• Molecules and Cells
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• v.37 no.1
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• pp.17-23
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• 2014

We already had reported that Bcl-w promotes invasion or migration in gastric cancer cells and glioblastoma multiforme (GBM) by activating matrix metalloproteinase-2 (MMP-2) via specificity protein 1 (Sp1) or ${\beta}$-cateinin, respectively. High expression of Bcl-w also has been reported in GBM which is the most common malignant brain tumor and exhibits aggressive and invasive behavior. These reports propose that Bcl-w-induced signaling is strongly associated with aggressive characteristic of GBM. We demonstrated that Sp1 protein or mRNA expression is induced by Bcl-w using Western blotting or RT-PCR, respectively, and markedly elevated in high-grade glioma specimens compared with low-grade glioma tissues using tissue array. However, relationship between Bcl-w-related signaling and aggressive characteristic of GBM is poorly characterized. This study suggested that Bcl-w-induced Sp1 activation promoted expression of glioma stem-like cell markers, such as Musashi, Nanog, Oct4 and sox-2, as well as neurosphere formation and invasiveness, using western blotting, neurosphere formation assay, or invasion assay, culminating in their aggressive behavior. Therefore, Bcl-w-induced Sp1 activation is proposed as a putative marker for aggressiveness of GBM.

• Journal of the Korean Society for Heat Treatment
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• v.10 no.1
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• pp.1-9
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• 1997

The variation of the mechanical properties and the formation of retained austenite with heat treatment conditions in austempered Si bearing carbon steels has been investigated. In the case of a steel containing 0.35C-1.48Si-0.95Mn, it has been found that a feather shape bainite structure of lath are obtained under a isothermal treated condition at just below the Ms temperature, and the martensite, bainitic ferrite and retained austenite of second phase particles on the ferrite matrix for a isothermal treated steels after intercritical annealing are precipitated in a linked shape. The retained austenite with $2{\mu}m$ size induced as TRIP is found to increase with increasing the formation rate of retained austenite for the intercritical annealing and high Si containing steels. The tensile strength is increased as austempering temperature increases in all isothermal treatment temperature, whereas the elongation is shown to roughly decrease as the tensile strength increases. The values of tensile strength-elongation balance have showed a marked dependence upon the elongation rather than the tensile stregth, and their values are increased for high Si containing steels and intercritical annealing condition. The most optimum result has been shown to be the tensile stregth-elongation balance of $2882.4kgf/mm^2.%$ and the elongation of 33.3% for a "B" steel in the heat treating temperature range of $780{\sim}370^{\circ}C$.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 1997.11a
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• pp.130-137
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• 1997

Intumescent inorganic and organic coatings which dintr one from the other by the type of gas formers and the mechanisms of foam formation have been obtained and investigated. Inorganic intumescent coatings are the compositions based on water glass and mineral additives with different dispersity. Mineral additives contain adsorbed and absorbed water and carbonates which are destructed with the carbon dioxide and water evolution during the flame action on coating. The decreasing of mineral additives particle sizes under the mechanical milling with the fraction precipitation promotes the foam coke formation with less defects. Here the main structure of comparing compositions does not change. In organic coatings based on epoxy-polymers the polyammonium phosphate additive is used. It is the cabonization catalyst and the foam agent. The polyammonium phosphate of various dispersity employed is uniformly distributed on the polymeric matrix. The decreasing of the particle sizes leads to the increasing of the fire resistant properties of the intumescent coa-ting. The fire resistant analysis of the coating during more than an hour: the coating back side the temperature on plastic or wooden materials does not exceed 423K, and on metal-573K.

• Annals of dermatology
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• v.30 no.6
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• pp.694-700
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• 2018

Background: Kaempferol (3,4',5,7-tetrahydroxyflavone) is a flavonoid known to have a wide range of pharmacological activities. The 3-OH group in flavonoids has been reported to determine antioxidant activities. Objective: We tested whether kaempferol can affect the expression of integrins and the stem cell fate of interfollicular epidermal stem cells. Methods: Skin equivalent (SE) models were constructed, and the expression levels of stem cell markers and basement membrane-related antigens were tested. The immunohistochemical staining patterns of integrins, p63, and proliferating cell nuclear antigen (PCNA) were compared between kaempferol- and apigenin-treated SE models. Reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate the mRNA expression of integrins. Results: Kaempferol increased the thickness of the epidermis when added to prepare SEs. In addition, the basal cells of kaempferol-treated SEs appeared more columnar. In the immunohistological study, the expression of integrins ${\alpha}6$ and ${\beta}1$ and the numbers of p63- and PCNA-positive cells were markedly higher in the kaempferol-treated model. However, apigenin showed no effects on the formation of three-dimensional skin models. RT-PCR analysis also confirmed that kaempferol increased the expression of integrin ${\alpha}6$ and integrin ${\beta}1$. Conclusion: Our findings indicated that kaempferol can increase the proliferative potential of basal epidermal cells by modulating the basement membrane. In other words, kaempferol can affect the fate of interfollicular epidermal stem cells by increasing the expression of both integrins ${\alpha}6$ and ${\beta}1$. These effects, in particular, might be ascribed to the 3-OH group of kaempferol.

