• The Korean Journal of Physiology and Pharmacology
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• 제13권2호
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• pp.131-138
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• 2009

The binding of interleukin-6 (IL-6) cytokine family ligands to the gp130 receptor complex activates the Janus kinase (JAK)/ signal transducer and activator of transcription 3 (STAT3) signal transduction pathway, where STA T3 plays an important role in cell survival and tumorigenesis. Constitutive activation of STAT3 has been frequently observed in many cancer tissues, and thus, blocking of the gp130 signaling pathway, at the JAK level, might be a useful therapeutic approach for the suppression of STAT3 activity, as anticancer therapy. AG490 is a tyrphostin tyrosine kinase inhibitor that has been extensively used for inhibiting JAK2 in vitro and in vivo. In this study, we demonstrate a novel mechanism associated with AG490 that inhibits the JAK/STAT3 pathway. AG490 induced downregulation of gp130, a common receptor for the IL-6 cytokine family compounds, but not JAK2 or STAT3, within three hours of exposure. The downregulation of gp130 was not caused by enhanced degradation of gp130 or by inhibition of mRNA transcription. It most likely occurred by translation inhibition of gp130 in association with phosphorylation of the eukaryotic initiation factor-2 a. The inhibition of protein synthesis of gp130 by AG490 led to immediate loss of mature gp130 in cell membranes, due to its short half-life, thereby resulting in reduction in the STAT3 response to IL-6. Taken together, these results suggest that AG490 blocks the STAT3 activation pathway via a novel pathway.

• Mycobiology
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• 제38권1호
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• pp.7-12
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• 2010

A green mold species that has not previously been reported in Korea was isolated from oak log beds used for shiitake (Lentinula edodes) cultivation that were infested by mushroom flies. In this study, we identify the mold species as Gliocladium viride (an anamorph of Hypocrea lutea) and describe its mycological properties. The fungus was cottony on both potato dextrose agar (PDA) and Czapek yeast extract agar (CYA), but was colored white on PDA and became yellowish green and brown on CYA. Mycelial growth on PDA attained a diameter of 73 mm at $30^{\circ}C$ after 5 days. The fungus grew faster on malt extract agar (> 80 mm, 5 days at $25^{\circ}C$) compared to CYA and PDA (< 68 mm, 5 days at $25^{\circ}C$). Penicillate conidiophores of the fungus are hyaline, smooth walled, branching above typically in four stages, and $120\sim240\;{\mu}m$ in length. Club-shaped or slender phialides are formed on the metulae. Conidia of the fungus were ovate and elliptic, yellowish brown and green, and $2.5\sim3.0\;{\mu}m\times1.8\sim2.3\;{\mu}m$ in size. Typically, slimy conidia are formed in a mass and colored brown to dark green to almost black. The internal transcribed spacer rDNA and translation elongation factor 1 alpha gene sequences of the fungus isolated here show 99% identity with previously identified G. viride strains.

• Ocean Systems Engineering
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• 제2권3호
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• pp.205-215
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• 2012

Recently, interest of offshore structure construction in South Korea is growing as the land space becomes limited for further development and the renewable energy grows to be more attractive for the replacement of the fossil energy. In order for the optimal construction of optimum offshore floating structures, development of safe and economical offshore foundation technologies is a priority. In this study, the large-deformation behavior of a suction pile, which markets are rapidly growing nowadays, is analyzed for three different loading locations (top, middle, and bottom of the suction pile) with three different displacement inclinations (displacement controlled with displacement inclinations of 0, 10, and 20 degrees from the horizontal). The behavior analysis includes quantifications of maximum resistances, translations, and rotation angles of the suction pile. The suction pile with its diameter of 10 m and height of 25 m is assumed to be embedded in clay, sand, and multi layers of subsea foundation. The soil properties of the clay, sand, and multi layers were determined based on the results of the site investigations performed in the West sea of South Korea. As analyses results, the maximum resistance was observed at the middle of the suction pile with the displacement inclination of 20 degrees, while the translations and rotations resulting from the horizontal and inclined pullouts were not significant until the horizontal components of movements at the loading points reach 1.0 m.

