• Bulletin of the Korean Chemical Society
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• 제28권6호
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• pp.1005-1009
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• 2007

Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various Gα protein including Gα i1, Gα i2, Gα i3, Gα s1, Gα o1, and Gα z. Among the tested Gα subunits, Gα i3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (KD) of 5.0 × 10?6 M. These results suggest that Gα i3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with Gα i3.

• 한국자기학회:학술대회 개요집
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• 한국자기학회 1994년도 추계연구발표회 논문개요집
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• pp.30-31
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• 1994

• Bulletin of the Korean Chemical Society
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• 제26권12호
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• pp.2033-2037
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• 2005

In vitro selection was used to isolate $Mg^{2+}$-dependent self-cleaving ribozymes with cis-cleavage activity from a pre-tRNA library having 40-mer random sequences attached to 5'-end of E. coli $tRNA^{Phe}$. After 8 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment), RNA molecules which can self-cleave at the high concentration of $Mg^{2+}$ were isolated. The selected ribozymes can carry out the self-cleavage reaction in the presence of 100 mM $Mg^{2+}$ but not in 10 mM $Mg^{2+}$. The cleavage sites of the ribozymes are located at +3 and +4 of $tRNA^{Phe}$, compared with +1 position of 5'-end cleavage site of pre-tRNA by RNase P. New RNA constructs deprived of its D stem-loop, anticodon stem-loop, variable loop and T stem-loop, respectively showed the cleavage specificity identical to a ribozyme having the intact tRNA structure. Also, the new ribozyme fused with both a ribozyme and $tRNA^{Leu}$ showed the cleavage activities at the various sites within its sequences, different from two sites of position +3 and +4 observed in the ribozyme with $tRNA^{Phe}$. Our results suggest that the selected ribozyme is not structural-specific for tRNA.

• Nuclear Medicine and Molecular Imaging
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• 제43권4호
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• pp.337-343
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• 2009

목적: (R)-1-(2-Chlorophenyl)-N-methyl-N-1-(1-methyl-propyl)-3-isoquinoline carboxamide ((R)-PK11195)는 말초형 벤조디아제핀 수용체 (PBR)의 친화성이 높으며, 활성화된 소교세포(microglia)의 PBR에 선택적으로 결합하는 것으로 알려져 있다. 본 연구에서는 말초형 벤조디아제핀 수용체 (PBR)의 FET용 리간드인[$^{11}$C](R)-PK11195를, 절차가 빠르고 간단한 [$^{11}$C]CH$_3$I 자동합성장치 및 루프법을 도입해서 합성하였다. 대상 및 방법: 사이클로트론에서 $^{14}$N(p,a)$^{11}$C핵반응에 의하여 생산된 [$^{11}$C]Co$_2$를 0.2 M LiAlH$_4$/THF (0.2 mL)로 환원한 다음 HI (1 mL)과 반응하여 [$^{11}$C]CH$_3$I를 생산하였다. 반응용매인 NaH DMF complex (0.1 mL)와 녹인 전구물질 (R)-N-desmethyl-PK11195 (1 mg)을 녹인 DMSO (0.1mL)를 섞은 혼합액을 HPLC의 루프에 미리 주입하고 [$^{11}$C]CH$_3$I를 상온에서 5분 동안 질소가스로 불어준 뒤에 semi-preparative HPLC로 [$^{11}$C](R)-PK11195를 분리하였다. 결과: 전구물질과 [$^{11}$C]CH$_3$I의 [$^{11}$C]메틸화 반응에서의 표지효율은 71.8$\pm$8.5% 이었다. 분리 후 얻은 [$^{11}$C](R)-PK11195의 비방사능은 11.8$\pm$6.4 GBq/$\mu$mol이었으며, 방사화학적 순도는 99.2% 이상이었다. C-11 표지 후 얻어진 (R)-PK11195의 물질의 질량 분석은 m/z 353.1 (M+1)으로 물질구조를 확인 할 수 이었다. 결론: 뇌염증에 의한 활성화 소교세포의 영상화를 위한 PET용 방사성의약품인 [$^{11}$C](R)-PK11195를 반복적으로 생산해야 하는 임상 적용을 위해, 합성절차가 빠르고 간단한 [$^{11}$C]CH$_3$I 자동화 합성장치를 1차 루프 방법을 이용하여 $^{11}$C을 표지 할 수 있었다. 이 연구를 통해서 [$^{11}$C](R)PK11195 표지 할 때 반응 단계를 줄이고 반응을 실온에서 할 수 있어서 표지과정을 보다 단순화할 수 있었으며, NaH의 DMF현탁액을 사용함으로써 보다 안전하게 생산할 수 있게 되었다.

