• 한국임상수의학회지
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• 제28권4호
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• pp.394-398
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• 2011

Mesenchymal stem cells (MSC) are pluripotent cells that can be found in umbilical cord blood from new borne babies as well as placenta, bone marrow, adipose tissue, amniotic fluid, muscle, et al. MSC are capable of renewing themselves without differentiation in long-term culture, also can be differentiated into various tissues under specific condition. Formulating a cryopreservation protocol for the MSC is required because these cells cannot survive for long periods under in vitro culture conditions and a new formulation of harmless cryoprotectant is needed for the direct injection of MSC into patients. The undifferentiated MSC were frozen with a vitrification solution of 40% ethylene glycol, 20% Ficoll-70 and 0.3M sucrose. The survival rate after thawing and their proliferation rate were examined and compared with slow rate cooling methods using dimethylsulfoxide (DMSO). The vitrification method showed high survival rate after thawing and proliferation capacity comparable to DMSO. It can be suggested that ultra-rapid cooling method by vitrification is reliable methods for long term preservation of MSC and the vitrification solution with ethylene glycol, Ficoll-70 and sucrose will be more beneficially used for direct transplantation of MSC into patients than DMSO solution.

• 대한공간정보학회지
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• 제17권2호
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• pp.55-60
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• 2009

본 연구에서는 경주시 내남면을 대상으로 KOMPSAT-2 MSC(Multi Spectral Camera) 영상(2007.06.12)을 기반으로 NDVI(Normalized Difference Vegetation Index)와 TCT(Tasseled-Cap Transformation) 영상 알고리즘을 적용하여 DN 분포도를 작성 하였다. NDVI 및 TCT DN 분포도와 산림 현장 조사 결과와의 비교 분석을 통하여 임상 분류 정확도 향상을 위한 영상 알고리즘 변별력 분석을 수행하고 마지막으로 현장조사 자료와의 중첩 분석을 통하여 임상분류 정확성을 검증 하였다. 본 연구를 통하여 KOMPSAT-2 MSC 영상을 이용하여 임상 분류 자동화 실용성에 대한 검토와 정밀 산림 임상도 제작과정에서 저비용 고효율성을 기대할 수 있으리라 사료된다.

• 한국정보처리학회논문지
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• 제6권1호
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• pp.193-202
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• 1999

음성뿐만 아니라 데이터, 화상 및 영상 등의 고품질 서비스를 제공하는 제 3세대 이동통신(IMT-2000 : International Mobile Telecommunications-2000)은 2000년대 초에 전체 통신 서비스의 50% 정도를 점유할 것으로 예견된다. 한편 개인의 이동성을 보장할 수 있는 UPT(Universal Personal Telecommunications) 서비스는 다양한 종류의 유무선망을 단일 번호로써 을 수 있는 중요한 서비스로 인식되고 있다. 본 논문은 차세대 지능망 서비스를 지원하는 IMT-2000 교환기의 시스템 구조 및 소프트웨어 블록 형상을 제안하고 지능망 기능 지원에 필요한 교환기 내부의 서비스 교환 기능을 설계한다. 또한 제안된 구조와 서비스 교환 기능을 적용하여 차세대 지능망 서비스의 대표적인 서비스인 UPT 서비스의 호 시나리오를 설계한다. 특히 IMT-2000 교환기 내부 소프트웨어 블록간 호 흐름에 바탕을 둔 UPT 서비스기능 구현에 초점을 두어 UPT 서비스 호 시나리오를 설계한다.

• 동의생리병리학회지
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• 제20권1호
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• pp.181-186
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• 2006

A pharmacological inhibition of nitric oxide synthase (NOS) in rats produces vasoconstriction, renal dysfunction, and hypertension. The present study was aimed at investigating whether the methanol extract of Serous commixta cortex (MSC) ameliorates $N^G$-nitro-L-arginine methylester (L-NAME) induced hypertension in rats. Treatment of rats with L-NAME (10 mg/kg/day in drinking water, 5 weeks) caused a sustained increase in systolic blood pressure (SBP). Administration of MSC (100 or 200 mg/kg/day, p.o) significantly lowered the SBP in the L-NAME-treated rats and this effect was maintained throughout the whole experimental period. Moreover, ecNOS expression in aorta and kidney tissue from L-NAME treated rats was significantly restored dy administration of MSC. Furthermore, the impairment of acetylcholine (ACh)-induced relaxation of aortic rings in the L-NAME treated rats was reversed dy administering of MSC. The renal functional parameters including urinary volume, sodium excretion, and creatinine clearance (Ccr) were also restored by administering MSC. Taken together, the present study suggeststhat MSC prevents the increase in SBP in rats with L-NAME-induced hypertension, which may result from the up-regulation of the vascular and renal ecNOS/No system.

