• Korean Journal of Food Science and Technology
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• v.38 no.2
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• pp.222-231
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• 2006

Deoxynivalenol (DON)-contaminated barley was treated with 0.1 M $Na_2CO_3$ solution to reduce DON content. DON content of barley was reduced from 2.08 to 0.67 ppm. Bread was made with 10, 20, and 30% DON-reduced barley flour added to white wheat flour. Farinogram showed water absorption and arrival time increased, while stability and mechanical tolerance index decreased when DON-reduced barley flour was added to white wheat flour. Gelatinization temperature, temperature at maximum viscosity, and maximum viscosity increased in amylogram with increasing addition of DON-reduced barley flour. Loaf volume of bread decreased with increasing amount of DON-reduced barley flour, while loaf weight increased. Barley flour pH increased by treatment with$Na_2CO_3$, and pH reduction decreased fermentation rate of yeast. Volume and size of gluten matrix decreased and crumb hardened in bread made with DON-reduced barley flour. Acceptabilities for color and texture were low in bread made with DON-reduced barley flour. Addition of DON-reduced barley flour at 30% reduced overall acceptability, whereas no significant difference in overall acceptability was observed when DON-reduced barley flour was added at 10 and 20%.

• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.390-395
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• 2006

Effects of exogenously foliar applied glycine betaine (GB) on the growth and contents of osmolyte in tomato seedling was investigated. Plants treated with exogenous glycine betaine induced better biomass production and plant height during chilling stress than the untreated plants. The total soluble sugar contents in GB foliar-applied plants lower than that of untreated plants 28 days after foliar application. Total water soluble protein contents in GB foliar-applied plants did not change 28 days after chilling stress. In untreated plant, it decreased rapidly in the beginning of chilling stress. Proline contents in untreated plants rapidly increased by the beginning of chilling stress, and then slightly decreased during the next 3 weeks. However proline contents in GB foliar-applied plants did not change during the 28 days chilling stress period. The results suggest that foliar application of GB is a effect methods to increase the chilling tolerance of tomato seedlings in protected cultivation system at low temperature season.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.25 no.4
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• pp.251-264
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• 1992

Distribution of indicator species of copepods and chaetognaths were studied as an indicator species of water mass in the southeastern area of the Yellow Sea. Undinula darwini, Lucicutia flavicornis, Pleuromamma gracilis, Euchaeta resselli, Euchaeta plane and Sagitta enflata were found to be reliable indicator species for determining warm water mass. Of these species, E. plana and E. rusrelli have a weak tolerance on the low temperature. Sagitta crassa was indicator species of neritic waters; Sagitta bedoti was that of mixing waters. Centropages abdominalis represented neritic cold waters. In February, U darwini, L. flavicornis, P. gracilis, E. russelli, E. plana and S. enflata occurred in the western waters of Cheju-Do where warm waters over $14^{\circ}C$ occupied. Centropages abdominalis occurred in the northern area beyond Chindo with water temperature less than $10^{\circ}C$. E. plana, E. russelli and S. bedoti were found at the regions between Cheju-Do and Chindo where the water temperature was $12- 14^{\circ}C$ corresponding to the mixing waters. Based on cluster analysis and T-S diagram in February three different water masses were identified from the south to the north. In August, water masses were analyzed at two different layers, 0-20m and 20m- bottom layers, separated by bhermocline depth. In 0-20m layer, E. plana and E. russelli were found from the western waters of Cheju-Do to Daehuksando. In 20m- bottom layer, E. russelli and E plena occurred at the northwestern waters of Cheju-Do with the water temperature warmer than $12^{\circ}C.\;C.$ abdominalis was found at the northern area beyond Chindo. Based on the cluster analysis and T-S diagram in August three different water masses at 0-20m and 20m-bottom layers were identified from the coast to the offshore. C. abdominalis was found at the adjacent water of Chindo at 0-20m layer and the northern area beyond Chindo at 20m~bottom layer. This fact suggested that the cold water mass existed at tile adjacent waters of Chindo in summer.

