• Title/Summary/Keyword: Localization Development

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Localization of Manduca sexta Allatotropin Neuropeptides in Developing Ventral Nerve Cord of the Silk Moth Bombyx mori

  • Park, Cheolin;Lee, Bong-Hee
    • International Journal of Industrial Entomology and Biomaterials
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    • v.3 no.2
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    • pp.177-185
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    • 2001
  • This study has been carried out to determine localization of Manduca sexta allatotropin (Mas-AT) neuropeptide in developing ventral nerve cord of the silk moth Bombyx mori with polyclonal antisera against Mas-AT. Suboesophageal ganglion (SOG) of the second to fifth instar larvae and 3-day-old pupae showed two to ten Mas-AT-immunoreactive (Mas-AT-IR) cell bodies. There were two to three pairs of labeled cell bodies in each thoracic ganglion (TG) from third instar larvae to adults, with the exception of TG from prepupae. One pair of labeled cell bodies was localized in each abdominal ganglion (AG) 1 to 6 from third instar larvae to 3-day-old pupae, whereas in 5-day-old pupae to adults there was one pair in a similar location of AG 1 to 5. The seventh neuromeres of terminal abdominal ganglia (TAG) from third instar Iarvae to 3- day-old pupae contained four labeled large cell bodies. In each of AG 1 to 7, these cell bodies showed similar allatotropin-immunoreactivity in appearance. Some labeled axons, projected from Mas-AT-lR cells in each of those AG, were extended to the nerves N 1 and N 2.

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Concurrent Mapping and Localization using Range Sonar in Small AUV, SNUUVI

  • Hwang Arom;Seong Woojae;Choi Hang Soon;Lee Kyu Yuel
    • Journal of Ship and Ocean Technology
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    • v.9 no.4
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    • pp.23-34
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    • 2005
  • Increased usage of AUVs has led to the development of alternative navigational methods that use the acoustic beacons and dead reckoning. This paper describes a concurrent mapping and localization (CML) scheme that uses range sonars mounted on SNUUV­I, which is a small test AUV developed by Seoul National University. The CML is one of such alternative navigation methods for measuring the environment that the vehicle is passing through. In addition, it is intended to provide relative position of AUV by processing the data from sonar measurements. A technique for CML algorithm which uses several ranging sonars is presented. This technique utilizes an extended Kalman filter to estimate the location of the AUV. In order for the algorithm to work efficiently, the nearest neighbor standard filter is introduced as the algorithm of data association in the CML for associating the stored targets the sonar returns at each time step. The proposed CML algorithm is tested by simulations under various conditions. Experiments in a towing tank for one dimensional navigation are conducted and the results are presented. The results of the simulation and experiment show that the proposed CML algorithm is capable of estimating the position of the vehicle and the object and demonstrates that the algorithm will perform well in the real environment.

A Research on Development of Applique Armor Kit for Design of Manufacturing Technology based on the Reverse Engineering (역설계 기반 제조기술 설계를 통한 방탄용 부가장갑킷트 개발에 관한 연구)

  • Ko, Dong Hyeon;Moon, Tae Sang;Kim, Young Ki
    • Journal of Korean Society for Quality Management
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    • v.49 no.3
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    • pp.233-243
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    • 2021
  • Purpose: The study focused on localization of the enhanced applique armor kit (EAAK), which are applied to Korea Assault Amphibious Vehicle (KAAV). Methods: For this propose, we developed the manufacturing technology by analyzing the original products. Considering the conditions used in the military, we made the test evaluation criteria. Results: In results, The EAAK developed through this research performs equivalent performance compared to Rafael, Co. Thus, It is possible not only stably supply a parts of EAAK for maintenance, but also to reduce costs due to import substitution effect. Conclusion: It is expected that the manufacturing technology and test evaluation criteria accumulated through this study can be used in the localization of similar parts.

