Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Effect of Nitrogen on Eleutheroside production and Adventitious Root Growth in Eleutherococcus koreanum Nakai Bioreactor Cultures

생물반응기를 이용한 섬오갈피나무의 부정근 배양시 질소농도 및 NH4+와 NO3- 비율이 부정근의 생육과 eleutherosides 함량에 미치는 영향

  • Ahn, Jin-Kwon (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Lee, Wi-Young (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Park, Eung-Jun (Department of Forest Genetic Resources, Korea Forest Research Institute)
  • 안진권 (국립산림과학원 산림유전자원부) ;
  • 이위영 (국립산림과학원 산림유전자원부) ;
  • 박응준 (국립산림과학원 산림유전자원부)
  • Received : 2009.03.04
  • Accepted : 2009.04.27
  • Published : 2009.09.30
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Abstract

This study was carried out to investigate the effect of ${NO_3}^-$ and ${NH_4}^+$ on the adventitious root growth and eleuthroside synthesis of Eleutherococcus koreanum in 5 L-bioreactor culture. The change in the medium components was also measured during culture. The fresh weignt of adventitious root reached to the highest level of 30.8 g FW/L in the presence of both 50 mM ${NO_3}^-$ and 10 mM $NH_4^+$, representing 3.6-fold increase compared to the 60 mM ${NH_4}^+$ alone. However, as the increase of the portion of ${NH_4}^+$, the root growth was decreased. However, the maximum eleutheroside B, E and E1 contents were $57.3{\mu}g/g$ DW, $188.4{\mu}g/g$ DW and $47.3{\mu}g/g$ DW, with 30 mM, 60 mM and 15 mM total nitrogen source, respectively. Fresh weight of adventitious root increased up to 6.8-fold of inoculum size within 9 weeks. The amounts of ${NH_4}^+$, $K^+$, ${NO_3}^-$ and ${PO_4}^-$ were decreased during culture periods. Based on these results, we suggest that various further studies are required to increase the biomass and the useful secondary metabilites.

생물반응기를 이용하여 섬오갈피 부정근 증식시 부정근의 생장과 유용 이차대사산물인 eleutheroside 류 생산에 미치는 ${NO_3}^-$와 ${NH_4}^+$의 영향 및 배양기간에 따른 배지성분의 변화량을 분석하였다. 부정근 증식은 질소농도가 50 mM ${NO_3}^-$와 10 mM ${NH_4}^+$ 농도비율로 첨가된 처리구에서 최대(30.8 g FW/L)에 이르러 60 mM ${NH_4}^+$ 첨가에 비해서는 3.6배 증식되었다. 또한 ${NH_4}^+$의 첨가농도비율이 높아질수록 부정근의 증식은 감소하였다. 그러나 eleutheroside B는 총 질소량이 30 mM 처리에서 $57.3{\mu}g/g$ DW, E는 60 mM 처리에서 $188.4{\mu}g/g$ DW의 함량으로 생산량이 가장 많았다. 반면에 eleutheroside $E_1$은 총 질소량이 15 mM 처리에서 $47.3{\mu}g/g$ DW로 생산량이 가장 많았다. 부정근 최대생장은 초기 접종량 대비 배양 9주만에 6.8배가 증식되었고, 배양기간별 배지 성분의 변화에서 ${NH_4}^+$, $K^+$, ${NO_3}^-$와 ${PO_4}^-$는 배양기간이 경과할수록 함유량이 감소하였다. 이러한 결과로 보아 부정근의 생장과 유용이차대사산물의 함량을 높이기 위하여 다양한 처리의 연구가 수행되어야 할 것으로 생각된다.

Keywords

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