• 제목/요약/키워드: MG-63 cell

검색결과 331건 처리시간 0.026초

Bone Nodule Formation of MG63 Cells is Increased by the Interplay of Signaling Pathways Cultured on Vitamin $D_3$-Entrapped Calcium Phosphate Films

  • Choi, Yong-Seok;Hong, Yoon-Jung;Hur, Jung;Kim, Mee-Young;Jung, Jae-Young;Lee, Woo-Kul;Jeong, Sun-Joo
    • Animal cells and systems
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    • 제13권4호
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    • pp.363-370
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    • 2009
  • Since vitamin $D_3$ is an important regulator of osteoblastic differentiation, a presently-established vitamin $D_3$-entrapped calcium phosphate film (VCPF) was evaluated for hard tissue engineering. The entrapped vitamin $D_3$ more rapidly induced bone nodule formation. To characterize the cellular events leading to regulations including faster differentiation, signal transduction pathways were investigated in osteoblastic MG63 cells at a molecular level. Major signaling pathways for MG63 cell proliferation including phosphatidylinositol-3-kinase, extracellular signal-regulated kinase, c-Jun N-terminal kinase and focal adhesion kinase pathways were markedly down-regulated when cells were cultured on calcium phosphate film (CPF) and VCPF. This agreed with our earlier observations of the immediate delay in proliferation of MG63 cells upon culture on CPF and VCPF. On the other hand, the p38 mitogen-activated protein kinase (p38 MAPK) and protein kinase A (PKA) pathways were significantly up-regulated on both CPF and VCPF. CPF alone could simulate differential behaviors of MG63 cells even in the absence of osteogenic stimulation and entrapment of vitamin $D_3$ within CPF further amplified the signal pathways, resulting in continued promotion of MG63 cell differentiation. Interplay of p38 MAPK and PKA signaling pathways likely is a significant event for the promotion of differentiation and mineralization of MG63 cells.

St. John's Wort (Hypericum perforatum) stimulates human osteoblastic MG-63 cell proliferation and attenuates trabecular bone loss induced by ovariectomy

  • You, Mi-kyoung;Kim, Du-Woon;Jeong, Kyu-Shik;Bang, Mi-Ae;Kim, Hwan-Seon;Rhuy, Jin;Kim, Hyeon-A
    • Nutrition Research and Practice
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    • 제9권5호
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    • pp.459-465
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    • 2015
  • BACKGROUND/OBJECFTIVES: The effect of St. John's Wort extract (SJW) on MG-63 cell proliferation and trabecular bone loss induced by ovariectomy was examined. MATERIALS/METHODS: Proliferation, expression of estrogen receptor (ER) ${\alpha}$ and ER ${\beta}$, and gene expressions of osteoprotegerin (OPG), osteocalcin (OC) and alkaline phosphatase (ALP) were examined in MG-63 cells treated with or without SJW. Ovariectomized rats were treated with SJW at the dose of 100 or 200 mg/kg/day, ${\beta}$-estradiol-3-benzoate (E2), or vehicle only (OVX-C), and sham operated rats were treated with vehicle only (Sham-C). Serum ALP and C-telopeptide (CTX), and femoral trabecular bone loss were examined. RESULTS: SJW increased MG-63 cell proliferation and expression of ER ${\alpha}$ and ER ${\beta}$, and positive effect was shown on gene expressions of ALP, OC and OPG. SJW also showed estrogen like effect on bone associated with slowing down in trabecular bone loss. Histopathology by H&E showed rats treated with SJW displayed denser structure in metaphyseal region of distal femur compared with rats in OVX-C. SJW was shown to reduce serum CTX in OVX rats. CONCLUSION: The present study provides new insight in preventing estrogen deficiency induced bone loss of SJW and possibility for its application in bone health supplement.

