• Korean journal of applied entomology
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• v.4
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• pp.65-66
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• 1965

This study was intended to know the histological damage of the gall on the leaves, peduncles and calyx of Lycium chinense $M_{ILL}$ formed Eriophyes kuko $K_{ISHIDA}$ during 1962 to 1965. The results are summarized as follows; 1. Eriophyes kuko $K_{ISHIDA}$ fed on only the Palisade layers of the loaves of Lycium chinense $M_{ILL}$ were injured by boring. 2. From the 1st to 10th day, some parts of the spongy layer of the Lycium chinense $M_{ILL}$ leaves were injured, but variation were not significant in this period. 3. From the 15th day, Eriophyes kuko $K_{ISHIDA}$ injured not only the spongy layer of Lycium chinense $M_{ILL}$. leaves but also the great part of e chloroplast of the loaves, and the sponginess caves were appeared inside of the galls.

• Archives of Pharmacal Research
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• v.8 no.4
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• pp.249-252
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• 1985

$N_{9}$-Formylharman, 1-carbomethoxy-$\beta$-carboline and perlolyrine with an unidentified alkaloid ($C_{10}H_{13}NO_{2}$, $M^{+}$ 179.094) have been isolated from the fruits of Lycium chinense Miller (Solanaceae) and characterized on the basis of chemical and physical evidence.

• Journal of the Korean Applied Science and Technology
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• v.39 no.1
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• pp.27-33
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• 2022

The purpose of this study was to investigate the functionality of Lycium chinense Miller. as a functional cosmetic material. The total polyphenol content in the extract was 17.3 mg/mL, and as a result of confirming cytotoxicity through MTT assay before the enzyme experiment, it was confirmed that it did not affect cell growth at all concentrations below 1000 ㎍/mL. As a result of conducting DPPH radical scavenging tests as an antioxidant efficacy test, it showed 84.97% scavenging ability at a concentration of 1000 ㎍/mL, and showed very excellent antioxidant efficacy in SOD-like activity test with a result of 80.54% at 1000 ㎍/mL. The measured elastase inhibitory activity, the inhibition rate of 50.93% was observed at 1000 ㎍/mL, indicating that the extract of Lycium chinense Miller. was effective in inhibiting elastase. All these findings suggested that 70% ethanol extract of Lycium chinense Miller. was confirmed as a functional cosmetic material by confirming its antioxidant and anti-wrinkle efficacy.

• Natural Product Sciences
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• v.17 no.2
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• pp.104-107
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• 2011

In order to facilitate the quality control of betaine from the fruits of Lycium chinense, we have developed a rapid and simple method for quantitative determination. Determination was achieved on a Discovery C18 column with an isocratic solvent system of 0.32% perfluoropentanoic acid aqueous-acetonitrile at a flow-rate of 0.5 mL/min and detected an ELSD. The method was reproducible with intra- and inter-day variations of less than 6% (R.S.D). The recoveries were in the range of 90.01~100.05%. The method turned out to be fast and simple, furthermore, to have a good selectivity and sensitivity for the quantity determination of betaine in the fruits of L.chinense.

• Korean Journal of Medicinal Crop Science
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• v.19 no.2
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• pp.126-135
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• 2011

Various herbal medicinal extracts were examined for the development of cosmetic products with anti-oxidative and anti-wrinkle activity. First, total polyphenol contents and DPPH radical scavenging activities of herbal medicinal extracts were measured. Most herbal samples, except for DW extracts of Portulaca oleracea, Caesalpinia sappan, Taraxacum platycarpum, Carthamus tinctorius, and 70% EtOH extracts of Taraxacum platycarpum and Carthamus tinctorius, showed DPPH radical scavenging activity over 80% at a concentration of $400{\mu}g/m{\ell}$. SOD-like antioxidant activity of DW extracts of Syzygium aromaticum, DW extracts of Eriobtrya japonica and 70% EtOH extracts of Sophora japonica was measured as 40%, 35% and 80%, respectively at a dry matter concentration of $50{\mu}g/m{\ell}$. In elastase inhibition assay, DW extracts of Lycium chinense ($50{\mu}g/m{\ell}$) and 70% EtOH extracts of Areca catechu ($50{\mu}g/m{\ell}$) showed 50% and 40% of inhibition, respectively. At a concentration of $1.250{\mu}g/m{\ell}$, DW extracts of Lycium chinense and 70% EtOH extracts of Areca catechu showed 10% and 30% of collagenase inhibition, respectively. Skin and lotion samples were prepared using the two herbal extracts of high anti-wrinkle activity: Lycium chinense extract and Areca catechu extract. The storage stability of skin and lotion containing each of the selected herbal extracts was evaluated. pH and viscosity were used as stability indicators for the stability test under different storage temperatures and freeze-thaw cycle conditions. The skin and lotion containing each of DW extract of Lycium chinense and 70% EtOH extract of Areca catechu was showed high pH and viscosity stability. The skin and lotion containing DW extracts of Lycium chinense showed relatively higher stability than the skin and lotion containing 70% EtOH extract of Areca catechu, at cycle chamber and freeze-thaw conditions. In summary, these results indicated that cosmetics containing DW extract of Lycium chinense were relatively stable, and this herbal extract could be used as a stable functional cosmetic material.

