International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Effect of Garcinia mangostana L. and propolis extracts on the inhibition of inflammation and alveolar bone loss in ligature-induced periodontitis in rats

  • Sung, Se-Jin (Department of Periodontology, School of Dentistry, Chosun University) ;
  • Kang, Kyung-Min (Department of Periodontology, School of Dentistry, Chosun University) ;
  • Lee, Kyung-Hyun (Department of Periodontology, School of Dentistry, Chosun University) ;
  • Yoo, So-Young (Medi Bio Lab Co., Ltd.) ;
  • Kook, Joong-Ki (Korean Collection for Oral Microbiology, School of Dentistry, Chosun University) ;
  • Lee, Dae Sung (Medi Bio Lab Co., Ltd.) ;
  • Yu, Sang-Joun (Department of Periodontology, School of Dentistry, Chosun University)
  • Received : 2019.06.04
  • Accepted : 2019.06.11
  • Published : 2019.06.30
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Abstract

The purpose of this study was to evaluate the effect of mangosteen extract complex (MEC; Garcinia mangostana L. and propolis extracts) on the inhibition of inflammation and prevention of alveolar bone loss using a ligature-induced periodontitis model. Rat molars were ligatured with silk, and $1{\mu}g/mL$ of lipopolysaccharide of Porphyromonas gingivalis was injected into the buccal and palatal gingivae of the teeth with or without treatment with the MEC. Changes in the expression levels of prostaglandin $E_2$ ($PGE_2$), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), matrix metalloproteinase-8 (MMP-8), cyclooxygenase (COX)-1, and COX-2 in gingival tissues were evaluated using enzyme-linked immunosorbent assays. Alveolar bone loss around the ligated molars was examined using micro-computed tomography. The expression levels of $PGE_2$, IL-8, iNOS, MMP-8, COX-1, and COX-2 in gingival tissues were significantly reduced in the group treated with a mixture of $16{\mu}g$ of mangosteen extract powder and $544{\mu}g$ of propolis extract powder (ligation [Lig] + lipopolysaccharide extracted from P. gingivalis KCOM 2804 [L] + MEC 1:34). Additionally, alveolar bone loss was significantly reduced in the Lig + L + MEC 1:34 group compared with that in other groups. These results indicate that the MEC could be useful in preventing and treating periodontitis.

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References

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