Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Specificity Protein 1 Expression Contributes to Bcl-w-Induced Aggressiveness in Glioblastoma Multiforme

  • Lee, Woo Sang (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences) ;
  • Kwon, Junhye (Department of Biological Sciences, Sookmyung Women's University) ;
  • Yun, Dong Ho (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences) ;
  • Lee, Young Nam (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences) ;
  • Woo, Eun Young (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences) ;
  • Park, Myung-Jin (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences) ;
  • Lee, Jae-Seon (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences) ;
  • Han, Young-Hoon (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences) ;
  • Bae, In Hwa (Division of Radiation Cancer Research, Korea Institute of Radiological & Medical Sciences)
  • Received : 2013.05.28
  • Accepted : 2013.11.26
  • Published : 2014.01.31
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Abstract

We already had reported that Bcl-w promotes invasion or migration in gastric cancer cells and glioblastoma multiforme (GBM) by activating matrix metalloproteinase-2 (MMP-2) via specificity protein 1 (Sp1) or ${\beta}$-cateinin, respectively. High expression of Bcl-w also has been reported in GBM which is the most common malignant brain tumor and exhibits aggressive and invasive behavior. These reports propose that Bcl-w-induced signaling is strongly associated with aggressive characteristic of GBM. We demonstrated that Sp1 protein or mRNA expression is induced by Bcl-w using Western blotting or RT-PCR, respectively, and markedly elevated in high-grade glioma specimens compared with low-grade glioma tissues using tissue array. However, relationship between Bcl-w-related signaling and aggressive characteristic of GBM is poorly characterized. This study suggested that Bcl-w-induced Sp1 activation promoted expression of glioma stem-like cell markers, such as Musashi, Nanog, Oct4 and sox-2, as well as neurosphere formation and invasiveness, using western blotting, neurosphere formation assay, or invasion assay, culminating in their aggressive behavior. Therefore, Bcl-w-induced Sp1 activation is proposed as a putative marker for aggressiveness of GBM.

Keywords

References

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