니코틴이MG63 조골세포주의 오스테오칼신과오스테오프로 테제린의 생성 및 mRNA 발현에 미치는 영향 (Effects of nicotine on the formation of osteocalcin and osteoprotegerin and synthesis of its mRNA in MG63 osteoblast-like cell)

  • 양준호;박수병
    • 대한치과교정학회지
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    • 제34권6호
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    • pp.514-525
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    • 2004
  • 흡연이 골형성 세포의 기능을 억제한다는 것은 이미 잘 알려져 있으나 대다수의 연구가 주로 역학조사에 기초하여 이루어져 왔다. 본 연구의 목적은 흠연과 골형성 세포의 상관관계를 실험적으로 평가하기 위한 것이다. 본 연구를 위해 인간 골육종 세포인 MG63 조골제포주를 이용하였으며, 니코틴이 조골세포의 증식, 염기성 인산분해효소의 활성, 오스테오칼신곽 오스테오프로테제린의 생성 그리고 mRNA발현에 미치는 영향을 관찰하였다 MG63조골세포를 니코틴 농도가 각각 $1.0{\mu}M$,\;1.0mM, 2.5mM, 5.0mM, 7.5mM, 그리고 10.0mM,이 함유된 배지에서 1일, 2일, 3일 그리고 6일 동안 배양 실험을 하여 다음과 같은 결과를 얻었다. MG63 조골세포의 증식이 저농도의 니코틴에서는 일시적으로 활성화되었지만 5.0mM 이상의 고농도에서는 억제되었다. 염기성 인산분해효소의 활성은 니코틴 농도가 증가함에 따라 감소하였다. 오스테오칼신 생성양은, 배양 1일 후, 5.0mM 이하의 농도에서 증가하였으나 7.5mM 이상의 고농도에서는 감소하였다. MG63 조골세포를 3일 배양한 경우, 오스테오칼신 생성양은 1.0mM의 저농도에서도 감소하였다 니코틴이 오스테오칼신 단백질 생성과 오스테오칼신 mRNA생성에 미치는 영향은 1일과 3일에서 다소 차이가 있었다. 오스테오프로테제린의 생성양은 모든 실험군에서 니코틴을 함유하지 않은 대조군보다 감소하였다. 하지만 mRNA 수치는 단백질 생성과 상반되게 7.5mM (3일), 5.0mM (6일) 이상의 고농도에서 증가하였다.

GENE-EXPRESSION PROFILING OF TITANIUM-CELL INTERACTION

  • Kim, Chang-Su;Hwang, Jung-Won;Ryu, Jae-Jun;Shin, Sang-Wan;Sohn, Sung-Hwa;Kim, Ki-Nam;Kim, Meyoung-Kon
    • 대한치과보철학회지
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    • 제43권3호
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    • pp.393-408
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    • 2005
  • Statement of problem. In the process of bone formation, titanium (Ti) surface roughness is an important factor modulating osteoblastic function. Purpose. This study was carried out to determine the effect of different Ti surface on biologic responses of a human osteoblast-like cell line (MG63). Materials and methods. MG63 cells were cultured on S (smooth), SLA (sandblasted largegrit & acid etching), HA (hydroxyapatite) Ti. The morphology and attachment of the cells were examined by SEM. The cDNAs prepared from total RNAs of MG63 were hybridized to a human cDNA microarray (1,152 elements). Results. The appearances of the surfaces observed with SEM were different in the three types of dental substrates. The surface of SLA and HA were shown to be rougher than S. MG63 cells cultured on SLA and HA were cell-matrix interaction. In the expression of genes involved in osseointegration, upregulated genes were bone morphogenetic protein, Villin, Integrin, Insulin-like growth factors in different surfaces. Downregulated genes were fibroblast growth factor receptor 4, Bcl 2-related protein, collagen, CD4 in different surfaces. Conclusion. The attachment and expression of key osteogenic regulatory genes were enhanced by surface roughness of the dental materials.

