• Journal of Acupuncture Research
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• 제19권2호
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• pp.65-77
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• 2002

Objective : This study was undertaken to determine which compound of Bee Venom for herb-acupuncture has cytotoxicity on mouse mast cell line. Methods : We compared crude bee venom and its compounds such as melittin, mast cell degranulating peptide (MCD peptide), apamin with control groups on cytotoxicity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results & Conclusion : 1. Crude bee venom showed significant cytotoxic effect(p<0.01) in 1 hour treatment with $1{\mu}g/m{\ell}$ in comparison with control group in 1 hour treatment with low concentration of $10-4{\mu}g/m{\ell}$, $10-3{\mu}g/m{\ell}$, $10-2{\mu}g/m{\ell}$, $10-1{\mu}g/m{\ell}$ and $1{\mu}g/m{\ell}$, but it showed no significant cytotoxic effect in 6 hours treatment. 2. Melittin group showed no significant cytotoxic effect in comparison with control group in 1 and 6 hours treatment with low concentration of $10-4{\mu}g/m{\ell}$, $10-3{\mu}g/m{\ell}$, $10-2{\mu}g/m{\ell}$, $10-1{\mu}g/m{\ell}$ and $1{\mu}g/m{\ell}$. 3. MCD peptide and Apamin group showed no significant cytotoxic effect in comparison with control group in 1 and 6 hours treatment with low concentration of $10-4{\mu}g/m{\ell}$ $10-3{\mu}g/m{\ell}$, $10-2{\mu}g/m{\ell}$, $10-1{\mu}g/m{\ell}$ and $1{\mu}g/m{\ell}$. 4. Crude bee venom showed significant cytotoxic effect(p<0.01) in 1 and 6 hours treatment in comparison with control group in 1 and 6 hours treatment with high concentration of $10{\mu}g/m{\ell}$, $20{\mu}g/m{\ell}$ and $102{\mu}g/m{\ell}$. 5. Melittin group showed significant cytotoxic effect(p<0.01) in 1 hour treatment in comparison with control group in 1 hour treatment with high concentration of $10{\mu}g/m{\ell}$ but it showed no significant cytotoxic effect in 6 hours treatment. 6. Crude bee venom and its compounds have more cytotoxic effect in 1 hour treatment than in 6 hours treatment. It means cytotoxicity tends to decrease according to the treatment time.

• 한국산림과학회지
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• 제98권4호
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• pp.363-369
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• 2009

생물반응기를 이용하여 섬오갈피 부정근 증식시 부정근의 생장과 유용 이차대사산물인 eleutheroside 류 생산에 미치는 ${NO_3}^-$와 ${NH_4}^+$의 영향 및 배양기간에 따른 배지성분의 변화량을 분석하였다. 부정근 증식은 질소농도가 50 mM ${NO_3}^-$와 10 mM ${NH_4}^+$ 농도비율로 첨가된 처리구에서 최대(30.8 g FW/L)에 이르러 60 mM ${NH_4}^+$ 첨가에 비해서는 3.6배 증식되었다. 또한 ${NH_4}^+$의 첨가농도비율이 높아질수록 부정근의 증식은 감소하였다. 그러나 eleutheroside B는 총 질소량이 30 mM 처리에서 $57.3{\mu}g/g$ DW, E는 60 mM 처리에서 $188.4{\mu}g/g$ DW의 함량으로 생산량이 가장 많았다. 반면에 eleutheroside $E_1$은 총 질소량이 15 mM 처리에서 $47.3{\mu}g/g$ DW로 생산량이 가장 많았다. 부정근 최대생장은 초기 접종량 대비 배양 9주만에 6.8배가 증식되었고, 배양기간별 배지 성분의 변화에서 ${NH_4}^+$, $K^+$, ${NO_3}^-$와 ${PO_4}^-$는 배양기간이 경과할수록 함유량이 감소하였다. 이러한 결과로 보아 부정근의 생장과 유용이차대사산물의 함량을 높이기 위하여 다양한 처리의 연구가 수행되어야 할 것으로 생각된다.

