• Korean Journal of Plant Resources
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• v.31 no.6
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• pp.719-724
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• 2018

Methicillin-resistant Staphylococcus aureus (MRSA) is a bacterium responsible for a number of infections in humans that are difficult to treat, and as a result, is a substantial contributor to morbidity and mortality. In the present study, in search of natural products capable of inhibiting this multidrug-resistant bacterium, we investigated the antimicrobial activity of Lysimachia clethroides Duby root. The antibacterial activities of EtOH extract of Lysimachia clethroides Duby root and its n-hexane, EtOAc, n-BuOH and water fractions were evaluated against 15 strains of methicillin-resistant staphylococcus aureus (MRSA) and 1 standard methicillin-susceptible S. aureus (MSSA) strain by using the minimal inhibitory concentrations (MICs) assay, colorimetric assay using MTT test, checkerboard dilution test. Antimicrobial activity of n-hexane fraction of Lysimachia clethroides Duby root was remarkable. Against the 16 strains, the minimum inhibitory concentrations (MICs) were in the range of $31.25-62.5{\mu}g/ml$ and FICI values for n-hexane fraction of Lysimachia clethroides Duby root+AM and n-hexane fraction of Lysimachia clethroides Duby root+OX were checkerboard method performed using the MRSA, MSSA and one clinical isolate strains via MICI 0.12-1 and 0.25-0.75, showing the increase of synergistic effect. When combined together, these antibiotic effects were dramatically increased. These effective combinations could be new promising agents in the management of MRSA.

• YAKHAK HOEJI
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• v.37 no.4
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• pp.325-330
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• 1993

Four flavonoide glycosides and (-)-Epicatechin were isolated from the aqueous extracts of dried whole part of Lysimachia clethroides Duby(Primulaceae). They were 3-0-Methyl-quercetin-7-0-[$\alpha$-L- rhamnopyranosyl (1-2) glucopyranoside], Quercetin-3-0-$\beta$-D-glucopyranoside, Kaempferol-3-0-$\beta$-D-glucopyranoside, Kaempferol-3-0-[$\alpha$-L-rhamnopyranosyl (1-6)-$\beta$-D-glucopyranoside] and (-)-Epicatechin. 3-0-[$\alpha$-L-rhamnopyranosyl (1-6)-$\beta$-D-glucopyranoside]and (-)-Epicatechin. 3-0-Methyl-quercetin-7-0-[$\alpha$-L-rhamnopyranosyl (1-2)-$\beta$-D-glucopyranoside] and (-)-Epicatechin were first isolated from this plant. Their structures were elucidated by spectral analysis [$^{1}$H-, $^{13}C-$ NMR, $^{1}$H-$^{1}$H-COSY, DEPT-analysis, HMQC(Heteronuclear multiple quantum coherence), FAB-MS].

• Korean Journal of Food Science and Technology
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• v.33 no.6
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• pp.774-783
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• 2001

The ethanol extract of 77 species of edible and medicinal plants were examined antimicrobial activity against 5 strains of Listeria monocytogenes ATCC 19111, ATCC 19112, ATCC 19113, ATCC 19114 and ATCC 15313 by optical density using Bioscreen C. The ethanol extract of Siegesbeckia glabrescens Makino, Jeffersonia dubia Benth, Aquilaria agallocha Roxburgh, Lysimachia clethroides Duby and Nardostachys chinensis Batal. exhibited comparatively strong growth inhibition effect on 5 strains of L. monocytogenes at 1000 ppm level in broth. The minimum inhibitory concentration (MIC) of ethanol extract of Lysimachia clethroides Duby was $100{\sim}500\;ppm$ on 5 strains of L. monocytogenes. The MIC of the n-hexane and chloroform fraction of the extract were same concentration as $50{\sim}100\;ppm$. The n-hexane fraction of Lysimachia clethroides Duby showed strong growth inhibition at 25 ppm on Vibrio parahaemolyticus for 72 hr at $30^{\circ}C$ and at 50 ppm on Bacillus cereus and at 500 ppm on Staphylococcus aureus and Escherichia coli.