사불상 및 와피티 사슴의 혈액 및 혈청화학치 (Hematological and Serum Chemical Values in Pere David′s Deer and Wapiti)

  • 신남식;권수완;한덕환;이흥식
    • 한국임상수의학회지
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    • 제11권1호
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    • pp.471-478
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    • 1994
  • The hematologic values and serum chemistry were examined healthy male 12 Pere David's Deer and 8 Wapiti at the Farmland Zoological Garden. In wapiti, the blood values collected from horn and jugular vein were compared. The results were as follows: 1. There were not any significant differences in hematologic values and serum chemistry of blood col. looted from horn and jugular vein in wapiti. 2. In Pere David's Deer, Mean$\pm$SD Values of White Blood Cell(WBC) count were 4673.50$\pm$1893.42/${mu}ell$, of Neutrophils 65.42$\pm$12.7%, of Lymphocytes 31.50$\pm$8.68%, of Monocytes 0.25$\pm$0.43%, of Eosinophils 0.17$\pm$0.37%. of Basophils, of Red Blood Cell(RBC) 9.93$\pm$1.38$\times$10/${mu}ell$, of PCV 54.25$\pm$6.25%, of Hb 16.89$\pm$2.43g/㎗, of Tot protein 7.63$\pm$0.6g/㎗, of Cholesterol 87.25$\pm$10.76mg/㎗, of Glucose 109.36$\pm$43.7mg/㎗, of SGOT 85.17$\pm$33IU/$\ell$, of SGPT 102.92$\pm$31.49IU/$\ell$, of BUN 21.00$\pm$5.18mg/㎗, of Creatinine 2.1$\pm$0.43mg/㎗, of ALKP 156$\pm$32.72IU/$\ell$, of Ca 11.28$\pm$1.04mg/㎗, of Na 127.92$\pm$10.04mmo1/$\ell$, of NH3 102.83$\pm$42.25mmo1/$\ell$, of Cl 105.91$\pm$3.45mmo1/$\ell$, respectively. 3. In wapiti, Mean$\pm$SD Values of White Blood Cell(WBC) count were 3450.00$\pm$1040.73/${mu}ell$, of Neutrophils 53.88$\pm$7.42%, of Lymphocytes 36.00$\pm$5.72%, of Monocytes 1.13$\pm$1.05%, of Eosinophils 1.38$\pm$1.93%, of Basophils 7.63$\pm$4.15%, of Red Blood Cell 10.09$\pm$1.3$\times$10/${mu}ell$, of PCV 46.88$\pm$3.33%, of H 15.09$\pm$1.48g/㎗, of Total protein 7.15$\pm$0.73g/㎗, of Cholesterol 78.63$\pm$12.27mg/㎗, of Glucose 75.63$\pm$33.22mg/㎗ , of SGOT 79.25$\pm$18.43IU/$\ell$, of SGPT 36.50$\pm$19.20IU/$\ell$, of BUN 29.63$\pm$4.15mg/㎗, of Creatinine 1.35$\pm$0.21mg/㎗ , of ALKP 283.50$\pm$88.13IU/$\ell$, of Ca 9.78$\pm$0.43mg/㎗ , of Na 120.00$\pm$10.02mmol/$\ell$, of NH3 261.50$\pm$ 103.46mmol/$\ell$, of Cl 92.00$\pm$9.19mmol/$\ell$, respectively.

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Effect of Titanium Coating on Cell Adhesion and Extracellular Matrix Formation in Human Osteoblast-like MG-63 Cells

  • Lee, Jae-Bum;Seo, Sang-Hui;Kim, Yu-Ri;Shin, Sang-Wan;Kim, Meyoung-Kon;Ryu, Jae-Jun
    • Molecular & Cellular Toxicology
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    • 제4권3호
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    • pp.192-198
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    • 2008
  • A variety of titanium (Ti) and its alloys are used in the clinical procedures of bone regeneration for periodontal and dental implant therapies. This study was performed to determine the effect of different surface dental implant materials on biologic responses of a MG-63 human osteoblast-like cell line. MG-63 cells were cultured on Ti coated with hydroxyapatite (HA), calcium metaphosphate (CMP), anodized (A), which compared with non-coated Ti (control). The appearances of surface of dental implant materials and the morphology of these cells were assessed by scanning electron microscopy (SEM). The gene expression profiles of MG-63 cells cultured on Ti were examined by human cDNA microarray (1,152 elements). The expression of several genes was up- and down-regulated by different surfaces of dental implant materials. Interesting, the genes correlated with cellular adhesion and extra cellular matrix (ECM) formation were enhanced, in accordance surface morphology of the dental implant materials used.

