• 한방안이비인후피부과학회지
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• 제26권1호
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• pp.35-49
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• 2013

Objectives : We aimed to determine therapeutic effects of probiotics-fermented Magnolia denudata(MD) in the allergic rhinitis model mice. Methods : Polyphenol production, DPPH radical scavenging activity and NO inhibition of fermented MD by different bacterial strains were evaluated to select the one that is most suitable for fermentation. Thirty C57BL/6 mice were divided randomly into 5 groups as follows: normal group, ovalbumin(OVA)-treated plus water fed(CON group), OVA-treated plus unfermented MD fed(UMD group), OVA-treated plus fermented autoclaved MD fed(A-FMD group) and OVA-treated plus fermented unautoclaved MD fed(FMD group). After 9 weeks, we observed changes in the blood cell count, OVA-specific IgE level, nasal rubbing, nasal mucosal tissue and body weight. Results : Extract of MD fermented by Bifidobacterium breve(BB) for 48 hours showed the highest anti-oxidant activity and anti-inflammatory activity out of all the other bacterial strains. The number of eosinophil count in A-FMD, FMD group and platelet count in FMD group showed statistically significant decrease(p<0.05). OVA-specific IgE level decreased in all 3 experimental groups, significantly in UMD and A-FMD group. Nasal symptoms were attenuated in all 3 experimental groups, statistically significant in A-FMD and FMD group (p<0.05). Histologically, infiltration of eosinophils into the nasal mucosa decreased in all 3 experimental groups, especially marked decrease in FMD group. Conclusions : According to the above results, it is considered that probiotics-fermented Magnolia denudata has inhibitory effects on the allergic rhinitis animal models.

• 한국가금학회지
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• 제34권1호
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• pp.57-76
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• 2007

Marek's disease (MD) is caused by a ubiquitous, lymphotropic alphaherpesvirus, MD virus (MDV). MD has been a major concern in the poultry industry due to the emergence of increasingly virulent strains over the last few decades that were isolated in the face of comprehensive vaccination. MD is characterized by a variety of clinical signs, amongst them neurological symptoms, chronic wasting, and most notably the development of multiple lymphomas that manifest as solid tumors in the viscera and musculature. Much work has been devoted to study MD-induced oncogenesis and genes involved in this process. Among the many genes encoded by MDV, a number have recently been shown to affect the development of tumors in chickens, one protein directly causing transformation of cells (Meq) and another being involved in maintaining transformed cells (vTR). Other MDV gene products modulate and are involved in early lytic in vivo replication, thereby increasing the chance of transformation occurring. In this review, specific genes encoded by MDV that are involved in the initiation and/or maintenance of transformation were briefly summarized, and limits of current vaccination and new control strategies against MD, particularly how modem molecular biological methods may be used to improve strategies to combat the disease in the future, were discussed.

• 한국해양바이오학회지
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• 제3권1호
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• pp.54-60
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• 2008

Compared to other North African countries, Tunisia has reached a significant level of fish consumption. The only relevant historical dimension of aquaculture in Tunisia are traditional lagoon management (80,000 ha of lagoon and coastal lakes) and culture of shellfish. Semi-intensive and intensive cultures are relatively new concepts in Tunisia and only recently also the public sector is involved. The Tunisian fishing industry has expanded over the last 20 years and annual catches at present are more than four times those registered in mid-fifties. Production of the year 2007 reached 105 thousand tons against 111 thousand tons during the same period of 2006 thus recording a fall of 5%. Unfavorable weather conditions mainly during the last quarter year had the effect to reduce the number of days out at sea. Exports reached 24.3 thousand tons for one value 240.5 MD against respectively 22.2 thousand tons and 234.1 thus recording MD at the end of the past year a rise of 9% in volume and from 3% in value. Commercial value such as shellfish - consequence of one regression of the production - with in parallel raises blue fish exports. The imports were stabilized in volume of 39.1 thousand tons and increased from 6% in value with respectively 67.4 MD in 2007 against 63.7 MD at the end of 2006. The importation in larger quantities of intended fish to the fattening of tuna in floating cages explains partly this rise. Nevertheless, the pay of balance import/export of produced fishing remains positive with a surplus of 173.1 MD against 170.4 MD in 2006.

• Experimental and Molecular Medicine
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• 제50권11호
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• pp.7.1-7.12
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• 2018

Recent findings from The Cancer Genome Atlas project have provided a comprehensive map of genomic alterations that occur in hepatocellular carcinoma (HCC), including unexpected mutations in apolipoprotein B (APOB). We aimed to determine the clinical significance of this non-oncogenetic mutation in HCC. An Apob gene signature was derived from genes that differed between control mice and mice treated with siRNA specific for Apob (1.5-fold difference; P < 0.005). Human gene expression data were collected from four independent HCC cohorts (n = 941). A prediction model was constructed using Bayesian compound covariate prediction, and the robustness of the APOB gene signature was validated in HCC cohorts. The correlation of the APOB signature with previously validated gene signatures was performed, and network analysis was conducted using ingenuity pathway analysis. APOB inactivation was associated with poor prognosis when the APOB gene signature was applied in all human HCC cohorts. Poor prognosis with APOB inactivation was consistently observed through cross-validation with previously reported gene signatures (NCIP A, HS, high-recurrence SNUR, and high RS subtypes). Knowledge-based gene network analysis using genes that differed between low-APOB and high-APOB groups in all four cohorts revealed that low-APOB activity was associated with upregulation of oncogenic and metastatic regulators, such as HGF, MTIF, ERBB2, FOXM1, and CD44, and inhibition of tumor suppressors, such as TP53 and PTEN. In conclusion, APOB inactivation is associated with poor outcome in patients with HCC, and APOB may play a role in regulating multiple genes involved in HCC development.

