• Title/Summary/Keyword: Live-line

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Ginseng extracts modulate mitochondrial bioenergetics of live cardiomyoblasts: a functional comparison of different extraction solvents

  • Huang, Yun;Kwan, Kenneth Kin Leung;Leung, Ka Wing;Yao, Ping;Wang, Huaiyou;Dong, Tina Tingxia;Tsim, Karl Wah Keung
    • Journal of Ginseng Research
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    • v.43 no.4
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    • pp.517-526
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    • 2019
  • Background: The root of Panax ginseng, a member of Araliaceae family, has been used as herbal medicine and functional food in Asia for thousands of years. According to Traditional Chinese medicine, ginseng is the most widely used "Qi-invigorating" herbs, which provides tonic and preventive effects by resisting oxidative stress, influencing energy metabolism, and improving mitochondrial function. Very few reports have systematically measured cell mitochondrial bioenergetics after ginseng treatment. Methods: Here, H9C2 cell line, a rat cardiomyoblast, was treated with ginseng extracts having extracted using solvents of different polarity, i.e., water, 50% ethanol, and 90% ethanol, and subsequently, the oxygen consumption rate in healthy and tert-butyl hydroperoxideetreated live cultures was determined by Seahorse extracellular flux analyzer. Results: The 90% ethanol extracts of ginseng possessed the strongest antioxidative and tonic activities to mitochondrial respiration and therefore provided the best protective effects to H9C2 cardiomyocytes. By increasing the spare respiratory capacity of stressed H9C2 cells up to three-folds of that of healthy cells, the 90% ethanol extracts of ginseng greatly improved the tolerance of myocardial cells to oxidative damage. Conclusion: These results demonstrated that the low polarity extracts of ginseng could be the best extract, as compared with others, in regulating the oxygen consumption rate of cultured cardiomyocytes during mitochondrial respiration.

The Effects of Live Yeast(Saaccharomyces cerevisiae) Supplementation on the Performance of Laying Hens (활성효모 첨가가 산란계의 생산성에 미치는 영향)

  • 유종석;백인기
    • Korean Journal of Poultry Science
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    • v.17 no.3
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    • pp.179-191
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    • 1990
  • In order to study the effects of supplementation of live yeast(Saccharomyces cerevisiae) on the performance of laying hens, five experiments were conducted. Two experiment were conducted during summer period, one with 37 wk old Dekalb-Delta strain laying birds(Exp. 1) and the other one with 100 wk old molted Nick Chick Brown laying birds(Esp.2) . In each experiment, 240 birds were divided into 12 groups of 20 birds each and randomly distributed. Each of the two experimental diets(Control 71 and 0.05% live yeast supplemented 72) was fed to 6 groups for 4 wks in Exp.1 and 3 wks in Exp.2. Three experiments were conducted during winter period, Exp.2 with 54 wk old Hy-Line strain laying birds, Exp.4 with 52 wk old Hy-Line strain laying birds, and Exp.5 with 36 wk old broiler breeder(Indian River strain). In each experiment, 540 birds were divided into 18 groups of 30 birds each and randomly distributed. Each of the 3 experimental diets(Control:$T_1$0.05% live yeast supplemented:$T_2$ and 0.1% live yeast supplemented : T$_3$) was fed to 9 groups for 6 wks in Exp.3, 9 wks in Exp.4 and 4 wks in Exp.5. In Exp.4, Latin Square design was employed to determine the effects of switching feeds at 3 wk intervals. All hens were housed in cages of commercial farm and experimental diets were made with commercial layer feeds. In Experiment 1, egg production was significantly(P<0.05) higher in $T_2$. Feed intake was significantly (P<higher in 72 at 1st wk but 4 wk average was not significantly different. Feed efficiency was significantly(P<0.01) better in 72 at End wk but 4 wk average was not significantly different. Other parameters, such as weight, soft egg production, cracked egg production and mortality were not significantly different. In Experiment 2, egg production was significantly(P<0.05) higher in $T_2$. Feed efficiency was significantly (P<0.05 and P<0.01) better at End wk and 3rd wk but 3 wk average was not significantly different. Soft egg production was significantly(P<0.05) higher in 72. Other parameters were not significantly different. In Experiment 3, egg productions were significantly(P<0.05) different among treatments : $T_3$ was higher than $T_1$ and $T_2$ was higher than $T_1$. Egg weight of $T_1$ and $T_2$was significantly(P<0.05) heavier than $T_3$. Feed intake of $T_2$ and $T_3$ was significantly(P<0.05) higher than $T_1$ at 6th wk but overall average was not significantly different. Soft egg production were significantly(P<0.01) different among treatments:$T_1$ was higher than $T_3$ was higher than $T_2$. Feed efficiency cracked e99 Production and mortality were not signifcantly different. In Experiment 4, egg production tended to increase as the level of live yeast supplementation increased but they were not statistically different. In Experiment 5, egg production of broiler breeders of $T_3$ was significantly(P<0.01) higher than $T_1$. Feed intake of $T_3$ was significantly(P<0.05) greater than $T_1$ and $T_2$ at 3rd wk but overall average was not significantly different. Fertility and hatchability tended to be higher in the supplemented groups than in the control.