• Asian-Australasian Journal of Animal Sciences
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• 제18권9호
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• pp.1326-1335
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• 2005

The main purpose of this study is to investigate the effects of dietary lysine deficiency on protein turnover of porcine muscles. There were 18 LYD three-breed-crossing postweanling barrows from six litters cannulated with gastric tubes through the esophagus at approximate 10 kg of body weight and allocated into three treatment groups. When their body weights reached over 12 kg, one group was sacrificed for determining the initial protein masses of m. masseter, m. longissimus dorsi, m. adductor and m. biceps femoris from the right body side. The others received a diet containing 100% or 61.4% (calculated values) of the lysine requirement (NRC, 1998) multiplied by 1.103 for a period of 17 days. Daily feed provision was computed for each pig according to body weight at the same day. All pigs were infused a flooding dose of $^2$H$_5$-phenylalanine to determine the fractional protein synthesis rates (FSR) of the aforementioned muscles in the end. Their four muscles from the right body side were also dissected for measuring the fractional rates of protein accretion (FAR). As for protein degradation, fractional rates (FDR) were calculated by differences between synthesis and accretion. Results showed that the lysine deficiency resulted in, significantly (p<0.05), lighter body weights, smaller muscles and a slower growth rate. The protein mass, accreted by the muscles, of the deficient group was only 54% averaged of the pigs fed adequately (p<0.05). The FAR of these muscles in the deficient group was significantly lower (p<0.05) and only achieved 61.1% averaged of the control; there was no significant difference (p>0.05), nevertheless, in the amino-acid composition of muscles between two groups. The lysine deficiency reduced significantly (p<0.05) the FSR of m. longissimus dorsi but did not influence its FDR. The m. biceps femoris also presented an inhibited FSR while its FDR reduced only exhibited a very high tendency (p = 0.055) compared to the adequately-fed pigs. As for the m. masseter and m. adductor, both of the FSR and FDR were depressed significantly (p<0.05) by the lysine deficiency, and changes in the FSR were severer than those in the FDR, so that their FAR were significantly slower (p<0.05) in comparison with the control group. The lysine deficiency also inhibited the RNA translation activity of the muscles while the effects on RNA capacity were not significant (p>0.05). In conclusion, the FAR of muscle protein was changed by the current lysine deficiency through the alterations in the FSR and/or FDR.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 한국마린엔지니어링학회 2001년도 추계학술대회 논문집(Proceeding of the KOSME 2001 Autumn Annual Meeting)
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• pp.112-119
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• 2001

Parameter tuning methods by Ziegler-Nickels for control systems are generally classified into Z-N(1) and Z-N(2). The purpose of this paper is to describe what relations exist between methods of Z-N(1) and Z-N(2), or how Z-N(1) method can be originated from Z-N(2) method by analyzing one loop control system of P or PI controller and time delay process. The formulas of Z-N(1) consist of process parameters, L(time delay), $K_m$(gain) and $T_m$(time constant), but Z-N(2) method is based only on the ultimate gain $K_u$ and the ultimate period $T_u$ acquired normally by practical trial without any parameters of Z-N(1). In this paper, for the first step to seek mutual relations, the simple formulas of Z-N(2) are transformed into the formulas composed of the same parameters as Z-N(1) which is derived from the analysis of frequency characteristics. Then, the approximation of the actual ultimate frequency is proposed as important premise in the translation between Z-N(1) and (2). Such equalization and approximation brings a simple approximated formula which can explain how Z-N(1) is originated from the Z-N(2) in the form of formula. And a model system is adopted to compare the approximated formula to Z-N(1) and Z-N(2) methods, the results of which show the effectiveness of the proposals.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• 제27권8호
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• pp.42-50
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• 2013

