Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Effects of Foliar Application of Glycine Betaine on the Growth and Contents of Osmolyte in Tomato Seedling

Glycine betaine 엽면 처리가 토마토 유묘의 생육과 삼투조절물질 함량에 미치는 영향

  • 강남준 (원예연구소 시설원예시험장) ;
  • 권준국 (원예연구소 시설원예시험장) ;
  • 이재한 (원예연구소 시설원예시험장) ;
  • 박진면 (원예연구소 원예토양관리연구팀) ;
  • 이한철 (원예연구소 시설원예시험장) ;
  • 최영하 (원예연구소 시설원예시험장)
  • Published : 2006.12.30
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Abstract

Effects of exogenously foliar applied glycine betaine (GB) on the growth and contents of osmolyte in tomato seedling was investigated. Plants treated with exogenous glycine betaine induced better biomass production and plant height during chilling stress than the untreated plants. The total soluble sugar contents in GB foliar-applied plants lower than that of untreated plants 28 days after foliar application. Total water soluble protein contents in GB foliar-applied plants did not change 28 days after chilling stress. In untreated plant, it decreased rapidly in the beginning of chilling stress. Proline contents in untreated plants rapidly increased by the beginning of chilling stress, and then slightly decreased during the next 3 weeks. However proline contents in GB foliar-applied plants did not change during the 28 days chilling stress period. The results suggest that foliar application of GB is a effect methods to increase the chilling tolerance of tomato seedlings in protected cultivation system at low temperature season.

토마토 유묘의 생육에 미치는 glycine betaine의 엽면처리 효과를 분석한 결과, 초장이나 건물중 등의 생육이 대조구인 증류수 처리에 비해 촉진되는 경향을 보였으며 25mM glycine betaine 처리가 가장 좋았다. 저온스트레스 하에서 삼투조절 역할을 하는 가용성 당과 proline의 축적량이 증류수 처리에 비해 glycine betaine처리에서 낮은 경향을 보여 외부에서 엽면 처리한 glycine betaine이 삼투조절 역할을 한 것으로 판단되었으며 이러한 결과는 수용성 단백질과 유리 아미노산의 축적 양상에서도 잘 반영되었다. 이상의 결과로 보아 glycine betaine의 엽면 처리는 저온기 시설 토마토 재배 시 야간 저온장해를 극복할 수 있는 방법으로서 이용 가능성이 있을 것으로 사료된다.