Distributed Target Localization with Inaccurate Collaborative Sensors in Multipath Environments

  • Feng, Yuan;Yan, Qinsiwei;Tseng, Po-Hsuan;Hao, Ganlin;Wu, Nan
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.13 no.5
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    • pp.2299-2318
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    • 2019
  • Location-aware networks are of great importance for both civil lives and military applications. Methods based on line-of-sight (LOS) measurements suffer sever performance loss in harsh environments such as indoor scenarios, where sensors can receive both LOS and non-line-of-sight (NLOS) measurements. In this paper, we propose a data association (DA) process based on the expectation maximization (EM) algorithm, which enables us to exploit multipath components (MPCs). By setting the mapping relationship between the measurements and scatters as a latent variable, coefficients of the Gaussian mixture model are estimated. Moreover, considering the misalignment of sensor position, we propose a space-alternating generalized expectation maximization (SAGE)-based algorithms to jointly update the target localization and sensor position information. A two dimensional (2-D) circularly symmetric Gaussian distribution is employed to approximate the probability density function of the sensor's position uncertainty via the minimization of the Kullback-Leibler divergence (KLD), which enables us to calculate the expectation step with low computational complexity. Moreover, a distributed implementation is derived based on the average consensus method to improve the scalability of the proposed algorithm. Simulation results demonstrate that the proposed centralized and distributed algorithms can perform close to the Monte Carlo-based method with much lower communication overhead and computational complexity.

Sub-Ciliary Segregation of Two Drosophila Transient Receptor Potential Channels Begins at the Initial Stage of Their Pre-Ciliary Trafficking

  • Kwon, Youngtae;Lee, Jeongmi;Chung, Yun Doo
    • Molecules and Cells
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    • v.43 no.12
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    • pp.1002-1010
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    • 2020
  • Cilia are important eukaryotic cellular compartments required for diverse biological functions. Recent studies have revealed that protein targeting into the proper ciliary subcompartments is essential for ciliary function. In Drosophila chordotonal cilium, where mechano-electric transduction occurs, two transient receptor potential (TRP) superfamily ion channels, TRPV and TRPN, are restricted to the proximal and distal subcompartments, respectively. To understand the mechanisms underlying the sub-ciliary segregation of the two TRPs, we analyzed their localization under various conditions. In developing chordotonal cilia, TRPN was directly targeted to the ciliary tip from the beginning of its appearance and was retained in the distal subcompartment throughout development, whereas the ciliary localization of TRPV was considerably delayed. Lack of intraflagella transport-related proteins affected TRPV from the initial stage of its pre-ciliary trafficking, whereas it affected TRPN from the ciliary entry stage. The ectopic expression of the two TRP channels in both ciliated and non-ciliated cells revealed their intrinsic properties related to their localization. Taken together, our results suggest that sub-ciliary segregation of the two TRP channels relies on their distinct intrinsic properties, and begins at the initial stage of their pre-ciliary trafficking.

An Approach to the Localization of Technology for a Transport and Storage Container for Very Low-Level Radioactive Liquid Waste

  • Shin, Seung Hun;Choi, Woo Nyun;Yoon, Seungbin;Lee, Un Jang;Park, Hye Min;Kim, Hee Reyoung
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.20 no.1
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    • pp.127-131
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    • 2022
  • The structural safety of prototype transport and storage containers for very low-level radioactive liquid waste was experimentally estimated for its localization development. Transport containers for radioactive liquid waste have been researched and developed, however, there are no standardized commercial containers for very low-level radioactive waste in Korea. In this study, the structural safety of the designated IP-2 type container capable of transporting and temporarily storing large amounts of very low-level liquid waste, which is generated during the operation and decommissioning of nuclear power plants, was demonstrated. The stacking and drop tests, which were conducted to determine the structural integrity of the container, verified that there was no external leakage of the contents in spite of its structural deformation due to the drop impact. This study shows the effort required for the localization of the technology used in manufacturing transport and storage containers for very low-level radioactive liquid waste, and the additional structural reinforcement of the container in which the commercial intermediate bulk container (IBC) external frame was coupled.

A Model Stacking Algorithm for Indoor Positioning System using WiFi Fingerprinting

  • JinQuan Wang;YiJun Wang;GuangWen Liu;GuiFen Chen
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.4
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    • pp.1200-1215
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    • 2023
  • With the development of IoT and artificial intelligence, location-based services are getting more and more attention. For solving the current problem that indoor positioning error is large and generalization is poor, this paper proposes a Model Stacking Algorithm for Indoor Positioning System using WiFi fingerprinting. Firstly, we adopt a model stacking method based on Bayesian optimization to predict the location of indoor targets to improve indoor localization accuracy and model generalization. Secondly, Taking the predicted position based on model stacking as the observation value of particle filter, collaborative particle filter localization based on model stacking algorithm is realized. The experimental results show that the algorithm can control the position error within 2m, which is superior to KNN, GBDT, Xgboost, LightGBM, RF. The location accuracy of the fusion particle filter algorithm is improved by 31%, and the predicted trajectory is close to the real trajectory. The algorithm can also adapt to the application scenarios with fewer wireless access points.