• KSBB Journal
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• v.13 no.5
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• pp.569-576
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• 1998

The effects of carbohydrates, hormones and elicitors on both cell growth and betaine production were investigated in the cell cultures of Lycium chinense Mill. The maximum effect of glucose and sucrose was observed in cells cultured in the presence of 3% and 7% for cell growth and betaine production, respectively. The effect of hormones on cell growth and betaine production was prominent in the presence of 10 ${\mu}$M 2, 4-D, 10 ${\mu}$M NAA and 2.5 ${\mu}$M IAA, whereas cell growth and betaine production were excellent at 2.5 ${\mu}$M BA and 10 ${\mu}$M BA, respectively. Abiotic elicitors such as KCI, MnCl2 and NaCl exhibited an inhibitory role on cell growth in all treatment groups. Betaine production was increased according to increase of concentration of abiotic elicitors. methanol-soluble and insoluble components as biotic elicitor remarkably inhibited cell growth from 2 mg and 6 mg, respectively. Betaine production was increased maximally at 2 mg of biotic elicitors. When growth medium was switched to production medium at two stage culture, it resulted that cell fresh weight and dry weight decreased but betaine content increased about 2.2-fold.

• Journal of Biosystems Engineering
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• v.25 no.2
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• pp.151-158
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• 2000

Detachment rate of mature fruits of the Lycium chinense Mill with various frequency, amplitude, and shaking time using an experimental vibratory system was analyzed. The experimental vibratory system was designed such that the frequency, amplitude, and shaking time could be controlled easily. The detachment rate increased as the frequency increased at constant amplitude and increased as the amplitude increased at constant frequency. The contents of the detached immature fruit, however, also increased as the frequency and amplitude increased, which was undesirable for the detachment efficiency considering the only mature 900 rpm and amplitude of 35 m for the shaking time of three seconds.

• Journal of Korean Society of Forest Science
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• v.95 no.5
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• pp.585-590
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• 2006

In vitro-grown axillary shot-tip meristems of Lycium chinense Mill. from cold-acclimated plant were successfully cryopreserved using a vitrification technique. After loading for 15 minutes with a mixture of 2.0 M glycerol and 0.4 M sucrose ($20^{\circ}C$), small segments (1-2 mm, 3-4 mm, and 5-6 mm) were cut from axilary buds and exposed to the cryoprotectant solution containing 30% glycerol, 15% ethylen glycol,15% dimethyl sulfoxide (DMSO), and 0.4 M sucrose at $0^{\circ}C$ for 30-120 minutes prior to direct plunge into liquid nitrogen (LN). After rapid thawing ($40^{\circ}C$), the segments were washed with MS medium containing 1.2 M sucrose for 0-35 minutes, and then transferred onto recovery-growth medium. The highest survival rate (about 90%) was obtained with cold-hardening treatment, and cryopreserved explants were successfully recovered to plantlets. No abnormal morphological changes were observed with the recovered plants after cryopreservation.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.48 no.2
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• pp.113-121
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• 2022

In this study, fermented Lycium chinense (L. chinense) extract was used to analyze the efficacy of nitric oxide (NO) and anti-aging inhibition to verify its usefulness as a functional cosmetic material. L. chinense was extracted with hydrothermal and 70% ethanol as a solvent, fermented, concentrated, and freeze-dried to prepare a sample, followed by MTT assay, NO inhibition assay, western blot assay, and UPLC analysis.As a result of the analysis of NO inhibitory efficacy, fermented L. chinense water extract (FLW) and fermented L. chinense 70% ethanol extract (FLE) showed excellent NO inhibitory efficacy of 47.96% and 56.71%, respectively. As a result of measuring the expression patterns of the wrinkle-related proteins MMP-1, and TRPV-1 through Western blot, both FLW and FLE confirmed the inhibitory efficacy of MMP-1 and TRPV-1. Based on the results of the experiment, the fermented L. chinense extract is expected to have a high application value as a functional cosmetic material related anti-inflammatory, and anti-aging.

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.23-27
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• 1994

The growing condition and the point at issue were investigated for utilization as a fundamental data of the Boxthorn research in the growing farm household, which was chief producing district of Box-thorn in Chongyang, Chungmam province. The results were summarized as follow'; Lycium fruit wasproduced 445M/. in the 170.4ha. In practical cultivation, major numbers of decapitation were threetimes,, and planting distance was dense planting, and also amount of applied fertilizer was beyondbasic-fertilizer level and number of split application was abundant.Numbers of harvesting were six-ten times, and many labor was requiremented.The issues in cultivation were development of processing technique, supplement of varieties whichhave disease tolerance, superior quality and high yielding, improving of cultivation methods for laborsaving and magnification of demand.