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xCyp26c Induced by Inhibition of BMP Signaling Is Involved in Anterior-Posterior Neural Patterning of Xenopus laevis

  • Yu, Saet-Byeol;Umair, Zobia;Kumar, Shiv;Lee, Unjoo;Lee, Seung-Hwan;Kim, Jong-Il;Kim, SungChan;Park, Jae-Bong;Lee, Jae-Yong;Kim, Jaebong
    • Molecules and Cells
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    • v.39 no.4
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    • pp.352-357
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    • 2016
  • Vertebrate neurogenesis requires inhibition of endogenous bone morphogenetic protein (BMP) signals in the ectoderm. Blocking of BMPs in animal cap explants causes the formation of anterior neural tissues as a default fate. To identify genes involved in the anterior neural specification, we analyzed gene expression profiles using a Xenopus Affymetrix Gene Chip after BMP-4 inhibition in animal cap explants. We found that the xCyp26c gene, encoding a retinoic acid (RA) degradation enzyme, was upregulated following inhibition of BMP signaling in early neuroectodermal cells. Whole-mount in situ hybridization analysis showed that xCyp26c expression started in the anterior region during the early neurula stage. Overexpression of xCyp26c weakly induced neural genes in animal cap explants. xCyp26c abolished the expression of all trans-/cis-RA-induced posterior genes, but not basic FGF-induced posterior genes. Depletion of xCyp26c by morpholino-oligonucleotides suppressed the normal formation of the axis and head, indicating that xCyp26c plays a critical role in the specification of anterior neural tissue in whole embryos. In animal cap explants, however, xCyp26c morpholinos did not alter anterior-to-posterior neural tissue formation. Together, these results suggest that xCyp26c plays a specific role in anterior-posterior (A-P) neural patterning of Xenopus embryos.

Design of a Ultra Miniaturized Voltage Tuned Oscillator Using LTCC Artificial Dielectric Reson (LTCC 의사 유전체 공진기를 이용한 초소형 전압제어발진기 설계)

  • Heo, Yun-Seong;Oh, Hyun-Seok;Jeong, Hae-Chang;Yeom, Kyung-Whan
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.23 no.5
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    • pp.613-623
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    • 2012
  • In this paper, we present an ultra miniaturized voltage tuned oscillator, with HMIC-type amplifier and phase shifter, using LTCC artificial dielectric resonator. ADR which consists of periodic conductor patterns and stacked layers has a smaller size than a dielectric resonator. The design specification of ADR is obtained from the design goal of oscillator. The structure of the ADR with a stacked circular disk type is chosen. The resonance characteristic, physical dimension and stack number are analyzed. For miniaturization of ADRO, the ADR is internally implemented at the upper part of the LTCC substrate and the other circuits, which are amplifier and phase shifter are integrated at the bottom side respectively. The fabricated ADRO has ultra small size of $13{\times}13{\times}3mm^3$ and is a SMT type. The designed ADRO satisfies the open-loop oscillation condition at the design frequency. As a results, the oscillation frequency range is 2.025~2.108 GHz at a tuning voltage of 0~5 V. The phase noise is $-109{\pm}4$ dBc/Hz at 100 kHz offset frequency and the power is $6.8{\pm}0.2$ dBm. The power frequency tuning normalized figure of merit is -30.88 dB.

Study of Failure Examples for Emission Gas Control System in Gasoline Engine (가솔린 엔진 배출가스 제어장치에 대한 고장사례 고찰)

  • Lee, Il Kwon;Lee, Jong Ho;Lee, Young Suk;Youm, Kwang Wook;han, Jae Oh;Lim, Ha young
    • Journal of the Korean Institute of Gas
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    • v.20 no.6
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    • pp.37-42
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    • 2016
  • The purpose of this paper is to study for the emission gas control of passenger car. The first example, the PCSV never open when operating condition, but it opened by causing malfunction because of trouble. As a result, the purge gas entered into surge tank, a mount of fuel was displayed with excessive supply on tester. Therefore, it certified the bad-condition of the engine when idling by decreasing of fuel injection quantity from engine ECU. The second example, the hose activating a EGR valve didn't supply the vacuum pressure because of assembling the other part. Thus, it knew the bad-condition of engine that the EGR valve would not work normally by leaking with the other port. The third example, as the rear oxygen sensor of two sensor were fault-installing by changing the sensor of other a car it could not detect of oxygen quantity. Finally, it found the phenomenon of abruptly decreasing vehicle speed when braking a car. Therefore, the system including with emission control has to drastically manage by maximizing condition to role decreasing the emission gas.

