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Kinetic Analyses for Enzymatic Properties of Trypsins Purified from Dark-Fleshed Fish (혈합육어 Trypsin의 효소적 성질에 대한 반응속도론적 해석)

  • CHO Deuk-Moon;HEU Min-Soo;KIM Hyeung-Rak;KIM Doo-Sang;PYEUN Jae-Hyeung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.1
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    • pp.64-70
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    • 1996
  • Kinetic properties of typsins purified from dark-fleshed fish (anchovy, mackerel, yellowfin tuna, and albacore) were examined and analyzed on $benzoyl-_{D,L}-arginine-p-nitroanilide\;(BAPNA)$. The values of Km' and $k_{cat}$ of the purified trypsins from the four dark-fleshed fish were found to be $49.3{\mu}M$ and $90.9\;min^{-1}$ for anchovy, $53.7{\mu}M$ and $61.2min-^{-1}$ for mackerel A, $96.5{\mu}M$ and $76.6min^{-1}$ for mackerel B, $62.8{\mu}M$ and $46.6min^{-1}$ for yellowfin tuna, and $98.3{\mu}M$ and $47.7min^{-1}$ for albacore, respectively. The values of $K_i$ on $tosyl-_L-lysine$ chloromethyl ketone (TLCK) were determined to be $20.90{\mu}M$ for anchovy trypsin, $2.86{\mu}M$ for mackerel trypsin A, $3.90{\mu}M$ for mackerel trypsin B, $0.96{\mu}M$ for yellowfin tuna trypsin, and $1.82{\mu}M$ for albacore trypsin. Thus yellowfin tuna trypsin was the most sensitive to TLCK among all trypsins. The activities and catalytic efficiency of the trypsins purified from the temperate zone fish, anchovy and mackerel, were higher than those of the trypsins purified from yellowfin tuna and albacore which migrate widely from the tropic zone to the temperate zone.

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Optical Properties of Sea Water in the Northwestern Waters of Jeju Island (제주도 북서해역에서의 해수의 광학적 성질)

  • 양용림
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.17 no.2
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    • pp.53-58
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    • 1981
  • Optical properties of sea water were studied in the northwestern water of Jeju Island, based on seven oceanographic stations in July, 1980. Submarine daylight intensity was measured at intervals of 5m depth in the upper 70m layer by using the underwater irradiameter(Kahlsico #268 WA360). The mean absorption coefficients of the sea water were appeared as 0.106(0.084-0.152), 0.135(0.106-0.184), 0.089(0.069-0.130) for clear, red, green, and blue color respectively. The transparency ranged from 11 to 19 meters(mean 16.1m). The mean water color in this area was 4.3(3-5) in Forel scales. The relation between absorption coefficient(k) and transparency(D) was k=1.66/D, k=2.12/D, k=1.38/D, and k=1.51/D for clear, red green, and blue color respectively. The rates of light penetration for clear, red, green, and blue color in four different depths were computed with reference to the surface light intensity respectively. The mean rates of light penetration in proportion to depths were as follows; clear : 56.57%(5m), 20.54%(15m), 4.60%(30m), 0.68%(50m). red : 50.14%(5m), 2.37%(30m), 0.23%(50m). green : 62.29%(5m), 26.43%(15m), 7.74%(30m), 1.56%(50m). blue : 59.29%(5m), 23.43%(15m), 6.10%(30m), 1.08%(50m).

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Antioxidative Activity of Rumex crispus L. Extract (소리쟁이 추출물의 항산화 효능)

  • Rhim, Tae-Jin;Choi, Moo-Young;Park, Hee-Juhn
    • Korean Journal of Plant Resources
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    • v.25 no.5
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    • pp.568-577
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    • 2012
  • The objective of this study was to investigate the antioxidative capacity of ethanol extracts from Rumex crispus L. The concentration of R. crispus L. extract at which DPPH radical scavenging activity was inhibited by 50% was 2.15 mg/mL, which was lower than that of ${\alpha}$-tocopherol (0.43 mg/mL), as compared to 100% by pyrogallol as a reference. Total antioxidant status was examined by total antioxidant capacity against ABTS radical reactions. Total antioxidant capacities of R. crispus L. extract at concentrations of 0.1 and 1 mg/mL were 0.47 and 2.33 mM Trolox equivalents, respectively, which were higher than those of ${\alpha}$-tocopherol. Superoxide scavenging activities of R. crispus L. extract at concentrations of 0.1 and 1 mg/mL were 21.5 and 78.9%, respectively, which were not significantly (p>0.05) different from those of catechin. Oxygen radical absorbance capacities of R. crispus L. extract at concentrations of 20 and 100 ${\mu}g/mL$ were 62.5 and 156.4 ${\mu}M$ Trolox equivalents, respectively, which were lower than those of ascorbic acid. Cupric reducing antioxidant capacities of R. crispus L. extract at concentrations of 0.1 and 1 mg/mL were 0.28 and 1.88 mM Trolox equivalents, which were similar or significantly (p<0.05) higher than those of ${\alpha}$-tocopherol, respectively. R. crispus L. extract prevented supercoiled DNA strand breakage induced by hydroxyl radical and peroxyl radical. Total phenolic contents of R. crispus L. extract at concentrations of 0.5 and 5 mg/mL were 0.58 and 3.85 mM gallic acid equivalents, respectively. R. crispus L. extract at concentration of 0.1 and 0.5 mg/mL inhibited 0.2 mM tert-butyl hydroperoxide-induced cytotoxicity by 38.5 and 63.5%, respectively, in HepG2 cell culture system. Thus, strong antioxidant and cytotoxicity-inhibiting effects of R. crispus L. extract seem to be due to, at least in part, the prevention from free radicals-induced oxidation as well as high levels in total phenolic contents.

