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QUANTIFICATION OF Fasciola gigantica INFESTATION IN ZEBU CATTLE OF BANGLADESH

  • Chowdhury, S.M.Z.H.;Mondal, M.M.H.;Huq, S.;Akhter, N.;Islam, M.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.3
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    • pp.343-346
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    • 1994
  • A research study was undertaken to quantify fascioliasis in both live and slaughtered zebu cattle at Savar, Bangladesh. Eggs of Fasciola gigantica per gram of feces (EPG) was determined in a total of 213 fasciola infested live cattle. The EPG per animal ranged from 100 to 400 (mean $138.03{\pm}4.27SE$). Counting of F. gigantica was made in a total of 63 fasciola infested livers of slaughtered cattle. Number of immature flukes per liver ranged from 0 to 37 (mean $8.74{\pm}0.85SE$) and mature flukes ranged from 2 to 121 (mean $20.54{\pm}2.23SE$). Total load of flukes recovered per liver varied from 4 to 132 (mean $29.28{\pm}2.42SE$). Significantly higher EPG (p < 0.05) and higher load of flukes in the livers (p < 0.01) were observed from September to December (post monsoon and winter). The EPG and fluke counts were found significantly higher (p < 0.01) in animals after one year of age and these were also higher in female animals (p < 0.05) than the males.

Studies on Density Measurement of Green Fe/Ni P/M Sheet Using ${\gamma}-ray$ (감마선을 이용한 소결 전 Fe/Ni 분말야금 판재의 밀도측정에 관한 연구)

  • Cho, K.S.;Lee, J.O.;Lee, S.Y.;Lee, J.S.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.12 no.3
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    • pp.7-11
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    • 1992
  • Accurate measurement of green density of compacted part in the powder metallurgy industry is rather fundamental but extremely important process that decide the quality of the sintered part. In case of green sheet P/M product, the green density as well as the distribution of the density must be examined for the same reasons. Currently in most cases, density measuring process is being performed applying conventional Archimedes principles. However this method is not only time-consuming but also often inaccurate because of the inherent nature of the process, such as part sectioning, closing of surface porosity with wax and weighing in air and in water. Therefore, it is necessary to develop a faster and more accurate method to measure the density of green sheet P/M product. In this work, a nondestructive density measurement device using gamma-ray absorption principles was constructed and the optimum condition for measuring green density of P/M sheet and its distribution was sought. The results showed that this method was very effective in terms of measuring time and accuracy.

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Retinol Supplemented to a Maturation Medium Stimulated In Vitro Development of Porcine Oocytes after In Vitro Fertilization (돼지 체외 수정란 생산 효율 제고를 위한 레티놀 첨가 효과)

  • Kim Y.S.;Song S.H.;Cho S.K.;Kwack D.O.;Kim C.W.;Park C.S.;Chung K.H.
    • Journal of Embryo Transfer
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    • v.21 no.2
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    • pp.101-107
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    • 2006
  • The objective of this study was to investigative the effects of retinol supplement to IVM and/or IVC medium on maturation, fertilization and development of pig oocytes. North Carolina State University (NCSU) 23 medium containing porcine follicular fluid (pFF) was used as base medium. Each 1 uM, 5 uM and 10 uM concentration of retinol was supplemented to IVM and /or IVC medium. When the retinol was supplemented to maturation medium, the maturation rates were not different (p>0.05) among treatment groups ($66.7{\pm}6.0{\sim}69.2{\pm}5.3%$), but the developmental rate to blastocyst stage was higher (p<0.05) in $5{\mu}M$ group ($20.4{\pm}2.6%$) than in 0 uM ($13.6{\pm}2.1%$) and 10 uM groups ($9.7{\pm}1.7%$). Moreover, total cell number was significantly greater (p<0.05) in the 5 uM group ($37.0{\pm}1.6$) than in the other groups ($29.8{\pm}1.0{\sim}33.2{\pm}1.0$). Retinol supplement to maturation medium did not significantly affect the rates of fertilization and polyspermy (p>0.05). When the retinol was supplemented to culture medium or both maturation and culture medium, the rates of cleavage, and develop to morula and blastocyst stage were not affected, while those of 10 uM group were significantly decreased (p<0.05). These results indicate that 5 uM retinol supplement in maturation medium significantly stimulates embryo development, also improves the total cell number of blastocyst stage in pig.

