Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Cryopreservation of Mesenchymal Stem Cells by Vitrification

중간엽줄기세포의 초자화 동결법에 의한 냉동보존

  • Lee, Hyo-Jong (Institute of Animal Medicine, College of Veterinary Medicine) ;
  • kang, Sun-Young (Gyeongnam Wild Life Center) ;
  • Park, Se-Jin (Institute of Animal Medicine, College of Veterinary Medicine) ;
  • Lee, Seung-Yong (Institute of Animal Medicine, College of Veterinary Medicine) ;
  • Lee, Hee-Chun (Institute of Animal Medicine, College of Veterinary Medicine) ;
  • Koh, Phil-Ok (Institute of Animal Medicine, College of Veterinary Medicine) ;
  • Park, Ji-Kwon (School of Medicine, Gyeongsang National University) ;
  • Paik, Won-Young (School of Medicine, Gyeongsang National University) ;
  • Yeon, Seong-Chan (Institute of Animal Medicine, College of Veterinary Medicine)
  • 이효종 (경상대학교 수의과대학 동물의학연구소) ;
  • 강선영 (경상남도 야생동물센터) ;
  • 박세진 (경상대학교 수의과대학 동물의학연구소) ;
  • 이승용 (경상대학교 수의과대학 동물의학연구소) ;
  • 이희천 (경상대학교 수의과대학 동물의학연구소) ;
  • 고필옥 (경상대학교 수의과대학 동물의학연구소) ;
  • 박지권 (경상대학교 의과대학) ;
  • 백원영 (경상대학교 의과대학) ;
  • 연성찬 (경상대학교 수의과대학 동물의학연구소)
  • Accepted : 2011.08.09
  • Published : 2011.08.30
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Abstract

Mesenchymal stem cells (MSC) are pluripotent cells that can be found in umbilical cord blood from new borne babies as well as placenta, bone marrow, adipose tissue, amniotic fluid, muscle, et al. MSC are capable of renewing themselves without differentiation in long-term culture, also can be differentiated into various tissues under specific condition. Formulating a cryopreservation protocol for the MSC is required because these cells cannot survive for long periods under in vitro culture conditions and a new formulation of harmless cryoprotectant is needed for the direct injection of MSC into patients. The undifferentiated MSC were frozen with a vitrification solution of 40% ethylene glycol, 20% Ficoll-70 and 0.3M sucrose. The survival rate after thawing and their proliferation rate were examined and compared with slow rate cooling methods using dimethylsulfoxide (DMSO). The vitrification method showed high survival rate after thawing and proliferation capacity comparable to DMSO. It can be suggested that ultra-rapid cooling method by vitrification is reliable methods for long term preservation of MSC and the vitrification solution with ethylene glycol, Ficoll-70 and sucrose will be more beneficially used for direct transplantation of MSC into patients than DMSO solution.

Keywords

References

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