• 사회복지연구
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• 제40권2호
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• pp.313-335
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• 2009

본 연구는 자원봉사활동 참여가 청소년의 자아존중감 변화에 미치는 영향을 분석하고자 하였다. 이를 위해 한국청소년정책연구원의 청소년패널자료 중 중학교 2학년 패널 3개년(2005-2007년) 자료가 활용되었고, 분석방법은 변수 간 종단적인 변화의 관계 구조를 추정하는데 적합한 분석 도구인 고정효과모형(Fixed Effects Modeling)을 이용하여 분석하였다. 연구결과 자원봉사활동 참여는 청소년의 자아존중감의 증가를 가져오는 것으로 나타났다. 또한 개인요인, 가족요인, 학교요인 중 생활만족수준이 높을수록, 외모스트레스가 낮을수록, 부모 및 형제자매와의 관계의 만족수준이 높을수록, 성적이 높을수록, 교사와의 관계의 만족수준이 높을수록, 친구와의 관계의 만족수준이 높을수록 자아존중감 수준이 높아지는 것으로 나타났다. 본 연구는 기존의 횡단적 연구의 한계를 보완하여 자원봉사활동 참여와 자아존중감의 관계를 보다 분명히 검증하였다는 의의를 갖는다. 또한 청소년의 자아존중감 향상을 위해서 가족과 학교, 지역사회를 통하여 자원봉사활동의 활성화를 위한 포괄적인 실천적, 정책적 지원이 필요함을 시사하고 있다.

• 대한물리치료과학회지
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• 제30권4호
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• pp.71-81
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• 2023

Background: The purpose of this study was to comparison and correlation between physical and psychological functions by gender in Judo athletes. Both the physical and psychological factors of athletes are important as factors influencing athletic performance, but there are few papers that have compared and studied the correlation between physical and psychological functions of judo players. Design: Cross sectional study Methods: The 134 participants; high school students, college students, and team players. They used physical functions to measure such as self-describing KOOS, FAOS, ODI, POMS, SCAT, ASMQ, and ACSI-28. Results: There was a significant difference in the physical function according to gender in the KOOS, but no significant difference in the FAOS and ODI. For psychological function, there was a significant difference in the vitality item in the POMS, and a significant difference in the body management, training management, life management, and unique behavior management items in the ASMQ. In the ACSI-28, there were significant differences in coping with adversity, stress repayment, concentration, freedom to worry about matches, and self-coaching behavior items. Physical function and psychological function showed a significant negative correlation. The POMS showed a negative correlation between the ASMQ and ACSI-28, whereas the SCAT showed a positive correlation. Conclusion: The results of this study are expected to be used as a basic data for sports training by gender of Judo athletes.

• Mycobiology
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• 제51권6호
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• pp.379-387
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• 2023

In this study, twenty-five yeast strains were isolated from soil samples collected in the gold mining ore in Gia Lai, Vietnam. Among them, one isolate named GL1^T could highly tolerate Cu²⁺ up to 10 mM, and the isolates could also grow in a wide range of pH (3-7), and temperature (10-40 ℃). Dried biomass of GL1 was able to remove Cu²⁺ effectively up to 90.49% with a maximal biosorption capacity of 18.1 mg/g at pH 6, temperature 30 ℃, and incubation time 60 min. Sequence analysis of rDNA indicated this strain was closely related to Rhodotorula mucilaginosa but with 1.53 and 3.46% nucleotide differences in the D1/D2 domain of the 28S rRNA gene and the ITS1-5.8S rRNA gene-ITS2 region sequence, respectively. Based on phylogenetic tree analysis and the biochemical characteristics, the strain appears to be a novel Rhodotorula species, and the name Rhodotorula aurum sp. nov. is proposed. This study provides us with more information about heavy metal-tolerant yeasts and it may produce a new tool for environmental control and metal recovery operations.

• 대한불안의학회지
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• 제19권2호
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• pp.61-68
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• 2023

Objective : The purpose of this study was to investigate the associations between psychosocial factors and the mask-wearing behavior after deregulation of COVID-19 quarantine guidelines among adults in Korea. Methods : We collected data (345 subjects) from online questionnaire survey. The questionnaire included the Korean version of the Patient Health Questionnaire-9, the Korean version of fear of COVID-19 Scale, the Korean version of the Patient Health Questionnaire-15, Korean versions of the Perceived Stress Scale, and measurement tools adapted from previous studies for COVID-19 risk perception, social stigma, and appearance interest of subjects. We analysed data using SPSS version 23.0 for descriptive statistics, chi-square test, and logistic regression analysis. Results : People with cohabitant or mask-wearing tendency before COVID-19 pandemic had a higher rate of mask-wearing than those who were not. Subjects reporting higher level of social stigma (OR=1.154, 95% CI 1.049-1.270) and COVID-19 anxiety (OR=1.072, 95% CI 1.007-1.141) were more likely to report maskwearing behavior. Conclusion : From the results, appropriate intervention to those who fear social stigma and are anxious to the infectious diseases will be needed. Additionally, providing policies and guidelines that consider cohabitants and offering continuous education with information of disease to the public are also expected to helpful for recovery of daily life from infectious diseases.

