• Title/Summary/Keyword: M. officinalis

검색결과 117건 처리시간 0.176초

Corynespora cassiicola에 의한 로즈마리 점무늬병 (Occurrence of Target Spot on Rosemary Caused by Corynespora cassiicola in Korea)

  • 이왕휴;한상준;최인영
    • 식물병연구
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    • 제19권1호
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    • pp.55-59
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    • 2013
  • 이 연구는 국내에 보고되지 않은 로즈마리 점무늬병이 남원과 전주의 재배 온실에서 발생하여 구명하고자 실험하였다. 이 병은 주로 고온 다습한 시기인 장마끝 무렵에 발생이 많았다. 로즈마리의 잎과 줄기에 검은점무늬(직경 3-5 mm) 및 시들음 증상이 나타났다. 병든 조직을 습실 처리하면 분생자경과 분생포자가 형성되었다. 로즈마리 점무늬병균의 분생포자는 체인상의 가늘고 긴 원통형으로, 길이는 $55-275{\times}7-14{\mu}m$이었다. 분생포자는 분생자경 위에 형성되었고, 분생포자의 위격벽의 수는 8-10개이었다. 또한 생장적온은 감자한천 배지와 암흑조건에서 $30^{\circ}C$이었다. 포트 식물에 병원성 검정결과 분리한 병원균은 접종 3일 후에 잎과 줄기에서 자연 병징과 같은 점무늬 및 시들음 증상이 재현되었고, 병든 부위에서 동일한 균이 재 분리되었다. 따라서 병원성이 있음이 확인되었다. rDNA ITS 영역의 염기서열 분석결과 로즈마리에 점무늬병을 일으키는 병원균은 Corynespora cassiicola로 GenBank accession number JQ595296, JQ595297, FJ852715, AY238606와 염기서열이 100% 일치하였고, C. cassiicola와 같은 계통군에 속하였다. 따라서, 로즈마리 잎 및 가지 점무늬병균은 C. cassiicola로 보고하고자 한다.

Anti-Aspergillus Activities of Plant Essential Oils and Their Combination Effects with Ketoconazole or Amphotericin B

  • Shin, Seung-Won
    • Archives of Pharmacal Research
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    • 제26권5호
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    • pp.389-393
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    • 2003
  • The essential oils from Cedrus atlantica, Styrax tonkinensis, Juniperus communis, Lavandula angustifolia, Melaleuca alternifolia, Pelargonium graveolens, Pogestemon patchouli and Rosmarinus officinalis were analyzed by GC-MS. Antifungal activities of the oils were investigated by disk diffusion assay and the broth dilution method against Aspergillus niger and A. flavus. The effects of geraniol and the essential oil fraction from P. graveolens on the antifungal activity of amphotericin Band ketoconazole were examined using a checkerboard microtiter assay against both Aspergillus fungi. Most of the tested essential oils, with the exception of C. atlantica, J. communis, and P. patchouli, significantly inhibited growth of A. niger and to a lesser extent that of A. fIavus, with MICs (minimal inhibitory concentrations) in the range 0.78-12.5 mg/mL. The essential oil fraction of P. graveolens and its main components, geraniol and citronellol, exhibited additive effects with amphotericin B and with ketoconazole against both Aspergillus species, resulting in fractional inhibitory concentration (FIC) indices ranging from 0.52 to 1.00.

Zygotorulaspora cornina sp. nov. and Zygotorulaspora smilacis sp. nov., Two Novel Ascomycetous Yeast Species Isolated from Plant Flowers and Fruits

  • Ahn, Chorong;Kim, Minkyeong;Kim, Changmu
    • Mycobiology
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    • 제49권5호
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    • pp.521-526
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    • 2021
  • Three isolates belonging to the ascomycetous genus Zygotorulaspora were obtained from the fruits of Cornus officinalis and Smilax china, and flowers of Dendranthema zawadskii var. latilobum in Gongju-si, Korea. Phylogenetic Analyses of the LSU D1/D2 domain and ITS region sequences supported the recognition of two new species: Zygotorulaspora cornina sp. nov. (type strain NIBRFGC000500475 = KACC93346PPP) and Zygotorulaspora smilacis sp. nov. (type strain NIBRFGC000500476 = KACC93347PPP). The two novel species revealed no growth on D-Galactose, unlike the other six species in the genus Zygotorulaspora. They are distinguished from each other by their phylogenetic differences and phenotypic characteristics such as assimilation of xylitol, 5-keto-D-gluconate, and ethanol. All species in the genus Zygotorulaspora including the two novel species have phenotypic traits of genus Zygotorulaspora: asci are persistent, sucrose and raffinose are assimilated, and m-inositol is not required for growth, and they are mainly associated with plants.