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How Do People Experience Changes and Growths of the Self in On-line Game World? (온라인 게임 세계 속에서 게임 사용자들은 시간이 흐름에 따라 자신의 모습에서 어떠한 변화와 성장을 경험하는가?)

  • Do, Young-Yim;Whang, Sang-Min
    • 한국HCI학회:학술대회논문집
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    • 2009.02a
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    • pp.1208-1217
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    • 2009
  • This research was conducted in an attempt to understand changes and growths of the self in on-line game world. Q methodology was used to investigate players in Mabinogi, which is an on-line game(MMORPG). Based on 57 behavior statements, Players distributed their changing experiences from when they were novices in on-line game world to the current state. In early start phase, they recognized that on-line game world was different from the reality. And they perceived their experiences in on-line game as separated from the real ones. However, as they spend more time in on-line game world, they began to realized that on-line game world is as real as the world because they function as an individual living in a society in both the game and the real world. They felt and learned that in on-line game life, they also need to follow the rules and order. In addition, trust and politeness were essential in order to live with other players. As time passed, the players realized that on-line game experiences have the same meanings and values as the real life ones. 3 types of self-changing patterns were found : Achievement change, Control change, and Relation change. The behavior patterns of the 3 types were then compared to Identify similarities and differences amongst them in psychological meanings and values in the on-line game life. This result illustrates that on-line game world can be defined as new behavioral setting or developmental context established by digital technology. In this new setting, people can experiment and understand their behavior patterns and also experience changes and growths of the self.

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Analysis of Interpretation Processes Through Readers' Thinking Aloud in Science-Related Line Graphs (과학관련 선 그래프를 해석하는 고등학생들의 발성사고 과정 분석)

  • Kim, Tae-Sun;Kim, Beom-Ki
    • Journal of The Korean Association For Science Education
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    • v.25 no.2
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    • pp.122-132
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    • 2005
  • Graphing abilities are critical to understand and convey information in science. And then, to what extent are secondary students in science courses able to understand line graphs? To find clues about the students' interpretation processes of the information in science-related line graphs, this study has the following research question: Is there a difference between the levels of complexity of good and poor readers as they use the thinking aloud method for studying cognitive processes? The present study was designed to provide evidence for the hypothesis that good line graph readers use a specific graph interpretation process when reading and interpreting line graphs. With the aid of the thinking aloud method we gained deeper insight into the interpretation processes of good and poor graph readers while verifying verbal statements with respect to line graphs. The high performing students tend to read much more information and more trend-related information than the low performing students. We support the assumption of differential line graph schema existing in the high performing students in conjunction with general graph schema. Also, high performing students tend to think aloud much more metacognitively than low performing students. High performing students think aloud a larger quantity of information from line graphs than low performing students, and more trend-related sentences than value-related sentences from line graphs. The differences of interpretation processes revealed between good and poor graph readers while reading and interpreting line graphs have implications for instructional practice as well as for test development and validation. Teaching students to read and interpret graphs flexibly and skillfully is a particular challenge to anyone seriously concerned with good education for students who live in an technological society.

