• Asian-Australasian Journal of Animal Sciences
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• 제18권1호
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• pp.3-7
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• 2005

The insulin-like growth factors (IGFs), their receptors, and their binding proteins play key roles in regulating cell proliferation and apoptosis. Insulin-like growth factor binding protein-3 (IGFBP3, OMIM #146732) is one of the proteins that bind to the IGFs. IGFBP3 is a modulator of IGF bioactivity, and direct growth inhibitor in the extravascular tissue compartment. We identified twenty-two novel single nucleotide polymorphisms (SNPs) in IGFBP3 gene in Korean cattle (Hanwoo, Bos taurus coreanae) by direct sequencing of full gene including -1,500 bp promoter region. Among the identified SNPs, five common SNPs were screened in 650 Korean cattle; one SNP in promoter (IGFBP3 G-854C), one in 5'UTR region (IGFBP3 G-100A), two in intron 1 (IGFBP3 G+421T, IGFBP3 T+1636A), and one in intron 2 (IGFBP3 C+3863A). The frequencies of each SNP were 0.357 (IGFBP3 G-854C), 0.472 (IGFBP3 G-100A), 0.418 (IGFBP3 G+421T), 0.363 (IGFBP3 T+1636A) and 0.226 (IGFBP3 C+3863A), respectively. Haplotypes and their frequencies were estimated by EM algorithm. Six haplotypes were constructed with five SNPs and linkage disequilibrium coefficients (|D'|) between SNP pairs were also calculated. The information on SNPs and haplotypes in IGFBP3 gene could be useful for genetic studies of this gene.

• 대한지리학회지
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• 제38권1호
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• pp.104-126
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• 2003

본 연구는 농협 농산물 물류센터의 상적, 물적 유통체계를 파악하고, 물류의 효율성과 집약화를 위해 설립된 청주 물류센터의 계절별 농$.$축$.$수산물의 입하지를 고찰하고자 한다. 그리고 각 상품의 판매액에 의한 배송의 지역유형과 지역특성과의 관계를 밝히는 것을 연구목적으로 한다. 그 결과 청주 물류센터는 충북을 핵심지역으로 전국에서 농$.$축$.$수산물을 입하하여 충북과 충남, 대전시 일부 지역에 배송을 집중화시키고 있다. 이는 청주 물류센터가 취급하고 있는 농$.$축$.$수산물이 신선도를 요하는 상품이기 때문에 물류센터에서 상대적으로 가까운 지역에서 주로 입하하여 근거리 배송을 하는 지역 물류시스템을 형성하고 있다는 것이다. 이러한 농$.$축$.$수산물의 배송은 수급관계라는 경제원칙뿐만 아니라 물류센터의 판매전략도 크게 작용하고 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권7호
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• pp.1022-1028
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• 2005

The objectives of this study were to evaluate and compare the environmental load of two different concentrate feed supply systems to the Japanese domestic livestock industry using the Life-cycle Assessment (LCA) method. The current system was defined as that requiring 11.469 million tons of corn imported from the US by sea transport and supplied as concentrate feed to the Japanese domestic livestock industry. The new system proposed by Kaku et al. in 2004 was defined as where 802,830 tons of US imported corn would not be planted in US and would be replaced by barley planted in 278 thousand ha of Japanese domestic land left fallow for the past year. In this case, 909,000 tons of domestic harvest barley would have been supplied as concentrate feed to the Japanese domestic livestock industry in 2000. The activities taken into account within the two system boundaries were three stages: concentrate feed production, feed transportation and gas emission from the soil by chemical fertilizer. Finished compost was regarded as organic fertilizer and was put instead of chemical fertilizers within the system boundary. Adoption of this new concentrate feed supply system by the Japanese domestic livestock industry could reduce 78,462 tons $CO_2$-equivalents of global warming potential, 347 tons $SO_2$-equivalents of acidification potential, 54 tons $PO_4$-equivalents of eutrophication potential and 0.842 million GJ as energy consumption below 2,000 levels. This LCA study comparing two Japanese domestic livestock concentrate feed supply systems showed that the stage of feed transport contributed most to global warming and the stage of emission from the soil contributed most to acidification and eutrophication. The Japanese domestic livestock industry could participate in emissions trading with $CO_2$-equivalents reduced by shifting from some imported US corn as a concentrate feed to domestic barley planted in land left fallow. In that case the Japanese government could launch emissions trading in accordance with Kyoto Protocol in the future.

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 2005년도 학술심포지엄, 제43회 학술발표회
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• pp.162-163
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• 2005

• Asian-Australasian Journal of Animal Sciences
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• 제12권5호
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• pp.689-694
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• 1999

This experiment was carried out to isolate the partial bovine (Bos Taurus coreanae) myogenic factor encoding gene, MyoD, using the rat myogenic factor (MyoD) gene sequence and to compare the gene sequence between another myogenic factor (Myf 5) and MyoD gene of the bovine. To make the probe and isolate the MyoD gene, PCR was performed to amplify rat and bovine MyoD gene including exon I, II and intron I. The homology between mouse and bovine MyoD is high; bovine MyoD gene shows 17 different gene sequence region compared to rat MyoD. Among those, two regions have significant differences; one is the exon I part between 2834 and 2850 bp, the other is intron part between 3274 and 3303 bp of the mouse. At this region homology was 40% in the former and 50% in the latter. Homology between bovine MyoD and Myf5 was 83% in the exon 1. Especially exon I in the Myf5 602-617 bp and 651-683 bp have significant differences. These results suggest that MyoD gene have a similar gene structure in mouse and bovine and MyoD and Myf5 of the bovine, at least in part, have a similar expression and activity.