• The Korean Journal of Mycology
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• v.36 no.2
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• pp.196-198
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• 2008

A rust disease occurred on Lysimachia clethroides in Deogyusan National Park, Jeollabukdo. The typical symptoms of the disease appeared as small, yellowish spots on leaves at first. The spots then turned yellowish brown and powdery mass of blackish brown spores appeared on the lesions. Severely infected leaves blighted and eventually shriveled. Teliospores were yellowish brown in color, clavate or fusiform in shape and $38{\sim}62\;{\times}\;14{\sim}22\;{\mu}m$ in size, the apex rounded or obtuse, the base attenuated. Pedicels hyaline, persistence, up to $75{\mu}m$ long. The causal fungus was identified as Puccinia dieteliana based on mycological characteristics and host specificity.

• Applied Biological Chemistry
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• v.47 no.2
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• pp.265-269
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• 2004

This study was conducted to develop physiologically active plant materials from medicinal plants. Crude extracts and solvent fractions prepared from Lysimachia cletroides Duby were tested for their antioxidant and antihypertensive activities. For ellucidating antioxidant potential, inhibition rate on linoleic acid peroxidation, as well as scavenging activities on superoxide anion and 1,1-dipicrylphenylhydrazyl (DPPH) radical were evaluated. For analyzing antihypertensive effect, inhibitory activity on angiotensin converting enzyme (ACE) was done. Methanol extract of L. cletroides showed potent inhibition activity of 83% on linoleic acid peroxidation, which was more effective than -2% of ${\alpha}-tocopherol$ at $25\;{\mu}g/ml$. Methanol and water extracts exhibited strong scavenging activities of $86{\sim}109%$ and $96{\sim}122%$ on superoxide anion radical which was higher than $-4{\sim}69%$ of ascorbic acid at $5{\sim}200\;{\mu}g/ml$. Hexane, ether and ethylacetate fractions possessed 133, 100 and 88% inhibitory activities on ACE at $4,000\;{\mu}g/ml$, respectively. From the results, it was expected that Lysimachia cletroides could be a new antioxidant and antihypertensive resource.

• Journal of Forest and Environmental Science
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• v.16 no.1
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• pp.1-16
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• 2000

This study was carried out to examine the process of plant succession through vegetation invasion and to select appropriate endemic plant species for rapid stabilization and good visual effect on cut-slopes of forest roads. Establishing total of sixty $1m{\times}1m$ sample plots. fifteen for each forest road constructed in the year of '93 (six-year-old), '95(four-year-old), '97(two-year-old), and '98(one-year-old), the ecological attributes of invading vegetation on cut-slopes were analyzed. The results are summarized as follows: 1. The rate of vegetation coverage was highly associated with soil hardness and aspect of cut-slope. Higher rate of vegetation coverage was caused by larger number of invading plant species. 2. The dominant woody species were Rubus crataegifolius, Rhus chinensis, Lespedeza bicolor, Salix hulteni, Alnus hirsuta, and Pinus densiflora. The visual attractive for the fruit of Rubus crataegifolius and the autumn coloration of Rhus chinensis was noteworthy. The dominant herbaceous species were Youngia sonchifolia, Spodiopogon sibiricus, and Lysimachia clethroides in all study forest roads. Spring flower of Potentilla freyniana and Viola rossii: summer flower of Lysimachia clethroides, Commelina communis, Glycine soja. Persicaria sieboldi, and Oenothera odorata: and autumn flower of Artemisia stolonifera and Impatiens textori were abundant and remarkable. 3. The diversity index of woody species tended to be increased as years passed after construction, and that of herbaceous species were decreased. 4. The dominance of Th of dormancy form was early high in the first year of construction, getting decreased thereafter. And that of MM + M + N was increased as years passed after construction. but that of Ch+H+G+Th+HH was decreased. 5. The degrees of succession were estimated by 359, 111, 97, and 87 for the construction year of '93, '95, '97, and '98. respectively, increased as years passed after construction.

• Korean Journal of Environmental Biology
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• v.24 no.4
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• pp.408-415
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• 2006

