• 대한전자공학회:학술대회논문집
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• 대한전자공학회 1998년도 하계종합학술대회논문집
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• pp.23-25
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• 1998

Many of the currently used PN code acquisition algorithms detect the phase of the incoming PN signal on the basis of ML estimation principle and utilize statistics grounded in taking inner products. By showing that any set of 2n-1 PN sequences arising in SSRG or MSRG (those typically used in IS'95 implementations) configuration constitutes a linearly independent set and that the number of candidate PN sequences has to equal the dimension of the span of the candidate PN sequences, we prove that the lowerbounding computational complexity involved in any PN code acquistion, utilizing (only) inner product computations at each stage of acquisition, corresponds precisely to those, such as double dwell acquistion circuitries, currently used.

• 대한한방내과학회지
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• 제1권1호
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• pp.64-69
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• 1976

Kami Eui In Tang is being used as a treatment oral medicament on the appendicitis and peritonitis without surgical operation resulting good effect. The component of the medicament are as follow : Coix ma yuen, Paeonia moutan, Patrinia scabiosifolia, Rheum, Coreanum, Cory-dalis nakaii, Trichosanthes kirilowii, Lonicera japonica, Prunus persica, Tarxacum officinale, Author tried to make clear the ant inflammatory effect on rat paw which has become edema formation by carrageenin and detect the active ingredients by thin layer chromatography method. The material was extracted with three kind of solvent; distilled water, 50% ethyl alcohol, and ether which were immersed in material for 48 hours and concentrated to 10ml by evaporation of solvents. The result was as follow: 1. The anti-inflammatory effect of the sample material on the. edema formed at the rat paw was obviously clear. 2. Many spots of active ingredients were detected on the silica gel G plate spotted by ether extracted material than water one. 3. The good developing solvent was the mixture of $CHCl_3$ ; Acetone : Diethylamine (5:4:1).

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2008년도 하계종합학술대회
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• pp.217-218
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• 2008

A new diversity technique is proposed for multi-user single-input single-output (SISO) orthogonal frequency division multiplexing (OFDM) uplink system. Each user transmit a symbol using frequency band of all users that are active in the uplink. In addition, a receiver detect each signal using maximum likelihood (ML) method. Simulation results show that the proposed technique improves a diversity order without an additional bandwidth and a loss of data rate.

• Journal of Microbiology and Biotechnology
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• 제17권4호
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• pp.681-684
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• 2007

An immunoassay method was developed to quantitatively detect phosphinothricin-N-acetyltransferase (PAT) encoded by the Bialaphos resistance (bar) gene in genetically modified (GM) pepper. The histidine-tagged PAT was overexpressed in Escherichia coli M15 (pQE3l-bar) and efficiently purified by $Ni^{2+}$ affinity chromatography. A developed sandwich enzyme-linked immunosorbent assay (S-ELISA) method (detection limit: $0.01{\mu}g/ml$) was 100-fold more sensitive than a competitive indirect ELISA (CI-ELISA) method or Western blot analysis in detecting the recombinant PAT. In real sample tests, PAT in genetically modified herbicide-tolerant (GMHT) peppers was successfully quantified [$4.9{\pm}0.4{\mu}g/g$ of sample (n=6)] by the S-ELISA method. The S-ELISA method developed here could be applied to other GMHT crops and vegetables producing PAT.

• Journal of electromagnetic engineering and science
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• 제6권3호
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• pp.176-181
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• 2006

Signal distortion due to the path loss, shadow, and multi-path fading is very serious in radio channel. In this paper, we propose CDMA-OFDM cooperative communication system based on DFP(Decode and Forward Protocol) to overcome these phenomena using spread spectrum technique, orthogonal sub-carrier, and the space diversity. We simulated proposed system under Rayleigh flat fading channel environment. A variety of simulation results reveal the cooperation can provide performance gain of up to 12 dB over direct communications in ideal inter-user case at BER of $10^{-3}$. And we can also confirm variation of diversity effect as channel environment changes.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.150.3-151
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• 2003

To analyze the genome of Cucumber mosaic virus(CMV) in pepper, we developed a new extraction method for double-stranded RNA(dsRNA). To isolate the dsRNA, 0.1g of pepper leaves homogenized with 1ml of 5${\times}$EXB extraction buffer[0.5M glycin, 0.5M NaCl, 5mM EDTA(pH9.0/NaOH), 10％ Sodium N-lauryl salcosinate(NLS), 10％ Sodium dodecylsulfate(SDS)] and purified with the 1/4 volume of phenol： chloroform： isoamylalcohol(25：24：1). dsRNAs from the aqueous phase was precipitated with isopropanol. This procedure was able to detect a minimal amount of dsRNA from CMV infected plant tissue and to distinguish different CMV satellite RNAs by polyacrylamide gel electrophoresis(PAGE). Moreover, this method can be applied CMV infected in pepper or Rice dwarf virus (RDV) infected rice.