A Study on Smart Soil Resistance Measuring Device for Safety Characterized Ground Design in Converged Information Technology (ICT 융합 환경에서의 안전 특성화 접지 설계를 위한 스마트 대지 저항 측정 기술에 관한 연구)

  • Kim, Hong-Yong;Shin, Seung-Jung
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.19 no.1
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    • pp.203-209
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    • 2019
  • In this work, a new land-specific resistance measuring device (GM) and a measuring probe (Grounding Rod) are connected to the WENNER quadrant as power-line communication (PLC). In groups of two (P1,P2) probes, five to ten probes are installed in series on the ground at intervals of 1m, 2m, 4m, 8m, and 16m, respectively. If the PLC signal from the GMD is detected by the receiver of the Probe 1 (P1) for measurement, the minute voltage and current for measurement flow from the PSD (power supply) attached to the probe to the ground, and then, through the soil between P1 and P2, enters the Probe 1 (P2). The resistance value is then measured by the principle of voltage drop due to ground resistance. Measure the earth resistance every T seconds up to 1 trillion and store the measured data on the Arduino Server mounted on the main equipment. Stored measurement data can be derived from formulas by Ohm's Law and from inherent resistance (here,). Data obtained in real time will be linked to CDGES programs installed on Main PC, enabling data analysis and real-time monitoring of the ground environment on land. In addition, a three-dimensional display is possible with 3D graph support by identifying seasonal characteristics such as temperature and humidity of land (soils). The limitations of the study will require specific application measures of Test Bed for commercial access to a model that has been developed and operated experimentally.

Community Structure and Distributions of Zooplankton in Gangjin Bay in 1999 (1999년 강진만 동물플랑크톤 군집의 분포 및 특성)

  • 허회권;김도현;안승환
    • Korean Journal of Environmental Biology
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    • v.20 no.1
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    • pp.46-54
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    • 2002
  • We observed zooplankton community to understand variations in the species composition and abundance in Gangjin Bay. Samples were collected bimonthly from February to November 1999 at 10 stations in Gangjin Bay of the southern part of Korea. Zooplankton communities consisted to nine taxa and mean biomass was 2,028 indiv. $m^{-3}$. The maximum abundance was observed to be 5,496 indiv. $m^{-3}$ in February and the minimum in November, 78 indiv. $m^{-3}$. Copepods dominated and most diverse in Gangjin Bay. Seasonal fluctuation in the copepod abundance varied between 42 and 4,159 indiv. $m^{-3}$. Dominant species are Acartia omorii, Centrophages abdominalis, Paracalanus indicus and A. steueri. Cladoceran also dominated and the maximum abundance was 765 indiv. $m^{-3}$ in April. A. omorii and Oithona davisae dominated in February, A. omorii, Eurytemora pacifica, Evadne nordmanni and Podon polyphemoides in April, Tortanus dextrilobatus and decapod nauplius in June, Paracalanus indicus, E. tergestina and Penitia avirostris in August and A. erythraea and P. indicus in October. In November, P. indicus and Sagitta crassa dominated.

The Effects of mCIMT using PNF on the Upper Extremity Function and Activities of Daily Living in Patients with Subacute Stroke (고유수용성촉진법을 이용한 수정된 강제유도 운동치료가 아급성 뇌졸중 환자의 상지 기능과 일상생활수행능력에 미치는 영향)