• 수산해양교육연구
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• 제27권5호
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• pp.1334-1342
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• 2015

본 연구에서는 연어 오일의 미세캡슐화를 위해 다양한 피복물질을 사용해 보았다. 피복물질의 종류가 미세캡슐화 수율에 큰 영향을 미쳤으며, 피복물질로 사용한 MD/SC/WPI 혼합 처리구에서 수율이 82.55%로 가장 우수한 성능을 보였다. SEM 분석 결과 입자의 분포가 균일하고 섬유 표면의 질감이 매끄럽게 나타났으며, 저장성 실험결과 pH, 산가, 과산화물가에서 안정한 수치를 나타내었기에 제품화 시 유통기한 30일까지는 실온저장에서 안전할 것으로 사료된다. 또한 MD/SC/WPI를 이용하여 분말화 한 연어 오일은 유화안정성이 높고, 제품의 취급이 쉽게 이용 될 수 있을 것이다. 이에 본 연구에서의 캡슐화 방법이 연어 오일과 유사한 특성을 지닌 물질의 캡슐화에 응용 될 수 있을 것이다.

• 생물환경조절학회지
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• 제25권4호
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• pp.240-248
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• 2016

식물 호르몬의 일종인 ABA의 처리가 사과 과피의 착색에 미치는 영향을 알아보기 위하여, 착색이 시작되는 시기의 'Hongro' 사과에 abscisic acid (ABA)와 ABA의 생합성 저해제인 fluridone (FD)을 처리한 뒤 과피의 색변화를 관찰하였다. 실험 결과, ABA와 FD 처리에 의해 'Hongro' 사과의 착색이 큰 영향을 받지 않았다. 과피의 붉은 색을 나타내는 hunter a값이 처리 7일 후까지 처리간에 차이가 없었다. 실제 과피의 안토시아닌 함량은 과피의 hunter a값의 변화와 같은 경향을 보이며 증가하였으며, ABA나 FD의 처리가 과피의 안토시아닌 축적에 영향을 주지 않았다. ABA의 처리 직후 과피의 내생 ABA함량이 급격히 증가하였고 처리 종료 시까지 높게 유지되었다. FD 처리구의 과피 내 ABA함량은 처리 6시간 후부터 대조구보다 낮아지다가, 처리 4일 후까지 지속적으로 감소하였다. ABA의 처리에 의해서 과피의 MdNCED2 (ABA 생합성 유전자)의 발현량이 증가하였고, MdACO1 (ethlyene 생합성 유전자) 및 안토시아닌 생합성 유전자 중 MdCHS와 MdDFR의 발현량이 증가하였다. 하지만 MdUFGT의 발현량은 ABA 처리에 의해서 변화가 없었다.

• Nutrition Research and Practice
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• 제7권4호
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• pp.281-286
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• 2013

We examined the effect of parental folate deficiency on the folate content, global DNA methylation, folate receptor-alpha (FR${\alpha}$), insulin-like-growth factor-2 (IGF-2) and -1 receptor (IGF-1R) in the liver and plasma homocysteine in the postnatal rat. Male and female rats were randomly fed a folic acid-deficient (paternal folate-deficient, PD and maternal folate-deficient, MD), or folic acid-supplemented diet (paternal folate-supplemented, PS and maternal-folate-supplemented, MS) for four weeks. They were mated and grouped accordingly: $PS{\times}MS$, $PS{\times}MD$, $PD{\times}MS$, and $PD{\times}MD$. Pups were killed on day 21 of lactation. The hepatic folate content was markedly reduced in the $PD{\times}MD$ and $PS{\times}MD$ and $PD{\times}MS$ as compared with the $PS{\times}MS$ group. The hepatic global DNA methylation was decreased in the $PD{\times}MS$ and $PS{\times}MD$ groups as much as in the $PD{\times}MD$ group, and all the three groups were significantly lower as compared to the $PS{\times}MS$ group. There were no significant differences in the hepatic FR${\alpha}$, IGF-2 and IGF-1R expressions among the groups. Positive correlations were found between the hepatic folate content and global DNA methylation and protein expressions of FR${\alpha}$, IGF-2 and IGF-1R, whereas an inverse correlation was found between hepatic folate content and plasma homocysteine level in the 3-week-old rat pup. The results of this study show that both paternal and maternal folate deficiency at mating can influence the folate content and global DNA methylation in the postnatal rat liver.