• Journal of Communications and Networks
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• 제3권4호
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• pp.361-364
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• 2001

Over an additive abelian group G of order g and for a given positive integer $\lambda$, a generalized Hadamard matrix GH(g, $\lambda$) is defined as a gλ$\times$gλ matrix[h(i, j)], where 1 $\leq i \leqg\lambda and 1 \leqj \leqg\lambda$, such that every element of G appears exactly $\lambd$atimes in the list h($i_1, 1) -h(i_2, 1), h(i_1, 2)-h(i_2, 2), …, h(i_1, g\lambda) -h(i_2, g\lambda), for any i_1\neqi_2$. In this paper, we propose a new method of expanding a GH(g^m, \lambda_1) = B = [B_{ij}] over G^m$ by replacing each of its m-tuple B_{ij} with B_{ij} + GH(g, $\lambda_2) where m = g\lambda_2. We may use g^m/\lambda_1 (not necessarily all distinct) GH(g, \lambda_2$)s for the substitution and the resulting matrix is defined over the group of order g.

• 대한소아치과학회지
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• 제8권1호
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• pp.77-88
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• 1981

The purpose of this study was to make a comprehensive study & evaluation of the oral status of mental retarded children. The auther examined intraorally 486 (male; 311, female;175) mental retarded children. The result was as follows; (General mental retarded children means the children who live in their parent's home, & orphan mental retarded children means the children who live in orphanage.) 1. The dft rate was 31.6% in general mental retarded children (G.m.r.c.) & 20.7% in orphan mental retarded children (O. m. r. c.). The dft index was 3.73 in G.m.r.c. & 2.15 in O.m.r.c. 2. The DMFT rate was 24.6% in female G.m.r.c., 16.7% in male G.m.r.c., 12.7% in female O.m.r.c., 8.4% in male O.m.r.c. The DMFT index was 4.94 in female G.m.r.c., 4.01 in male G.m.r.c., 1.40 in male O.m.r.c., 2.75 in female O.m.r.c. 3. The malocclusion prevalence was 57.3%. the class I malocclusion was 14.2% Class II malocclusion 19.3%, Class III malocclusion 23.5%. The children with Down's syndrome had 60.0% of class III malocclusion prevalence. 4. The dental calculus index was 1.97 in male O.m.r.c., 1.81 in female O.m.r.c., 1.30 in male G.m.r.e., 1.13 in female G.m.r.c. 5. The dental plaque index was 3.06 in female G.m.r.c., 3.00 in male Gm.r.e. 2.70 in male O.m.r,c., 2.32 in female O.m.r.c.

• Biomolecules & Therapeutics
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• 제6권2호
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• pp.111-118
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• 1998

Ambroxol is thought to have antioxidant ability and some antiinflammatory effect. Effect of ambroxol on the oxidative damages of lipid, collagen and hyaluronic acid was examined. F $e_{2+}$(10 $\mu$M) and 100$\mu$Mascorbate-induced lipid peroxidation of liver microsomes was inhibited by 10 and 100$\mu$M ambroxol, 30$\mu$g/ml catalase and 10 mM DABCO but was not affected by 30$\mu$g/ml SOD and 10 mM DMSO. A 10 and 100$\mu$M ambroxol and 10 mM DABCO inhibited the peroxidative action of 10$\mu$M F $e_{3+}$, 160$\mu$M ADP and 100$\mu$M NADPH on microsomal lipids, whereas inhibitory effects of 30$\mu$g/ml SOD,30$\mu$g/ml catalase and 10 mM DMSO were not detected. The degradation of hyaluronic acid caused by 107M Fe2\\`,5007M H2O2 and 100$\mu$M ascorbate was inhibited by 10 and 100$\mu$M ambroxol,30$\mu$g/ml catalase,10 mM DMSO and 10 mM DABCO, while 30$\mu$g/ml SOD did not show any effect. The cartilage collagen degradation caused by 307$\mu$ F $e_{2+}$,500$\mu$M $H_2O$$_2$ and 200$\mu$M ascorbate was prevented by 100$\mu$M ambroxol. $H_2O$$_2$ and OH . were scavenged by ambroxol, whereas $O_2$, was not removed by it. Ambroxol (100$\mu$M) and 1 mM cysteine reduced DPPH to 1,1-diphenyl-2-picrylhydrazine. In conclusion, ambroxol may inhibit the oxidative damages of lipid, hyaluronic acid and collagen by its scavenging action on oxidants, such as OH . and probably iron-oxygen complexes and exert antioxidant ability.