• Journal of Radiation Industry
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• v.5 no.4
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• pp.325-330
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• 2011

To predict the details of flow in industrial process unit, single photon emission computed tomography (SPECT) is a promising technique. Recently, industrial SPECT based on medical system has developed by researchers of the Korea Atomic Energy Research Institute (KAERI) and Hanyang University. In the present study, to confirm the effect of the number of detectors on image quality, and determine the optimal number of detectors in industrial SPECT, industrial SPECT system with various geometries were evaluated by the Monte Carlo simulation. CsI(Tl) detectors ($12mm{\times}12mm{\times}20mm$) with collimators (the geometric resolution of collimator $R_g$ was 4 cm at the center of the 30 cm diameter cylindrical vessel object) were modeled in a hexagonal array, and the point sources of $^{99m}Tc$, $^{68}Ga$, and $^{137}Cs$ were simulated at the center of the cylindrical vessel object using the MCNPX code. Then, the reconstruction images of each geometry were reconstructed using the expectation maximization (EM) algorithm. In this study, the reciprocity theorem was used to improve computation time required for system matrix of the EM algorithm. The result shows that the resolution of the reconstructed image was significantly improved by increasing the number of detectors in industrial SPECT system and more than 60 detectors will be required for the resolution of the reconstructed image.

• Korean Journal of Metals and Materials
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• v.49 no.9
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• pp.692-698
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• 2011

The thermo-chemical treatment (TCT) process was applied to achieve surface hardening of CP titanium. The following three different surface modification conditions were tested so that the best surface hardening process could be selected:(a) PVD, (b) TCT+PVD, and (c) TCT+Aging+PVD. These specimens were tested and analyzed in terms of surface roughness, wear, friction coefficient, and the gradient of hardening from the surface of the matrix. The three test conditions were all beneficial to improve the surface hardness of CP titanium. Moreover, the TCT treated specimens, that is, (b) and (c), showed significantly improved surface hardness and low friction coefficients through the thickness up to $100{\mu}m$. This is due to the functionally gradient hardened surface improvement by the diffused interstitial elements. The hardened surface also showed improvement in bonding between the PVD and TCT surface, and this leads to improvement in wear resistance. However, TCT after aging treatment did not show much improvement in surface properties compared to TCT only. For the best surface hardening on CP titanium, TCT+PVD has advantages in surface durability and economics.

• Bulletin of the Korean Chemical Society
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• v.10 no.6
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• pp.582-585
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• 1989

Two crystal structures of dehydrated $Sr^{2+}\;and\;Tl^+$ exchanged zeolite A, $Sr_xTl_{12-2x}-A$ (x = 1.6 and 5.45), have been determined by single-crystal X-ray diffraction techniques. Their structures were solved and refined in the cubic space group Pm3m at $21(1)^{\circ}C.$ Both crystals were ion exchanged in flowing streams of mixed $Sr(NO_3)_2\;and\;TlNO_3$ aqueous solution, followed by dehydration at $360^{\circ}C\; and\; 2${\times}$10^{-6}$ Torr for 2 days. Full-matrix least-squares refinements of the dehydrated $Sr_{1.6}Tl_{8.8}-A (a = 12.214(2){\AA})\; and\;Sr_{5.45}Tl{1.1}-A (a=12.291(2){\AA})$ have converged to final error indices, $R_1=0.055\; and\;R_2=0.061$ with 286 reflections, and R1 = 0.072 and R2 = 0.090 with 217 reflections, respectively, for which$\;I\;{>}\;3{\sigma}(I)$. In both structures, all Sr(II) ions are coordinated by three framework oxygens; Sr(II) to O(3) distances are $2.21(2){\AA}\;for\;Sr_{1.6}Tl_{8.8}-A \;and\;2.31(1){\AA} \;for\;Sr_{5.45}Tl_{1.1}-A,$and Tl(I) to O(3) distances are $2.657(6){\AA}\;for\;Sr_{1.6}Tl_{8.8}-A\;and\;2.845(8){\AA}\;for\;Sr_{5.45}Tl_{1.1}-A,$ respectively. In each structure, the angle subtended at Sr(II), O(3)-Sr(II)-O(3) is $118.7(4)^{\circ}\;for\; Sr_{1.6}Tl_{8.8}-A \;and\;120.0(4)^{\circ}\;for\;Sr_{5.45}Tl_{1.1}-A.\;Sr^{2+}$ ions prefer to 6-ring sites and $Tl^+$ ions to 8-ring sites when total number of ions per unit cell is more than 8.

