• Title/Summary/Keyword: Light Curve

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Production of a Monoclonal Antibody to Human $\alpha$-Fetopotein and Development of Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assays for Human $\alpha$-Fetoprotein (인간 $\alpha$-fetoprotein에 대한 모노클로날 항체의 제조 및 모노클로날 항체를 이용한 효소면역분석법의 개발)

  • Michung Yoon;Hyun-Hee Lee;Youngwon Lee
    • Biomedical Science Letters
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    • v.5 no.1
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    • pp.1-10
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    • 1999
  • This study was attempted to generate a monoclonal antibody against human $\alpha$-fetoprotein (AFP) and to produce an immunoassay, recognizing AFP in plasma and amniotic fluid. AFP was purified from human amniotic fluid and used to immunize mice. Spleens were taken from the mice and the cells were fused with mouse myeloma cells (Sp2/0-Ag-14) for the production of monoclonal antibodies by employing the hybridoma technology. As a result, a hybridoma cell line producing anti-AFP monoclonal antibody was cloned out and designated as MabF22. From isotyping analysis, it was found that monoclonal antibody MabF22 was IgG type with IgG1 heavy chain and k light chain. The binding specificity of MabF22 was analyzed by immunoblotting as well as by ELISA. MabF22 was highly specific, reacting with only AFP-containing samples. The binding affinity was determined by ELISA (free-capture mode) and Scatchard analysis. As a result, the value of Kd was 0.8$\times$10$^{-10}$M. The validity of the MabF22 for AFP assay was examined by two kinds of ELISAs, i.e., non-competitive and competitive ELISA. Both assays revealed that MabF22 reacted well with AFP in sample in a concentration-dependent manner. Standard curve and antibody titration curve were obtained by using purified AFP and MabF22. These results indicate that the monoclonal antibody produced in this study would be useful not only for research purposes but also for further development of immune-diagnostic kit for the measurement of AEP concentration.

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Petrochemical Study on the Alkaline Gabbroic Host Rocks of Titaniferous Magnetite Deposits in Gonamsan, Yeoncheon-Gun, South Korea (고남산(古南山) 함(含)티탄자철광상(磁鐵鑛床)의 모암(母岩)인 알카리반려암질암의 암석화학적(岩石化學的) 연구(硏究))

  • Chang, Ho Wan;Yum, Byoung Woo;Park, No Young
    • Economic and Environmental Geology
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    • v.20 no.2
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    • pp.85-95
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    • 1987
  • The host rocks of titaniferous magnetite deposits in Gonamsan are alkaline gabbros, which are typical of undersaturated alkaline rocks in terms of the lack of normative quartz. According to field occurrences and petrographic features, these alkaline gabbros are divided into 3 rock types: coarse-grained and pegmatitic rock, medium-grained rock with equigranular texture, and layered cumulate rock. All these rocks mainly contain clinopyroxene(salite), plagioclase(An 43-66), pargasite, and ilmenite. The accessory minerals are apatite, sphene, quartz, and sometimes titaniferous magnetite. Pargasite, sphene, and quartz are considered to be secondary minerals formed by the reaction among clinopyroxene, plagioclase and Fe-Ti oxide at deuteric stage. Fe-Ti oxides generally occur as ilmenite in the alkaline rocks, and titaniferous magnetite in the ore deposits. Layered cumulate rocks are characterized by a recurrence of discontinuous thin mesolayer of clinopyroxene-pargasite within leucolayer mainly composed of plagioclase. Clinopyroxene is cumulus mineral whereas plagioclase, ilmenite and apatite occur as intercumulus minerals. According to the variation diagrams of oxide and trace element contents against the differentiation index, incompatible elements, such as Na, Ba and Sr, show positive correlations whereas compatible elements, such as Mg and Cr, show negative correlations. However some compatible elements, such as Co, Ni and V show irregular variations, reflecting relative cumulate status of cumulus and intercumulus minerals. On the de la Roche multicationic diagram, these alkaline gabbros are distributed along the differentiation curve of undersaturated alkaline series from alkaline basaltic composition through basanitic composition to tephritic composition. Layered cumulate rocks, which are distributed between basanitic composition and tephritic composition, reflect their cumulate character, slightly scattering away from the curve. The medium-grained rock shows higher contents in Ba, Sr and light rare earch elements than the coarse-grained and pegmatitic rock. The former shows two times higher contents of total rare earth elements than the latter, $while(La/Lu)_{cn}$ ratios maintain fairly constant values of 5.08~5.06 in these two rocks. This means that coarse-grained and pegmatitic rock, as compared with the medium-grained rock, was formed by the earlier differentiated magma but rare-earth element distribution pattern remained almost parallel during differentiation. From the data the above mentioned, these alkaline gabbros are considered to be comagmatic and to be formed by intrusions of differentiated magmas in its reservoir.

