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A Study on the Constituents of the Pollen of Pinus densiflora Siebol et Zuccarini and Pinus rigida Miller -Proximate Composition, Mineral, Heavy Metal, Vitamin and Free Sugar Contents- (적송화분과 리기다송화분의 성분 조성에 관한 연구 -일반성분, 무기질, 중금속, 비타민, 유리당의 함량-)

  • 김혜자
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.2
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    • pp.201-206
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    • 1992
  • Pinus pollen is used as the chief ingredient for Song-wha-dashik (Korea's traditional pattern-pressed candy). It has been the food stuff indispensable especially on the occasion of performing ancestral rites and holding a banquet since Shilla and Koryo Dynasty. In this paper, therefore, a new examination is made of the nutritive ingredients of the very Pinus pollen. Analysis has been made of the proximate composition, minerals, heavy metals, vitamins and free sugars of the Pollen of both Pinus densinora Siebol et Zuccarini and Pinus rigida Miller. In the proximate composition, Pinus rigida Miller is high except in free sugar ; crude ash (4.0%), crude protein (15.0%), crude fat (2.5%), crude fiber (17.5%). A lot of minerals have been found in both the samples. They contain Mg, Cl, Ca, Fe, Mn, Na, Zn, etc. In heavy metals, both the samples contain the same quantity of Cd(0.3 ppm) and the contents of Pb, Cu, and As are lower than the maximal limit permitted for food stuff. The contents of vitamin B$_1$, B$_2$, vitamin C are a little higher in Pinus rigida Miller : in vitamin E, on the contrary, Pinus densiflora Siebol et Zucarini (1.6 mg) doubles Pinus rigida Miller (0.8 mg). In free sugars, the results is as follows : 6.6%/6.9% in sucrose : 0.2% in fructose : 2.1% /2.9% in glucose respectively.

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Antimicrobial Activities of Methanol Extracts Obtained from Several Ferns (양치식물류의 메탄올 추출물에 항균활성 분석)

  • Shin, So-Lim;Lee, Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.23 no.5
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    • pp.436-444
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    • 2010
  • Methanol extracts of the aerial and rhizome parts obtained from ten species of ferns has been screened for antimicrobial activities against Bacillus subtilis, Escherichia coli, Listeria monocytogenes, Propionibacterium acnes, Streptococcus mutans and Streptococcus sobrinus. Antimicrobial activities were carried out using broth microdilution method and paper disc diffusion assay and the extracts which showed clear zones more than 15mm in concentration of 2 mg/disc were tested for its antimicrobial activities at the $0.125{\sim}2\;mg{\cdot}mL^{-1}$ concentration of each extract for three days. The non-sterilized crude methanol extracts of Osmunda cinnamomea var. fokiensis rhizome showed the highest antimicrobial activities on B. subtilis(39%), E. coli (33%) and L. monocytogenes(58%) at the concentration of $2\;mg{\cdot}mL^{-1}$ after 72 hours. In P. acnes, frond extract of O. cinnamomea var. fokiensis showed most vigorous antimicrobial activities in the all extracts but it showed weak activity(clear zone diameters below 15 mm). All extracts has the antimicrobial activities on Streptococcus, but they exhibited weak activity. At the concentration of $2\;mg{\cdot}mL^{-1}$, only Osmunda japonica rhizome extracts showed 28 and 39% of antimicrobial activities on S. mutans and S. sobrinus after 72 hours and the other extracts showed below 10% of antimicrobial activities on S. mutans and S. sobrinus.

Preparation of Activated Carbon from Wastepaper and Adsorption of Endocrine Disrupting Chemicals

  • Okayama, Takayuki;Matsushita, Kiyofumi;Sasuzuki, Hiroma;Shimada, Masahiro
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2006.06b
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    • pp.279-284
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    • 2006
  • Activated carbon is proposed as a new application of wastepaper recycling other than the paper-making. Waste kraft bag is considered to be a suitable raw material for activated carbon because of its low ash content. Small pellets of wastepaper squeezed out from the continuous kneader were carbonized in a nitrogen atmosphere and activated using carbon dioxide. The BET specific surface areas of activated carbon prepared from waste kraft bag was $1,285m^{2}/g$, which is higher than commercially available activated carbons. The activated carbon prepared from wastepaper has a well-developed porous structure, particularly in mesopore and macropore ranges. As a result, activated carbon with iodine adsorption capacity of 1,400 mg/g was obtained from waste kraft bag. In this paper, adsorption amount of Bisphenol A (BPA) was determined to investigate adsorbability of activated carbon from waste kraft bag. Adsorption measurements were on solutions ranging from $0.1{mu}g/L\;to\;100mg/L$. The activated carbon from waste kraft bag gave higher BPA adsorbabilities over a wide range, compared with commercially available activated carbons.