Design of Systolic Multipliers in GF(2$^{m}$ ) Using an Irreducible All One Polynomial (기약 All One Polynomial을 이용한 유한체 GF(2$^{m}$ )상의 시스톨릭 곱셈기 설계)

  • Gwon, Sun Hak;Kim, Chang Hun;Hong, Chun Pyo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.8C
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    • pp.1047-1054
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    • 2004
  • In this paper, we present two systolic arrays for computing multiplications in CF(2$\^$m/) generated by an irreducible all one polynomial (AOP). The proposed two systolic mays have parallel-in parallel-out structure. The first systolic multiplier has area complexity of O(㎡) and time complexity of O(1). In other words, the multiplier consists of m(m+1)/2 identical cells and produces multiplication results at a rate of one every 1 clock cycle, after an initial delay of m/2+1 cycles. Compared with the previously proposed related multiplier using AOP, our design has 12 percent reduced hardware complexity and 50 percent reduced computation delay time. The other systolic multiplier, designed for cryptographic applications, has area complexity of O(m) and time complexity of O(m), i.e., it is composed of m+1 identical cells and produces multiplication results at a rate of one every m/2+1 clock cycles. Compared with other linear systolic multipliers, we find that our design has at least 43 percent reduced hardware complexity, 83 percent reduced computation delay time, and has twice higher throughput rate Furthermore, since the proposed two architectures have a high regularity and modularity, they are well suited to VLSI implementations. Therefore, when the proposed architectures are used for GF(2$\^$m/) applications, one can achieve maximum throughput performance with least hardware requirements.

쌍끌이 중층트롤어법의 연구 ( 1 ) - 모형어구의 망구형상에 관하여 - ( A Study on the Pair Midwater Trawling ( 1 ) - Mouth Performance of the Model Net - )

  • 이병기
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.31 no.1
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    • pp.29-44
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    • 1995
  • A model experiment on the pair midwater trawl net applicable to 800 PS class Korean pair bottom trawlers was carried out in the special-prepared experimental thank. the tank was prepared as a reverse trapezoid shape in its vertical section by digging out flat soil. The dimension of the tank showed the 9.6 W$\times$43.0 L(m) of the upper fringe and the 4.8 W$\times$38.0 L(m) of the bottom with 3.0m in depth. The depth of water was maintained 2.7m during experiment. The model net was prepared based on the Tauti's similarity law of fishing gear in 1/30 scale considering the dimension of the experimental tank. Mouth performance of the model net during towing were determined by the photographs taken in front of the net mouth with the combinations of towing velocity, warp length and distance between paired boats. The results obtained can be summarized as follows: 1. Vertical opening of the model nets A and B was varied in the range of 0.18~0.88 m and 0.21~0.78 m (which can be converted into 5.4~26.4m and 6.3~23.4 m in the full-scale net) respectively, and was varied predominantly by towing speed. Vertical opening (H which is appendixed m for the model net. f for the full-scale net. A and B for the types of the model net) can be expressed as the function of towing velocity$V_t$as in the model net $V_t$ : m/ sec)$H_{mA}$=1.67$e^{-1.65V_t}$ $H_{mB}$=1.15$e^{-1.13V_t}$, in the full-scale net ($V_t$ : k't) $H_{fA}$=50.27$e^-0.37V_t$ $H_{fB}$=34.46$e^{-0.26Vt}$. 2. Horizontal opening of the model nets An and b was varied in the range of 1.03~1.54m and 1.04~1.55 m (which can be converted into 30.9~46.2 m and 31.2~46.5m in the full-scale net) respectively, and was varied predominantly by distance between paired boats. Horizontal opening (W, appendixes are as same as the former) an be expressed as the function of distance between paired boats $D_b$as in the model net $W_{mA}$=0.69+0.09$D_b$ $W{mB}$=0.73+0.09$D_b$, in the full-scale net $W_{fA}$=20.81+0.09$D_b$ $W_{fB}$=22.11+0.09$D_b$ 3. Net opening area of the model net A and B was varied in the range of 0.28~1.04 $m^2$ and 0.33~0.94$m^2$(which can be converted into 252~936$m^2$ and 297~846$m^2$ in the full-scale net) respectively, and was varied predominantly by towing velocity. Net opening area ($S$, appendixes are as same as the former) van be expressed as the function of towing velocity$V_t$ as in the model net $v_t$ : m/sec) $S_{Ma}$=2.01$e^{-1.54V_T}$ $S_{mA}$=1.40$e^{-1.65V_t}$, in the full-scale net ($V_t$ : k't) $S_{fA}$=1.807$e^-0.35V_t$ $S_{fA}$=1.265$e^{-0.24V_t}$. 4. Filtering volume of the model nets A and B was varied in the range of 0.32~0.55 $m^3$ and 0.37~0.55$m^3$(which can be converted into 8.640~14.850 $m^3$ and 9.990~14.850$m3$in the full~scale net) respectively, and was predominantly varied by towing speed. filtering volume of the model net-A showed the maximum at the towing speed 0.69 m/sec(3 k't in the full-scale net), compared with that of the model net B showed at 0.92 m/sec(4 k't in the full-scale net).