• 한국자동차공학회논문집
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• 제9권6호
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• pp.186-194
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• 2001

To meet the challenge of testing increasingly complex automotive control systems, the real-time hardware-in-the-loop(HIL) simulation technology has been developed. In this paper, a strategy for evaluation of semiactive suspension systems using real-time HIL simulation is presented. A multibody vehicle model is adopted to simulate vehicle dynamic motions accurately. Accuracy of the vehicle simulation results is compared to that of the real vehicle field test and proven to be very accurate. The controller and stepping motor to adjust semi-active damper stage are equipped as external hardwares and connected to the real-time computer which has vehicle dynamic model. Open and closed loop test methods are used to evaluate a controlled suspension system and the system's operations are verified it is found that the proposed evaluation methods can be used well for the verification of semi-active suspension systems.

• 한국분무공학회지
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• 제8권1호
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• pp.23-28
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• 2003

When a cold HPSI (High Pressure Safety Injection) fluid associated with a design basis accident, such as LOCA (Loss of Coolant Accident), enters the cold legs of a stagnated primary coolant loop, thermal stratification phenomena may arise due to incomplete mixing. If the stratified flow enters a reactor pressure vessel downcomer, severe thermal stresses are created in a radiation embrittled vessel wall by local overcooling. Previous thermal-mixing analyses have assumed that the thermal stratification phenomena generated in stagnated loop of a partially stagnated collant loop are neutralized in the vessel downcomer by strong flow from unstagnated loop. On the basis of these reasons, this paper presents the thermal-mixing analysis results in order to identify the fact that the cold plume generated in the vessel downcomer due to the thermal stratification phenomena of the stagnated loop is affected by the strong flow of the unstagnated loop.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2130-2135
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• 2007

Operating characteristics of a loop heat pipe (LHP) having a bypass line was investigated experimentally. The LHP had a sintered metal wick as a capillary structure and methanol as a working fluid. The sintered metal wick was made of stainless steel of which the average pore size was 5 ${\mu}m$and porosity of 47%. A bypass line of a small diameter was attached between the vapor escape passage and the liquid reservoir. The dimension of the flat evaporator was $50(L){\times}40(W){\times}30(H)$ mm and that of the condenser was $50(L){\times}40(W){\times}11(H)$ mm. Wall and pipe material of the LHP was stainless steel and heating area was 35(W) mm${\times}$35(L) mm. The inner diameters of vapor and liquid transport lines were 4.0 mm and 2.0 mm, and the lengths of the two lines were both 0.5 m. The LHP was tested for three different tilt angles of horizontal, favorite tilt, and adverse tilt. The thermal load range was up to 290 W at the condenser above evaporation position. Furthermore, the effect of a bypass line on the start-up transient as well as steady-state operation was presented and discussed.

• Journal of Magnetics
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• 제1권2호
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• pp.69-74
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• 1996

Justeresos loops of melt-spun Nd13Fe77B10 cooled down at remanent state were measured at 4.2 K and 250 K. The hysteresis loops were analysed on the basis of the Stoner-Wohlfarth (S-W) model, the inter-grain exchange coupled single domain (SD) model and micromagnetism. The coercivity higher than that predicted from the S-W model and the striking shift of the thin minor loop along the H-axix observed at the fields of Hmax=4MA/m at 4.2 K indicated new evidences for the inter-grain exchange interaction . The S-W model failed in explaining the high iHc and the shift of the thin minor loop. The exchange coupled SD model was found to explain the experimental results qualitatively without difficulties associated with the S-W model. The micromagnetic calculations using a finite element technique simulated the experimental results fairly well quntitatively.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 춘계학술대회
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• pp.905-910
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• 2004

Vibration characteristics of the FIV test loop for the Flow-Induced Vibration(FIV) study of a PWR partial(5x5) fuel assembly are investigated by the Finite Element(FE) analysis and the modal test. For the FE analysis, 3-D beam element is used for the pipes and the test section and mass element used for the valves and flanges. The 'U' restrainer stiffness determined by numerical simulation is used for the FE model. The result of the FE analysis is compared with that of the modal test. The higher mode similarity between the test and analysis is observed in a few low modes. After that, the mode similarity reduce as the mode goes high. It is concluded that the first to the third vibration modes are observed at the lower parts of the 6 inches restoring line, followed by a local mode at the test section, and the natural frequencies of the modes are 22.4 Hz, 26.0 Hz, 27.5 Hz and 31.4 Hz.

• Progress in Superconductivity
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• 제6권1호
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• pp.84-88
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• 2004

The polycrystalline superconductor $SmBa_2$$Cu_3$$O_{x}$ is fabricated, and intergranular magnetic properties are investigated using the critical state model, from which some useful parameters such as the critical current density and the intergranular volume fraction are obtained. The curve fitting for M-H hysteresis loop shows that the intergranular critical current density of $SmBa_2$$Cu_3$$O_{x}$ / decreases in the form of ($1-T/T_{c}$ )$^{1.5}$ . The intergranular volume fraction is influenced by granular morphology. From SEM image, the grains of $SmBa_2$$Cu_3$$O_{x}$ are found to be randomly shaped. This mean:; that the intergranular volume fraction of $SmBa_2$$Cu_3$$O_{x}$ / should be smaller than those of superconductors, of which grains are plate-shaped such as Tl-based superconductor.