Analysis of Localization Technology Performance Based on Accumulated RSSI Signal Using Simulation (시뮬레이션을 이용한 누적 RSSI 신호 기반의 항법 기술 성능 분석)

  • Beomju Shin;Taikjin Lee
    • Journal of Positioning, Navigation, and Timing
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    • v.13 no.3
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    • pp.331-339
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    • 2024
  • Reliable and precise indoor localization is crucial for personal navigation, emergency rescue, and monitoring workers indoors. To use this technology in different applications, it is important to make it less dependent on infrastructure and to keep the error as small as possible. Fingerprinting stands out as a popular choice for indoor positioning because it leverages existing infrastructure and works with just a smartphone. However, its accuracy heavily relies on the quality of that infrastructure. For instance, having too few access points or beacons can greatly reduce its effectiveness. To reduce dependence on RF infrastructure, we have developed surface correlation (SC) using accumulated Received Signal Strength Indicator (RSSI) signals This approach constructs a user mask for radio map comparisons using an accumulated RSSI vector and the trajectory of the user, which is estimated through PDR. The location with the highest correlation is considered as the user's position after comparison. Through a simulation, the performance of short RSSI vector-based technology and SC is analyzed, and future directions for the development of SC are discussed.

Differential expression and localization of tight junction proteins in the goat epididymis

  • Sung Woo, Kim;Yu-Da, Jeong;Ga-Yeong, Lee;Jinwook, Lee;Jae-Yeung, Lee;Chan-Lan, Kim;Yeoung-Gyu, Ko;Sung-Soo, Lee;Bongki, Kim
    • Journal of Animal Science and Technology
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    • v.64 no.3
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    • pp.500-514
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    • 2022
  • The blood-epididymis barrier (BEB) forms a unique microenvironment that is crucial for the maturation, protection, transport, and storage of spermatozoa in the epididymis. To characterize the function of tight junctions (TJs), which are constitutive components of the BEB, we determined the expression and localization of TJ proteins such as zonula occludens (ZO)-1, 2, and 3, occludin, and claudin3 (Cldn3) during postnatal development in the goat epididymis. To assess the expression patterns of TJ proteins in immature (3 months of age) and mature (14 months of age) goat epididymides, two different experimental methods were used including immunofluorescence labeling and western blotting. We show that, ZO-1, 2, and 3, and occludin, were strictly expressed and localized to the TJs of the goat epididymis, whereas Cldn3 was present in basolateral membranes as well as TJs. All TJ proteins examined were more highly expressed in the immature epididymis compared to levels in mature tissue. In conclusion, our study indicates that at least five TJ proteins, namely ZO-1, ZO-2, ZO-3, occludin, and Cldn3, are present in TJs, and the expression strength and pattern of TJ proteins tend to be age dependent in the goat epididymis. Together, these data suggest that the distinct expression patterns of TJ proteins are essential for regulating components of the luminal contents in the epididymal epithelium and for forming adequate luminal conditions that are necessary for the maturation, protection, transport, and storage of spermatozoa in the goat epididymis.

Analysis on the Contribution of FDOA Measurement Accuracy to the Performance of Combined TDOA/FDOA Localization Systems (TDOA/FDOA 복합 위치추정 시스템에서 FDOA 측정 정확도에 따른 추정 성능 기여도 분석)

  • Kim, Dong-Gyu;Kim, Yong-Hee;Han, Jin-Woo;Song, Kyu-Ha;Kim, Hyoung-Nam
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.5
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    • pp.88-96
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    • 2014
  • In modern electronic warfare systems, the necessity of a more accurate estimation method based on non-AOA (arrival of angle) measurement, such as TDOA and FDOA, have been increased. The previous researches using single TDOA have been carried out in terms of not only the development of emitter location algorithms but also the enhancement of measurement accuracy. Recently, however, the combined TDOA/FDOA method is of considerable interest because it is able to estimate the velocity vector of a moving emitter and acquire a pair of TDOA and FDOA measurements from a single sensor pair. In this circumstance, it is needed to derive the required FDOA measurement accuracy in order that the TDOA/FDOA combined localization system outperforms the previous single TDOA localization systems. Therefore, we analyze the contribution of FDOA measurement accuracy to emitter location, then propose the criterion based on CRLB (Cramer-Rao lower bound). Simulations are included to examine the validity of the proposed criterion by using the Gauss-Newton algorithm.