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Antioxidative and Physiological Activities of Fractions from Pleurospermum kamtschaticumin Extracts (누룩치(Pleurospermum kamtschaticumin) 추출물의 항산화 효과 및 생리활성)

  • Kim, Mi-Seon;Kim, Kyoung-Hee;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.10
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    • pp.1338-1345
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    • 2012
  • This study investigated the antioxidant activities, whitening effects, and antimicrobial activities of fractions from Pleurospermum kamtschaticumin extract. Total polyphenolic contents of fractions from Pleurospermum kamtschaticumin extract were 116.44~382.73 mg/g GAE (gallic acid equivalent), with the highest value in the ethyl acetate fraction. Fractions of Pleurospermum kamtschaticumin showed the highest DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity ($IC_{50}$=0.04 mg/mL) and ABTS radical scavenging activity (98.86% at 0.5 mg/mL), which was similar to ascorbic acid. Further, reducing power and FRAP activity were significantly high in the ethyl acetate and n-butanol fractions. The ethyl acetate and n-butanol fractions showed significantly high SOD-like activities (1 mg/mL, 86.93%, and 78.23%, respectively) compared to other fractions. Tyrosinase inhibition activities of the n-butanol fraction and 80% ethanol extract were 43.52% and 41.64%, respectively. Antimicrobial activity against Escherichia coli was only observed in the chloroform fraction (16.7 mm in 0.5 mg/disc). These results suggest that fractions from Pleurospermum kamtschaticumin extract show comparatively high antioxidant activity and thus could be used as a food additive or cosmeceutical ingredient.

STUDIES ON THE VARIATION IN CHEMICAL CONSTITUENTS OF THE SEA MUSSEL, MYTILUS EDULIS (진주담치 Mytilus edulis의 성분에 관한 연구)

  • CHOI Woo-Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.1
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    • pp.38-44
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    • 1970
  • This paper deals with the proximate composition of the cultured sea mussel (Mytilus edulis) which are distributed along the whole coastline of Korea, particularly abundant in the southern coast Studies on seasonal variation in chemical constituents of the mussel at Northern Bay of Choongmu were carried out from March to December 1968 and the results obtained are as follows: 1. Moisture content in mussel meat was $78.5\%$ on the average; the maximum amounted to $81.3\%$ during May-June, while the minimum was $77.8\%$ in September. 2. Crude protein was in the range of $10.9-13.7\%$; the maximum was In September-October, the minimum appeared in March, and the average value was $12.8\%$. 3. Lipids on the average was $2.5\%$ and there was no markable difference .between the high and low contents. 4. Total sugar was $5\%$ during September-October in its highest, while there appeared minor contents during winter season. 5. Crude ash on the average was $1.5\%$ the and maximum was in November-December. As for minerals in the ash, $963mg\%$ of phosphate, $82mg\%$ of calcium, and $188mg\%$ of iron were found respectively. 6. pH was in the range of 6.02-6.55, but it generally declined to acidity in the summer season. 7. In amino acid contents, there found 16 kinds; $710mg\%$ of glutamic acid, $696mg\%$ of aspartic acid, $383mg\%$ of Iysine, $225.4mg\%$ of valine, $225.1mg\%$ of proline, etc. 8. The amounts of Protein, lipids and total sugar are tend to increase from August to October, particularly in September.

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Antimicrobial Effect of the Submerged Culture of Sparassis crispa in Soybean Curd Whey (순물을 이용한 꽃송이 버섯 균사체 배양액의 항균활성 평가)

  • Lee, Eun Ji;Kim, Ji-Eun;Park, Min-Ju;Park, Dong-Cheol;Lee, Sam-Pin
    • Food Science and Preservation
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    • v.20 no.1
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    • pp.111-120
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    • 2013
  • Sprassis crispa was cultivated using soybean curd whey, and its antimicrobial activities were examined against those of eight microorganisms that were foodborne pathogens or food-poisoning bacteria. The culture broth of soybean curd whey was superior in mycelium content (17.76 g/L) to that of the defined culture broth, and the ${\beta}$-glucan content was about 10.64 percent (w/w). The antimicrobial activities of the culture broth were confirmed against those of B. cereus, St. aureus, L. monocytogenes and S. typhimurium using the paper disk method. The antimicrobial activity was also maintained after the heat treatment and alcalase treatment. The filtrate with less than 3 kDa M.W. also showed the antimicrobial activity against four strains: B. cereus, St. aureus, L. monocytogenes and S. typhimurium. The minimum inhibitory concentration (MIC) was about 1.26 mg/mL in the B. cereus and 12.6 mg/mL in the St. aureus and L. monocytogenes. The S. typhimurium showed a MIC of 62.8 mg/mL. Thus, the culture of Sparassis crispa using soybean curd whey provides a thermally stable antimicrobial agent that can be used as a natural preservative in the biofood industry.