• 대한불안의학회지
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• 제18권1호
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• pp.10-16
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• 2022

Objective : The present study examined the effectiveness of the mindful self-compassion (MSC) program on anxiety, depression, self-compassion and emotion regulation. Methods : A total of 29 subjects (mean age 27.5±6.5 years, 15 males and 14 females) participated in a standardized 8-week MSC program. The control group consisted of age- and sex-matched twenty participants (mean age 26.0±2.9 years, 11 males and 9 females). All subjects completed self-report measurements at two weeks before and after the MSC program. Results : MSC training improved self-compassion as demonstrated by the significant group x time interaction effects on the total Self-Compassion Scale scores (F[1, 47]=8.324, p<0.01). Regarding the subscale scores, a significant improvement in self-kindness, isolation and mindfulness components of self-compassion was observed after MSC training. A significant group x time interaction was observed on the self-kindness subscale (F[1, 47]=4.664, p<0.05), with a significant main effect of time (F[1, 47]=23.723, p<0.001). The isolation subscale showed a significant group x time interaction (F[1, 47]=8.698, p<0.001). For the mindfulness subscale, there was a significant group x time interaction (F[1, 47]=6.611, p<0.05) and main effect of time (F[1, 47]=6.611, p<0.05). MSC training also improved the acceptance emotion regulation strategy, as demonstrated by the significant group x time interaction in the acceptance subscale scores of the Cognitive Emotion Regulation Questionnaire (F[1, 47]=6.845, p<0.05). Conclusion : MSC training showed efficacy in fostering self-compassion and improving emotion regulation. Thus, this program might be applicable to improve mental health.

• 한국항공운항학회:학술대회논문집
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• 한국항공운항학회 2007년도 추계학술대회
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• pp.93-96
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• 2007

• 생명과학회지
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• 제28권9호
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• pp.1042-1047
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• 2018

홍조류인 꼬물꼬시래기(Gracilaria vermiculophylla)는 전 세계의 해변 지역에 널리 퍼져 있으며 아시아 국가에서 식량 자원으로 이용되어왔다. 이전 연구에 따르면, Gracilaria 속 홍조류 추출물에서 항산화 및 항염증 효과가 보고 되었다. 본 연구에서는 노화된 인간의 골수 유래 중간엽 줄기세포(hBM-MSCs)를 이용하여 Gracilaria vermiculophylla 추출물(GV-Ex)의 항노화 효과를 조사하였다. MTT 분석와 immunoblot 분석(apoptotic protein p53과 cleaved caspase-3)을 이용하여, GV-Ex 전처리는 산화적 스트레스에 의해 손상된 hBM-MSCs의 세포생존력을 향상시킴을 확인하였다. 또, 세포내 생성된 ROS는 장기간 배양 된 MSCs (Passages 17; P-17)와 P-7 MSC에서 측정하여 서로 비교하였는데, P-17 MSC에서 증가되었고, GV-Ex 처리하면(GV-Ex treated P-17 MSCs) 유의하게 감소되었다. 또한, 항산화 효소인 SOD1와 SOD2, CAT의 발현 역시 GV-Ex 처리함에 따라 복원됨을 관찰하였다. 노화 표지단백질인 p53와 p21, p16 등의 발현 또한 GV-Ex를 처리한 P-17 MSC에서 감소되었다. 줄기세포의 골세포(osteocytes) 혹은 지방세포(adipocytes)로 분화하는 능력 역시 GV-Ex를 처리한 P-17 MSCs에서 개선되었다. 이상과 같은 결과를 통해, GV 추출물은 노화된 줄기세포의 기능을 개선함을 시사한다.