• Korean Journal of Agricultural Science
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• v.10 no.1
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• pp.28-60
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• 1983

This study was carried out to define the effects of external factors including temperature, moisture, oxygen and light quality on the germination of sesame seeds and to investigate the change of major chemical constituents of seeds during germination. The results obtained are summarized as follows: 1. The average germination ratio was from 95.8% to 97.2% when it was tested every $5^{\circ}C$ intervals from $20^{\circ}C$ to $35^{\circ}C$ and no significant difference in germination ratio was found within $20^{\circ}C$ to $35^{\circ}C$. But the germination ratio dropped rapidly to 32.2% when seeds were germinated at $15^{\circ}C$ and the coefficient of variation become greater(77%) 2. The days required for germination ranged from 1.16 to 1. 64 at the temperatures of $35^{\circ}C$ to $25^{\circ}C$ and they were 3.07 and 10.4 at the temperatures of $20^{\circ}C$ and $15^{\circ}C$, respectively. 3. Considering the germination ratio and days needed, $15^{\circ}C$ was assumed to be the minimum temperature for germination practically and this temperature is recommended for testing low temperature tolerance of seed germination of sesame cultivars. 4. The varieties shown the highest low temperature tolerance were Shirogoma and Turkey. The next varieties shown some degree of low temperature germination were Suweon #29, Naebok and IS 58. The varieties with 70 to 80% of germination ratio were Maepo, Suweon #14, Kimpo, Moondeok, and Haenam. Among the 90 varieties tested, the varieties with comparatively high degree of low temperature tolerance were about 10%, and 70% of the low temperature tolerant varieties were domestic varieties. 5. At $12^{\circ}C$ the Shirogoma was the only variety which showed over 50% of germination ratio, 71.4% of the varieties showed less than 20% of germination ratio. When the temperature was raised to $27^{\circ}C$ 18 days after placement at $12^{\circ}C$ all the varieties showed over 90% of germination ratio within 2days. 6. The amounts of water imbibition needed for seed germination were 0.48 to 0.62 times of the seed dry weight at $25^{\circ}C$ and were significantly different among sesame cultivars. About 63% of water required for germination was imbibed in 2 hours after placement of seeds under the germination condition. 7. Under saturated moisture condition the average germination ratio was 0.42%. In the soil of which water potential was -0.4bar 64.8% of the seeds germinated and the most adequate soil water potential for sesame seed germination was about -0.4 to -5.5 bar. The germination ratio decreased as the soil water potential declined below -5.5 bar. 8. Six out of 10 varieties were not influenced by 5% of oxygen in air germination chamber, while varieties such as Yecheon, PI 158073, IS 103 and Euisangcheon showed 64 to 91% of germination under the 5% oxygen content. Under anaerobic condition, cotyledones were not emerged but only hypocotyl was emerged and elongated. The germination ratio of IS 103 decreased significantly under anaerobic condition. 9. When the seeds were dried for 24 hours after 12 hours imbibition of water, the seeds of Cheongsong did not lose their germination ability and 27.5% was germinated but Suweon #9 and Early Russian failed to germinate. However, the germination ratio of IS 103 decreased when the seed were dried 24 hours after 4 hours imbibition of water and the germination ability of IS 103 was maintained even though the seeds were dried for 24 hours after 24 hours imbibition of water. 10. During germination, sugar content of sesame seed increased rapidly and activity of ${\alpha}$-amylase increased gradually while starch content decreased significantly. The rates of increase in sugar content and enzyme activity and decrease in starch content were significantly lower at $15^{\circ}C$ compared with those at $25^{\circ}C$. 11. During germination of sesame seeds, lipid content in the seeds dropped rapidly and the activity of alkaline lipase increased significantly at early stage of germination. The rate of decrease in lipid content and increase in emzyme activity was lower at $15^{\circ}C$ than at $25^{\circ}C$. 12. Four out of 6 varieties were not affected in germination by light wave length. But Suweon #8 was inhibited in germination by 600-650nm. and IS 103 by 600 to 650nm and 500 to 550nm of light wave length. Suweon #8 showed high germination ratio under 650 to 760 nm and 500 to 560nm, and IS 103 under 400 to 470nm and complete darkness. 13. The germination ratios increased significantly in the seeds of which 1000 grain weight is heavier. When the seeds were placed at soil 4cm deep, Cheongsong and Early Russian failed to emerge their cotyledones, but Suweon #9 and IS 103 showed 32.5 and 50% cotyledone emergence, respectively. The extracts from sesame plant and soil where the sesame was cultivated previously did not affect in the-germination of sesame seeds. 14. The covering by black or transparent polyethylene films increased germination ratio compared with uncovered seeds. The covering was effective in shortening the days needed for germination and in improving the early seedling growth, number of capsules per plant and grain yield. Difference was not so seizable between the two polyethylene films but the transparent film appeared somewhat more effective than the black one. 15. Simcheon, Cheongsong. Suweon #9. PI 158073 and IS 103 showed lower rate of water absorbtion by seed during germination and Suweon #8, Suweon #26, Orotall and Euisangcheon showed high increase in seed weight after water absorbtion by seed.