The reactionary responses to control human standing dynamics were estimated under the assumption that postural complexity mainly occurs in the mid-sagittal plane. During the experiment, the subject was exposed to continuous horizontal perturbation. The ankle and hip joint rotations of the subject mainly contributed to maintaining standing postural control. The designed mobile platform generated anterior/posterior (AP) motion. Non-predictive random translation was used as input for the system. The mean acceleration generated by the platform was measured as $0.44m/s^2$. The measured data were analyzed in the frequency domain by the coherence function and the frequency response function to estimate its dynamic responses. The significant correlation found between the input and output of the postural control system. The frequency response function revealed prominent resonant peaks within its frequency spectrum and magnitude. Subjects behaved as a non-rigid two link inverted pendulum. The analyzed data are consistent with the outcome hypothesized for this study.

• Biomedical Science Letters
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• 제20권1호
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• pp.1-7
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• 2014

RNA granules such as Stress Granules (SG) and P-Bodies (PB) are aggregates of translationally stalled messenger ribonucleoprotein (mRNP) complexes induced by a wide range of stresses. Over the past decade, extensive studies described key components of RNA granules, their molecular interactions and signaling pathways require for their assembly and disassembly. However, researches defining their exact roles under stress conditions have not been performed so far, although several studies suggested their roles in neurodegenerative diseases recently. In this review, we provide an introduction about their basic properties, key components, and the dynamic nature for their assembly.

• Annual Conference of KIPS
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• 한국정보처리학회 2010년도 추계학술발표대회
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• pp.786-788
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• 2010

최근 시맨틱 웹의 등장과 발전은 웹 2.0의 발전과 더불어 새로운 웹의 문화를 바꾸어 놓았다. 시맨틱 웹의 적용분야는 다양하지만 그중에서 의미 정보 검색과 다국어 정보 검색 기술을 통한 다국어 지원 번역이 연구 분야로의 필요성이 있다. 기존 기계번역이 번역률에 있어서 가장 큰 한계점은 단어 의미 중의성과 문법적은 오류이다. 따라서 본 논문에서는 시맨틱 웹과 단어 의미 중의성을 해소 시킬 새로운 알고리즘을 제안함으로써 단점을 제거하여 번역률을 향상시켜 모바일에 적용하였다. 모바일에 입력된 신문기사 이미지를 OCR을 통해 텍스트로 변환하고 사전 및 분야 온톨로지와 문장 규칙 추론을 동해 처리 속도 및 정확도 높은 번역시스템을 설계 및 구현하였다.

• Mycobiology
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• 제51권3호
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• pp.122-138
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• 2023

Elsinoë are plant pathogenic fungi that cause scabs, spotted anthracnose, and some morphological distortions on various plants, including woody plants, economically important crops, and ornamental plants. Taxonomical reexamination of Elsinoë species in Japan has not yet been conducted based on the modern species criteria. In this study, several Japanese isolates were reexamine based on the morphological and molecular-phylogenetic analysis of the internal transcribed spacer region (ITS), large subunit gene (LSU)m and protein-coding gene such as RNA polymerase II subunit (rpb2) and Translation elongation factor 1-alpha (tef). Japanese isolates were divided into four clades and three new species, Elsinoë hydrangeae, E. sumire, and E. tanashiensis were proposed. One species, Sphaceloma akebiae, was transferred to the genus Elsinoë.

• BMB Reports
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• 제56권2호
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• pp.49-55
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• 2023

Aging is characterized by a gradual decline in biological functions, leading to the increased probability of diseases and deaths in organisms. Previous studies have identified biological factors that modulate aging and lifespan, including non-coding RNAs (ncRNAs). Here, we review the relationship between aging and tRNA-derived small RNAs (tsRNAs), ncRNAs that are generated from the cleavage of tRNAs. We describe age-dependent changes in tsRNA levels and their functions in age-related diseases, such as cancer and neurodegenerative diseases. We also discuss the association of tsRNAs with aging-regulating processes, including mitochondrial respiration and reduced mRNA translation. We cover recent findings regarding the potential roles of tsRNAs in cellular senescence, a major cause of organismal aging. Overall, our review will provide useful information for understanding the roles of tsRNAs in aging and age-associated diseases.