• 한국동물위생학회지
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• 제32권2호
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• pp.119-124
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• 2009

The epidemiological survey of mass outbreak region of bovine tuberculosis from January of 2007 through May in 2009. The results were enumerated as follows. The results of tuberculin skin test are: 7 (0.4%) out of 1,697 in 2007, 61 (2.8%) out of 2,163 in 2008, 80 (4.9%) out of 1,639 in 2009. The sex and age distribution among the incidence of positive: 135 (91.8%) out of 147 in female, 12 (8.2%) in male. Among female, age 1: 6.1%, age 2: 30.6%, 3: 38.8%, 4: 14.2%, 5: 0.7% and 6: 1.4%. Among male, age 1: 4.1%, 2: 1.4%, 3: 2.7% and more frequent occurrence in age 3, 38.8% in female and 2.7% in male. The rate of recurrence by farms: recurrence 1: 6 (35.3%), 2: 9 (52.9%), 3: 1 (5.9%), 6: 1 (5.9%), The recurrence rate of 2 or more was 64.7%. The ELISA test result among 114 heads over 14 farms: 75 (65.8%) showed positive and 39 (34.2%) negative. Geographical distribution of recurrence is characterized as concentrated along the major traffic and stream crossing the village, and spread from the high elevation to downward area.

• 한국동물위생학회지
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• 제34권3호
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• pp.195-200
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• 2011

Swine influenza is an acute respiratory disease prevalent in pig-growing areas all around the world and plays the roles of an intermediate host to be transmitted to mammals including human beings through a genetic recombination with the avian influenza virus. Recognizing that people could be contracted with swine influenza, this study set out to investigate the seroprevalence of individual and multiple infections with two subtypes (H1N1 and H3N2) of the swine influenza virus in pig farms in the Gyeongnam region according to age, area, and season, as well as to provide basic data for the prevention and control of swine influenza. Used in the study were total 904 swine sera that were not vaccinated against the influenza gathered from the pig farms in the Gyeongnam region from November, 2009 to October, 2010. HerdChek SIV (H1N1, H3N2) ELISA kit (IDEXX Laboratories, USA) was used for antibody testing against swine influenza. The test results show that 370 sera (40.9%) were infected with either H1N1 or H3N2 with 37.3% (337 sera) being contracted with H1N1, 13.1% (118 sera) with H3N2, and 9.4% (85) with both H1N1 and H3N2.

• Asian-Australasian Journal of Animal Sciences
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• 제28권11호
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• pp.1573-1582
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• 2015

This study was to investigate the effect of soyabean isoflavones (SIF) on onset of puberty, serum hormone concentration, and gene expression in hypothalamus, pituitary and ovary of female Bama miniature pigs. Fifty five, 35-days old pigs were randomly assigned into 5 treatment groups consisting of 11 pigs per treatment. Results showed that dietary supplementation of varying dosage (0, 250, 500, and 1,250 mg/kg) of SIF induced puberty delay of the pigs with the age of puberty of pigs fed basal diet supplemented with 1,250 mg/kg SIF was significantly higher (p<0.05) compared to control. Supplementation of SIF or estradiol valerate (EV) reduced (p<0.05) serum gonadotrophin releasing hormone and luteinizing hormone concentration, but increased follicle-stimulating hormone concentration in pigs at 4 months of age. The expression of KiSS-1 metastasis-suppressor (KISS1), steroidogenic acute regulatory protein (StAR) and 3-beta-hydroxysteroid dehydrogenase/delta-5-delta-4 isomerase ($3{\beta}-HSD$) was reduced (p<0.01) in SIF-supplemented groups. Expression of gonadotropin-releasing hormone receptor in the pituitary of miniature pigs was reduced (p<0.05) compared to the control when exposed to 250, 1,250 mg/kg SIF and EV. Pigs on 250 mg/kg SIF and EV also showed reduced (p<0.05) expression of cytochrome P450 19A1 compared to the control. Our results indicated that dietary supplementation of SIF induced puberty delay, which may be due to down-regulation of key genes that play vital roles in the synthesis of steroid hormones.

• Journal of Animal Science and Technology
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• 제46권5호
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• pp.729-734
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• 2004

본 연구는 한우의 mtDNA D-loop 영역 염기 변이가 산유량에 미치는 효과를 검증하기 위하여 수행하였다. 젖소의 mtDNA D-loop 영역에서 단일염기의 치환이 20 개의 지역에서 확인되었다. 그중 8, 169, 16042, 16051, 16057, 16093, 16119, 16122, 16209, 16255, 16302 bp 지역에서 높은 빈도의 염기치환이 검출되었다. 16119bp 지역에서는 T가 C로 치환된 빈도가 0 .138로 검출되었으며, 이 위치에서의 염기치환에 의한 산유량 효과는 -1.61 (p<0.1)로 나타났다. 16185bp 지역에서의 염기치환에 의한 산유량 효과는 3.557(p<0.05)로 나타났으며 G가 A로 치환된 빈도는 0.063로 나타났다. 본 연구에서 검출한 한우 다유계통 집단의 mtDNA내 D- loop 영역의 염기서열 변이 빈도와 유량과의 연관성분석 결과 등은 한우 집단의 유전적 변이성 추정과 좀 더 다양한 경제형질과의 관련성 분석으로 다양한 유전적 지표인자 발굴에 도움 될 것이며 이를 통해 분자유전학적 기법을 이용한 한우의 육종 전략을 확립하논데 기초 자료가 되는 것은 물론 우려나라 고유 품종인 한우의 유전자원 보존과 분자육종학적인 연구에 기초 자료로서 유용하게 활용 할 수 있을 것으로 기대된다.

• 한국초지학회:학술대회논문집
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• 한국초지조사료학회 2005년도 학술심포지엄, 제43회 학술발표회
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• pp.192-193
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• 2005