• 한국식품영양학회지
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• 제23권3호
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• pp.306-310
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• 2010

This study was designed to investigate the protective effect of the combination of fucoidan and lutein against AAPH-induced oxidative stress in THP-1 cells. The combination of fucoidan and lutein existed significant antioxidant effect on AAPH-damaged THP-1 cells by using lipid peroxidation and cellular antioxidant capacity assay. Fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) did not affect at all the viability of THP-1 cells, but protected the AAPH-damage of THP-1 cells at the same concentration. The viability of THP-1 cells was 0% with 1 mM AAPH alone, the protective effect of fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) was 37% and 36%, respectively. The combination of fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) exhibited significant inhibitory effect of lipid peroxidation using TBARS assay and cellular antioxidant capacity using DCFH-DA assay. In lipid peroxidation, the TBARS value of 1 mM AAPH alone was $0.8{\pm}0.03\;nM$ MDA, its of the combination of fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) was $0.2{\pm}0.05\;nM$ MDA. In cellular antioxidant capacity, the combination of fucoidan($1\;{\mu}g/m{\ell}$) and lutein($10\;{\mu}g/m{\ell}$) exhibited significant cellular antioxidant capacity of 76%, whereas quercetin($10\;{\mu}M$) as positive control exhibited the cellular antioxidant capacity of 32%. These results indicate that the cotreatment of fucoidan and lutein protects against AAPH-induced THP-1 cell damage by inhibiting lipid peroxidation, increasing cellular antioxidant capacity.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 춘계학술대회
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• pp.352-357
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• 2003

Creep tests for Titan were carned out using constant-load at $600^{\circ}C$, $650^{\circ}C$ and $700^{\circ}C$. Material constants necessary to predict creep life were acquired from the experimental creep data. And the applicability of Monkman-Grant(M-G) and modified M-G relationships was discussed. It was discovered the log-log plot of M-G relationships between the rupure time(tr) and he minimum creep rate(${\varepsilon}_m$) was conditional on test temperatures. The slop of m was 2.75 at $600^{\circ}C$ and m was 1.92 at $700^{\circ}C$. However; the log-log plot of modified M-G relationships between $t_r/\varepsilon_r$ and $\varepsilon_m$ was indpendent on stresses and temperatures. That is the slop of m' was almost 3.90 in all the data. Thus, change M-G relationships to creep life prediction could be vtilized more reasonably than that of M-G relationships for type Titan. It was divided that the constant slopes never theless of temperatures of practical stresses in the modified relationship were due to an intergranular break grown by wedge-type cauities.

• 대한약리학회지
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• 제9권2호
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• pp.1-6
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• 1973

Although numerous drugs are available for the treatment of superficial fungi infections of skin, the clinical effects of the majority of such drugs are not satisfactory. In the hope of searching the effective drugs for superficial fungi infections, authors studied whether Veratrum rhizoma extracts had any effect on fungi, with water extract (VRWE), ethanol extract (VREE) and methanol extract (VRME) from Veratrum album L. var. grandiflorum Max. In in vitro studies, the spores of fungi were inoculated on Sabouraud's glucose agar media which contained three extracts of Veratrun rhizoma in each concentration of $500\;{\mu}g/ml$, $1,000\;{\mu}g/ml$ and $5,000\;{\mu}g/ml$ respectively, and the growth of the fungi were observed for 3 weeks. The species of the fungi used in these experiments were Epidermophyton floccosum, Microsporum canis, Microsporum nanum, Microsporum gypseum, Microsporum cookei, Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton tonsurans and Trichophyton verrucosum. The results of the studies were as follows: 1. The growth of M. canis, M. nanum, T mentagrophytes and T. tonsurans were slightly inhibited by VRWE $1,000\;{\mu}g/ml$, and with VRWE $5,000\;{\mu}g/ml$, the growth of E. floccosum, M. gypseum and T. rubrum were slightly inhibited, moderate inhibition on the growth of M. canis, M. nanum, M. cookei, T. mentagrophytes and T. tonsurans were showed by VRWE $5,000\;{\mu}g/ml$. 2. With $500\;{\mu}g/ml$ of VREE, the inhibition on growth of E. floccosum, M. nanum and M. gypseum were slight, however significant inhibition on the growth of M. canis, M. cookei, T. mentagrophytes, T. rubrum and T. tonsurans were observed. The growth of M. nanum and M. gypseum were moderately inhibited, and significant inhibition on the growth of E. floccosum, M. canis, M. cookei, T. mentagrophytes, T. rubrum and T. tonsurans were observed by VREE $1,000\;{\mu}g/ml$. By VREE $5,000\;{\mu}g/ml$, the growth of all tested fungi were significantly inhibited except T. verrucosuia being showed slight inhibition. 3. Significant inhibition on the growth of M. canis, T, mentagrophytes, T. rubrum and T. tonsurans were noted, and moderate inhibition of M. nanum, slight inhibition of E. floccosum and M. gypseum in growth were observed by VRME $500\;{\mu}g/ml$. The growth of E. floccosum, M. canis, M. nanum, M. cookei, T. mentsgrophytes, T. rubrum and T. tonsurans were significantly inhibited by VRME $1,000\;{\mu}g/ml$, and that of M. gypseum was moderate. With $5,000\;{\mu}g/ml$ of VRME, significant inhibition on the growth of E. floccosum, M. canis, M. nanum, M. gypseum, M. cookei, T mentagrophytes, T. rubrum and T. tonsurans were observed, and T. verrucosum was showed only slight inhibition. From the above results, it was found that the extracts of organic solvents from Veratrum rhizoma (VREE & VRME) exerted significant antifungal activity, and their effects were probably derived from the pharmacological action of steroidal alkaloids.