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Effect of $K^+-channel$ Blockers on the $A_1-adenosine$ Receptor-Coupled Regulation of Electrically-Evoked Norepinephrine Release in the Rat Hippocampus (흰쥐 해마에서 Norepinephrine 유리를 조절하는 $A_1-adenosine$ 수용체의 역할에 미치는 $K^+$ 통로 차단제의 영향)

  • Choi, Bong-Kyu;Kim, Sang-Hoon
    • The Korean Journal of Pharmacology
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    • v.32 no.3
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    • pp.301-309
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    • 1996
  • Since it has been reported that the depolarization-induced NE release is inhibited by activation of presynaptic $A_1-adenosine$ heteroreceptor in hippocampus, a large body of experimental data on the post-receptor mechanism of this process has been accumulated. But, the post-receptor mechanism of presynaptic $A_1-adenosine$ receptor on the NE release has not been clearly elucidated yet. Therefore, it was attempted to clarify the participation of $K^+-channel$ in the post-receptor mechanisms of the $A_1-adenosine$ receptor-mediated control of NE release in this study. Slices from rat hippocampus were equilibrated with $^3H-norepinephrine$ and the release of the labelled products was evoked by electrical stimulation (3 Hz, 5 $VCm^{-1}$, 2 ms, rectangular pulses), and the influence of various agents on the evoked tritium-outflow was investigated. Adenosine, in concentrations ranging from $1{\sim}30\;{\mu}M$, decreased the NE release in a dose-dependent manner, without affecting the basal rate of release. 4AP $(1{\sim}30{\mu}M)$, a specific A-type $K^+-channel$ blocker, increased the evoked NE release in a dose-related fashion, and the basal rate of release is increased by 10 and $30{\mu}M$. TEA $(1{\sim}10{\mu}\;M)$, a nonspecific $K^+-channel$ blocker, increased the evoked NE release in a dose-dependent manner without affecting basal release. The adenosine effects were significantly inhibites by 3 ${\mu}M$ 4AP and 10 mM TEA treatment. 4AP $(30{\mu}M)-$ and TEA (10 mM)-induced increments of evoked NE release were completely abolished in $Ca^{++} free, but these were recoverd in low $Ca^{++} medium. And the effects of $K^+-channel$ blockers in low $Ca^{++} medium were inhibites and abolishes by $Mg^{++} (4 mM) adding and TTX $(0.3{\mu}M)$ adding medium, respectively. These results suggest that the decrement of the evoked NE-release by $A_1-adenosine$ receptor is mediated by 4AP and TEA sensitive $K^+-channel$.

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Design of double-clad, dispersion-compensating single-mode fiber with parabolic-index profile for $\lambda$=1.55 $\mu$m ($\lambda$=1.55 $\mu$m에서 포물선분포-이중클래드, 분산보상 단일모드 광섬유의 설계)

  • 김동각;김창민;이상배;강희전
    • Korean Journal of Optics and Photonics
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    • v.8 no.2
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    • pp.134-141
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    • 1997
  • We design the single mode dispersion-compensating fibers (DCF) which may be necessary for upgrading the previously installed 1.31 ${\mu}{\textrm}{m}$ optical communication system to the 1.55 ${\mu}{\textrm}{m}$ system. To obtain the optimum index profile that allows large negative dispersion at 1.55 ${\mu}{\textrm}{m}$, parabolic-index, double-clad fibers are analyzed by applying the 1-D FEM to the scalar wave equation of optical fibers. In constideration of macro-bending loss, the fibers are designed so that the cutoff wavelength of the $LP_{01}$ mode is greateer than 1.80 ${\mu}{\textrm}{m}$. The computer simulations show that the lower bound of the dispersion at 1.55 ${\mu}{\textrm}{m}$ is limited to about -120 ps/nm . km for the fiber index profiles satisfying the $LP_{01}$'s cutoff condition.