유산균을 이용한 대두 발효 추출물이 면역계 활성에 미치는 영향 (Effects of soybean extracts fermented with Lactic acid bacteria on immune system activity)

  • 박병두;김혜자
    • 대한예방한의학회지
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    • 제16권3호
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    • pp.139-153
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    • 2012
  • Objectives : NK cells are spontaneously cytotoxic lymphocytes. These are not only important parts in the first line of defence against bacterial and viral infections of outside, but they may also play a critical role in chronic viral diseases. NK cells kill their targets spontaneously, without the need for prior sensitization and class I MHC restriction by the regulation of cytolytic functions and secretion of a variety of cytokines, such as interleukin-12(IL-12), MCP-1, IL-6, TNF-${\alpha}$, IFN-${\gamma}$. In addition, macrophage and NK cells cooperate through the production of cell mediates. These cooperation and modulation are one of major factors to prevent for evading immune surveillance of cancer. Hence, it could be assumed that if any candidate to enhance activities of macrophage and NK cell, it is considered as a potentially useful agents against cancer. Methods : In our study, to investigate effect of fermented soybean extracts by Lactic acid bacteria (SFE, soybean fermented extracts) work on intestinal immune cell to maintain general immune modulating and anti-cancer activity. We analyzed NK cytotoxicity assay and gene expressions of cytokine related with macrophage and NK cell activity. Results : In vitro experiment, SFE was verified as safety material for cell toxicicty to tumor cell strain without any toxicity of tumor growth inhibition and various cell strain. Effects of macrophage activity stimulating directly by SFE measured induced cytokine. The studies showed that IL-12 production by stimulation of SFE depended on concentration from 0.16mg/mL to 0.63mg/mL with non toxicity to cell, and it was the best activity at 0.63mg/mL. Besides, the effective concentration of SFE producing TNF-${\alpha}$ is similar to IL-12, but it was the best activity at 1.25mg/mL. The level of MCP-1, IL-6 and IFN-${\gamma}$ depended on concentration from 0.16mg/mL to 10mg/mL, IFN-${\gamma}$ showed the best activity at the effective concentration of 0.63mg/mL. With the result of NK cell activity measurement, the spleen cell of mouse injected SFE had 1.5 times higher killing effect than non injected cell. Conclusions : The result of this studies is that Soybean fermetated extracts(SFE) has possibility to immune aided material for the function not only inhibition of microbial infection to macrophage but also activity of adaption immune and cellular immune system.

가미장신탕(加味長身湯)이 뼈성장 관련 인자에 미치는 영향에 대한 실험적 연구 (Experimental Studys of GMJST on Bone Growth Factors;Proliferation of Osteoblast and Supression of Osteoclast)

  • 한덕희;안정조;조현경;유호룡;김윤식;설인찬
    • 동의생리병리학회지
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    • 제22권4호
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    • pp.802-809
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    • 2008
  • Gamijangsing-tang (GMJST) has been used for treatment of bone formation in traditional korean medicine. The purpose of this study is to examine effects of GMJST on bone metabolism. The effects on the osteoblasts were determined by measuring (1) cell proliferation, (2) alkaline phosphatase (ALP) activity, (3) osteoprotegerin (OPG) secretion. (4) The morphologic changes of cells were observed by light microscopy and electron microscopy. Mineralization of calcium was determined by quantitative alizarin red-S assay and mineralization of phosphate was observed by von kossa staining. The morphologic changes of mineralization on the cells were observed by transmission electron microscopy (TEM). The effects on the osteoclast were investigated by tartrate-resistant acid phosphatase (TRAP) staining. Following results were obtained: Celluar activity of osteoblastic cells (MG-63) was significantly increased in 10-5 of dilution of GMJST. ALP and OPG activity of osteoblastic cells were increased in GMJST than normal MG-63 cell. Mineralization of osteoblastic cells were increased in GMJST than normal MG-63 cell. The activity of osteoclast cells (RAW 264.7) was significantly decreased in GMJST than normal MG-63 cell. From the results, GMJST stimulated the proliferation and mineralization of bone-forming osteoblast and inhibited by bone- lysis osteoclast.

