• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.582-585
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• 2009

Bovine viral diarrhea virus (BVDV) is an economically important worldwide disease in livestock industry. In this study, the occurrence of BVDV in Korean indigenous cattle was performed by RT-PCR using nasal swab. Twelve of 21 cattle were identified as BVDV positive and classified as persistently infected (PI). These animals showed the occurrence of diseases such as diarrhea and pneumonia. BVDV PI outbreaks were found mostly in PI calves. Sequencing and phylogenetic analysis based on the 5'-untranslated region (UTR) showed that our case belonged to BVDV-2a. These results suggested that the nasal swab sampling was available method for the detection of PI animals, underscoring the need for BVDV control strategies in Korean indigenous cattle.

• Korean Journal of Veterinary Research
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• v.45 no.2
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• pp.287-296
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• 2005

This experiments was designed to observe the effect of period and dose of ingestion of grapes on renal failure in dog. After the dogs had been mixed of general feed, raisins from U.S.A and fresh grapes from Chile selling in Korea. We observed the clinical sign, and analyzed the blood and urine using by biochemical test. The experiment was executed by the two following groups. The experiment group 1. derived renal failure by supplying the mixed general feed with fresh grapes and dry grapes 15 g per weight kg, 2 times a day (AM 09, PM 21), the experiment group 2. executed to derive renal failure by supplying the mixed general feed with fresh grapes and dry grapes 40 g per weight kg, 2 times a day. Extraction of blood for analysis was conducted one time a day and clinical test for renal failure was executed by means of a blood analysis, biochemical analysis, urine analysis, excretory urography (E.U) and E.R.D-$screen^{TM}$ urine test (Heska, USA). The results of group 1 were normal ranges (BUN 9.0~22.6 mg/dl, creatinine 0.8~1.2 mg/dl, Ca 9.7~12.3 mg/dl, Pi 2.9~4.6 mg/dl), renal failure was not observed. On the 3rd day in group 2, azotemia was arisen from the increasing BUN 83 mg/dl (7~25 mg/dl), creatinine 2.3 mg/dl (0.5~1.4 mg/dl), when executed urine was tested by E.R.D-$screen^{TM}$ test using in the early kidney disease diagnosis, microalbumine state was high positive, and it showed stale delay by using excretory urography (EU). This study demonstrated that acute renal failure by grapes and raisins dependent on food dose, and specific characters of individual.

• Korean Journal of Veterinary Service
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• v.35 no.2
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• pp.133-137
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• 2012

Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise sero-prevalence was found 3.0% in 2~3 years age group and 2.0% in 4~8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

• Journal of the Korean Data and Information Science Society
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• v.24 no.1
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• pp.151-159
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• 2013

It is well known that disease of human and economic traits of livestock are affected a lot by gene combination effect rather than a single gene effect. But existing methods have disadvantages such as heavy computing, many expenses and long time. In order to overcome those drawbacks, SNPHarvester was developed to find the main gene combinations among the many genes. In this paper, we used the superior gene combination which are related to the quality of the Korean beef cattle among sets of SNPs by SNPHarvester, and identified the superior genotypes using radial graph which can enhance various qualities of Korean beef among selected SNP combinations.

• Journal of Embryo Transfer
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• v.30 no.3
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• pp.257-260
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• 2015

Reproductive disorders in cows cause economic loss in livestock farms. Reproductive diseases, such as follicular cyst, luteal cyst, endometritis, pyometra, and repeat breeding cause infertility. Among these diseases, endometritis and pyometra are uterine infections that are leading causes of infertility. This study was performed to investigate the causative agents of uterine diseases using bacterial culture. Bacteria were obtained from the reproductive organs (vagina, uterine cervix, and uterine horn) of dairy cow diagnosed with endometritis or pyometra, and cultured on blood agar. The colonies obtained from cultivation for 24 hours were passaged. To identify the bacteria, the colonies grown in passaged culture Gram stained and applied to an automatic biochemical microbial identification system. Escherichia coli were commonly detected in vagina, uterine cervix, and uterine horn of dairy cows diagnosed to pyometra. The cows having endometritis showed not only Escherichia coli but also Pantoea spp. and Klebsiella spp. strains. Dairy cows that were infected with Escherichia coli in uterus caused mastitis or digestive disease. These results suggest that sanitary feeding and management beforehand are needed to prevent bacterial infections.

• Parasites, Hosts and Diseases
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• v.59 no.5
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• pp.457-464
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• 2021

Cystic echinococcosis is a zoonotic parasitic disease caused by Echinococcus species. Tanzania is one of the endemic countries with cystic echinococcosis. This study focussed on identifying genotypes of Echinococcus spp. in Tanzania. We collected 7 cysts from cattle in Mwanza municipal (n=4) and Loliondo district (n=3). The cysts from Mwanza were all E. ortleppi and fertile. In contrast, the cysts from Loliondo were all E. granulosus sensu stricto and sterile. Two from the 4 cysts were a new haplotype of E. ortleppi (G5). These results can improve the preventive and control programs for humans and livestock in Tanzania. To our knowledge, this study is considered the first to identify the genotype and haplotype of Echinococcus spp. in Tanzania.

