Cotton Glutathione S-Transferase (GST: EC 2.5.1.18) was cloned and overexpressed in tobacco (Nicotiana tabacum) plants. Northern blot analysis confirmed the successful transformation of cotton gst gene in tobacco plant. Type I and Type ll transcript patterns were identified in transgenic tobacco plants and only Type I transcripts were discussed in this paper, The activity of GST in the type II transgenic plants was about 1.5-fold higher than those of the wild type and non-expresser by using 1-chloro-2,4-dinitrobenzene (CDNB) and reduced glutathione as the substrate. The expression of cotton GST in tobacco plants proved that Gh-5 could be translated into functional protein. Type II transgenic plants produced functional GST in the cells. The effects of cotton GST in the seedlings was evaluated by growing the control and transgenic seedlings at $15^{\circ}C$ in the growth chamber in the light. Overexpressors were grown well compared to the control plants (non-expressors). lo test far tolerance to salinity, seeds of Gh-5 overexpressors and the wild type Xanthi seedlings were grown at 0, 50, 100, 150, and 200 mM NaCl solution. Gh-5 transgenic seedlings showed higher growth rate over control seedlings on 50 and 100 mM NaCl solution. There was no difference in growth rate at 150 and 200mM NaCl concentration.
Journal of Dental Rehabilitation and Applied Science
/
v.16
no.2
/
pp.113-122
/
2000
The reinforced composte resin as the esthetic operative material continuously has been studied because the porcelain fused metal prosthesis is widely used for its excellent esthetics, rigidity and marginal integrity, but it has low fracture resistance against the tensile strength and stress, attrition of the opposite teeth. The reinforced composite resin is well adapt with the dental alloy but it is low the shear bond strength with the dental alloy vs the porcelain fused metal prosthesis, and then has been studied continuously. The purpose of the study was to examine how metal was the higher shear bond strength among the dental alloy was used to the reinforced composite resin and to find the effect that the particle size of sandblasting influenced the shear bond strength. We built up the reinforced composite resin with 4 mm in diameter, 3 mm in height on circular alloy with 5 mm in diameter, 2 mm in height. Type II gold, type IV gold, and Ag-Pd alloy was used as alloys and $50{\mu}m$, $110{\mu}m$, $250{\mu}m$ of the particle size was sandblasted at each alloy in bonding between alloy and resin. We made 90 secimens of 10 per each group and we measured the shear bond strength using the Instron($M100EC^{(R)}$, Mecmesin Co., England). The obtained results were as follows : 1. In comparison among each alloys, Ag-Pd alloy had the highest shear bond strength and the shear bond strength was decreased significantly in the sequence of the type II gold and type IV gold(P<0.001). 2. In comparison according to the size of sandblasting particle, (1) In Ag-Pd alloy, shear bond strength was decreased in the sequence of $110{\mu}m$, $250{\mu}m$, $50{\mu}m$ and there were significant difference in all the group. (P<0.05) (2) In type II gold, it was decreased in the sequence of $250{\mu}m$, $50{\mu}m$, $110{\mu}m$ and there were significant difference. (P<0.05) (3) In type IV gold, it was decreased in the sequence of $110{\mu}m$, $50{\mu}m$, $250{\mu}m$. There were significant difference between the group of $110{\mu}m$ and $50{\mu}m$, the group of $110{\mu}m$ and 250, but there were no significant difference in the group of $50{\mu}m$ and $250{\mu}m$. 3. The highest shear bond strength according to the size of sandblasting particle was $110{\mu}m$ in Ag-Pd alloy and type IV gold, $250{\mu}m$ in type II gold.
Journal of the Korean Institute of Electrical and Electronic Material Engineers
/
v.28
no.8
/
pp.518-523
/
2015
We fabricate a single particle-microcapsule type electronic paper using electrophoresis, which is different with a reported dual particle-microcapsule type and of which electro-optical researches are not reported. So we analyzed a basic properties, such as reflectivity, response time, and driving voltage. Our display panels having various cell-gaps of $30{\mu}m$, $34{\mu}m$, $38{\mu}m$, $42{\mu}m$, and $46{\mu}m$ are inspected. As a results, a driving voltage is defined to 10 V and desirable cell-gap is $30{\mu}m$ or $34{\mu}m$. Considering a mechanical strength, the optimum cell-gap is $34{\mu}m$ for the single particle type electronic paper.
