Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Case of Bacterial Identification in Reproductive Organs of Holstein Dairy Cows with Endometritis or Pyometra

자궁내막염 혹은 자궁축농증 발병 젖소의 생식기 내 분포하는 세균 동정 증례

  • 최창용 (농촌진흥청 국립축산과학원) ;
  • 정영훈 (농촌진흥청 국립축산과학원) ;
  • 조용일 (농촌진흥청 국립축산과학원) ;
  • 류재규 (농촌진흥청 국립축산과학원) ;
  • 임석기 (농촌진흥청 국립축산과학원) ;
  • 권응기 (농촌진흥청 국립축산과학원) ;
  • 성환후 (농촌진흥청 국립축산과학원) ;
  • 김성우 (농촌진흥청 국립축산과학원) ;
  • 조영무 (농촌진흥청 국립축산과학원) ;
  • 김창운 (경상대학교 의과대학 생리학교실) ;
  • 강다원 (경상대학교 의과대학 생리학교실)
  • Received : 2015.07.21
  • Accepted : 2015.09.08
  • Published : 2015.09.30
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Abstract

Reproductive disorders in cows cause economic loss in livestock farms. Reproductive diseases, such as follicular cyst, luteal cyst, endometritis, pyometra, and repeat breeding cause infertility. Among these diseases, endometritis and pyometra are uterine infections that are leading causes of infertility. This study was performed to investigate the causative agents of uterine diseases using bacterial culture. Bacteria were obtained from the reproductive organs (vagina, uterine cervix, and uterine horn) of dairy cow diagnosed with endometritis or pyometra, and cultured on blood agar. The colonies obtained from cultivation for 24 hours were passaged. To identify the bacteria, the colonies grown in passaged culture Gram stained and applied to an automatic biochemical microbial identification system. Escherichia coli were commonly detected in vagina, uterine cervix, and uterine horn of dairy cows diagnosed to pyometra. The cows having endometritis showed not only Escherichia coli but also Pantoea spp. and Klebsiella spp. strains. Dairy cows that were infected with Escherichia coli in uterus caused mastitis or digestive disease. These results suggest that sanitary feeding and management beforehand are needed to prevent bacterial infections.

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References

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