Serpentines soil have high values of magnesium and low values of calcium, and are usually deficient in N and P, but rich in iron, Ni, silicates. We investigated serpentine soil properties and measured the content of nutrient elements and heavy metals in shoots and root of plant species which were in common at serpentine and non-serpentine areas in Andong, Korea. The soils showed higher pH value above 6.9. The contents of Ni, Cr, Fe and Mg of serpentine soils exhibited 77, 27, 5.5 and 12.5 times more than in non-serpentine soils, respectively. The content of Na was almost same but K was two times higher in non-serpentine soil, compared with serpentine soil. The contents of nutrient element such as K, Ca, Na and P in serpentine plants did not show conspicuous differences with non-serpentine plants. On the other hand, the concentrations of Ni, Cr, Fe, Mg and Mg/Ca were very high in plant on serpentine area. The all plant species collected at the serpentine site were bodenvag plants, which are not restricted to a specific type of substrate. By the plant species and parts of plant tissues, the absorption levels and patterns showed high variation and were species-specific. Carex lanceolata, Lysimachia clethroides and Cynanchum paniculatum contained much chromium and Eupatorium chinense and C. paniculatum exhibited high contents of Ni. In leaf tissue, C. lanceolata, Rubus parvifolius, Festuca ovina, Quercus serrata, and L. clethroides took comparatively large amount of Cr in serpentine area. E. chinense contained large amount of Ni, Cr and Fe in a leaf tissue. The stem of Galium verum, Juniperus rigida included high amount of Cr, Ni and Fe. And C. paniculatum absorbed large amount of Ni and Cr in the stem.

• Journal of Life Science
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• v.14 no.3
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• pp.509-513
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• 2004

Inhibitory activities of 58 methanol extracts, which were prepared from different parts of 55 kinds of edible and medicinal plants, against human thrombin were evaluated. Analysis of the anti-coagulation activity determined by thrombin time enabled us to select 8 herb extracts as possible sources of anti-thrombosis agent. Further analysis of anti-thrombosis activity determined by activated partial thromboplastin time and fibrinolytic activity, and heat stability and inhibition against other proteolytic digestive enzymes provided new information that Pimpinella brachycarpa, Lysimachia clethroides, and Salix gracilistyla could be used as a potential anti-thrombosis agent. Our results suggest that edible and medicinal plants could be the potential source of thrombin inhibitor.

• The Korean Journal of Ecology
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• v.20 no.1
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• pp.27-33
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• 1997

Species composition and the amount of nutrients absorbed by regenerating plants on a pine forest in the immediate postfire year were compared with those in an unburned pine forest in Kosung, Kangwon Province. Pteridium aquilinum var. latiusculum, Cyperus amuricus, Lespedeza biolor, Quercus serrata, Lysimachia clethroides were the most abundant species in burned area. In unburned area. Quercus mongolica, Rhododendron mucronulatum, Carex humilis, Rhododendron schlippenbachii, Spodiopogon sibiricus were the most abundant species. Standing biomass of understory vegetation in burned and unburned area was 170.2 g $D.W/m^2$ and 171.3g $D.W/m^2$, respectively. Nutrient concentrations of plants in burned area, especially for phosphorus and potassium, were higher than those in unburned area. The amounts of nutrients absorbed by understory plants in burned and unburned area were 37.4 and 33.6 kg/ha for N, 0.36 and 0.19 kg/ha for P, 30.6 and 18.8 kg/ha for K, 8.5 and 7.8 kg/ha Ca, 5.2 and 5.7 kg/ha for Mg, respectively. This suggests that regenerating vegetation can hold the significant amount of nutrients, although there may be considerable losses of nutrients from ecosystem after fire.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.5 no.1
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• pp.19-27
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• 2002

To investigate the dominant species and factors related with plant coverage by road structures and forest environment factors, forest roads elapsed from one year to twelve year after construction had been selected in six county(Gochang-gun, Muju-gun, Imsil-gun, Jangsu-gun, Jeongup-shi and Jinan-gun), and 20m segments were continuously set up in each road. The results obtained from this study are summarized as follows: The species diversity of Gochang, Muju, Imsil, Jangsu, Jeongup and Jinan were 1.304, 1.267, 1.308, 1.193, 1.289 and 1.018, respectively. In process of years, plant coverage was increased gradually and average of plant coverage was 15.3% in forest roads which elapsed three year, and was 86.5% in forest roads which elapsed nine year after construction. The dominant species in the cutting slope of surveyed area were covered with Arundinella hirta, Pinus rigida, Miscanthus sinensis var. purpurascens, Artemisia princeps var. orientalis, Pinus densiflora, Oplismenus undulatifolius, Rubus coreanus, Lysimachia clethroides, Lespedeza bicolor, and Alnus hirsuta of the 152 species. The high correlated factors between plant coverage and variables in cutting slopes appeared elapsed year, soil hardness, mean annual precipitation, vertical grade, inslope and arid humidity in surveyed area.