• Wind and Structures
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• v.2 no.3
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• pp.151-171
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• 1999

In its 90 years of life, the Tuned Mass Damper have found application in many fields of engineering as a vibration reducing device. The evolution of the theory of TMDs is briefly outlined in the paper. A generalised mathematical linear model for the analysis of the response of line-like structures with TMDs is presented. The system matrices of the system including the TMDs are written in the state space as a function of the mean wind speed. The stability of the system can be analysed and the Power Spectral Density Function of any response parameter calculated, taking into account an arbitrary number of modes of vibration as well as an arbitrary number of TMDs, for any given PSDF of the excitation. The procedure can be used to optimise the number, position and mechanical properties of the damping devices, with respect to any response parameter. Due to the stationarity of the excitation, the method is well suited to structures subjected to the wind action. In particular the procedure allows the calculation of the onset galloping wind speed and the response to buffeting, and a linearisation of the aeroelastic behaviour allows its use also for the evaluation of the response to vortex shedding. Finally three examples illustrate the suggested procedure.

• Bulletin of the Korean Chemical Society
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• v.31 no.2
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• pp.435-441
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• 2010

We report the preparation and characterization of a new and water soluble complex of palladium(II) with 1,10- phenanthroline and butyldithiocarbamate ligands. This compound has been studied through spectroscopic techniques, $^1H$ NMR, IR, electronic spectra and elemental analysis and conductivity measurements. The complex shows 50% cytotoxic concentration ($Ic_{50}$) value against chronic myelogenous leukemia cell line, K562, much lower than that of cisplatin. Thus the mode of binding of this complex to calf thymus DNA have been extensively investigated by isothermal titration UV-visible spectrophotometry, fluorescence, gel filteration and other methods. UV-visible studies show that the complex exhibits cooperative binding with DNA and remarkably denatures the DNA at extremely low concentration ($~13\;{\mu}M$). Fluorescence studies indicate that the complex intercalate into DNA. Gel filtration studies suggest that the binding of Pd(II) complex with DNA is strong enough that it does not readily break. In these interaction studies, several thermodynamic and binding parameters are also determined which may reflect the mechanism of action of this type of compound with DNA.

• Bulletin of the Korean Chemical Society
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• v.32 no.3
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• pp.947-955
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• 2011

The complexes [Pd(bpy)(Hex-dtc)]$NO_3$ and [Pt(bpy)(Hex-dtc)]$NO_3$ (bpy is 2,2'-bipyridine and Hex-dtc is hexyldithiocarbamato ligands) were synthesized and characterized by elemental analysis and spectroscopic studies. The cytotoxicity assay of the complexes has been performed on chronic myelogenous leukemia cell line, K562, at micromolar concentration. Both complexes showed cytotoxic activity far better than that of cisplatin under the same experimental conditions. The binding parameters of the complexes with calf thymus DNA (CT-DNA) was investigated using UV-visible and fluorescence techniques. They show the ability of cooperatively intercalating in CT-DNA. Gel filtration studies demonstrated that platinum complex could cleave the DNA. In the interaction studies between the Pd(II) and Pt(II) complexes with CT-DNA, several binding and thermodynamic parameters have been determined, which may provide deeper insights into the mechanism of action of these types of complexes with nucleic acids.

• Steel and Composite Structures
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• v.22 no.2
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• pp.235-255
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• 2016

This paper experimentally investigated the behavior of steel frame structures of traditional-style buildings subjected to combined constant axial load and reversed lateral cyclic loading conditions. The low cyclic reversed loading test was carried out on a 1/2 model of a traditional-style steel frame. The failure process and failure mode of the structure were observed. The mechanical behaviors of the steel frame, including hysteretic behaviors, order of plastic hinges, load-displacement curve, characteristic loads and corresponding displacements, ductility, energy dissipation capacity, and stiffness degradation were analyzed. Test results showed that the Dou-Gong component (a special construct in traditional-style buildings) in steel frame structures acted as the first seismic line under the action of horizontal loads, the plastic hinges at the beam end developed sufficiently and satisfied the Chinese Seismic Design Principle of "strong columns-weak beams, strong joints-weak members". The pinching phenomenon of hysteretic loops occurred and it changed into Z-shape, indicating shear-slip property. The stiffness degradation of the structure was significant at the early stage of the loading. When failure, the ultimate elastic-plastic interlayer displacement angle was 1/20, which indicated high collapse resistance capacity of the steel frame. Furthermore, the finite element analysis was conducted to simulate the behavior of traditional-style frame structure. Test results agreed well with the results of the finite element analysis.

• YAKHAK HOEJI
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• v.56 no.5
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• pp.299-303
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• 2012

Homosyringaldehyde was isolated and identified from the 80% methanol extract of roots of Cynanchum paniculatum. C. paniculatum has been widely used for the treatment of various diseases such as neurasthenia, insomnia, dysmenorrheal and toothache. This compound exerted significant neuroprotective activities against glutamate-induced neurotoxicity in hippocampal HT22 cell line by 37.53% (at the concentration of $100{\mu}M$). We investigated mode of action of this compound. Homosyringaldehyde ($100{\mu}M$) significantly decreased the ROS level and $Ca^{2+}$ concentration in the oxidative stress induced HT22 cells by oxidative glutamate toxicity. Thus, our results suggest that homosyringaldehyde significantly protect HT22 cells against glutamate-induced oxidative stress, via antioxidative activities. As the results, we suggest that homosyringaldehyde may be useful in the treatment of neurogenerative disorders.