A Study on the Interpretation of the Traditional Private Garden Pavilion in Honam Province from the Perspective of Confucian Frame of Self-Cultivation by Its Location and Arrangement (유가적 수양론으로 본 호남지방 별서형 정자 입지와 배치의 의미해석에 관한 연구)

  • Kim, Jin-Su;Kim, Yun-Sang
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.28 no.4
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    • pp.49-63
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    • 2010
  • This study focused on the method of separation - space organization- as one of the architectural methods that is considered by the view point of its location and arrangement of 'Byoul-seo' private garden pavilion in Honam province. As a result, the followings are conclusions; By this study, they can be found in the concept of '居敬窮理', '格物致知'. It is presumed that nature union is archived through the 'yan-sang(玩賞)' which is ultimate state of knowledge that is based on the premises of opened significance in emancipation from narrow view point in the past and cognition of value. Therefore, it is needed to know the unworldly point of space perception about the pavilion territory, furthermore to have conceptual method to distinct pavilion from the world. there are two methods in the distinct concept in this pavilion research: unworldly separation and meditative separation. It is concluded that the specific consideration of the method of separation that is applied in the Byoul-seo pavilion. And it is accomplished by the contemplation of separation method in approach and entrance of direct influence area. they could be 1) cross the hill, 2) cross over a brook, 3) lifted up in the mount, 4) penetrate the forest, and 5) narrow entrance, which could be part of location.

The study of improving the performance of lower direction finding ability due to the interfered phase difference of circular array Antennas (원형배열안테나의 위상간섭에 의한 방향탐지 성능저하 개선연구)

  • Chung, Jae-Woo;Kim, Young-Kil
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2010.05a
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    • pp.535-539
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    • 2010
  • This paper include to study DoA(Direction of Arrival) for radio collection and monitoring system. The direction finding calculated by applying the CVDF (Correlation Vector Direction Finding) algorithm for the five circular dipole antenna over V / UHF band. To improve the accuracy of direction finding by applying CVDF algorithm needs to obtain ideal phase difference each antennas. However, a circular array antenna phase difference pattern may be distorted on a specific frequency band or to particular direction. The effect of installing each array antennas circularly and the effect of the interference of center pole (located in the center of a circular array antenna mount) may make the distortion of phase pattern. If you use an active antenna instead of passive antenna to obtain good sensitivity, you would measure the more distortion. This paper propose how to change combination of antennas to measure the phase in real-time and how to use antenna beam patterns for minimizing the degradation phenomena at applying simple CVDF algorithm and increasing the direction finding capability.

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Design of an Anamorphic Aspherical Prism Lens for the Head Mount Display (HMD용 회전 비대칭 비구면 프리즘 렌즈 설계)

  • Park, Seung-Hwan;Lee, Dong-Hee
    • Journal of Korean Ophthalmic Optics Society
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    • v.13 no.4
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    • pp.83-88
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    • 2008
  • Purpose: To design an anamorphic aspherical prism lens for the HMD optical system. Methods: First, we get the initial data, needed in design, which are distances between each surface etc., by analyzing user's demended specifications and by drawing geometrically the shape of prism lens by using CAD. Based on these data and using 'ode V' which is an optical design software, we could progress the optimization in which we treat the coefficients of the anamorphic aspherical surface as the principal variables. To reduce the cost in DTM manufacturing, we would optimize the optical system with the transmitting surface, existed in the direction of video device among 3 surfaces of the prism lens, remaining as a plane. Results: we could design one anamorphic aspherical prism lens which has the finite ray aberration of 15 ${\mu}m$, the distortion of 0.5%, and the MTF value of 0.3 over at 36 lp/mm for the video device of 12 mm ${\times}$ 9 mm size. Conclusions: We designed a prism lens used for HMD. This prism lens has the optical capacities of 15 ${\mu}m$ finite ray aberration and 0.5% distortion for the video device of 12 mm ${\times}$ 9 mm size, and become the optical system having the MTF value of 0.3 over at 36 lp/mm.

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Proteomic Analyses of Chinese Cabbage(Brassica campestris L. pekinensis) Affected by High Temperature Stresses in Highland Cultivation During Summer in Korea (Proteomics를 이용한 고랭지 배추의 고온장해 해석)

  • Shin, Pyung-Gyun;Hong, Sung-Chang;Chang, An-Cheol;Kim, Sang-Hyo;Lee, Ki-Sang
    • Journal of Life Science
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    • v.17 no.12
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    • pp.1649-1653
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    • 2007
  • High temperature stresses have caused growth inhibition and delayed heading in highland cultivation Chinese cabbage during summer in Korea. We have studied high temperature stress responses in the terms of changes of inorganic components and proteins by proteomic analyses. Insufficiencies of nitrogen and phosphorus have affected growth rate and calcium deficiency has caused blunted heading. Proteins extracted from Brassica seedling grown at the altitude of 600m and 900m in the Mount Jilun were extracted and analysed by 2-dimentional polyacrylamide gel electrophoresis. Profiles of protein expression was then analyzed by 2-dimentional gel analyses. Protein spots showing different expression level were picked using the spot handling workstation and subjected to MALDI-TOF MS. Total 48 protein spots were analyzed by MALDI-TOF MS and 30 proteins spots out of 48 were identified by peptide mass fingerprinting analyses. Fourteen proteins were up-regulated in extracts from the altitude of 900m and they were identified as oxygen-evolving proteins, rubisco activase and ATPase etc. Sixteen proteins were up-regulated in extracts from the altitude of 600m and they were identified as glutathione S-transferase(1, 28kD cold induced- and 24 kD auxin-binding proteins) and salt-stress induced protein etc. These stress-induced proteins were related to the mediated protective mechanism against oxidative damage during various stresses. The results indicated that physiological phenomenon in response to high temperature stresses might be resulted by complex and multiple array of responses with drought, heat, oxidative, salt, and cold by high temperature.