독도의 해조상 및 군집구조 (Marine Algal Flora and Community Structure in Dokdo, East Sea, Korea)

  • 최창근;이해원;홍병규
    • 한국수산과학회지
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    • 제42권5호
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    • pp.503-508
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    • 2009
  • We investigated species composition and variation of benthic marine algae between 2003 and 2006 at Dokdo on the East Sea. The total number of benthic algae species identified was 96, which included 15 Chlorophyta, 30 Phaeophyta, 50 Rhodophyta and 1 Spermatophyta species. The representative species in this study were Ulva pertusa, Caulerpa okamurae, Codium adhaerens, C. frgile, Undaria pinnatifida, Ecklonia cava, Eisenia bicyclis, Dictyopteris undulata, Padina arborescens, Sargassum horneri, Hildenbrandtia rubra, Lithophyllum okamurae, Amphiroa dilatata, Corallina officinalis, C. pilulifera, Prionitis cornea, Grateloupia elliptica, Plocamium telgairiae, Chondrus ocellatus, Chondracanthus intermedia, Chondria crassicaulis, Polysiphonia morrowii, Melobesioidean algae and Phyllospadix sp. Wet weight biomass according to various depths ranged between 1,094.8 to $2,595.3\;g\;m^{-2}$ during the study period. Mean biomass at the investigated sites was greater in the 15m depth range than in the 5 and 10m depths. Vertical distribution was characterized by Ulva intestinalis, U. linza, Endarachne binghamiae, Bangia atropurpurea, Gloiopeltis furcata and Chondria crassicaulis at intertidal zone, Amphiroa dilatata, Corallina pilulifera, Gelidium amansii, Eisenia bicyclis and Ecklonia cava at 2m depth, Ecklonia cava, Eisenia bicyclis, Padina arborescens, Undaria pinnatifida and Sargasum horneri at 5m depth, Ecklonia cava, Sargassum horneri and Padina arborescens at 10m depth, Ecklonia cava, Myagropsis myagroides, Padina arborescens and Sargassum horneri at 15m depth. The R/P, C/P and (R+C)/P value were 1.67, 0.50 and 2.17, respectively.

더덕에 발생하는 당근뿌리혹선충의 증식억제 식물 탐색 (Screening of Medicinal Plants to Suppress Population of Meloidogyne hapla in Codonopsis lanceolata Trautv)

  • 임주락;황창연;김대향;최정식
    • 한국응용곤충학회지
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    • 제45권3호
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    • pp.339-346
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    • 2006
  • 비름과 쇠무릎등 총 36과 81속 90종의 약용식물을 대상으로 당근뿌리혹선충의 감염 여부를 조사한 결과, 쇠무릎 등 70종의 식물에서 당근뿌리혹선충 감염을 확인하지 못했고, 이들 70종 중 42종의 식물을 당근뿌리혹선충에 의한 더덕의 피해가 많았던 포장에 심고, 감염여부를 조사한 결과, 쇠무릎 등 26종은 당근뿌리혹선충에 거의 감염되지 않았고, 지치 등 16종은 감염되었음을 확인하였다. 또한 42종의 식물중 30종의 식물을 Pot 재배를 통하여 당근뿌리혹선충 접종밀도별 뿌리혹수와 토양 내 유충밀도를 조사한 결과 패랭이, 원추천인국, 기린초, 아주까리, 지모, 해변아욱, 애기똥풀, 흑참깨, 짚신나물, 큰뱀무, 오이풀, 현삼 등 12종은 당근뿌리혹선충에 거의 감염되지 않았음을 확인하였다. 반면에 더덕은 당근뿌리혹선충 접종밀도가 높을수록 뿌리혹수가 많아지고, 토양 내 유충밀도 역시 높아졌으며, 이에 따라 더덕의 생육은 접종밀도가 높을수록 나빠지는 경향이었다.

207종 한약과 활성 한약이 포함된 19종 한약 방제의 1,1-Diphenyl-2-Picrylhydrazyl 라디칼 소거작용 (The Scavenging Effect of 207 Oriental Medicines and 19 Oriental Prescriptions on 1,1-Diphenyl-2-Picrylhydrazyl Radical)

  • 김민산;허종문;박종철
    • 대한한의학방제학회지
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    • 제12권1호
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    • pp.159-175
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    • 2004
  • The inhibitory effects of 207 oriental medicines and 19 oriental prescriptions on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical were examined. The most effective oriental medicines were the fruit of Terminalia chebula, followed in order by the seed of Areca catechu, the heart wood of Caesalpinia sappan, the pericarp of Castanea crenata, the seed of Nelumbo nucifera, Rhus galls, the root of Sanguisorba officinalis, and the bark of Betula platyphylla at the concentration of 10 ${\mu}g$/mL. Of the 19 oriental prescriptions, Buyeonsan and Samsinhwan showed the potent scavenging activities of 88% and 71% on DPPH radical at the concentration of 5 ${\mu}g$/mL, respectively.