• Wind and Structures
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• v.38 no.4
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• pp.245-259
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• 2024

In a tornadic wind field, the vertical velocity component in certain regions of tornadoes can be significant, forming one of the major differences between tornadic wind fields and synoptic straight-line wind fields. To better understand the wind characteristics of tornadoes and properly estimate the action of tornadoes on civil structures, it is important to ensure that all the attributes of tornadoes are captured. Although Doppler radars have been used to measure tornadic wind fields, they can only directly provide information on quasi-horizontal velocity. Therefore, lots of numerical simulations and experimental tests in previous research ignored the vertical velocity at the boundary. However, the influence of vertical velocity in tornadic wind fields is not evaluated. To address this research gap, this study is to use an approach to derive the vertical velocity component based on the horizontal velocities extracted from the radar-measured data by mass continuity. This approach will be illustrated by using the radar-measured data of Spencer Tornado as an example. The vertical velocity component is included in the initial inflow condition in the CFD simulation to assess the influence of including vertical velocity in the initial inflow condition on the entire tornadic wind field.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7601-7605
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• 2013

Background: Gambogenic acid is a major active compound of gamboge which exudes from the Garcinia hanburyi tree. Gambogenic acid anti-cancer activity in vitro has been reported in several studies, including an A549 nude mouse model. However, the mechanisms of action remain unclear. Methods: We used nude mouse models to detect the effect of gambogenic acid on breast tumors, analyzing expression of apoptosis-related proteins in vivo by Western blotting. Effects on cell proliferation, apoptosis and apoptosis-related proteins in MDA-MB-231 cells were detected by MTT, flow cytometry and Western blotting. Inhibitors of caspase-3,-8,-9 were also used to detect effects on caspase family members. Results: We found that gambogenic acid suppressed breast tumor growth in vivo, in association with increased expression of Fas and cleaved caspase-3,-8,-9 and bax, as well as decrease in the anti-apoptotic protein bcl-2. Gambogenic acid inhibited cell proliferation and induced cell apoptosis in a concentration-dependent manner. Conclusion: Our observations suggested that Gambogenic acid suppressed breast cancer MDA-MB-231 cell growth by mediating apoptosis through death receptor and mitochondrial pathways in vivo and in vitro.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.90-90
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• 2018

Kochiae Fructus is a medicinal plant used as medicine of the urinary organs, skin disease and inflammation. In this study, anti-inflammatory activity of Kochiae Fructus extract and its possible mechanisms of action were examined. The anti-inflammatory activity was investigated by inhibition of lipopolysaccharide (LPS) induced nitric oxide (NO), pro-inflammatory cytokine production and matrix metalloprotease-9 (MMP-9) in murine macrophage-like cell line Raw 264.7 cells. The measurement of the induced pro-inflammatory cytokine levels were carried out by ELISA. The phosphorylation of ERK1/2, JNK and MAPK and the nuclear expression of nuclear factor $NF-{\kappa}B$ p65 were investigated by Wesern blot analysis. The extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of $NF-{\kappa}B$ p65 in activated cells. As a result, we suggest that the extract of Kochiae Fructus decreased the production of pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6), nitric oxide, MMP-9 in LPS-induced Raw264.7 cells. Kochiae Fructus has possibility to be used as therapeutic benefits against inflammatory diseases.

• Journal of Korean Academy of Nursing Administration
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• v.12 no.1
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• pp.41-51
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• 2006

Purpose: The purposed of this study was to develop and evaluate a Web-based for medical terminology educational program. Method: For the development of this program, the NBISD model was applied as a basic model and WBI ID Process Action model was applied. It was executed for 15 weeks along with off-line lectures. After the operation of this program to 210 nursing students, learners' responses were analyzed twice(at 5th week and 15th week). Result: The satisfaction with this program was slightly above the center point. But the structure of system and composition of design were high score. The results of the final evaluation were more positive than those of the interim one. This program have the advantage of easy accessibility and easy understandability with picture and testing. Conclusions: This study proved the importance of the suitability of learning contents and learning form in developing WBI programs and showed possibility for applying WBI to medical terminology. In order to enhance the effects of Web-based medical terminology educational program for, it is necessary to perform analytic comparison with the effect of WBI based on self-directed learning.

• Microbiology and Biotechnology Letters
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• v.47 no.1
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• pp.43-53
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• 2019

The anticancer activity of guava (Psidium guajava L.) leaf extract (GLE) occurs via the induction of apoptosis in cancer cells. However, the mechanism behind GLE-induced apoptosis in the human hepatocellular carcinoma cell line HepG2 remains unclear. In the present study, we investigated the apoptotic effects and mechanism of action of GLE in cultured HepG2 cells. The results showed that GLE induced reactive oxygen species (ROS) synthesis and disrupted the mitochondrial membrane potential (${\Delta}{\Psi}m$). Moreover, GLE increased the expression of apoptotic pathway proteins, such as the cleaved forms of caspase-3, -8, and -9; the translocation of Bax and cytochrome c (cyt-c) from the mitochondria to the cytosol; and the downregulation of Bcl-2. In addition, p53 protein expression was increased upon GLE treatment. These observations indicate that the GLE-induced apoptosis in HepG2 cells is mediated by mitochondrial ROS generation, followed by caspase activation and cyt-c release, suggesting that GLE may be a promising candidate for the development of novel drugs for the treatment of liver cancers.