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Cryopreservation of Embryo by Concentration of Ethylene Glycol and Day 6, 7, 8, 9 Embryo in Korean Native Cattle (Hanwoo)

  • Park, Joung-Jun;Yoo, Han-Jun;Choi, Hye-Won;Cheong, Ki-Soo;Kim, Ji-Tae;Park, Choon-Keun;Yang, Boh-Suk
    • Journal of Embryo Transfer
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    • v.27 no.4
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    • pp.253-258
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    • 2012
  • This study was carried out to effects of ethylene glycol concentration, sucrose and culture day of in vitro production embryo on slow-down freezing in Hanwoo. 6, 7, 8 and 9 day embryos produced in vitro were frozen using 1.8M EG+0.1M sucrose, 1.8M EG+0.5% BSA and 1.5M EG+0.1M sucrose media. Survivability was confirmed after frozen-thawed 24 and 48h and ICM, TE cell number were counted by Hoechst 33342 and PI staining after frozen-thawed 24h. As a result, 1.8M EG+0.1M sucrose group was most significantly (p<0.05) higher compared with the other treatment groups on survivability, TE and total cell number after frozen-thawed 24h ($94.2{\pm}2.6%$, $94.67{\pm}3.4$ and $129.67{\pm}5.5$). ICM number did not found significant (p<0.05) differences between the three treatment groups. in 6, 7, 8 and 9 day of embryos using three types of freezing media, frozen-thawed, 1.8M EG+0.1M sucrose groups with embryos cultured 8 day was significantly (p<0.05) highest survivability to $98.3{\pm}1.7%$ after frozen-thawed 24h. 1.5M EG+0.1 sucrose group with embryos cultured 9 day was significantly higher survivability than group of embryos cultured 8 day after frozen-thawed 24 and 48h. In conclusion, 1.8M EG+0.1M sucrose media is considered to be effective to cryopreservation of embryos cultured 8 and 9 day.

Effect of Light Intensity, Temperature and $CO_2$ Concentration on Photosynthesis in Hovenia dulcis Thunb. (광도, 온도 및 $CO_2$의 농도가 헛개나무의 광합성에 미치는 영향)

  • Lee, Kang-Soo;Choi, Sun-Young
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.1
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    • pp.1-4
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    • 2002
  • This study was carried out to know the effect of light intensity, temperature and $CO_2$ concentration on photosynthesis and transpiration in Hovenia dulcis Thunb. Light compensation point was $2.4\;{\mu}mol\;m^{-2}\;s^{-1}$ and light saturation point was $1033\;{\mu}mol\;m^{-2}\;s^{-1}$. The optimum temperature for photosynthesis was $25^{\circ}C$ at $1000\;{\mu}mol\;m^{-2}\;s^{-1}$ light intensity. $CO_2$ compensation point was 67 vpm and $CO_2$ saturation point was 707 vpm. Transpiration rate was increased to about $2\;mmol\;m^{-2}\;s^{-1}$ with increasing of light intensity to $1750\;{\mu}mol\;m^{-2}\;s^{-1}$ and to above $4\;mmol\;m^{-2}\;s^{-1}$ with increasing of temperature from $18^{\circ}C$ to $36^{\circ}C$. however It was gradually reduced as $CO_2$ concentration increased from 21 vpm to 800 vpm.

Modeling Solar Irradiance in Tajikistan with XGBoost Algorithm (XGBoost를 이용한 타지키스탄 일사량 예측 모델)

  • Jeongdu Noh;Taeyoo Na;Seong-Seung Kang
    • The Journal of Engineering Geology
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    • v.33 no.3
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    • pp.403-411
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    • 2023
  • The possibility of utilizing radiant solar energy as a renewable energy resource in Tajikistan was investigated by assessing solar irradiance using XGBoost algorithm. Through training, validation, and testing, the seasonality of solar irradiance was clear in both actual and predicted values. Calculation of hourly values of solar irradiance on 1 July 2016, 2017, 2018, and 2019 indicated maximum actual and predicted values of 1,005 and 1,009 W/m2, 939 and 997 W/m2, 1,022 and 1,012 W/m2, 1,055 and 1,019 W/m2, respectively, with actual and predicted values being within 0.4~5.8%. XGBoost is thus a useful tool in predicting solar irradiance in Tajikistan and evaluating the possibility of utilizing radiant solar energy.