Journal of the Korean Society of Fisheries and Ocean Technology
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v.39
no.3
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pp.197-210
/
2003
The non-float midwater pair trawl was effective in the mouth opening and control of the working depth in midwater and bottom. In contrast, we confirmed that it was difficult to keep the net at surface above 30 m of the depth by means of the full scale experiment in the field and the model test in the circulation water channel. To solve this problem, the kites were attached to the head rope of the non-float midwater pair trawl. In this study, four kinds of the model experiments were carried out with the purpose of applying the kite to the korean midwater pair trawl. The results obtained can be summarized as follows: 1. The working depth of the non-float midwater pair trawl with the kite was shallower than that of the proto type and non-float type. The working depth of the kite type was approximately 20m with 2 kites and about 5m with 4 kites under 4.0 knot. The working depth was almost constant but the depth of the head rope sank approximately 15m and 10m according to the increase in the front weight and the wing-end weight, respectively. The changing aspect of the working depth was constant, but the depth of the head rope sank approximately 22m according to the increase in the lower warp length (dL). 2. The hydrodynamic resistance of the kite type was almost increased in a linear form in accordance with the flow speed increase from 2.0 to 5.0 knot. The increasing grate of the hydrodynamic resistance tended to increase in accordance with the increase in flow speed. The hydrodynamic resistance of the kite type was larger approximately 5~10 ton larger than that of the non-float type and the proto type. The hydrodynamic resistance of the kite type increased approximately 3ton with the changing of the front weight from 1.40 to 3.50 ton and approximately 4 ton with the changing of the wing-end weight from 0 to 1.11 ton and approximately 5.5 ton with the changing lower warp length (dL) from 0 to 40 m, respectively. 3. The net height of the kite type was increased approximately 10 m with the change in the kite area from $2,270mm^2$ to 4,540 $\textrm{mm}^2$. The net height of the kite type was aproximately 50 m and 30 m larger than that of the proto type and the non-float type, respectively. The changed aspect of the net width was approximately 5m with the variation of the flow speed from 2.0 to 5.0 knot. 4. The filtering volume of the kite type was larger than that of the proto type and the non-float type by 28%, 34% at 2.0 knot of the flow speed and 42%, 41% at 3.0 knot, and 62%, 45% at 4.0 knot, and 74%, 54% at 5.0knot, respectively. The optimal towing speed was approximately 3.0 knot for the proto type and was over 4.0 knot for the non-float type, and the optimal towing speed reached 5.0 knot for the kite type. 5. The opening efficiency of the kite type was approximately 50% and 25% larger than that of the proto type and the non-float type, respectively.
In the Cheongju-Minwon area which occupies the middle part of the Ogcheon Metamorphic Belt, three metamorphic events(M1, M2, M3) had occurred. Intermediate P/T type M2 regional metamorphism formed prevailing mineral assemblages in the study area. Low PIT type M3 contact metamorphism occurred due to the intrusion of granites after M2 metamorphism. M1 metamorphism is recognized by inclusions within garnet. During M2 metamorphism, the metamorphic grade increased from the biotite zone in the southeastern part to the garnet zone in the northwestern part of the study area. This result is similar to the metamorphic evolution of the southwestern part of the Ogcheon Metamorphic Belt. Garnets in the garnet zone are classified into two types; Type A garnet has inclusions whose trail is connected to the foliation in the matrix and Type B garnet has inclusion rich core and inclusion poor rim. Type A garnet formed in the mica rich part with crenulation cleavage whereas Type B garnet formed in the quartz rich part with weak crenulation cleavage. In some outcrops, two types garnets are found together. Compared to the rim of Type A garnet, the rim of Type B garnet is lower in grossular and spessartine contents but higher in almandine and pyrope contents. In some Type B garnets, the inclusion poor part is rimmed by muddy colored or protuberant new overgrowth. In the inclusion poor part and new overgrowth, a rapid increase in grossular and decrease in spessartine is observed. However, the compositional patterns of Type A and B are similar; Ca increases and Mn decreases from core to rim. Two types garnets formed mainly due to the difference of bulk chemistry instead of metamorphic and deformational differences. The metamorphic P-T conditions estimated from Type A garnets are 595-690 OC15.7-8.8 kb, which indicates M2 metamorphism is intermediate P/T type metamorphism. On the other hand, a wide range of P-T conditions is calculated from Type B garnets. The P-T conditions from most Type B garnet rims are 617-690 OC16.2-8.9 kb which also indicates an intermediate P/T type metamorphism. However, at the rim part with flat end or weak overgrowth, grossular content is low and 573-624OC14.7-5.8 kb are estimated. The P-T conditions calculated from plagioclase and biotite inclusions in garnet are 460-500 0C/1.9-3.0 kb. The P-T conditions from rim part with weak overgrowth and inclusions within garnet, indicate that low P/T type M1 regional metamorphism might have occurred before intermediate P/T type M2 regional metamorphism. The P-T conditions estimated from samples which had undergone low PIT type M3 metamorphism strongly, are 547-610 0C/2.1-5.0 kb.
The change in vegetation structure along elevational gradients on the southeastern part of Mt. Hallasan was studied. Vegetation data were collected with 59 quadrates located from 16 to 1,565 m with 100 m intervals. Community types were classified using cluster analysis, and species composition and diversity were analyzed along elevational gradients. The vegetation was classified into seven, namely, type 1 Quercus serrata community, type 2 Carpinus tschonoskii community, type 3 Carpinus laxiflora community, type 4 Pinus densiflora community, type 5 Abies koreana community, type 6 Castanopsis sieboldii community, and type 7 Quercus acuta community. The species with a high importance value in tree layer in each elevational zone were C. sieboldii and Q. acuta at 100-600 m; C. laxiflora, Q. serrata, and C. tschonoskii at 700-800 m; P. densiflora at 1,100-1,200 m; and Abies koreana at 1,500-1,600 m. The species diversity indicated higher value at 700-800 m, 1,200-1,300 m and 1,400-1,500 m than at the other elevation.