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Study of characteristics of $AgGaS_2$/GaAs epilayer by hot wall epitaxy (HWE 방법에 의한 $AgGaS_2$/GaAs epilayer 성장과 특성)

  • Hong, K.J.;Jeong, J.W.;Bang, J.J.;Jin, Y.M.;Kim, S.H.;Yoe, H.S.;Yang, H.J.
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2002.08a
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    • pp.84-91
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    • 2002
  • The stochiometric composition of $AgGaS_2$/GaAs polycrystal source materials for the $AgGaS_2$/GaAs epilayer was prepared from horizontal furnace. From the extrapolation method of X-ray diffraction patterns it was found that the polycrystal $AgGaS_2$/GaAs has tetragonal structure of which lattice constant an and Co were 5.756 $\AA$ and 10.305 $\AA$, respectively. $AgGaS_2$/GaAs epilayer was deposited on throughly etched GaAs(100) substrate from mixed crystal $AgGaS_2$/GaAs by the Hot Wall Epitaxy (HWE) system. The source and substrate temperature were $590^{\circ}C$ and $440^{\circ}C$ respectively. The crystallinity of the grown $AgGaS_2$/GaAs epilayer was investigated by the DCRC (double crystal X-ray diffraction rocking curve). The optical energy gaps were found to be 2.61 eV for $AgGaS_2$/GaAs epilayer at room temperature. The temperature dependence of the photocurrent peak energy is well explained by the Varshni equation, then the constants in the Varshni equation are given by $\alpha=8.695{\times}10^{-4}$ eV/K, and $\beta=332K$. From the photocurrent spectra by illumination of polarized light of the $AgGaS_2$/GaAs epilayer, we have found that crystal field splitting ${\Delta}Cr$ was 0.28 eV at 20 K. From the PL spectra at 20 K, the peaks corresponding to free and bound excitons and a broad emission band due to D-A pairs are identified. The binding energy of the free excitons are determined to be 0.2676 eV and 0.2430 eV and the dissociation energy of the bound excitons to be 0.4695 eV.

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A Study on Growth and Physiological Characteristic for Families in Open-pollinated Progeny Test Forest of Pinus koraiensis in Kapyung Site (가평지역(加平地域) 잣나무 풍매차대검정림(風媒差代儉定林)의 가계별(家系別) 생장(生長)과 생리적(生理的) 특성(特性)에 관한 연구(硏究))

  • Chung, Dong-Jun;Shin, Man Yong;Jang, Yong-Seok;Chon, Sang-Keun
    • Journal of Korean Society of Forest Science
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    • v.90 no.1
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    • pp.124-132
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    • 2001
  • This study was conducted to reveal the relationship between physiological characteristics and growth by family for open-pollinated progeny stand of Korean white pine in Gapyung site. Average growth performance of total 25 families for 18 years old progeny stand in this study site showed 5.9m in mean height, 8.9cm in mean DBH, $12.0m^2$ in basal area per hectare and $46.5m^3$ in volume per hectare, respectively. By the analysis result of growth performance for all 25 families, family No. 20 showed the most excellent growth and middle in family No. 10. On the other hand, family No. 3 represented the worst growth among the 25 families. The change of seasonal photosynthesis rate by increasing luminous intensity was the same tendency as the growth pattern of all 25 families and showed the same results in all four seasons. In the seasonal analysis, the photosynthesis rate was increased as the season has changed from winter to summer. Chlorophyll contents was also increased from winter to summer. Especially, family No. 20 which is the best in growth performance has more chlorophyll contents than the others. In addition, family No. 20 was also better than other families in the weight of assimilation tissue(g), length of needle(cm), width of needle(cm), and number of Stoma. However, in water utilization rate, family No. 3 which is the worst in the growth performance showed the best result that is reverse tendency compared with the photosynthesis rate. The changes of water utilization rate represented to be increased as the season has changed from winter to summer.

