Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibitory Activities of Edible and Medicinal Herbs Against Human Thrombin

식용 및 약용 산채류로부터 트롬빈 저해물질의 탐색

  • 권정숙 (안동대학교 식품영양학과) ;
  • 권윤숙 (안동대학교 식품영양학과) ;
  • 김영숙 (안동대학교 식품영양학과) ;
  • 권기석 (안동대학교 생명자원과학부) ;
  • 진익렬 (경북대학교 미생물학과) ;
  • 류기철 (㈜대평 천연물신소재 개발연구소) ;
  • 손호용 (안동대학교 식품영양학과)
  • Published : 2004.06.01
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Abstract

Inhibitory activities of 58 methanol extracts, which were prepared from different parts of 55 kinds of edible and medicinal plants, against human thrombin were evaluated. Analysis of the anti-coagulation activity determined by thrombin time enabled us to select 8 herb extracts as possible sources of anti-thrombosis agent. Further analysis of anti-thrombosis activity determined by activated partial thromboplastin time and fibrinolytic activity, and heat stability and inhibition against other proteolytic digestive enzymes provided new information that Pimpinella brachycarpa, Lysimachia clethroides, and Salix gracilistyla could be used as a potential anti-thrombosis agent. Our results suggest that edible and medicinal plants could be the potential source of thrombin inhibitor.

혈전 관련 질환 예방 및 혈류개선 기능성 식품의 개발을 목표로, 식용 및 약용 산채류 55종의 메탄을 추출물을 조제한 후 트롬빈 저해활성을 검색하였다. 그 결과, 대조군으로 사용된 아스피린보다 우수한 항혈전 활성을 나타내는 붉나무, 사삼, 우산나물, 쥐다래, 참나물, 할미밀망, 큰까치수영, 첫버들 추출물 등 8종을 선별하였으며, aPTT, 혈전 용해활성, 열 안정성, 기타 소화효소 저해활성 등을 평가하며 참나물, 큰까치수영 및 갯버들 추출물을 최종 선별하였다. 참나물의 경우 열안정성이 낮은 반면 소화효소 비저해로 인해 그 이용성이 높으며, 큰 까치수영 및 갯버들의 경우 부분적인 소화효소 저해능이 있으나 우수한 항혈전활성 및 열 안정성을 나타내어 다양한 식품소재로 개발이 가능함을 확인하였다.

Keywords

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