The Effect of Medication in the Patients with Bee-Sting (약물투여가 벌자상환자에게 미치는 효과)

  • Cho, Byung-Jun;Moon, Soo-Jae;Kim, Seon-Rye
    • The Journal of the Korea Contents Association
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    • v.15 no.1
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    • pp.350-356
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    • 2015
  • The purpose of this study is effect factors of Pre-Hospital Medication in the Patients with Bee-Sting. This study is to identify the necessity of using epinephrine in prehospital stage from the perspective of early management of anaphylaxis following a bee sting. Methods: Patients suffering from a bee sting who used the 119 rescue between 2013 from 119 center data were included. Age, sex, month of injury, time factors, vital signs, medication, signs, distance factors, presence of cardiac arrest, AVPU triage by EMT were extracted. The severity of bee sting injury was divided into mild, moderate, and severe according to the presenting symptoms and signs. Results: The severe patients treated by using oxygen, IV, ECG, Medication. Anaphylaxis is a serious allergic reaction of rapid onset that may lead to death. One of Anaphylaxis patients lived by using epinephrine. Most patients collapsed at the scene of mount. Bee sting injuries occurred primarily during summer to the farmer. Nine patients collapsed at the scene. Bee sting injuries occurred primarily from June to October. Conclusion: The primary treatment for anaphylaxis is epinephrine. Epinephrine in pre-hospital stage is factors of essential for patients. Also epinephrine will permit to be equipped in the EMT-P and use of epinephrine by law.

A study on alluvial deposits of tributaries of Yungsan river, near Damyang. (담양지역 영산강 지류 하천 퇴적층의 특성에 대한 연구)

  • Kim, Jong Yeon;Hong, Se Sun
    • Journal of The Geomorphological Association of Korea
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    • v.20 no.4
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    • pp.51-70
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    • 2013
  • The characteristics of deposits formed by the Daejon-cheon and Soobuk-cheon, dissecting the mountains such as Byungpung Mt. and Samin Mt. in western part of Damyang county, Jeonmam province. Results from field survey and bore hole logging by KIGAM are used in interpreting depositional environment, in this study. By the result of deposits near of the channels Daejon-cheon and Soobuk-cheon, and main channel of Youngsan River, the depth of sediment layers in this area is 4~7m, far thinner than formerly estimated. Weathered material of local rocks forms the base of the sedimentary layers. It can be assumed that the location channel of the Youngsan river has been stable ever since the start of the sedimentary events. Sediment particles of tributaries are angular than those of Youngsan River. Particles are larger and sorting is poor. It is interpreted as mount flash flood deposits. Main sources of sediments at the valley bottom or deposition dominated area are the terrace deposits or slope deposits over the gentle foot-slope or front of surrounding mountains. Some particles show polygonal cracking on the surface originated from the strong chemical weathering, while most of these has high angularity. It means various geomorphic processes operate to produce and transport the particles in this area.Isolated hills within the sedimentary plains are made with weathered materials of local bedrock. In the case of foot-slope of the hills, thin sedimentary layers are found. So it can be concluded that surface features of deposition zone of the Daejon-cheon and Soobuk-cheon is formed by the filling of lower part of the valley and its feature partly controlled by the relief of the weathering front.

A Study on the Flight Safety Test of Drones for the Establishment of Toy Drone Safety Standards (완구용 드론 안전기준 재정을 위한 드론의 비행 안전성 테스트 연구)

  • Jin, Jung-Hoi;Kim, Gyou-Beom;Jin, Sae-Young
    • Journal of Convergence for Information Technology
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    • v.9 no.12
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    • pp.141-146
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    • 2019
  • Economic analysis predicts that the drone market will grow, and the growth of the toy and hobby drone market is expected to gradually expand. Drone expectations are rising due to the net economic function of drone market growth, but accidents due to improper management and operations are also increasing. The difference in toy drone performance is incomparably small compared to industrial drone performance, but the ordinary buyer can not know whether the difference can cause an accident during use. The toy drones used in this study were obtained from KC and CE certification, and 20 kinds of drones were used. The flight time ranged from a minimum of 3 minutes to a maximum of 12 minutes, and the control distance ranged from a minimum of 20m to a maximum of 380m. Therefore, it is necessary to secure product safety through sampling inspection of the radio wave output of toy drones, and it is also necessary to mount an algorithm that automatically lowers the altitude or hover when exceeding the limit flight distance. For future research, we will build data to establish toy drone safety standards through a altitude testing and impact testing of toy drone.