• IMMUNE NETWORK
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• 제23권6호
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• pp.48.1-48.21
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• 2023

Mesenchymal stromal/stem cells (MSCs) possess immunoregulatory properties and their regulatory functions represent a potential therapy for acute lung injury (ALI). However, uncertainties remain with respect to defining MSCs-derived immunomodulatory pathways. Therefore, this study aimed to investigate the mechanism underlying the enhanced effect of human recombinant bone morphogenic protein-2 (rhBMP-2) primed ES-MSCs (MSC^BMP2) in promoting Tregs in ALI mice. MSC were preconditioned with 100 ng/ml rhBMP-2 for 24 h, and then administrated to mice by intravenous injection after intratracheal injection of 1 mg/kg LPS. Treating MSCs with rhBMP-2 significantly increased cellular proliferation and migration, and cytokines array reveled that cytokines release by MSC^BMP2 were associated with migration and growth. MSC^BMP2 ameliorated LPS induced lung injury and reduced myeloperoxidase activity and permeability in mice exposed to LPS. Levels of inducible nitric oxide synthase were decreased while levels of total glutathione and superoxide dismutase activity were further increased via inhibition of phosphorylated STAT1 in ALI mice treated with MSC^BMP2. MSC^BMP2 treatment increased the protein level of IDO1, indicating an increase in Treg cells, and Foxp3⁺CD25⁺ Treg of CD4⁺ cells were further increased in ALI mice treated with MSC^BMP2. In co-culture assays with MSCs and RAW264.7 cells, the protein level of IDO1 was further induced in MSC^BMP2. Additionally, cytokine release of IL-10 was enhanced while both IL-6 and TNF-α were further inhibited. In conclusion, these findings suggest that MSC^BMP2 has therapeutic potential to reduce massive inflammation of respiratory diseases by promoting Treg cells.

• International Journal of Stem Cells
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• 제16권2호
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• pp.191-201
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• 2023

Background and Objectives: O-cyclic phytosphingosine-1-phosphate (cP1P) is a synthetic chemical and has a structure like sphingosine-1-phosphate (S1P). S1P is known to promote cell migration, invasion, proliferation, and anti-apoptosis through hippocampal signals. However, S1P mediated cellular-, molecular mechanism is still remained in the lung. Acute lung injury (ALI) and its severe form acute respiratory distress syndrome (ARDS) are characterized by excessive immune response, increased vascular permeability, alveolar-peritoneal barrier collapse, and edema. In this study, we determined whether cP1P primed human dermal derived mesenchymal stem cells (hdMSCs) ameliorate lung injury and its therapeutic pathway in ALI mice. Methods and Results: cP1P treatment significantly stimulated MSC migration and invasion ability. In cytokine array, secretion of vascular-related factors was increased in cP1P primed hdMSCs (hdMSC^cP1P), and cP1P treatment induced inhibition of Lats while increased phosphorylation of Yap. We next determined whether hdMSC^cP1P reduce inflammatory response in LPS exposed mice. hdMSC^cP1P further decreased infiltration of macrophage and neutrophil, and release of TNF-α, IL-1β, and IL-6 were reduced rather than naïve hdMSC treatment. In addition, phosphorylation of STAT1 and expression of iNOS were significantly decreased in the lungs of MSC^cP1P treated mice. Conclusions: Taken together, these data suggest that cP1P treatment enhances hdMSC migration in regulation of Hippo signaling and MSC^cP1P provide a therapeutic potential for ALI/ARDS treatment.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2005년도 Proceedings of ISRS 2005
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• pp.586-588
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• 2005

The MSC (Multi Spectral Camera) system is a remote sensing payload to obtain high resolution ground image. This system uses lossy image compression method for &Direct mission& that transmit whole image during one contact. But some image degradation occurred especially at high compression ratio. To reduce this degradation, the MSC uses NUC (Non-uniformity Correction) Unit. This unit correct CCD (Charge Coupled Device)'s high-frequency non-uniformity. So high frequency contents of image can be minimized and whole system SNR can be maximized. But NUC has some disadvantage either. It decreases entire system reliability by adding one electronic system. Adding NUC also led to difficulty of electronic design, assembly and testability. In this paper, the comparison is performed between on-orbit non-uniform correction and on ground correction. by evaluating NUC advantage for the point of view of image quality. Using real MSC parameter and proper model, considerable reference point for the system design came to possible.