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Development of drought Tolerant Temperate Rice Variety by Pyramiding QTLs, Pup1 and DTY4.1

  • Jae-Hyuk Han;Na-Hyun Shin;Ian Paul Navea;Jin-Woo Lee;IL-Ryong Choi;Joong Hyoun Chin
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.206-206
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    • 2022
  • Sustainable agriculture is a potential strategy to enable agricultural cultivation systems to feed the growing population under climate change. Sustainable agriculture consists of environment-friendly farming methods that allow the production of crops with minimal harm to the ecosystem. Early establishment in rice might be helpful to adopt sustainable agriculture with less inputs, such as water and phosphorus fertilizer. Two QTLs conferring tolerance to abiotic stress and low nutrition condition, DTY4.1 and Pup1, respectively, are effective for good establishment in the early growth stage under low water and phosphorus fertilizer application. We developed 'Sechanmi' and 'MSI 1-DTY' harboring Pup1 and DTY4.1 into MS11, a japonica rice variety adaptable to tropical regions, using Marker-Assisted Backcrossing (MABC). MS 11-PD lines were developed to meet the demand for less water and P fertilizer application throughout the growth stage of rice. In the F5 generation, water-saving or rainfed cultivation was performed in different P (phosphorus) content. Irrigation was applied only when severe drought was observed one month after transplanting. There was no significant difference observed between the parents and MS11-PD lines in low P conditions. However, MS11-PD lines had more tillers in P-supplied conditions compared to that of the parents 40 and 50 days after transplanting. Under the same amount of P, MS11-PD lines might have higher phosphorus uptake capacity than the parents, increasing the number of tillers and showing better early establishment. The better vegetative growth stage is one of the factors that can potentially increase production by way of higher number of panicles. Through this breeding strategy, it is possible to attain sustainable agriculture by applying less P and water to address the need of a growing population.

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MLC Sequencing File을 이용한 IMRT의 독립적 선량계산에 관한 연구

  • Sin, Dong-Ho;Park, Seong-Yong;Kim, Ju-Yeong;Kim, Dae-Yong;Jo, Gwan-Ho
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2005.04a
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    • pp.75-78
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    • 2005
  • IMRT를 이용한 환자치료에서 환자의 특이적 품질관리에 대한 독립적인 선량계산을 위해 치료계획용 컴퓨터로 부터 계획된 다엽콜리메이터의 배열파일(MLC sequencing file)과 계획된 MU값 및 선량계산 위치에서의 각각의 조사필드 별 기여 치 등을 얻어낸 후 MS office Excel 2000 VBA를 이용하여 간단한 분석을 통하여 MU값을 계산한 결과 계획선량과 계산선량이 ${\pm}$4% 의 선량 오차를 얻을 수 있었다.

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A Method of Recovery for Damaged ZIP Files (손상된 ZIP 파일 복구 기법)

  • Jung, Byungjoon;Han, Jaehyeok;Lee, Sang-jin
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.27 no.5
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    • pp.1107-1115
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    • 2017
  • The most commonly used PKZIP format is a ZIP file, as well as a file format used in MS Office files and application files for Android smartphones. PKZIP format files, which are widely used in various areas, require structural analysis from the viewpoint of digital forensics and should be able to recover when files are damaged. However, previous studies have focused only on recovering data or extracting meaningful data using the Deflate compression algorithm used in ZIP files. Although most of the data resides in compressed data in the ZIP file, there is also forensically meaningful data in the rest of the ZIP file, so you need to restore it to a normal ZIP file format. Therefore, this paper presents a technique to recover a damaged ZIP file to a normal ZIP file when given.

Software Security Testing using Block-based File Fault Injection (블록 기반 파일 결함 주입 기법을 이용한 소프트웨어 보안 테스팅)

  • Choi, Young-Han;Kim, Hyoung-Chun;Hong, Soon-Jwa
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.17 no.4
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    • pp.3-10
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    • 2007
  • In this paper, we proposed the methodology for security testing using block-based file fault injection. When fault is inserted into software, we consider the format of file in order to efficiently reduce the error that is caused by mismatch of format of file. The Vulnerability the methodology focuses on is related to memory processing, such as buffer overflow, null pointer reference and so on. We implemented the automatic tool to apply the methodology to image file format and named the tool ImageDigger. We executed fault-injection focused on WMF and EMF file format using ImageDigger, and found 10 DOS(Denial of Service) in Windows Platform. This methodology can apply to block-based file format such as MS Office file.

Development of Urban Flood Warning System Using Regression Analysis (회귀분석에 의한 도시홍수 예보시스템의 개발)

  • Lee, BeumHee
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.4B
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    • pp.347-359
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    • 2010
  • A simple web-based flood forecasting system using data from stage and rainfall monitoring stations was developed to solve the difficulty that real-time forecasting model could not get the reliabilities because of assumption of future rainfall duration and intensity. The regression model in this research could forecast future water level of maximum 2 hours after using data from stage and rainfall monitoring stations in Daejeon area. Real time stage and rainfall data were transformed from web-sites of Geum River Flood Control Office & Han River Flood Control Office based MS-Excel 2007. It showed stable forecasts by its maximum standard deviation of 5 cm, means of 1~4 cm and most of improved coefficient of determinations were over 0.95. It showed also more researches about the stationarity of watershed and time-series approach are necessary.

