• Microbiology and Biotechnology Letters
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• v.15 no.6
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• pp.396-401
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• 1987

Protein productivities of a cloned gene ($\beta$-galactosidase) and the cultural performances of two recombinant Escherichia coli strains, which use different host-vector systems, were studied. E. coli JM109/pTBG10 strain which carries Tac promoter had higher protein productivity than E. coli MH3000 (pRKc1857)/pASI(lacZ) strain which carries pL promoter. Induction of protein syn-thesis was optimum at the initial-and mid-logarithmic growth phases for both strains. Oxygen demand was observed to be very high during the cloned gene expression, and could be alleviated to some extent through pH control. The ratio of specific growth rates of plasmid-harboring to plasmidfree cell, $\mu$+ /$\mu$-, of the high productivity strain was observed to be lower than that of the low productivity one. Plasmid stability was analyzed for 20-30 generations, and it was found that the traction of plasmid-harboring cells dropped to l0% level in about 25 generations for both strains when the cloned gene expression was induced.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.10B
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• pp.891-899
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• 2006

Generally, a geographical area consists of many regions called cells in wireless networks. Each mobile host(MH) in a cell communicates with a base station(BS) located in the center of the cell. The BSs are connected to each other by a static wired network. Since users are expected to move around during communication sessions, one of the most important QoS factors is related to the handoff. But, the handoff could fail due to unavailability of sufficient bandwidth in the next cell. As the individual cell size gradually shrinks to accommodate increasing the number of MHs, the probability of successful handoffs can be dropped. In this paper, we suppose a bandwidth reservation method to guarantee MHs against connection failure in case of frequent handoffs. This method predicts the mobility based on the geographical information and assigns the bandwidth reservation in proportion to the speed of MHs' motion. As a result, we can expect more exact moving path of MH and to reduce the waste of bandwidth.

• Journal of Korean Society on Water Environment
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• v.36 no.4
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• pp.300-313
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• 2020

The Bayesian approach can be used to estimate hydrologic model parameters from the prior expert knowledge about the parameter values and the observed data. The purpose of this study was to compare the performance of the two Bayesian methods, the Metropolis-Hastings (MH) algorithm and the Generalized Likelihood Uncertainty Estimation (GLUE) method. These two methods were applied to the TANK model, a hydrological model comprising 13 parameters, to examine the uncertainty of the parameters of the model. The TANK model comprises a combination of multiple reservoir-type virtual vessels with orifice-type outlets and implements a common major hydrological process using the runoff calculations that convert the rainfall to the flow. As a result of the application to the Nam River A watershed, the two Bayesian methods yielded similar flow simulation results even though the parameter estimates obtained by the two methods were of somewhat different values. Both methods ensure the model's prediction accuracy even when the observed flow data available for parameter estimation is limited. However, the prediction accuracy of the model using the MH algorithm yielded slightly better results than that of the GLUE method. The flow duration curve calculated using the limited observed flow data showed that the marginal reliability is secured from the perspective of practical application.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.13 no.1
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• pp.33-39
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• 2002

Background and Objectives : The vocal fold nodules which were made by excessive contact or vibration of the vocal folds were classified to the soft nodule and the hard nodule in according to the hardness or the duration of nodule. Sometimes laryngologist saw the nodule to be located in different level. Authors thought that each nodule to be located in different level might have the different causes. Therefore we studied to know the relationship between the voicing technique and each vocal fold nodule to be located in different level. Materials and Methods : One-hundred forty nine patients who had the vocal fold nodule were evaluated. Sites and shapes of the vocal fold nodules were investigated using videostroboscopy. Videokymography was also used to scan the center of the vocal fold nodules during phonation and classified to several types. Same procedures were done on normal subject while he simulated the various types of voicing. And we compared the findings between both of them. Three different types of lesion can be distinguished. These are ML group that lesions were located from mid to low, MH group that lesions were located from mid to upper and HL group that lesions were located from lower to upper of the vocal folds. Results : The VKG findings of ML group and situation simulating with hard glottal attack and vocal fry were similar. MH group had a similar VKG findings with situation simulating with whispering or high pitch voicing. HL group had a similar VKG findings with situation simulating with loud voicing. Conclusions : Authors thought that each vocal fold nodule, which had different shapes and located in different level, related with the different types of voicing.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.150-156
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• 2000

Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. The 30 fatty acids which were identified or characterized are classified. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Cluster 3 and 4-a strains were containing Q-8, these strains are accommodated in the Proteobacteria gamma and beta subdivision. The chemotaxonomic profiles of the cluster 4-a strains showed good agreement with those of the genus Burkholderia. Cluster 3 was characterized by the presence of branched-chain fatty acids, iso-C15:0, iso-C17:1, and iso-C17:0 as the major components. These chemotaxonomy suggested the close relationship of the isolates with Xathomonas/Sterotrophomonas group. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Sterotrophomonas, but were not included in these genera. These strains were even further away from core Xanthomonas, and clearly were seen to branch outside the cluster formed by the Sterotrophomonas maltophilia. MH256 and MA828 16S rDNA sequence was different enough to put new genus on a separate branch. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.11 no.2
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• pp.165-171
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• 2017