Biological Effects of Different Thin Layer Hydroxyapatite Coatings on Anodized Titanium

  • Sohn, Sung-Hwa;Jun, Hye-Kyoung;Kim, Chang-Su;Kim, Ki-Nam;Ryu, Yeon-Mi;Lee, Seung-Ho;Kim, Yu-Ri;Seo, Sang-Hui;Kim, Hye-Won;Shin, Sang-Wan;Ryu, Jae-Jun;Kim, Meyoung-Kon
    • Molecular & Cellular Toxicology
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    • 제1권4호
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    • pp.237-247
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    • 2005
  • Several features of the implant surface, such as roughness, topography, and composition play a relevant role in implant integration with bone. This study was conducted in order to determine the effects of various thin layer hydroxyapatite (HA) coatings on anodized Ti surfaces on the biological responses of a human osteoblast-like cell line (MG63). MG63 cells were cultured on A (100 nm HA coating on anodized surface), B (500-700 nm HA coating on anodized surface), C ($1{\mu}m$ HA coating on anodized surface), and control (non HA coating on anodized surface) Ti. The morphology of these cells was assessed by SEM. The cDNAs prepared from the total RNAs of the MG63 were hybridized into a human cDNA microarray (1,152 elements). The appearances of the surfaces observed by SEM were different on each of the four dental substrate types. MG63 cells cultured on A, C and control exhibited cell-matrix interactions. It was B surface showing cell-cell interaction. In the expression of several genes were up-, and down-regulated on the different surfaces. The attachment and expression of key osteogenic regulatory genes were enhanced by the surface morphology of the dental materials used.

신장 상피세포주 A498을 이용한 대황(Rheum undulatum L.)추출물의 세포독성 (Cytotoxicity of Crude Extracts of Rheum uudulatum L. with Human Kidney Epithelial Cell A498)

  • 나명석;진종언;조남철
    • 한국식품영양학회지
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    • 제13권5호
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    • pp.460-464
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    • 2000
  • 본 연구에서는 전통적으로 담석증, 신장치료 등의 한약제로 많이 사용하는 대왕을 여러 용매를 사용하여 얻은 대왕추출물 분액에 대한 세포독성을 여부를 MTT 정량법, NR 정량법, SRB 정량법을 이용하여 조사하였다. 1. 추출 용매 methylene chloride, ethyl acetate, butanol, water로부터 얻은 대왕추출물 모두 처리농도에 따라 세포에 미치는 영향이 증가하였다. 2. Butanol을 용매로 사용하여 얻은 대왕추출물 분액이 다른 3가지 용매로부터 얻은 대왕추출물보다 세포에 미치는 영향이 크게 나타났고 water를 용매로 사용하여 얻은 추출물이 A498 세포주에 미치는 영향이 가장 낮게 나타났다. 3. Butanol을 추출 용매로 하여 얻은 대왕추출물이 A498 세포주에 미치는 영향이 가장 컸는데 그 추출물에 대한 MTT$_{50}$, NR$_{50}$, SRB$_{50}$값은 각각 0.63mg/ml, 0.65mg/ml, 0.68mg/ml이었고, 가장 영향이 적은 water의 경우 MTT$_{50}$, NR$_{50}$, SRB$_{50}$값은 각각 0.84mg/ml, 0.82mg/ml, 0.80mg/ml이었다. 4. 정량방법 간의 대왕추출물에 대한 반응은 MTT 정량법이 가장 민감하게 나타났다.

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