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Effects of Multi-layer and TiCl4 Treatment for TiO2 Electrode in Dye-sensitized Solar Cell (염료감응 태양전지의 TiO2 전극의 다중층 및 TiCl4 처리에 따른 효과)

  • Kim, Gyeong-Ok;Kim, Ki-Won;Cho, Kwon-Koo;Ryu, Kwang-Sun
    • Applied Chemistry for Engineering
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    • v.22 no.2
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    • pp.190-195
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    • 2011
  • To investigate the photon-trapping effect and scattering layer effect of $TiO_2$ multi-layer in dye-sensitized solar cell (DSSC) and the degree of recombination of electrons at the electrode treated $TiCl_4$, we formed electrodes of different conditions and obtained the most optimal electrode conditions. To estimate characteristics of the cell, IV curve, UV-Vis spectrophotometer, electrochemical impedance spectroscopy (EIS) and incident photon-to-current conversion efficiency (IPCE) were measured. As a result, we confirmed that the multi-layer's efficiency was higher than that of monolayer in the IV curve and the performance of $TiCl_4$ treated electrode was increased according to decreasing the impedance of EIS. Among several conditions, the efficiency of the cell with scattering layer is higher than that of a layer with the base electrode about 19%. Because the light scattering layer enhances the efficiency of the transmission wavelength and has long electron transfer path. Therefore, the value of the short circuit current increases approximately 10% and IPCE in the maximum peak also increases about 12%.

An Experimental Study on Concrete Bond Behavior According to Grid Spacing of CFRP Grid Reinforcement (격자형 CFRP 보강재의 격자간격에 따른 콘크리트 부착거동에 대한 실험적 연구)

  • Noh, Chi-Hoon;Jang, Nag-Seop;Oh, Hongseob
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.26 no.6
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    • pp.73-81
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    • 2022
  • Recently, as the service life of structures increased, the load-carrying capacity of deteriorated reinforced concrete, where corrosion of reinforcing bars occurs due to various causes, is frequently decreased. In order to address this problem, many studies on the bond characteristic of FRP (Fiber Reinforced Polymer) bars with corrosion resistance, light weight and high tensile strength have been conducted, however there are not many studies on the bond characteristic of grid-typed CFRP embedded in concrete. Therefore, in order to evaluate the bond characteristics of grid-typed CFRP and its usability as a substitute for steel rebar, a pull-out test is performed using the longitudinal bond length and transverse grid length of the grid-typed CFRP as variables. Through the pull-out test, the bond load-slip curve of the grid-typed CFRP is derived, and the bond behavior is analyzed. The total bond load equation is proposed as the sum of the bond force of the longitudinal bond length and the shear force of the grid in the transverse direction. Also, expressing the area of the bond load-slip curve as total work, the change in dissipated energy with respect to the slip is analyzed to examine the effect of the tranverse grid on the bond force.

Image Contrast and Sunlight Readability Enhancement for Small-sized Mobile Display (소형 모바일 디스플레이의 영상 컨트라스트 및 야외시인성 개선 기법)

  • Chung, Jin-Young;Hossen, Monir;Choi, Woo-Young;Kim, Ki-Doo
    • Journal of IKEEE
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    • v.13 no.4
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    • pp.116-124
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    • 2009
  • Recently the CPU performance of modem chipsets or multimedia processors of mobile phone is as high as notebook PC. That is why mobile phone has been emerged as a leading ICON on the convergence of consumer electronics. The various applications of mobile phone such as DMB, digital camera, video telephony and internet full browsing are servicing to consumers. To meet all the demands the image quality has been increasingly important. Mobile phone is a portable device which is widely using in both the indoor and outside environments, so it is needed to be overcome to deteriorate image quality depending on environmental light source. Furthermore touch window is popular on the mobile display panel and it makes contrast loss because of low transmittance of ITO film. This paper presents the image enhancement algorithm to be embedded on image enhancement SoC. In contrast enhancement, we propose Clipped histogram stretching method to make it adaptive with the input images, while S-shape curve and gain/offset method for the static application And CIELCh color space is used to sunlight readability enhancement by controlling the lightness and chroma components which is depended on the sensing value of light sensor. Finally the performance of proposed algorithm is evaluated by using histogram, RGB pixel distribution, entropy and dynamic range of resultant images. We expect that the proposed algorithm is suitable for image enhancement of embedded SoC system which is applicable for the small-sized mobile display.