• 한국물환경학회지
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• 제28권2호
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• pp.277-284
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• 2012

This study developed a new ceramics in which natural zeolite was mixed and calcined with industrial by-product such as converter slag, red mud, and fly ash and evaluated the feasibility of the ceramics for removal of heavy metals in acid wastewater. The removal rate of heavy metal by ceramics increased in the order of ZS (zeolite and slag) > ZR (zeolite and red mud) > ZF (zeolite and fly ash) ceramics. The alkalinity increment and coherence of ceramics were increased in the order of ZS > ZR > ZF ceramics. The mixing ratio of natural zeolite to industrial by-product for maximum removal efficiency of heavy metal was 1:1 for ZS ceramics and 1:3 for ZR and ZF ceramics. The order of removal efficiency of heavy metal was observed to be ZS > ZR > ZF ceramics under the mixing ratio of 1:1 for ZS ceramics and 1:3 for ZR and ZF ceramics. The removal efficiency of heavy metal by ZS ceramics with 1:1 mixing ratio was Al 100%, Cd 54.6%, Cr 99.9%, Cu 98.7%, Fe 99.9%, Mn 42.2%, Ni 59.9%, Pb 99.8%, Zn 87.6%, respectively. In addition, the removal capacity of heavy metal by ZS ceramics was observed to be Al 2.01 mM/g, Cd 0.27 mM/g, Cr 1.02 mM/g, Cu 0.83 mM/g, Fe 0.95 mM/g, Mn 0.41 mM/g, Ni 0.55 mM/g, Pb 0.25 mM/g, Zn 0.70 mM/g, respectively. The comparative evaluation in the light of removal capacity, alkalinity increment, and coherence of ceramics showed the ZS ceramics had higher feasibility as a media than others for removal of heavy metals in acid wastewater.

• 한국정보과학회논문지:시스템및이론
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• 제32권8호
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• pp.426-431
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• 2005

그래프 G의 다대다 k-서로소인 경로 커버(k-DPC)는 k개의 서로 다른 소스 정점과 싱크 정점 쌍을 연결하며 그래프에 있는 모든 정점을 지나는 k개의 서로소인 경로 집합을 말한다. 이 논문에서는 이중 루프 네트워크 G(mn;1,m)에서 다대다 2-DPC를 고찰하여, 이분 그래프가 아닌 모든 G(mn;l,m), $m{\geq}3$은 임의의 두 소스-싱크 쌍을 연결하는 다대다 2-DPC가 존재하고 이분 그래프인 G(mn;1,m)은 두 흰색-검정 소스-싱크 쌍이거나 혹은 검정-검정, 흰색-흰색 쌍을 연결하는 2-DPC가 존재함을 보인다. G(mn;1,m)은 m이 홀수이고 n이 짝수일 경우에만 이분 그래프이다.