  • Bang, Dae-Hyouk;Song, Myung-Soo;Cho, Hyuk-Shin
    • PNF and Movement
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    • v.16 no.3
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    • pp.451-460
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    • 2018
  • Purpose: The purpose of this study was to investigate the effects of a modified constraint induced movement therapy (mCIMT) using proprioceptive neuromuscular facilitation (PNF) on the upper extremity function and activities of daily living (ADLs) in patients with subacute stroke. Methods: Fourteen participants with subacute stroke were randomly assigned to a group using both mCIMT and PNF or a group using mCIMT alone. Each group underwent twenty sessions (1 h/d, 5 d/wk) for 4 weeks. Patients were assessed with the action research arm test (ARAT), the Fugl-Meyer assessment for the upper extremities (FMA-UE), the Modified Barthel Index (MBI), and motor activity logs (MALs; amount of use [AOU] and quality of movement [QOM]). Results: Both the experimental group and the control group showed significant intragroup improvement in the ARAT, FMA-UE, MBI, and MAL-AOU (p<0.05). The group using both mCIMT and PNF exhibited greater improvement in the ARAT, FMA-UE, MBI, and MAL-AOU than did the group using mCIMT alone. Statistical analyses showed significant differences in the ARAT (p=0.01), FMA-UE (p=0.01), MBI (p=0.00), and MAL-AOU (p=0.01) between the groups. Conclusion: This study applied mCIMT combined with PNF for subacute stroke patients, and the results showed significant improvements in the patients' upper extremity function and ADLs. Therefore, mCIMT using PNF may be more effective than mCIMT alone in improving upper limb function and ADLs in patients with subacute stroke.

Baby-Step 2k-ary Adult-Step Algorithm for Symmetric-Key Decryption (대칭키 해독을 위한 아기걸음 2k-ary 성인걸음 알고리즘)

  • Lee, Sang-Un
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.15 no.2
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    • pp.23-29
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    • 2015
  • The baby-step giant-step algorithm seeks b in a discrete logarithm problem when a,c,p of $a^b{\equiv}c$(mod p) are already given. It does so by dividing p by m block of $m={\lceil}{\sqrt{p}}{\rceil}$ length and letting one giant walk straight toward $a^0$ with constant m strides in search for b. In this paper, I basically reduce $m={\lceil}{\sqrt{p}}{\rceil}$ to p/l, $a^l$ > p and replace a giant with an adult who is designed to walk straight with constant l strides. I also extend the algorithm to allow $2^k$ adults to walk simultaneously. As a consequence, the proposed algorithm quarters the execution time of the basic adult-walk method when applied to $2^k$, (k=2) in the range of $1{\leq}b{\leq}p-1$. In conclusion, the proposed algorithm greatly shorten the step number of baby-step giant-step.

Distinctive pH Dependence and Substrate Specificity of Peptide Hydrolysis by Human Stromelysin-1 (Stromelysin-1에 의한 펩타이드 가수분해에서 pH와 기질특이성 연구)

  • ;Marianne V. Sorensen
    • Journal of Life Science
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    • v.10 no.2
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    • pp.210-217
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    • 2000
  • A kinetic profile of the catalytic domain of stromelysin-1 (SCD) using the fluorescent peptide substrate has been determined by the stopped-flow technique. The pH profile has a pH optimum of about 5.5 with an extended shoulder above pH 7. Three pKa values, 5.0, 5.7, and 9.8 are found for the free enzyme state and two pH independent Kcat/Km values of 4.1$\times$104 M-1 s-1 and 1.4$\times$104 M-1 s-1 at low and high pH, respectively. The profile is quite different in shape with other MMP family which has been reported, having broad pH optimum with two pKa values. The substrate specificity of SCD towards fluorescent heptapeptide substrates has been also examined by thin layer chromatography. The cleavage sites of the substrates have been identified using reverse-phase HPLC method.SCD cleaves Dns-PLA↓L↓WAR and Dns-PLA↓L↓FAR at two positions. However, the Dns-PLA↓LRAR, Dns-PLE↓LFAR, adn Dns-PLSar↓LFAR are cleaved exclusively at one bond. The double cleavages of Dns-PLALWAR and Dns-PLALFAR by SCD are in marked contrast to the close structurally related matrilysin. A notable feature of SCD catalysis agrees with the structural data that the S1' pocket of SCD is deeper than that of matriysin. The differences observed between SCD and matrilysin may form the basis of understanding the structural relationships and substrate specificities of the MMP family in vivo.