• 한국산학기술학회논문지
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• 제20권1호
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• pp.35-40
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• 2019

멀티캐스트를 이용하는 실시간 응용에서는 끊임없이 서비스를 제공하는 것이 중요하다. IP 멀티캐스트와 응용계층 멀티캐스트(ALM)를 이용하는 하이브리드 멀티캐스트에서는 혼잡 등 네트워크 상황에 적응하기 위하여 전송경로의 재구성이 발생한다. 이로 인하여 종단 간 지연시간이 증가하고 실시간 서비스의 품질이 저하되는 문제가 발생한다. 본 논문에서는 이런 문제를 다계층 전송경로를 구성하여 해결하고자 한다. 제안방법에서는 하이브리드 멀티캐스트 구성을 위해서 제어서버와 각 멀티캐스트 도메인(MD)에 존재하는 응용계층 오버레이 호스트(Application Layer Overlay Host)를 둔다. 제어서버는 MD에 가입한 ALOH들로 부터 제어정보를 받아 홉 수를 기반으로 그룹을 생성하고, 모든 ALOH들에게 전송한다. 각 MD의 ALOH는 타 MD의 ALOH에게 오버레이로 패킷을 전송하는 역할과 다계층 전송경로를 구성하는 역할을 수행한다. 다계층 전송경로는 제어서버로 부터 전송받은 제어정보와 이웃한 ALOH 간 지연시간을 이용하여 우선순위별로 구성된다. 이렇게 완성된 다계층 전송경로 중 혼잡이 발생하거나 ALOH의 부재 시에는 가장 우선순위가 높은 전송경로를 선택하여 종단 간 지연시간을 줄이도록 한다. 시뮬레이션 결과는 제안방법이 혼잡상태에서 종단 간 지연시간을 평균 289ms 이하로 줄일 수 있었음을 보여준다.

• 한국전산구조공학회논문집
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• 제27권3호
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• pp.137-145
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• 2014

본 논문에서는 브리징 스케일 분해를 기반으로 멀티스케일 문제에 대한 설계민감도 해석법을 개발하였다. 나노 기술의 급속한 발전으로 인해 나노 수준의 해석의 필요성이 지속적으로 증가하고 있다. 최근 분자동역학과 연속체역학의 연성문제에서 많은 해석 방법들이 개발되었다. 본 논문에서는 연성시스템 해석을 위해 브리징 스케일 기법을 사용한다. 전체 영역의 분자동역학 시스템의 해석은 많은 양의 계산 비용이 들기때문에 분자동역학과 연속체 시뮬레이션의 연성시스템을 선호한다. 분자동역학과 연속체 수준 사이의 정보 교환은 분자동역학과 연속체의 경계에서 일어난다. 브리징 스케일 법에서 일반화된 랑지벵 방정식은 축소된 영역의 분자동역학 시스템 해석을 위하여 요구되고, 시간이력 커널을 사용하여 구한 GLE 힘은 분자동역학 시스템에서 경계에 있는 원자들에 작용한다. 그러므로 분자동역학과 연속체 수준의 시뮬레이션을 분리해서 해석할 수 있으며 계산 과정을 가속시킬 수 있다. 연성문제의 시뮬레이션 이후에는 설계의 최적화를 위해 설계민감도 해석의 필요성이 자연스럽게 나타나며 전체 시스템의 성능은 나노 스케일의 효과를 고려해서 최적화된다. 설계구배 기반 최적화에서 설계민감도가 요구되지만 유한차분법으로 구한 민감도는 문제가 대형화될 때 계산비용의 제한때문에 비실용적이나 해석적 설계민감도는 효율적인 강점을 갖는다. 본 연구에서는 연성된 분자동역학-연속체 멀티스케일 문제에서 해석적 설계민감도를 유도하여 정확성과 향후 최적설계로의 활용 가능성을 확인하였다.

• 상하수도학회지
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• 제34권4호
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• pp.259-266
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• 2020

In this study, a pilot-scale (3 ㎥/day) membrane distillation (MD) process was operated to treat digestate produced from anaerobic digestion of livestock wastewater. In order to evaluate the performance and energy cost of MD process, it was compared with the pilot scale (10 ㎥/day) reverse osmosis (RO) process, expected competitive process, under same feed condition. As results, MD process shows stable permeate flux (average 10.1 L/㎡/hr) until 150 hours, whereas permeate flux of RO process was decreased from 5.3 to 1.5 L/㎡/hr within 24 hours. In the case of removal of COD, TN, and TP, MD process shows a high removal rate (98.7, 93.7, and 99% respectively) stably until 150 hours. However, in the case of RO process, removal rate was decreased from 91.6 to 69.5% in COD and from 93.7 to 76.0% in TP during 100 hours of operation. Removal rate of TN in RO process was fluctuated in the range of 34.5-62.9% (average 44.6%) during the operation. As a result of energy cost analysis, MD process using waste heat for heating the feed shows 18% lower cost compare with RO process. Thus, overall efficiency of the MD process is higher then that of the RO process in terms of permeate flux, removal rate of salts, and operating cost (in the case of using waste heat) in treating the anaerobic digestate of livestock wastewater.