• Journal of Animal Science and Technology
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• v.63 no.4
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• pp.854-863
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• 2021

Weaning induces physiological changes in intestinal development that affect pigs' growth performance and susceptibility to disease. As a posttranscriptional regulator, microRNAs (miRNAs) regulate cellular homeostasis during intestinal development. We performed small RNA expression profiling in the small intestine of piglets before weaning (BW), 1 week after weaning (1W), and 2 weeks after weaning (2W) to identify weaning-associated differentially expressed miRNAs. We identified 38 differentially expressed miRNAs with varying expression levels among BW, 1W, and 2W. Then, we classified expression patterns of the identified miRNAs into four types. ssc-miR-196a and ssc-miR-451 represent pattern 1, which had an increased expression at 1W and a decreased expression at 2W. ssc-miR-499-5p represents pattern 2, which had an increased expression at 1W and a stable expression at 2W. ssc-miR-7135-3p and ssc-miR-144 represent pattern 3, which had a stable expression at 1W and a decreased expression at 2W. Eleven miRNAs (ssc-miR-542-3p, ssc-miR-214, ssc-miR-758, ssc-miR-4331, ssc-miR-105-1, ssc-miR-1285, ssc-miR-10a-5p, ssc-miR-4332, ssc-miR-503, ssc-miR-6782-3p, and ssc-miR-424-5p) represent pattern 4, which had a decreased expression at 1W and a stable expression at 2W. Moreover, we identified 133 candidate targets for miR-196a using a target prediction database. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the target genes were associated with 19 biological processes, 4 cellular components, 8 molecular functions, and 7 KEGG pathways, including anterior/posterior pattern specification as well as the cancer, PI3K-Akt, MAPK, GnRH, and neurotrophin signaling pathways. These findings suggest that miRNAs regulate the development of the small intestine during the weaning process in piglets by anterior/posterior pattern specification as well as the cancer, PI3K-Akt, MAPK, GnRH, and neurotrophin signaling pathways.

• Journal of Animal Science and Technology
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• v.63 no.3
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• pp.465-474
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• 2021

It has become important to explore alternative feed ingredients to reduce feed costs, which are burdensome for livestock production. In addition, it is desirable to find efficient and functional alternative ingredients for traditional feed ingredients in pig diets, considering the stress and sensitivity of disease of pig. Rice is produced around the world like corn that is the typical energy source in pig diets. Although the nutritional quality varies depending on the degree of milling, rice, except whole grains (paddy rice), contains more starch than corn and its structure and granule size are easier to digest than corn. In addition, the fact that rice has fewer non-starch polysaccharides (NSP) and anti-nutritional factors (ANFs) is also effective in improving digestibility and various polyphenols in rice can help modulation of immune responses, which can be beneficial to the gastrointestinal environment and health of pig. Many studies have been conducted on rice focusing on things such as degree of milling, substitution rates of corn, granule size, and processing methods. Most results have shown that rice can be partially or completely used to replace corn in pig diets without negatively affecting pig growth and production. While further research should focus on the precise biological mechanisms at play, it was confirmed that the use of rice could reduce the use of antibiotics and pig removal and protect pigs from gastrointestinal diseases including diarrhea. From this point of view, rice can be evaluated as a valuable feed ingredient for swine diets.

• Journal of The Korean Society of Agricultural Engineers
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• v.61 no.5
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• pp.101-108
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• 2019

The domestic duck industry is the sixth-largest among the livestock industries. However, 34.3% of duck houses were the duck houses arbitrarily converted from plastic greenhouses. This type of duck house was difficult to properly manage internal air temperature and humidity environment. Humidity environment inside duck houses is an important factor that directly affects the productivity and disease occurrence of the duck. Although the humidity environments of litters (bedding materials) affect directly the inside environment of duck houses, there are only few studies related to humidity environment of litters. In this study, evaporation rates from litters were evaluated according to air temperature, relative humidity, water contents of litters, and wind speed. The experimental chamber was made to measure evaporation rates from litters. Temperature and humidity controlled chamber was utilized during the conduct of the laboratory experiments. Using the measured data, a multi linear regression analysis was carried out to derive the calculation formula of evaporation rates from litters. In order to improve the accuracy of the multi linear regression model, the partial vapor pressure directly related to evaporation was also considered. Variance inflation factors of air temperature, relative humidity, partial vapor pressure, water contents of litters, and wind speed were calculated to identify multicollinearity problem. The Multiple $R^2$ and adjusted-$R^2$ of regression model were calculated at 0.76 and 0.71, respectively. Therefore, the regression models were developed in this study can be used to estimate evaporation rates from the litter of duck houses.

• Korean Journal of Veterinary Service
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• v.44 no.1
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• pp.27-33
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• 2021

Q fever is a worldwide zoonotic disease caused by Coxiella burnetii. Domestic ruminants are considered to be major source of human infection. The aim of this survey was to investigate seroprevalence of C. burnetii in ruminants in Gwangju area. A total of 1,000 samples (serum and lactoserum) were collected from 987 Korean native cattle, 5 Korean native goats, 2 beef cattle, 6 bulk-tank milk from each dairy farm in Gwangju area from January to October 2020 and analyzed by ELISA. The seroprevalence of C. burnetii in bulk-tank milk from each dairy farms was 50.0%. Korean black goat and beef cattle had negative antibody test results for C. burnetii. The seroprevalence of C. burnetii in Korean native cattle in Gwangju area was 7.1% and was higher in female (7.8%) than in male (3.4%) (P=0.024). The seroprevalence of C. burnetii in Korean native cattle appeared to increase with age (3.8% in 1 year-old, 7.1% in 3 year-old, and 10.7% in more than 5 year-old) (P<0.001). The seroprevalence of C. burnetii of Korean native cattle increased in spring and May was the highest in particular (P<0.001). As the distribution and density of tick-habitat are expected to increase due to climate crisis, this survey highlights the need for monitoring C. burnetii in domestic ruminants, including surveillance of C. burnetii infection in people working for livestock industry.