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Effect of Metformin on Cell Growth and Differentiation in Cultured Odontoblasts

  • Oh, Chang Young;Kim, Su-Gwan;Go, Dae-San;Yu, Sun-Kyoung;Kim, Tae-Hoon;Kim, Chun Sung;Park, Joo-Cheol;Kim, Do Kyung
    • International Journal of Oral Biology
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    • 제42권2호
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    • pp.39-45
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    • 2017
  • Metformin (1,1-dimethylbiguanide hydrochloride), derived from French lilac (Galega officinalis), is a first-line anti-diabetic drug prescribed for patients with type 2 diabetes. However, the role of metformin in odontoblastic cell differentiation is still unclear. This study therefore undertook to examine the effect of metformin on regulating odontoblast differentiation in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. As compared to controls, metformin significantly accelerated the mineralization, significantly increased and accelerated the expressions of ALP and Col I mRNAs, and significantly increased the accelerated expressions of DSPP and DMP-1 mRNAs, during differentiation of MDPC-23 cells. There was no alteration in cell proliferation of MDPC-23 cells, on exposure to metformin. These results suggest that the effect of metformin on MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells, facilitates the odontoblast differentiation and mineralization, without altering the cell proliferation.

후박의 품질평가 (Quality Evaluation on Magnoliae Cortex)

  • 배기환;김영호;원도희;이준성;강종성
    • 약학회지
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    • 제41권4호
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    • pp.407-413
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    • 1997
  • Magnolol and honokiol, the main components of Magnoliae Cortex, were isolated and used as the standard substances for the analysis. In order to determine the contents of magnolol and honokiol in Magnoliae Cortex originated from Korea, China and Japan, both HPLC and HPTLC methods are applied and compared with each other. The components were separated on C8 column with acetonitrile-water-acetic acid (50:50:1) in HPLC and detected at UV 294nm. The components separated on HPTLC precoated silica gel plate with chloroform-methanol (9:1) were detected directly on the plate at 254nm. The contents of magnolol and honokiol in Magnoliae Cortex were in the wide range of 0.01~2.8% and 0.005~0.8%, respectively, according to their purchase places. It is also applicable to the quality control of various preparation from Magnoliae Cortex.

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Flos magnoliae constituent fargesin has an anti-allergic effect via ORAI1 channel inhibition

  • Hong, Phan Thi Lam;Kim, Hyun Jong;Kim, Woo Kyung;Nam, Joo Hyun
    • The Korean Journal of Physiology and Pharmacology
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    • 제25권3호
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    • pp.251-258
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    • 2021
  • Flos magnoliae (FM), the dry flower buds of Magnolia officinalis or its related species, is a traditional herbal medicine commonly used in Asia for symptomatic relief of and treating allergic rhinitis, headache, and sinusitis. Although several studies have reported the effects of FM on store-operated calcium entry (SOCE) via the ORAI1 channel, which is essential during intracellular calcium signaling cascade generation for T cell activation and mast cell degranulation, the effects of its isolated constituents on SOCE remain unidentified. Therefore, we investigated which of the five major constituents of 30% ethanoic FM (vanillic acid, tiliroside, eudesmin, magnolin, and fargesin) inhibit SOCE and their physiological effects on immune cells. The conventional whole-cell patch clamp results showed that fargesin, magnolin, and eudesmin significantly inhibited SOCE and thus human primary CD4+ T lymphocyte proliferation, as well as allergen-induced histamine release in mast cells. Among them, fargesin demonstrated the most potent inhibitory effects not only on ORAI1 (IC50 = 12.46 ± 1.300 μM) but also on T-cell proliferation (by 87.74% ± 1.835%) and mast cell degranulation (by 20.11% ± 5.366%) at 100 μM. Our findings suggest that fargesin can be a promising candidate for the development of therapeutic drugs to treat allergic diseases.

추출용매에 따른 레몬밤 추출물의 중성지방 조절 효능 검증 (Verification of the Effect of Lemon Balm Extract on Triglyceride Control According to the Extraction Solvent)

  • 김지연;김경곤;이혜림;김대중;김태우
    • 한국자원식물학회지
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    • 제35권2호
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    • pp.372-379
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    • 2022
  • 본 연구는 추출용매에 따른 레몬밤 추출물의 중성지방 조절에 대한 논문으로 3T3-L1 지방전구세포를 이용해 지방세포로 분화유도한 뒤 lipid accumulation, triglyceride contents, PKA, HSL, perilipin, ATGL 및 CGI-58 단백질 발현을 확인하였다. 실험결과 네가지 조건의 레몬밤 추출물 중 물 추출 조건인 MOW100 추출물만 lipid accumulation과 triglyceride contents의 유의적 억제 효능을 나타내었으며, MOE70, MOE50, MOE50 추출물은 lipid accumulation과 triglyceride contents의 감소 효과가 없거나 유의적 차이를 나타내지 않는 것이 확인되었다. MOW100 추출물의 세포내 지방 축적 억제는 지방세포 내 lipolysis를 조절하는 효소 조절을 통해 나타난 결과로 판단된다. 따라서 레몬밤 물 추출물은 cAMP-PKA를 활성화시키고, lipolysis를 조절하는 효소의 상승작용을 통해 세포내 중성지방을 조절함으로써 혈중 중성지방을 개선하는 후보소재로의 사용이 가능할 것으로 사료된다.