In order to develop facility for complex farming with aquaculture and vegetable nutrient culture and to investigate growth of tilapia and water quality in the channel type aquaculture system, these experiments were carried out. When tilapia(Tilapia nilotica) was reared in the channel type aquaculture system, Quality of culture water and growth of tilapia were normal. And the growth of tilapia in the channel type and round type aquaculture system was much the same. Channel type aquaculture facilities was applicable to the dual culture system for aquaculture and vegetable culture. Channel type integrated system was composed of aquaculture and sandculture bed, and it was thought that suitable size of the system was about 1.4m(width) $\times$ 1m(height) $\times$ 20m(length).
The author observed the positional relationship of maxillary cuspid to ala-lacrimal duct line in the 594 Koreans(329 male, 265 female) who had relatively normal teeth arrangement, aged 16 to 70. The relationship of ala-lacrimal duct line to the distal angie of maxilly cuspid of the subjects were classified as follows; Type A : Ala-lacrimal duct line was lotated more medially than the distal angle of maxillary cuspid. Type B : Ala-lacrimal duct line coincided with the distal angle of maxillary cuspid. Type C: Ala-lacrimal duct line was located more distally than the distal angle of maxillary cuspid. The occlusal relationship of the maxillary first molar to the mandibular first molar of the subjects were classified as follows; Group I : Group of neutrocclusion relationship. Group II : Group of distocclusion relationship. Group III : Group of mesiocclusion relationship. The obtained results were as follows; 1) The positional relationship of maxillary cuspid to ala-lacrimal duct line were as follows; (Male) Type A: $68.5%{\pm}1.58m%$, Type B: $22.5%{\pm}2.30m%$, Type C: $9.1%{\pm}\1.58m%$ (Female) Type A : $64.5%{\pm}2.94m%$, Type B: $26.5%{\pm}2.71m%$, TypeC: $8.9%{\pm}1.73m%$ 2) Ala-lacrimal duct line located more distally in group II and group III than group I at the point of the positional relationship of upper and lower first molar. 3) Average distance and standard deviation in type A were as follows; (Male) Right: $2.17mm.{\pm}1.01mm.$ Left: $2.27mm.{\pm}1.02mm.$ (Female) Right: $2.06mm.{\pm}0.70mm.$ Left: $2.24mm.{\pm}0.80mm.$ 4) Average distance and standard deviation in type C were as follows; (Male), Right: $1.47mm.{\pm}0.85mm.$ Left: $1.52mm.{\pm}0.97mm.$ (Female) Right: $1.04mm.{\pm}0.44mm.$ Left: $1.26mm.{\pm}0.89mm.$ The results of 3) and 4) had no significant difference between the male and female.
Purpose: To investigate the effects of irradiation on the phenotypic expression of the MC3T3-El osteoblastic cell line, particularly an the expression of type I collagen and alkaline phosphatase mRNA. Materials and Methods: Cells were irradiated with a single dose of 0.5, 1, 2, 4, and 8 Gy at a dose rate of 5.38 Gy/min using a cesium 137 irradiator. The specimens were then harvested and RNA extraction was carried out at 1 and 3 days after irradiation. The extracted RNA strands were reverse-transcribed and the resulting cDNA fragments were amplified by PCR. Results: The irradiated cells demonstrated a dose-dependent increase in type I collagen mRNA expression relative to the control group, with a maximum level of type I collagen mRNA expression occurring at 8 Gy. The degree of type I collagen mRNA expression increased significantly at 1 day after irradiation, but little differences were found between the control group and at the 3rd day. The amount of alkaline phosphatase mRNA expression increased significantly at land 3 days after irradiation in the 1 Gy exposed group compared with the control group. Conclusion: The amount of type I collagen and alkaline phosphatase mRNA expression increased significantly 1 day after irradiation when compared with the control group.
General consensus on typical vertical profile of dissolved oxygen in the Ulleung Basin is that dissolved oxygen concentration beyond 300 m decreases with increasing depth. However, the results of our observations in 2005 and 2006 revealed three different dissolved oxygen distribution types in the deep layer of the Ulleung Basin. The first type showed oxygen concentration decreasing with increasing depth (Type-1), the second showed oxygen concentration decreasing very sharply near the bottom boundary layer but constant in the bottom adiabatic layer (Type-2), the final was of the oxygen minimum layer above the bottom boundary layer (Type-3). Type-2 was the most common pattern in the Ulleung Basin. Type-1 was most common close to the Japan Basin, including the Ulleung Interplane Gap, while Type-3 was found around Dok do. Oxygen Consumption Rate (OCR) at surface sediment estimated using the dissolved oxygen distribution at the bottom boundary layer was $0.2{\sim}5.8\;mmol{\cdot}m^{-2}d^{-1}$, which coincided with OCR from direct sediment incubation. This implies that organic matter decomposition at surface sediment may play an important role in dissolved oxygen distribution patterns at the bottom boundary layer of the Ulleung Basin.
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