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Enhancement of Fertilizing Ability of Frozen-Thawed Bovine and Human Spermatozoa Treated with Fertilizing Promoting Peptide or Pentoxifylline (Fertilizing Promoting Peptide와 Pentoxifylline으로 처리된 소와 사람 동결 정액의 수정능 향상)

  • Lee, K.S.;Kim, E.Y.;Park, S.Y.;Shin, H.A.;Park, S.P.;Lim, J.H.;Chung, K.S.;Lee, H.T.
    • Korean Journal of Animal Reproduction
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    • v.25 no.4
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    • pp.409-419
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    • 2001
  • This study was to examine whether the in vitro friability, motility and intact acrosome of frozen-thawed bovine and human sperm can be improved by adding Pentoxifylline (PF) or Fertilization Promoting Peptide (FPP). Human semen was frozen ultra-rapidly using Test yolk-buffer (TYB) freezing medium. Additive (PF, FPP) effects in frozen-thawed bovine and human sperm were analyzed by microscopic count for sperm motility and coomassie brilliant blue staining method f3r sperm acrosome intact. The in vitro motility of frozen-thawed bovine sperm with 5 mM PF treatment group (50.0%) was significantly higher than that of control (34.0%) (P<0.05). In the frozen-thawed bovine sperm was examined, the intact acrosome rate of 50 nM FPP treatment (49.0%) was significantly higher than those of control (30.0%) and 25 nM FPP (38.0%) treatment groups (P<0.01). In human semen, when in vitro motility of sperm with PF addition prior to freezing was examined, the result of 5 mM treatment group (51.0%) was significantly higher than those of control and 2.5 mM treatment group (39.0, 40.0%) (P<0.01). In addition, 50 nM (75.5%) FPP adding in all treatment procedures for human semen freezing (before freezing, freezing and after thawing) was significant effect on maintenance of the sperm intact acrosome percentage (control: 45.0; 25 nM: 53.0; 100 nM: 68.0%) (P<0.01). Also, the intact acrosome rate of human sperm with FPP (65.0%) was significantly higher than that with PF (43.0%) (P<0.05), although sperm motility was slightly higher in PF treatment group. These results suggest that improved sperm motility and intact acrosome of frozen thawed bovine and human sperm can be obtained by addition of PF or FPP, and that the enhanced in vitro viability, motility and intact acrosome can be obtained by addition of FPP in all semen freezing procedures.

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The Association of Serum Ferritin and Metabolic Syndrome and Metabolic Syndrome Score in Korean Adults (대한민국 성인에서 대사증후군 및 대사증후군 구성요소의 증가와 혈청 Ferritin의 관련성)

  • Yoon, Hyun;Go, Jae Seong;Kim, Kang Uk;Lee, Keon Woo
    • Korean Journal of Clinical Laboratory Science
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    • v.48 no.4
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    • pp.287-295
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    • 2016
  • This study was conducted to assess the relationship amongst serum ferritin, and metabolic syndrome (MetS) and metabolic syndrome score (MSS) in Korean adults. The data of 16,096 adults (6,840 men as well as 4,916 premenopausal and 4,340 postmenopausal women) aged ${\geq}20years$ in the Korean National Health and Nutrition Examination Survey (KNHANES) between 2010 and 2012 were analyzed. The prevalence rate of MetS was 3,978 (24.7%) (men, 24.6%; premenopausal women, 11.1%; postmenopausal women, 40.3%). The key study results were as follows: First, after the adjustment for relevant variables, the serum ferritin level ($M{\pm}SE$) was significantly higher (p<0.001) in the MetS group (men, $132.25{\pm}1.98ng/mL$; premenopausal women, $39.89{\pm}1.49ng/mL$; postmenopausal women, $73.45{\pm}1.14ng/mL$) than in the non-MetS group (men, $111.08{\pm}1.01ng/mL$; premenopausal women, $32.26{\pm}0.50ng/mL$; postmenopausal women, $63.26{\pm}0.98ng/mL$). Second, the serum ferritin levels increased as MSS increased in all groups (men, premenopausal women, and postmenopausal women) (p<0.001). In conclusion, MetS and MSS increases were positively associated with higher serum ferritin levels.