• Journal of Life Science
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• v.2 no.1
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• pp.26-34
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• 1992

유전자 재조합 균주의 발효에 있어서 플라스미드의 안정성과 발효공정의 최적화에 대해 개략적으로 서술하였다. 클론된 DNA의 발현은 플라스미드의 안정성을 크게 저해하며, 저하된 플라스미드의 안정성은 재조합 균주의 생산성을 많이 떨어뜨린다. 최적 발효 조건은 각각의 숙주-벡터 시스템, 사용한 배지, 생성물 등에 따라 크게 변한다. 동일한 숙주-벡터 시스템의 경우도, 사용하는 배지에 따라 온도, 희석률 또는 성장속도에 의존하는 정도가 달라지고 또 최적값도 다 변한다. 또한 발효조건의 최적화가 균체 내 플라스미드의 자기복제,mRNA로의 전사, 단백질로의 translation, 더 나아가 미생물 전체의 생리와 밀접하게 관련이 있다.

• Annual Conference of KIPS
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• 2020.11a
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• pp.912-915
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• 2020

최근 자연어 처리 기술은 지속적으로 발전하고 있으며, 많은 분야에서 활용되고 있다. 그 중 번역 기술은 가장 널리 사용되고 있는 자연어 처리 기술 중 하나이다. 본 논문에서는 기존의 seq2seq 모델의 단점을 극복하기 위해 개발된 Transformer 를 통해 영어-한국어 번역기를 만드는 것의 가능성을 제시한다.

• BMB Reports
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• v.29 no.4
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• pp.359-364
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• 1996

By both in vitro hydroxylamine mutagenesis of the wild type 3-phosphoglycerate kinase gene (PGK) promoter DNA and insertion of the leu2-d gene, we have created yeast expression vectors potentially useful for production of eukaryotic genes in yeast. The guanine (G) to adenine (A) change at the -3 position from the ATG start codon of the PGK promoter-based vector rendered a 6~7 times elevated expression of the adjacent eukaryotic gene, and insertion of the leu2-d gene in the vector containing the mutated PGK promoter further enhanced the expression of the gene. When expression of the AIDS virus HIV1-gagP17 gene in a lacZ fusion form was examined with this new vector, a 15 times higher level of expression than that from the original PGK promoter was observed. Northern and Southern analysis showed that this elevated expression is due to the production of a high copy number of mRNA by leu2-d gene functioning and by efficient translation of the produced mRNA. Thus, the vector that contained the A at the -3 position from the ATG start codon in the promoter region and the leu2-d gene shows increased expression capability and will be potentially useful for production of eukaryotic genes in yeast.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.26 no.12
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• pp.1919-1925
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• 2022

In this paper, we propose an image-to-image translation approach based on the conditional generative adversarial network for semantic segmentation. Semantic segmentation is the task of clustering parts of an image together which belong to the same object class. Unlike the traditional pixel-wise classification approach, the proposed method parses an input RGB image to its corresponding semantic segmentation mask using a pixel regression approach. The proposed method is based on the Pix2Pix image synthesis method. We employ residual connections-based convolutional neural network architectures for both the generator and discriminator architectures, as the residual connections speed up the training process and generate more accurate results. The proposed method has been trained and tested on the NYU-depthV2 dataset and could achieve a good mIOU value (49.5%). We also compare the proposed approach to the current methods in semantic segmentation showing that the proposed method outperforms most of those methods.

• Journal of The Korean Society of Integrative Medicine
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• v.7 no.2
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• pp.59-67
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• 2019

Purpose : The purpose of this study was to analyze the dynamic range of motion (ROM) of pelvic and translation of center of mass (COM) when wearing different shoe insole lifts according to leg length discrepancy (LLD) during free speed gait. Methods : Thirty-five healthy adults were participated in this study. Kinematic data were collected using a Vicon motion capture system. Reflective and cluster 40 markers attached to participants lower extremities and were asked to walk in a 6 m gait way under three different shoe lift conditions (without any insole, 1 cm insole, and 2 cm insole). The pelvic ROM and COM translation in three planes were sorted using a Nexus software, and a Visual3D motion analysis software was used to coordinate all kinematic data. Results : There were significantly increased maximal pelvic elevation and total pelvic range in coronal plane when wearing a standard shoe with 2 cm insole lift during gait (p<.05). When wearing a standard shoe with 2 cm insole lift, the total range of the pelvic segment were significantly different in all three motion planes (p<.05). Conclusion : Although LLD of less than 2 cm develops abnormal movement pattern of the pelvis and may cause of musculoskeletal diseases such as low back pain, hip and knee joint osteoarthritis, therefore intensive various physical therapy interventions for LLD are needed.