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Degradation Mechanisms of Organic Light-emitting Devices with a Glass Cap (유리 덮개로 보호된 OLED소자의 발광특성 저하 연구)

  • Yang Yong Suk;Chu Hye Yong;Lee Jeong-Ik;Park Sang-He;Hwang Chi Sun;Chung Sung Mook;Do Lee-Mi;Kim Gi Heon
    • Journal of the Korean Vacuum Society
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    • v.15 no.1
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    • pp.64-72
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    • 2006
  • We demonstrated organic light-emitting devices (OLEDs) based on the organic thin-film materials such as tris-(8-hydroxyquinoline) aluminum $(Alq_3)$. The structure of OLEDs was vacuum deposited upon transparent and thin glass substrates pre-coated with a transparent, conducting indium tin oxide thin film. The luminance characteristics, current, capacitance, and dispersion factor for degraded OLEDs, which were made by various bias currents $(0.5mA\;{\leq}\;I_{Bias}\;{\leq}9mA)$, are studied. The current dependences of lifetime were divided at approximately 2mA, and they represented nearly linear behaviors but had different slopes in a logarithmic plot of lifetime versus bias current. With lighting OLEDs, the anomaly of capacitance, as shown in the CV curve, occurred because of two factors, polarization in the bulk of organic materials and the interface between the metal and organic layers. In decayed OLEDs that had lower bias currents of less than 2mA, it was found that the degradation of luminance was related to both the decrease of polarization and to the lowering of the injection barrier.

Image Quality Evaluation of Medical Image Enhancement Parameters in the Digital Radiography System (디지털 방사선시스템에서 영상증강 파라미터의 영상특성 평가)

  • Kim, Chang-Soo;Kang, Se-Sik;Ko, Seong-Jin
    • The Journal of the Korea Contents Association
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    • v.10 no.6
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    • pp.329-335
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    • 2010
  • Digital imaging detectors can use a variety of detection materials to convert X-ray radiation either to light or directly to electron charge. Many detectors such as amorphous silicon flat panels, CCDs, and CMOS photodiode arrays incorporate a scintillator screen to convert x-ray to light. The digital radiography systems based on semiconductor detectors, commonly referred to as flat panel detectors, are gaining popularity in the clinical & hospital. The X-ray detectors are described between a-Silicon based indirect type and a-Selenium based direct type. The DRS of detectors is used to convert the x-ray to electron hole pairs. Image processing is described by specific image features: Latitude compression, Contrast enhancement, Edge enhancement, Look up table, Noise suppression. The image features are tuned independently. The final enhancement result is a combination of all image features. The parameters are altered by using specific image features in the different several hospitals. The image in a radiological report consists of two image evaluation processes: Clinical image parameters and MTF is a descriptor of the spatial resolution of a digital imaging system. We used the edge test phantom and exposure procedure described in the IEC 61267 to obtain an edge spread function from which the MTF is calculated. We can compare image in the processing parameters to change between original and processed image data. The angle of the edge with respect to the axes of detector was varied in order to determine the MTF as a function of direction. Each MTF is integrated within the spatial resolution interval of 1.35-11.70 cycles/mm at the 50% MTF point. Each image enhancement parameters consists of edge, frequency, contrast, LUT, noise, sensitometry curve, threshold level, windows. The digital device is also shown to have good uniformity of MTF and image parameters across its modality. The measurements reported here represent a comprehensive evaluation of digital radiography system designed for use in the DRS. The results indicate that the parameter enables very good image quality in the digital radiography. Of course, the quality of image from a parameter is determined by other digital devices in addition to the proper clinical image.

Development of OSL Dosimetry Reader (선량 판독용 OSL 측정장치의 개발)

  • Park, Chang-Young;Chung, Ki-Soo;Lee, Jong-Duk;Chang, In-Su;Lee, Jung-Il;Kim, Jang-Lyul
    • Journal of Radiation Protection and Research
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    • v.37 no.1
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    • pp.10-15
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    • 2012
  • Design and performance test results of a newly developed optically stimulated luminescence (OSL) measurement system are presented in this paper. Generally, different types of optical filters are used in OSL reader system to minimize the interference of the stimulation light in the OSL signal. For optically stimulation of $Al_2O_3:C$, we have arrived at an optimal combination of the filters, i.e., GG420 filter for filtering the stimulating light source, and a combined UG11 and BG39 filter at the detecting window (PMT). By using a high luminance blue LED (Luxeon V), sufficient luminous intensity could be obtained for optically stimulation. By using various control boards, the OSL reader device was successfully interfaced with a personal computer. A software was developed to deliver required commands to operate the OSL reader by using the LabView program (National Instruments, Inc.). In order to evaluate the reliability and the reproducibility of newly designed-OSL reader. Performance testing of the OSL reader was carried out for OSL efficiency, OSL decay curve and signal to noise ratio of the standard $Al_2O_3:C$ OSL material. It was found to be comparable with that of commercial Riso reader system.