Production Technology of Titanium by Kroll Process (Kroll법에 의한 타이타늄의 제조기술)

  • Sohn, Ho-Sang
    • Resources Recycling
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    • v.29 no.4
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    • pp.3-14
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    • 2020
  • Titanium sponge is industrially produced by the Kroll process. In order to understand the importance of the emerging smelting and recycling process, it is necessary to review the conventional production process of titanium. Therefore this paper provides a general overview of the conventional titanium manufacturing system mainly by the Kroll process. The Kroll process can be divided into four sub-processes as follows: (1) Chlorination of raw TiO2 with coke, by the fluidized bed chlorination or molten salt chlorination (2) Magnesium reduction of TiCl4 and vacuum distillation of MgCl2 and Mg by reverse U-type or I-type with reduction-distillation integrated retorts (3) Electrolysis process of MgCl2 by monopolar cells or multipolar cells to electrolyze into chlorine gas and Mg. (4) Crushing and melting process in which sponge titanium is crushed and then melted in a vacuum arc furnace or an electron beam furnace Although the apparatus and procedures have improved over the past 80 years, the Kroll process is the costly and time-consuming batch operation for the reduction of TiCl4 and the separation of MgCl2.

Antimicrobial Activity against Food Hazardous Microorganisms and Antimutagenicity against Salmonella serotype Typhimurium TA100 of an Ethanol Extract from Sanguisorba officinalis L. (지유 에탄올 추출물의 식품 위해성 세균에 대한 항균 활성 및 Salmonella serotype Typhimurium TA100에 대한 항돌연변이 활성 효과)

  • Kim, Se-Ryoung;Won, Ji-Hye;Kim, Mee-Ra
    • Korean journal of food and cookery science
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    • v.27 no.4
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    • pp.17-26
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    • 2011
  • This study was performed to analyze the antibacterial activity against food hazardous microorganisms and antimutagenic effects of Sanguisorba officinalis L. ethanol extracts on Salmonella Typhimurium TA100. The antibacterial activity was evaluated by paper disc diffusion assay, minimum inhibition concentration (MIC), and optical density of the culture with the ethanol extract for 24 hr. Antibacterial activity was tested with seven microorganisms including Escherichia coli, Escherichia coli O157:H7, Pseudomonas aeruginosa, Salmonella Typhimurium, Listeria monocytogenes, Bacillus cereus, and Staphylococcus aureus. The paper disc diffusion assay showed distinct clear inhibition zones around the discs treated with the extract for five microorganisms, except Escherichia coli and Escherichia coli O157:H7. MIC values were 0.625-2.5 mg/mL for these five strains that showed clear zones. The time-kill assay was consistent with the results from the paper disc diffusion assay and MIC test. Additionally, antimutagenicity of the extract was determined using the Ames test. The ethanol extract at 5 mg/plate inhibited 72.42% and 89.85% of mutagenicity induced by 4-nitroquinoline 1-oxide and sodium azide, respectively. These results demonstrate that the ethanol extract from S. officinalis L. has remarkable antibacterial activity and antimutagenicity.

A Study on the Trace Metal Contents in Food by Neutron Activation Analysis (방사화 분석법에 의한 식품 중 미량금속(Mg, Zn, Mn, Mo and Se)에 관한 연구)

  • 이숙경
    • Journal of Food Hygiene and Safety
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    • v.12 no.4
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    • pp.328-332
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    • 1997
  • In order to investigate the trace metals in Korean foods, the contents of Magnesium, Zinc, Manganese, Molybdenum and Selenium are studied in this paper. As show in the Table 1; a total of 250 samples of 25 species were analyzed by neutron activation analysis. The results obtained were as follows; 1. The overall ranges and mean (mg/100 g) were; Mg, 12.212∼151.346(55.164); Zn, 0.045∼38.180 (2.473); Mn, 0.003∼0.796 (0.225);Mo, ND∼0.035 (0.007); Se, ND∼0.069 (0.016). 2. The levels of all metals except Mo in shell fishes were high and the level of Mo in spices was higher than that in other foods. 3. The levels of Zn and Mo in oyster were higher than another spcies.

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Clinical In Vivo Bio Assay of Glucose in Human Skin by a Tattoo Film Carbon Nano Tube Sensor

  • Ly, Suw Young;Lee, Chang Hyun
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.3
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    • pp.595-601
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    • 2017
  • In vivo assay of glucose detection was described using a skin tattoo film electrode (STF), and the probe was made from carbon nano tube paste modification film paper. Here in the square-wave stripping anodic working range obtained of $20-100mgL^{-1}$ within an accumulation time of 0 seconds only in sea water electrolyte solutions of pH 7.0. The relative standard deviations of 50 mg glucose that were observed of 0.14 % (n=12), respectively, using optimum stripping accumulation of 30 sec, the low detection limit (S/N) was pegged at 15.8 mg/L. The developed results can be applied to the detect of in vivo skin sensing in real time. Which confirms the results are usable for in vitro or vivo diagnostic clinical analysis.