Comparison of diseases resistance between white and brown layer lines to experimental infection of Salmonella gallinarum (가금티푸스군의 인공감염에 대한 백색 및 갈색 산란계 계통간의 내병성 비교)

  • Woo, Yong-ku;Kim, Bong-hwan
    • Korean Journal of Veterinary Research
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    • v.38 no.4
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    • pp.784-792
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    • 1998
  • This study was conducted to select resistant chicken-line between Brown and White layer lines against Salmonella gallinarum infection. The Brown and White layer chickens allocated into different age groups were inoculated with S gallinarum (WJO-126) either orally($1{\times}10^7cfu$) or intramusculaly($5{\times}10^6cfu$) and clinical observations were made for 2 weeks. All dead birds were necropsied and culture was made to recover the inoculated organinsm from liver, spleen, brain, bone marrow and cecal contents. Serum was isolated from all live birds after 2 weeks experiment and these birds were also necropsied and cultured to reisolate S gallinarum. The brown layers showed very high mortality to S gallinarum infection regardless of their ages and routes of inoculation, while white layers did not shown any mortality by the direct effects of S gallinarum. The mortality rate of 2 week old brown layers, in particular, were 82.6% (19/23) in peroral group and 86.9% in intramuscularly inoculated group, while those of white layer groups were only 0.0% in both groups. S gallinarum could be reisolated from all dead birds, especially, from liver and spleen. This result was inferred that the organism is highly invasive on the chicken. The intramuscularly challenged birds showed more seropositive-reactors(86.9%) than orally inoculated groups(61.9%). The overall results of present study suggested that white layers are much more resistant than brown layers against the experimental infection of S gallinarum and shown experimentally that resistance to S gallinarum is a characteristic of the White-line layers.

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Comparison of Eye Movement and Fit Rating Criteria in Judging Pants Fit Between Experts and Novices - Using Eye Tracking Technology - (바지 맞음새 평가 시 전문가와 초보자의 시선추적 및 맞음새 평가 항목의 중요도 비교분석 - Eye Tracking 기법을 이용하여 -)

  • Kim, Youngsook;Song, Hwa Kyung;Jang, Hyowoong
    • Fashion & Textile Research Journal
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    • v.19 no.2
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    • pp.230-239
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    • 2017
  • In the clothes industry, there are lack of experts including technical designers who can analyze the fit of clothes. This study is to provide practical data available for fit analysis education by distinguishing the differences in standards and aspects of garment fit between experts and novices, through the eye-tracking technology to quantify the sense of fit. For this study, two groups were organized; one composed of 7 experts with over 15 year-experience including technical designers and patternmakers, and the other composed of 7 novices who are students majoring in clothing. Wearing the goggle type eye-tracker Tobii Pro Glasses 2, the participants in the experiments were required to conduct fit analyses for a pair of pants on a live model. After those experiments, they were required to check the items for fit analysis and assess the importance level of them on a questionnaire. The differences between the two groups in the ratios of total visit count and total visit duration by each AIO(Area of Interest) of clothes were analyzed through non-parametric statistical test. The results of eye tracking experiments showed that experts focused on center front and back line, crotch area, and side seam, while novice's fixation points were dispersed around the pants. The survey results showed that the experts put importance on the center line position and its verticality, front-back proportion of side seam line, and front-back proportion of waist line, 71.4~100% of whom checked them, while 14.3% of the novices checked them.