• Asian Journal of Turfgrass Science
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• v.25 no.2
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• pp.138-146
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• 2011

This study was carried out to select salt tolerant zoysiagrass breeding lines. Eighty two native zoysiagrasses collected from S. Korea were used in this study. Saline water were prepared by mixing sea water and tap water. ECw levels of saline water treated ranged from 2 to $3dS{\cdot}m^{-1}$. Zoysiagrass planted in pot by sprigging were soaked into the plastic box containing saline water. Winter injury was investigated under the pot condition. Most of Z. japonica types did not show winter injury. But Z. tenuifolia type, Z. matrella type, and Z. sinica type showed winter injury under the pot condition at Cheonan area. NaCl level in soil was increased from 0% to 0.51% by treatment of saline water. Soil ECe measurement showed upto $170dS{\cdot}m^{-1}$. Z. tenuifolia type (Z5034), Z. matrella type ('Konhee', Z4109, 'Semill'), Z. japonica type (Z1055, Z1040, Z1008, 'Zenith', 'Millock') and medium leaf type zoysiagrass (Z6096, Z6118, Z6021, Z6074) resulted in below 30% leaf firing under the saline condition. This approach might be useful for selecting salt tolerant breeding lines.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.240-249
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• 2022

Measuring chlorophyll fluorescence (CF) is a useful tool for assessing a plant's ability to tolerate abiotic stresses such as drought, waterlogging and high temperature. Korean ginseng is highly sensitive to water stress in paddy fields. To evaluate the possibility of non-destructively diagnosing waterlogging stress using chlorophyll fluorescence (CF) imaging techniques, we screened 57 ginseng accessions for waterlogging tolerance. To evaluate waterlogging tolerance among the 2-year-old Korean ginseng accessions, we treated ginseng plants with water stress for 25 days. The physiological disorder rate was characterized through visual assessment (an assigned score of 0-5). The physiological disorder rates of Geumjin, Geumsun and GS00-58 were lower than that of other accessions. In contrast, lines GS97-62, GS97-69 and GS98-1-5 were deemed susceptible. Root traits, chlorophyll content and the reduction rates decreased in most ginseng accessions. Further, these metrics were significantly lower in susceptible genotypes compared to resistant ones. All CF parameters showed a positive or negative response to waterlogging stress, and this response continuously increased over the treatment time among the genotypes. The CF parameter Fv/Fm was used to screen the 57 accessions, and the results showed clear differences in Fv/Fm between the susceptible and resistant genotypes. Susceptible genotypes had an especially low Fv/Fm value of less than 0.8, reflecting damage to the reaction center of photosystem II. It is concluded that Fv/Fm can be used as a CF parameter index for screening waterlogging stress tolerance in ginseng genotypes.

• Food Science and Preservation
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• v.18 no.4
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• pp.612-619
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• 2011

Cronobacter sakazakii has been isolated from a wide range of environmental sources and from several foods of animal and plant origin. The objective of this study was to determine the resistance of C. sakazakii (ATCC 12868, ATCC 29004, and ATCC 29544) in cold, cold-freeze thaw, cold-acid, and cold starvation-freeze thaw stress. The number of C. sakazakii decreased to 1 log CFU/mL at $5^{\circ}C$ (cold storage) for 10 days. When C. sakazakii was cultivated at a low temperature ($13^{\circ}C$), the population of C sakazakii ATCC 12868 and 29004 increased to $10^9$ CFU/mL, and the population of C. sakazakii ATCC 29544 increased to $10^8$ CFU/mL. For C. sakazakii ATCC 12868 and 29004, the cold-adapted cells ($5^{\circ}C$ 24 hr) decreased by 4 log CFU/mL, and the low-temperature-cultivated cells ($13^{\circ}C$) decreased by 0.5 log CFU/mL. In this study, low-temperature cultivation enhanced the freeze-thaw cross-resistance due to the metabolic changes in the cells. Cold stress ($5^{\circ}C$ 48 hr, $13^{\circ}C$ cultivation) enhanced the cold-acid cross-resistance. The cold-starved cells in the sterilized 0.1% peptone water enhanced the freeze-thaw cross-resistance with significant differences (p<0.05). Therefore, the increased tolerance of the cold-adapted or low-temperature-cultivated C. sakazakii cells to freeze-thaw, acid, or starvation suggests that such environments should be considered when processing minimally processed foods or foods with extended shelf life.