Gene Transformation of Ailanthus altissima Swingle by Agrobacterium tumefaciens (외래유전자(外來遺傳子)에 의(依)한 가중나무의 형질전환(形質轉換))

  • Park, Young Goo;Huh, Kyung;Choi, Myung Suk
    • Current Research on Agriculture and Life Sciences
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    • v.10
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    • pp.137-145
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    • 1992
  • An efficient transformation system was established for Ailanthus altissima utilizing the binary system of A. tumefaciens strain LBA4404. Callus was initiated from small portions of cambium tissue of A. altissima in vitro. Optimum regeneration was achieved with Murashige and Skoog(MS) medium containing 0.01mg/${\ell}$ 2, 4-D, 0.5mg/${\ell}$ BAP, 3%(w/v) sucrose and 0.75% agar. The multiplication of explants remarkably showed up on medium containing 1.0mg/${\ell}$ BAP. Leaf discs or internodal stem segments were inoculated with A. tumefaciens strain LBA 4404 containing the binary vector pPMB 101, which has both ${\beta}$-glucuronidase (GUS) marker gene and neomycin phosphotransferase II (NPT II) gene. Shoots had been regenerated from 24 lines out of inoculative 50 lines. Transformants were selected by their ability to grow on medium containing kanamycin sulphate (100mg/${\ell}$). Putative transformation was confirmed by GUS assays. Five GUS-positive plantlets were obtained which confirmed that this marker gene has been transferred into A. altissima.

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Quantitative Analysis of Pentachlorophenol in Paper Products (종이재 중의 Pentachlorophenol 정량 분석)

  • Hahn, Airan;Park, Jihye;Choi, Hyeongki;Hong, Sungyeap;Han, Hyechul;Jo, Byoungmuk;Oh, Jungsoo
    • Applied Chemistry for Engineering
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    • v.16 no.3
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    • pp.323-327
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    • 2005
  • Pentachlorophenol (PCP) is a representative compound of chlorophenol derivatives which are especially toxic and potentially carcinogenic compounds in the priority pollutant list of the EPA (Environmental Protection Agency, USA). The aim of this study was to investigate an analysis method of PCP in infinitesimal quantity retained in paper. PCP in paper was pretreated by solid phase extraction (SPE) in a phenyl cartridge. The extracted sample was acetylated or silylated and analyzed with GC/MS-SIM. The detection sensitivity of silylated PCP was improved significantly when compared with those of PCP itself and acetylated PCP. As a test specimen, an office paper and a wallpaper were used.

Erasing Methods for Improved Endurance Characteristics in Single-Poly EEPROM (단층 다결정실리콘 EEPROM의 Endurance 특성 개선을 위한 소거방법)

  • Yu, Yeong-Cheol;Jang, Seong-Jun;Yu, Jong-Geun;Lee, Gwang-Yeop;Kim, Yeong-Seok;Park, Jong-Tae
    • Journal of the Korean Institute of Telematics and Electronics D
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    • v.36D no.6
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    • pp.21-27
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    • 1999
  • In this work, single-poly EEPROM was designed and fabricated by using standard $0.8{\mu}m$ CMOS process. From the results of programming and erasing characteristics, it was found that the programming time was smaller than 10ms and the erasing time was about 100ms. To reduce the erasing time, several erasing methods were performed. The S/D erasing method was used to improve the endurance characteristics which was degraded due to electron trapping in programming and erasing cycles. By using the S/D erasing method, the more improved endurance characteristics then conventional one was obtained.

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Development of a Simultaneous Detection and Quantification Method of Anorectics in Human Urine Using GC-MS and its Application to Legal Cases (GC-MS를 이용한 사람 뇨시료 중 비만치료제 분석 및 비만치료제 남용 현황의 법과학적 고찰)

  • Choi, Hyeyoung;Lee, Jaesin;Jang, Moonhee;Yang, Wonkyung;Kim, Eunmi;Choi, Hwakyung
    • YAKHAK HOEJI
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    • v.57 no.6
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    • pp.420-425
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    • 2013
  • Phentermine (PT) and phenmetrazine (PM) have been widely used as anti-obesity drugs. These drugs should be used with caution due to its close relation to amphetamine in its structure and toxicity. PT and PM, amphetamine-type anorectics, have recently been considered as alternatives for methamphetamine abuse in Korea. In addition, the misuse and abuse of PT and PM obtained by illegal sources such as the internet become a serious social problem. In the present study, a simultaneous detection and quantification method for determining PT and PM in human urine was developed and validated according to the international guidelines. The urine samples were screened using a fluorescence polarization immunooassay and analyzed by gas chromatography mass spectrometry (GC-MS) after extraction using automatic solid phase extraction (SPE) with a mixed-mode cation exchange cartridge and derivatization with pentafluoropropionic anhydride (PFPA). The validation results for selectivity, linearity, limits of detection (LOD) and quantification (LOQ), intra- and inter-assay precision and accuracy and recovery were satisfactory. The validated method was successfully applied to authentic urine samples collected from 38 drug abuse suspects. PT and/or PM were identified with or without methamphetamine in urine samples. Abuse of PT and PM have increased continuously in Korea, therefore, closer supervision of the inappropriate use of anoretics is necessary.

High-frequency regeneration of plants in vitro from seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim

  • Kim, Tae-Dong;Kim, Nam-Ho;Park, Eung-Jun;Lee, Na-Nyum
    • Journal of Plant Biotechnology
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    • v.48 no.1
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    • pp.54-61
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    • 2021
  • This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5 ~ 1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.