• 대한수의학회지
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• 제16권1호
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• pp.11-25
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• 1976

Studies on pathogenicities and developmental stages of Nosema apis (Zander, 1909) were carried out through artificial infection to Nosema free honey bees with various levels of spores isolated from local honey bee colony. The results obtained were summarized as follows: 1. The clinical symptoms were observed as dysentery, enteritis of mid-gut (enlargement and decoloration), crawling posture and shortening of the longevity of worker bees in the rearing honey bee colony inoculated with the spores. 2. Number of spores harvested from laboratory rearing honey bees were progresively increased to 4 weeks after inoculation. The regression equations and coefficients of correlations to various spore levels were as follows in each treatment colony. Colony 1. ($$1,000{\times}10^4spores/ml$$) $$y_{c1}=471{\times}10^{4}x+454{\times}10^4(r=0.65^*$$) Colony 2. ($$500{\times}10^4spores/ml$$) $$y_{c2}=340{\times}10^{4}x+207.8{\times}10^4(r=0.99^{**}$$) Colony 3. ($$100{\times}10^4spores/ml$$) $$y_{c3}=150{\times}10^{4}x+84.2{\times}10^4(r=0.99^{**}$$) Colony 4. ($$10{\times}10^4spores/ml$$) $$y_{c4}=13.8{\times}10^{4}x+13{\times}10^4(r=0.98^{**}$$) 3. Average longevity of worker bees artificially infected with Nosema apis was shortened as 21.7~43.8% compare to the control. (p<.05, p<.01) 4. The spores which were isolated from honey bee colony infected with Nosema disease were ovoid or spherical form, and measured, as a rule, from $4.7{\mu}m$ to $6.1{\mu}m$ (mean $5.3{\mu}m$) in length and from $2.4{\mu}m$ to $3.2{\mu}m$ (mean $2.9{\mu}m$) in width. 5. In the mid-gut of honey bees, the spore was progresively germinated and became trophozoite stage. The trophozoites were grown to meronts and their binary fission were begun. The divided two sporoblasts were developed to the spores which had elastic membrane. The new spores were shed in excreta of honey bees 10~15 day after inoculation at $25{\pm}2$ centigrade. 6. The ultrastructure of spore membrane consisted of three layers, such as, outer, middle and inner layer. The sporoplasm consisting lamellar structure occupied only anterior part of the spore and was often extended to posterior direction where definite vacuoles and a polar filament was able to detect.

• 한국의학물리학회지:의학물리
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• 제17권4호
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• pp.238-245
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• 2006

CT 검사에서 조영제 혈관외유출을 예방하기 위해 EDA 시스템을 개발하여 가토를 대상으로 실험적으로 혈관외유출의 검출 효율을 평가하였다. 7마리의 가토 IV 정맥 부위에 EDA 시스템의 스트레인게이지를 부착하여 조영제를 주입하면서 혈관외유출 검출 효율을 평가하였다. 조영제의 혈관외유출은 7건으로 혈관외유출 용량 $14{\sim}23ml$ (평균 18.86 ml), 혈관외유출 발생시간 평균 11.29초, 평균압력 109.71 psi, EDA 시스템에서 스트레인게이지의 분해능 평균 512.18, 진양성 7건, 진음성 19건, 위양성 2건이었고, 민감도 100%, 특이도 90%로 나타났다. 개발된 EDA 시스템을 가토에 실험적으로 적용하여 소량의 혈관외유출 조영제를 검출하는 효율이 있어 혈관외유출을 예방하는 효과가 있다.

• 한국미생물·생명공학회지
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• 제26권3호
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• pp.238-243
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• 1998

느타리버섯의 세균성갈반병균인 P. tolaasii(PT)를 신속 간편하게 검출할 수 있는 효소면역측정법 (ELISA)을 개발하였다. 특이항체를 생산하기 위하여 PT($5{\times}10^7$ cfu)를 Freund's adjuvant와 함께 토끼에 수차례 면역하였으며, 가장 높은 역가를 나타낸 항혈청을 이용하여 비경합 ELISA 및 경합 ELISA를 각각 확립하였다. 표준곡선으로부터 PT의 그 검출한계는 각각 $2{\times}10^2$cfu/ml 및 $3{\times}10^2$cfu/ml가량으로 나타났다. 교차반응은 비경합 ELISA의 경우 P. agarici, P. reactans 및 비병원성 Pseudomonas속 균주와 1/$10^3$이하의 매우 미약한 반응을 보였으나 P. solanacearum, Erwinia chrysanthemi, Streptococcus mutans, Xanthomonas citri 및 곰팡이인 Fusarium oxysporum등 다른 균주와의 반응성이 거의 없었으며, 경합 ELISA의 경우 PT이외에는 같은 속 균주에 대하여도 반응성이 거의 없었다. ELISA를 이용하여 버섯으로부터 분리된 18균주에 대하여 PT의 여부를 조사하였을 때 병원성과 white line 반응성을 동시에 나타내었던 11균주에 대하여만 명확한 양성으로 나타나, 본 연구에서 확립한 ELISA는 간편하고 정확한 PT검출법 임이 확인되었다.

• 대한화학회지
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• 제30권4호
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• pp.369-376
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• 1986

모세관 기체-액체프로마토그래피(GLC)에 의해 인도네시아산 Costus speciosus중의 diosgenin을 정량하기 위해 가수분해 및 추출, 아세틸화 반응 및 GLC의 측정 조건등을 검토하였다. 건조된 diosgenin분말 1.20g을 3N HCI과 xylene의 혼합용액으로 95∼100${\circ}C$에서 4시간 환류시킨 다음 xylene층을 분리하였다. Xylene을 증발시켜 날려보내고 20:80의 acetic anhydride-pyridine을 가하고 30분간 환류시켜 아세틸화시킨 후 diethyl ether로 추출하였다. 무수 $Na_2SO_4$로 탈수시키고 ether를 날려 보낸 다음 찌끼를 n-hexane 5.00ml에 녹여 GLC로 분석하였다. SE-30 25m ${\times}$ 0. 33mm의 모세관을 사용하였고 컬럼온도는 180${\circ}$에서 270${\circ}C$까지 10${\circ}C$/min의 속도로 온도를 상승시켰다. 운반기체 $N_2$의 유속은 2ml/min이었고 FID로 검출하였다. Diosgenin의 분석결과는 0.281%이었고, 상대표준편차는 1.8%로서 재현성이 좋았다.