• Journal of Life Science
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• v.10 no.2
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• pp.150-156
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• 2000

This experiment was performed to evaluate the effect of TSH (thyroid-stimulating) on the ERp29 (endoplasmic reticulum resident 29 kDa protein) gene expression in the rat thyrocytes of FRTL-5 cells. Although ERp29 mRNA was constantly expressed, its expression began to increase remarkably from 10-9 M TSH. and its maximum expression was at 5×10-9 M TSH (about 3.5 fold). On the other hand, the effect of TSH on the abundance of ERp29 mRNA started within 6 h, and peaked at 8 h (about 2.5 fold). Actinomycin D (transcription inhibitor) strongly blocked this effect while cycloheximide (translation inhibitor) did not. The half-life of ERp29 mRNA was about 4.5 h in the presence or absence of TSH that was not affected by the stability of ERp29 mRNA. The effect of TSH on the ERp29 gene expression was specific, while other growth factors (transfferin, insulin, and hydrocortisone) did not alter its expression. Our data indicate for the first time that the expression of ERp29 is regulated transcriptionally by TSH in the thyrocytes.

• Journal of Microbiology and Biotechnology
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• v.4 no.2
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• pp.108-112
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• 1994

The genetic organization of the urease gene cluster from an alkalophilic Bacillus pasteurii was determined by subcloning and Tn5 transposon mutagenesis of a 10.7 kilobasepair cloned fragment. A region of DNA between 5.0 and 6.0 kb in length is necessary for urease activity. In vitro transcription-translation analysis of transposon insertion mutants of the cloned urease genes demonstrated that the major ($M_r$ 67,000) and minor ($M_r$ 20,000) structural peptides of urease are encoded at one end of the urease gene cluster and at least 3 additional polypeptides are encoded by adjacent DNA sequences.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.32B no.7
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• pp.989-1002
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• 1995

In this paper, we propose a new algorithm, so called the Signal Decorrelation via Virtual Translation of Array (SDVTA) algorithm, for estimating the directions of arrival(DOA's) of narrowband coherent signals incident on a general array. An effective procedure is composed of transforming the steering matrix of the original array into that of the virtually translated sensor array and taking the average of the transformed covariance matrices in order to decorrelate the coherent signals. The advantage of this approach is in that 1) it can estimate the DOA's of m-1 coherent signals(M : the number of array sensors) since the effective aperture size is never reduced. 2) a geometry of array is unrestricted for solving the narrowband coherency problem. 3) the efficiency of signal decorrelation does not depend on the phase differences between coherent signals unlike the Coherent Signal Subspace Method (CSM). Simulation results are illustrated to demonstrate the superior performance of this new algorithm in comparison with the normal MUSIC and examine the comparative performance with the various choices of the optimal transformation matrix under coherent signal environments.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.8
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• pp.1210-1214
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• 2002

The large and rapid changes of glucose utilization in lactating mammary tissue in response to changes in nutritional state must be largely related by external signal of insulin. This also must be related with the quantity and composition of the diet in vivo. To characterize the mode of gut extract protein with insulin, in vitro experiment was conducted with HC11 cells. The gut extract protein has not only the same effect as insulin alone but also the synergistic effect with insulin in 2-Deoxy[3H] glucose uptake. Although the gut extract did not modulates glucose uptake via increasing the rate of translation of the GLUT1 protein, northern blot analysis indicated that the gut extract protein increased the expression of GLUT1 mRNA by a threefold and also there was a dose-dependent increase in the expression of GLUT1 mRNA. The gut extract protein is therefore shown to be capable of modulating glucose uptake by transcription level with insulin in HC 11 cells.

• Journal of the Korean Institute of Telematics and Electronics S
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• v.35S no.10
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• pp.126-134
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• 1998

In this paper, we consider the problem of reconstructing a two-dimensional signal from its Fourier intensity and the Fourier intensities of two windowed signals: one by a window w(m,n) and the other by its complementary window [1-w(m,n)]. We develop several conditions under which a signal can be uniquely specified to within several trivial ambiguities such as sign, translation,and time-reversal from the given conditions. We present two possible reconstruction algorithms derived from the Gerchberg-Saxton algorithm.