• Analytical Science and Technology
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• v.34 no.2
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• pp.37-45
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• 2021

A rapid and simple LC-MS/MS analytical method in determining Jaspine B has been developed and validated in rat plasma. The standard curve value was 25 - 5000 ng/mL and the linearity, inter-day and intra-day accuracy and precision were within 15.0 % of relative standard deviation (RSD). The mean recoveries of Jaspine B ranged from 87.5 % to 91.2 % with less than 3.70 % RSD and the matrix effects ranged from 91.1 % to 108.2 % with less than 2.6 % RSD. The validated LC-MS/MS analytical method of Jaspine B was successfully applied to investigate the dose-escalated pharmacokinetic study of Jaspine B in rats following an intravenous injection of Jaspine B at a dose range of 1 - 10 mg/kg. The initial plasma concentrations and area under plasma concentration curves showed a good correlation with intravenous Jaspine B dose, indicating the dose independent pharmacokinetics of Jaspine B in rats. In conclusion, this analytical method for Jaspine B can be easily applied in the bioanalysis and pharmacokinetic studies of Jaspine B, including its administration at multiple therapeutic doses, or for making pharmacokinetic comparisons for the oral formulations of Jaspine B in small experimental animals as well as in vivo pharmacokinetic-pharmacodynamic correlation studies.

• Food Engineering Progress
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• v.15 no.3
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• pp.203-208
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• 2011

Effects of particle size and high pressure processing on the extraction rate of oil compounds from soybean powder were evaluated by Soxhlet method using hexane and supercritical fluid extraction (SFE) using $CO_{2}$. SFE was carried out at 4,000 psi and $50^{\circ}C$ for 4 hr. The mean particle sizes were varied from 26.7 to 862.0 ${\mu}m$ by controlling milling time. Saturation solubility increased as the particle size decreased. At large particle size, high pressure processing (HPP) showed higher extraction yield in both hexane extraction and SFE, but, as the particle size decreased, the HPP was irrelevant to the extraction yield in SFE. The higher extraction rate obtained from the smaller particle size. The scanning electronic microscopy of soybean powder treated by HPP showed pores on the surface of the particle. The higher extraction rate and yield from HPP treatment might be due to the less internal resistance of transferring the solvent and miscellar in the solid matrix by collapsing of tissues.

• International Journal of Oral Biology
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• v.44 no.2
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• pp.55-61
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• 2019

The purpose of this study was to evaluate the effect of mangosteen extract complex (MEC; Garcinia mangostana L. and propolis extracts) on the inhibition of inflammation and prevention of alveolar bone loss using a ligature-induced periodontitis model. Rat molars were ligatured with silk, and $1{\mu}g/mL$ of lipopolysaccharide of Porphyromonas gingivalis was injected into the buccal and palatal gingivae of the teeth with or without treatment with the MEC. Changes in the expression levels of prostaglandin $E_2$ ($PGE_2$), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), matrix metalloproteinase-8 (MMP-8), cyclooxygenase (COX)-1, and COX-2 in gingival tissues were evaluated using enzyme-linked immunosorbent assays. Alveolar bone loss around the ligated molars was examined using micro-computed tomography. The expression levels of $PGE_2$, IL-8, iNOS, MMP-8, COX-1, and COX-2 in gingival tissues were significantly reduced in the group treated with a mixture of $16{\mu}g$ of mangosteen extract powder and $544{\mu}g$ of propolis extract powder (ligation [Lig] + lipopolysaccharide extracted from P. gingivalis KCOM 2804 [L] + MEC 1:34). Additionally, alveolar bone loss was significantly reduced in the Lig + L + MEC 1:34 group compared with that in other groups. These results indicate that the MEC could be useful in preventing and treating periodontitis.