Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Cryopreservation of in Vitro Grown Axillary Shoot-tip Meristems of Lycium chinense by Vitrification

  • Yang, Byeong-Hun (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Song, Min-Jung (Institute of Life Science and Biotechnology, Korea University) ;
  • Ahn, In-Suk (Department of Biological Science, Myongii University) ;
  • Kim, Hyun-Tae (Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Young-Goo (Department of Forestry, Kyungpook National University)
  • Received : 2006.06.27
  • Accepted : 2006.09.01
  • Published : 2006.10.30
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Abstract

In vitro-grown axillary shot-tip meristems of Lycium chinense Mill. from cold-acclimated plant were successfully cryopreserved using a vitrification technique. After loading for 15 minutes with a mixture of 2.0 M glycerol and 0.4 M sucrose ($20^{\circ}C$), small segments (1-2 mm, 3-4 mm, and 5-6 mm) were cut from axilary buds and exposed to the cryoprotectant solution containing 30% glycerol, 15% ethylen glycol,15% dimethyl sulfoxide (DMSO), and 0.4 M sucrose at $0^{\circ}C$ for 30-120 minutes prior to direct plunge into liquid nitrogen (LN). After rapid thawing ($40^{\circ}C$), the segments were washed with MS medium containing 1.2 M sucrose for 0-35 minutes, and then transferred onto recovery-growth medium. The highest survival rate (about 90%) was obtained with cold-hardening treatment, and cryopreserved explants were successfully recovered to plantlets. No abnormal morphological changes were observed with the recovered plants after cryopreservation.

Keywords

Acknowledgement

Supported by : Kyungpook National University

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