We suggested the novel actuator mechanism to apply to wearable assistive system for the improvement of quality of life of the elderly or the people with disability using it. Characteristics of metal-hydride (MH) actuator is investigated in the novel actuating concept. The hydrogen equilibrium pressure increases when hydrogen is desorbed by heating a SMH alloys, whereas by cooling that alloys, the hydrogen equilibrium pressure decreases and hydrogen is absorbed. However, there are too long times in heat transfer mechanism to apply the assistive and rehabilitative device. In this study, 3 different SMH module were designed and characteristics of heat transfer in each SMH module were investigated based on the heat simulation.

• Journal of the Korean Electrochemical Society
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• v.9 no.2
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• pp.95-99
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• 2006

Electrochemical properties were studied for a single particle of active material of hydrogen storage alloy $(MmNi_{3.55}Co_{0.75}Mn_{0.4}Al_{0.3})$ and nickel hydroxides $(NiOH)_2$ for the secondary Nickel Metal Hydride (Ni-MH) batteries using the microelectrode, which was manipulated to make electrical contact with an active material particle for cyclic voltammograms (CV) and potential-step experiments. As a result of CV test, it was found that three kinds of hydrogen oxidation peaks at -0.9, -0.75 and -0.65 V and hydrogen evolution peak at -0.98 V for hydrogen storage alloy were separately observed and two kinds of peaks of proton oxidation/reduction at 0.45 and 0.32 V and oxygen evolution reaction (OER) at 0.6 V for nickel hydroxides were also more clearly observed. Furthermore hydrogen diffusion coefficient within a single particle was also found to vary the order between $10^{-9}\;and\;10^{-10}cm^2/s$ over the course of hydrogenation and dehydrogenation process for potential-step experiments.

• The KIPS Transactions:PartC
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• v.13C no.3 s.106
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• pp.345-352
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• 2006

In the integrated wired/wireless network which consists of the Internet and moving networks, multiple wireless links are used to connect a fixed host(FH) in the Internet to a mobile host(MH) in the moving network. For use in such an environment, we propose a scheme to overcome the TCP performance degradation due to the packet losses over the wireless links without losing the end-to-end TCP semantics. The proposed scheme in each mobile router(MR) allows to obtain the information regarding packet losses over the upstream wireless links based on the received packet sequence number and the ACK number. This information is delivered to the upstream router, which enables the upstream access router(AR) or MR to quickly retransmit the lost packets. The proposed scheme has the feature to quickly recover the packet losses incurred over the upstream wireless links and the performance of the proposed scheme is evaluated through simulation. It is shown that the significant performance gain can be obtained using the proposed scheme compared with the snoop mechanism which maintains end-to-end TCP semantics and does not require any additional features at the source and/or destination nodes.

• The Transactions of the Korean Institute of Power Electronics
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• v.7 no.1
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• pp.30-40
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• 2002

This paper studies the electronic ballast development for 250w MH lamps. We have improved the input power factor using a PFC IC. To provide the rating voltage required In the lamps, we have used the buck type dc-dc converter By this method, the stress of switching devices in inverter can be reduced. The inverter is the Full-Bridge type. To eliminate the acoustic resonance phenomena of MH lamps, we have added the high frequency sinewave voltage to the low frequency square-wave voltage to the lamp. We hove developed the igniter circuit using the L, C devices. We could control dimming of the lamp by varying the output voltage of the buck converter. The time of illuminating lamps and luminous intensity could be adjusted by season and time band. The buck converter output voltage can be controlled and the no load and over current situation were Protected by the development of the microprocessor Program.

• Journal of Microbiology
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• v.38 no.4
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• pp.265-269
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• 2000

Iron increased the susceptibilities of clinical isolates Pseudomonas aeruginosa to quinolones. In the presence of iron, increased susceptibilities to ofloxacin were observed in twenty-six out of thirty isolates and with no change in four isolates. In the case of norfloxacin, iran increased susceptibilities of twelve isolates but did not render any change in eighteen isolates. In the case of ciprofloxacin, iron decreased the MICs (Minimal Inhibitory Concentration) of twenty isolates, increased the MIC of one isolate, and did net change the MICs of nine isolates. To find out how iron increased susceptibility to ofloxacin, bacterial cells were grown in Muller Hinton (MH) media and succinate minimal media (SMM) to induce iran acquisition systems and the intracellular ofloxacin concentrations were assayed in the presence of iron. The addition of iron to the media decreased the MICs of cells whether they were grown in MH or SMM. Siderophores, carbonyl cyanide m-chlorophenylhydrazone (an inhibiter of proton motive force), and ouabain (an inhibitor of ATPase) did not decrease the effect of iron. Results suggested that the increase in the intracellular ofloxacin concentration by iron is accomplished not by decreasing the efflux but by increasing the of ofloxacin permeability.