• Journal of fish pathology
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• v.10 no.2
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• pp.113-123
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• 1997

Concerned to the life cycle of Ichthyophthirius multifiliis, the biological characteristic of the parasites was studied in the rainbow trout (Oncorhynchus mykiss) and the Korean catfish (Silurus asotus) Under the experimental condition of $9^{\circ}C$- $28^{\circ}C$, tomitogenesis rate was positively proportional to water temperature, but not at over $28^{\circ}C$. The protomonts showed a high rate of tomitogenesis at $26^{\circ}C$ in comparision with other temperature conditions. Temperature affected tomitogenesis rate which resulted from the various conditions of salinity, pH and formalin concentration. The protomonts showed a high rate of tomitogenesis at pH 6.9 in comparision with other pH conditions at all temperatures tested. This result revealed that the opitimum pH for tomitogenesis was 6.9. The protomont had more tolerance against salinity and formalin concentration at low temperature ($18^{\circ}C$) than at high one ($22^{\circ}C$). Both trophont and protomont were not infective, but theront was infective.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.1
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• pp.159-170
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• 2013

The effect of climate change on urban woody plants remains difficult to predict in urban areas. Depending on its tolerances, a plant species may stay and survive or stay with slowly declining remnant populations under a changing climate. To predict those vulnerabilities on urban woody plants, this study suggests a basic bioclimatic envelop model of heat requirements, cold tolerance, chilling requirements and moisture requirements that are well documented as the 'climatic niche'. Each component of the 'climatic niche' is measured by the warmth index, the absolute minimum temperature, the number of chilling weeks and the water balance. Regarding the utility of the developed model, the selected urban plant's present probabilities are suggested in the future climate of Seoul. Both Korea and Japan's thermal thresholds are considered for a plant's optimal climatic niche. By considering the thermal thresholds of these two regions for the same species, the different responses observed will reflect the plant's 'hardening' process in a rising climate. The model illustrated that the subpolar plants Taxus cuspidata and Ulmus davidiana var. japonica are predicted to have low suitability in Seoul. The temperate plants Zelkova serrata and Pinus densiflora, which have a broad climatic niche, exhibited the highest present probability in the future. The subtropical plants Camellia japonica and Castanopsis cuspidata var. sieboldii may exhibit a modest growth pattern in the late 21C's future climatic period when an appropriate frost management scheme is offered. The model can be used to hypothesize how urban ecosystems could change over time. Moreover, the developed model can be used to establish selection guidelines for urban plants with high levels of climatic adaptability.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.13-13
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• 2022

Recently, stable crop production is threatened by the effects of climate change. In particular, it is difficult to consistently maintain agricultural policies due to large price fluctuations depending on the difference in total domestic rice production from year to year. For stable rice production amid changes in the crop growing environment, development of varieties with improved disease resistance and abiotic stress stability is becoming more important. In here, drought and cold tolerant trait have been studied. First, for the development of drought tolerant varieties, we analyzed which agricultural traits are mainly affected by domestic drought conditions. As a result, it was observed that drought caused by the lack of water during transplanting season inhibits the development of the number of tiller and reduces the yield. 'Samgang' was selected as a useful genetic resource with strong drought tolerant and stable tiller number development even under drought conditions by phenotype screening. Three of drought tolerant QTLs were identified using doubled haploid (DH) population derived from a cross between Nacdong and Samgang, a drought sensitive and a tolerant, respectively. Among these QTLs, when qVDT2 and qVDTl1 were integrated, it was investigated that the tiller number development was relatively stable in the rainfed paddy field conditions. It is known that the high-yielding Tongil-type cultivars are severely affected by cold stress throughout the entire growth stage. In this study, we established conditions that can test the cold tolerance phenotype with alternate temperature to treat low temperatures in indoor growth conditions similar to those in field conditions at seedling stage. Three cold tolerant QTLs were explored using population derived from a cross between Hanareum2 (cold sensitive variety, Tongil-type) and Unkwang (cold tolerant variety, Japonica). Among these QTLs, qSCT12 showed strong cold tolerant phenotype, and when all of three QTLs were integrated, it was investigated that cold tolerant score was relatively similar to its donor parent, Unkwang, in our experimental conditions. We are performing that development of new variety with improved cold tolerant through the introduction of these QTLs.