. In conclusion, the number of seeds per cone was showed to be high in the variation of seed characters and the top height growth of seedlings was showed to be significant in sowing of the next year.

• Journal of the Institute of Electronics and Information Engineers
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• v.49 no.12
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• pp.73-80
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• 2012

In this article, we propose a P2P-based mobility management protocol for global seamless handover in heterogeneous wireless networks. Unlike previous mobility management protocols such as IETF MIPv4/6 and its variants, the proposed protocol can support global seamless handover without changing the existing network infrastructure. The idea of the proposed protocol is that the location management function for mobility management is separately supported from packet forwarding function, and bidirectional IP tunnels for packet transmission are dynamically constructed between two end-to-end mobile hosts. In addition, early handover techniques have been developed to avoid large handover delays and packet losses using the IEEE 802.21 Media Independent Handover functions. The architecture and signaling procedure of the proposed protocol have been designed in detail, and the mathematical analysis and simulation have been done for performance evaluation. The performance results show that the proposed protocol outperforms the existing MIPv6 and HMIPv6 in terms of handover latency and packet loss.

• Journal of the Korean Society of Marine Environment & Safety
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• v.22 no.5
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• pp.547-553
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• 2016

Black carbon (BC) contained in particulate matter (PM) originating from the exhaust gases of ships' diesel engines has been receiving great attention as a cause of glacial melting and warming in the polar regions. In this study, we took samples from various locations of PM emitted from the training ship (T/S) HANBADA's main engine, in cooperation with the Korea Maritime and Ocean University. We analyzed the structure and characteristics of these samples using high-resolution transmission electron microscopy (HR-TEM) and applied our findings as fundamental research for developing PM reduction technology. We also employed our results to determine appropriate preemptive action to meet upcoming PM/BC regulations. In addition, we confirmed the emission trend of pollutants from exhaust gases under various engine operating conditions using an exhaust gas analyzer. Results obtained from the analysis of HR-TEM images showed that the structure of the PM is chain-like wispy agglomerates consisting of a number of individual spherical particles. As the sampling location was moved away from the turbo charger (T/C) towards the funnel, more condensates were observed at a low temperature and the molecular structure of the PM lost its characteristic BC structure as an amorphous structure gradually appeared. Furthermore, through the analysis of exhaust gases, we predicted a decrease in PM concentration in the exhaust stream as engine rpm increase.

• Spatial Information Research
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• v.18 no.4
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• pp.65-73
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• 2010

Recently, the importance of the ocean has been increasing internationally as the new source for mineral resources following the exhausted land resources that arc becoming scarce. On a long-term aspect, growth of nations by gaining competitiveness on marine resources was considered a paradigm. Because dominating the development right of marine resources came up as the main concern. South Korea has also been interested in marine resources and this is the reason why massive amounts of marine exploration data arc annually created through surveying and drilling around the Korean Peninsula. but the data has not been systematically managed very well because of its economic costs. Therefore, this research is mainly focused on systematical data managing methods. For Systematical data management. the exploration data is integrated and organized by using XML tables. This can be a systematical data management. because the methods release dependency between data and system, and it also enables to update existing data and renew the data. In the future, the constructed database from this study could definitely contribute to enhancing data management. As well, the developed system in this research can provide various spatial analysis and searching techniques to enable easier data provision of various exploration areas. Furthermore. this will be very useful to extend functions of the system and to adopt other types of DBMS. In addition, the spatial analysis and search function of location based service can be utilized through GIS. and it can support sustainable and systematic management in a long term.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.12
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• pp.5089-5094
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• 2015

FADS1 (fatty acid desaturase 1) plays a crucial role in fatty acid metabolism, and it was recently reported to be involved in tumorigenesis. However, the role of FADS1 expression in esophageal squamous cell carcinoma (ESCC) remains unknown. In the current study, we investigated the expression and clinical pathologic and prognostic significance of FADS1 in ESCC. Immunohistochemical analyses revealed that 58.2% (146/251) of the ESCC tissues had low levels of FADS1 expression, whereas 41.8% (105/251) exhibited high levels of FADS1 expression. In positive cases, FADS1 expression was detected in the cytoplasm of cells. Correlation analyses demonstrated that FADS1 expression was significantly correlated with tumor location (p=0.025) but not with age, gender, histological grade, tumor status, nodal status or TNM staging. Furthermore, patients with tumors expressing high levels of FADS1had a longer disease-free survival time (p<0.001) and overall survival time (p <0.001). Univariate and multivariate analyses revealed that, along with nodal status, FADS1 expression was an independent and significant predictive factor (p<0.001). In conclusion, our study suggested that FADS1 might be a valuable biomarker and potential therapeutic target for ESCC.