Keywords

References

  1. Agboma, P.C., M.G.K. Jones, P. Peltonen-Sainio, H. Rita, and E. Pehu. 1997. Exogenous glycinebetaine enhances grain yield of maize, sorghum and wheat grown under two supplementary watering regimes. J. Agron. Crop Sci. 178:29-37 https://doi.org/10.1111/j.1439-037X.1997.tb00348.x
  2. Allard, F., M. Houde, M. Krol, A. Ivanov, N.P.A. Huner, and F. Sarhan. 1998. Betaine improves freezing tolerance in wheat. Plant Cell Physiol. 39:1194-1202 https://doi.org/10.1093/oxfordjournals.pcp.a029320
  3. Aspinall, D. and L.G. Paley. 1981. Proline accumulation: Physiological aspects, p. 206-242. In: L.G. Paley and D. Aspinall (eds.). The physiology and biochemical of drought resistance in plants. Academic Press, New York, NY, USA
  4. Bates, L.S. 1973. Rapid determination of free proline for water-stress studies. Plant Soil 39:205-207 https://doi.org/10.1007/BF00018060
  5. Bradford, M.M. 1976. A rapid and sensitive method for the quantitization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  6. Chen, W.P., P.H. Li, and T.H.H. Chen. 2000. Glycine betaine increases chilling tolerance and reduces chilling-induced lipid peroxidation in Zea mays L. Plant, Cell and Environment, 23:609-618 https://doi.org/10.1046/j.1365-3040.2000.00570.x
  7. Claussen, W. 2005. Proline as a measure of stress in tomato plants. Plant Science, 168:241-248 https://doi.org/10.1016/j.plantsci.2004.07.039
  8. Gorham, J. 1995. Betaines in higher plants-biosynthesis and role in stress metabolism, p. 172-203. In: R.M. Wallsgrove (ed.). Amino acids and their derivatives in higher plants. University Press, Cambridge, UK
  9. Hahn, M. and V. Walbot. 1989. Effects of cold-treatment on protein synthesis and mRNA levels in rice leaves. Plant Physiol. 91:930-938 https://doi.org/10.1104/pp.91.3.930
  10. Heuer, B. 2003. Influence of exogenous application of proline and glycine betaine on growth of salt-stressed tomato plants. Plant Science 165:693-699 https://doi.org/10.1016/S0168-9452(03)00222-X
  11. Kishitani, S., K. Watanabe, S. Yasuda, K. Arakawa, and T. Takabe. 1994. Accumulation of glycine betaine during cold acclimation and freezing tolerance in leaves of winter and spring barley plants. Plant Cell Environ. 17:89-95 https://doi.org/10.1111/j.1365-3040.1994.tb00269.x
  12. Koster, K.L. and D.V. Lynch. 1992. Solute accumulation and compartmentation during the cold acclimation of Puma rye. Plant Physiol. 98:108-113 https://doi.org/10.1104/pp.98.1.108
  13. Makela, P., J. Mantila, R. Hinkkanen, E. Pehu, and P. Peltonen-Sainio. 1996a. Effect of foliar application of glycine betaine on stress tolerance, growth, and yield of spring cereals and summer turnip rape in Finland. J. Agron. Crop Sci. 176:223-234 https://doi.org/10.1111/j.1439-037X.1996.tb00467.x
  14. Makela, P., P. Peltonen-Sainio, K. Jokinen, P. Pehu, H. Setala, R. Hinkkanen, and S. Somersalo. 1996b. Uptake and translocation of foliar-applied glycinebetaine in crop plants. Plant Science 121:221-230 https://doi.org/10.1016/S0168-9452(96)04527-X
  15. Murata, M., P.S. Mohanty, H. Hayashi, and G.C. Papageorgiou. 1992. Glycine betaine stabilizes the association of extrinsic proteins with the photosynthetic oxygen-evolving complex. FEBS Lett. 296:187-189 https://doi.org/10.1016/0014-5793(92)80376-R
  16. Naidu, B.P., L.G. Paley, D. Aspinall, A.C. Jennings, and G.P. Jones. 1991. Amino acid and glycine betaine accumulation in cold-stressed wheat seedlings. Phytochemistry 30:407-409 https://doi.org/10.1016/0031-9422(91)83693-F
  17. Nelson, N. 1944. A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 153:375-380
  18. Perras, M. and F. Sarham. 1984. Energy state of spring and winter wheat during cold hardening. Soluble sugars and adenine nucleotides. Physiol. Plant. 60:129-132 https://doi.org/10.1111/j.1399-3054.1984.tb04552.x
  19. Rajashekar, C.B. 2000. Cold response and freezing tolerance in plants, p. 321-341. In: R.E. Wilkinson (ed.). Plant-environment interactions. Marcel Dekker Press, New York, NY, USA
  20. Rajashekar, C.B., H. Zhou, K.B. Marcum, and O. Prakash. 1999. Glycine betaine accumulation and induction of cold tolerance in strawberry (Fragaria ${\times}$ ananassa Duch.) plants. Plant Sci. 148:175-183 https://doi.org/10.1016/S0168-9452(99)00136-3
  21. Rhodes, D. and A.D. Hanson. 1993. Quarternary ammonium and tertiary sulfonium compounds in higher plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 44:357-384 https://doi.org/10.1146/annurev.pp.44.060193.002041
  22. Rudolph, A.S. and J.H. Crowe. 1985. Membrane stabilization during freezing: The role of two natural cryprotectants, trehalose and proline. Cryobiology 22:367-377 https://doi.org/10.1016/0011-2240(85)90184-1
  23. Storey, R. and R.G. Wyn Jones. 1977. Quaternary ammonium compounds in plants in relation to salt resistance. Phytochemistry 16:447-453 https://doi.org/10.1016/S0031-9422(00)94326-7
  24. Xing, W. and C.B. Rajashekar. 1999. Alleviation of water stress in beans by exogenous glycine betaine. Plant Sci. 148:185-195 https://doi.org/10.1016/S0168-9452(99)00137-5
  25. Yemm, E.W. and E.C. Cocking. 1955. The determination of amino acids with ninhydrin. Analyst. 80:209-264 https://doi.org/10.1039/an9558000209