A Comparative Study on Influence Line of Curved I-Girder Grid Bridge with Constant Cross Section and Variable Cross Section (등·변단면 I-형 곡선격자형교의 영향선에 관한 비교연구)

  • Chang, Byung Soon;Seo, Sang Geun;Ryoo, Eun Yeol;Yun, Jeung Seup
    • Journal of Korean Society of Steel Construction
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    • v.10 no.4 s.37
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    • pp.615-627
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    • 1998
  • In order to determine the maximum shear force, the maximum bending moment, the maximum pure torsion. the maximum warping torsion, and the maximum bimoment for the curved girder grid bridges, it is important to find the location of live load applied to the curved girder grid bridges, so that the influence line can be estimated. The fundamental differential equation concerning the behaviour with warping effects for the curved girder is developed by Vlasov. In this paper, the influence line of shear force, bending moment, pure torsion, warping torsion, and bimoment due to unit vertical load and unit torsional moment for curved I-girder grid bridges with variable and constant cross section are obtained by using the finite difference method and compared with respectively.

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Calculation of Induced Current in the Human Body around 765 kV Transmission Lines (765 kV 초고압 송전선 주변의 인체 유도전류 계산)

  • 명성호;이재복;허창수
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.9 no.6
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    • pp.802-812
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    • 1998
  • Safety related to electric field exposure for the personnel of high voltage power plant and substation is of importance. To analyze the induced current influencing on human body in this paper, we calculate directly capacitance in three dimension which is complex and time consuming, as not to separate the voltage source and the induced object using a effective modeling technique. The proposed algorithm in this paper has been applied to 765 kV high voltage transmission line to evaluate human hazard for the induced current through the case study. As the results, the short circuit current of human body has been identified in the range of 0.3 mA to 6.8 mA. Closing to transmission line, this range of short current can exceed 5 mA that ANSI recommended let-go current. Therefore, it is necessary to countermeasure such as putting on conductive clothing in live-line maintenance of transmission line.

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Slant Estimation and Correction for the Off-Line Handwritten Hangul String Using Hough transform (Hough 변환을 이용한 오프라인 필기 한글 문자열의 기울기 추정 및 교정)

  • 이성환;이동준
    • Korean Journal of Cognitive Science
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    • v.4 no.1
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    • pp.243-260
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    • 1993
  • This paper presents an efficient method for estimationg and correcting the slant of off-line handwritten Hangul strings.In the proposed method,after extracting contours from input image.Hough tranform is applied to the contours to detect lines and estimate slants of the lines.When Hough trans form is applied to the contours,pixels which are not parts of the same stroke could be detected as a line.In order to exclude these lines from slant estimation process,detected lines which have the length less than threshold are eliminated.Experiments have been performed with address images which were extracted from live envelopes provided by Seoul Mail Center.Experimental results show that the proposed method is superior to the previous methods,which had been done with handwritten English strings.in estimation the slant of off-line handwritten Hangul strings.

A Study on Influence Line of Curved I-Girder Grid Bridge with Constant Cross Section (등단면 I-형 곡선 격자형교의 영향선에 관한 연구)

  • Chang, Byung Soon;Ryoo, Eun Yeol;Joo, Jae Hwan
    • Journal of Korean Society of Steel Construction
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    • v.9 no.4 s.33
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    • pp.501-513
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    • 1997
  • The general behavior of curved girder including the warping effects is formulated by series of differential equations postulated by Vlasov. In order to determine the maximum shear force, the maximum bending moment, the maximum pure torsion, the maximum warping torsion, and the maximum bimoment for the curved girder grid bridges, it is important to find the location of live load applied to the curved girder grid bridges, so that the influence line can be estimated. In this paper, the influence line of shear force, bending moment, pure torsion, warping torsion, and bimoment due to unit vertical load and unit torsional moment for curved I-girder grid bridges are obtained by using the finite difference method.

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