• 대한통합의학회지
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• 제12권1호
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• pp.11-26
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• 2024

Purpose : This study investigated the empowerment, burnout, and job satisfaction levels among occupational therapists, and to ascertained the effects of empowerment and burnout on job satisfaction. The study aimed to provide foundational data to help increase job satisfaction among occupational therapists. Methods : We surveyed 180 occupational therapists working in the Jeollabuk-do. The survey included 49 questions covering general characteristics, empowerment, burnout, and job satisfaction. Differences in empowerment, burnout, and job satisfaction levels based on general characteristics were analyzed using independent sample t-tests and one-way ANOVA. We performed multiple regression analyses to ascertain the effects of empowerment and burnout on job satisfaction. Results : The average scores for job satisfaction, burnout, and empowerment were 40.32, 58.40, 41.82, respectively. Job satisfaction according to general characteristics showed statistically significant differences based on the annual income and the most difficult treatment. We found that empowerment factors such as perceived importance of the job confidence in one's abilities, and independence and autonomy in job performance, significantly affected job satisfaction. However, the most significant factor influencing job satisfaction was daily fatigue and stress. Conclusion : Job empowerment and burnout of significantly affected job satisfaction among occupational therapists. Occupational therapists have a effect on the daily lives of their clients through interventions, playing a crucial role for each individual client. Thus, increasing the empowerment and job satisfaction levels of occupational therapists and reducing their burnout levels can help improve the quality of life of the clients and enable occupational therapists to provide high-quality services.

• 동의신경정신과학회지
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• 제35권1호
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• pp.15-26
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• 2024

Objectives: The objective of this study is to develop a specialized clinical research protocol for acupuncture treatment specifically designed for disaster survivors based on insights from an expert survey. Methods: An expert panel comprising specialists in neuropsychiatry, acupuncture, and clinical research methodology was assembled. Initial data to inform the clinical research protocol design was collected utilizing open-ended responses, multiple-choice questions, and a 5-point Likert scale to gauge agreement levels. Next, this data was disseminated to a panel of experts. A cohesive clinical research protocol was then formulated during a core panel meeting by integrating insights from a panel of 10 experts. Results: The protocol developed herein entails a non-randomized controlled study involving participants aged 19~64 years old who have been identified as high-risk or cautious according to the National Trauma Center screening test. The study design includes the establishment of an active control group, which allows for the assessment of an additional effect through comparison with conventional therapy. The selected acupuncture approach involves a combination of manual acupuncture and ear acupuncture. For clinical outcome assessment, the Clinician-Administered Posttraumatic Stress Disorder Scale for DSM-5 was proposed to gauge trauma symptoms. Representative scales for various domains such as depression, anxiety, anger, insomnia, pain, and quality of life were also provided for reference. Conclusions: The developed protocol is anticipated to streamline the swift design and initiation of clinical trials during disaster scenarios. It is also designed to be scalable, thereby enabling its application in both non-randomized control group studies and single-group before-and-after comparisons.

• FOCUS: LIFE SCIENCE
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• 제1호
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• pp.2.1-2.6
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• 2024

This article provides detailed instructions for the correct installation, maintenance, and troubleshooting of capillary gas chromatography (GC) columns. It emphasizes the importance of proper installation to ensure optimal performance and longevity of the column. The document covers various aspects such as column trimming, installation, conditioning, testing, storage, and ferrule selection. The installation process involves ensuring that the heated zones of the GC are cool before placing the column cage in the column oven. It is essential to avoid sharp bends or stress on the capillary column during installation and to connect the front end of the column into the GC inlet at the recommended insertion distance. The document also provides guidance on trimming the column, including the use of a ceramic wafer or capillary column cutter to achieve a clean, burr-free cut. For previously used columns, it recommends removing any capillary caps, positioning the nut and ferrule, and trimming 1-2 cm from the column. After installation, the column should be purged with carrier gas to remove any oxygen and avoid oxidizing the column. Conditioning the column involves ramping to the upper isothermal temperature limit and maintaining this temperature for a specified duration. It is crucial to maintain carrier gas flow during conditioning and not exceed the upper temperature limit of the column to avoid phase damage. The document also discusses testing column performance using a suitable method and performing a test injection to assess performance. It provides recommendations for column storage, including flame-sealing the capillary ends or using retention gaps for long-term storage. Additionally, it emphasizes the importance of routine maintenance and replacement of GC consumables to extend the column's lifetime. Ferrule selection is another important aspect covered in the article, with a variety of ferrule materials available for different applications. The characteristics of common ferrule options are presented in a table, including temperature limits, reusability, and suitability for specific detector types.

• 한국구조물진단유지관리공학회 논문집
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• 제12권1호
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• pp.101-108
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• 2008

본 연구에서는 철근과 같은 기계적 맞물림 현상을 활용하기 위하여 이형리브가 형성되어 있는 GFRP 보강근을 제작하여 철근대체 재료로 사용하기 위해 FRP 보강근의 부착성능을 규명하고자 한다. 하지만 지금까지 많은 기존 연구자들이 부착성능에 대한 실험으로 단순 1방향(수직, 수평)인장실험으로 철근과 콘크리트 또는 FRP 보강근과 콘크리트사이의 부착특성을 고찰하여 두 재료 사이의 부착-슬립에 관한 제안식을 도출해왔다. 국내에서는 아직까지 GFRP 보강근의 부착에 대한 관심이 증대대고 있는 실정이지만 피로부착에 관한연구는 미흡한 편이이어서 GFRP 보강근의 피로 연구가 필요로 하다. 본 연구에서는 BRITISH STANDARD에서 규정하고 있는 방법에 의하여 휨 부착 시험체를 제작하여 정적 휨 부착실험 최대파괴하중의 70% ~ 90%의 하중으로 반복하중재하 후 정적실험을 통하여 GFRP로 보강된 콘크리트 피로부착 성능을 검증하였다.

• 생명과학회지
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• 제19권5호
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• pp.633-638
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• 2009

저온 진공 공정으로 건조된 참치를 유기용매로 추출하여 이들 참치 추출물 및 분획물들에 의한 인체 결장암 및 섬유육종세포에 대한 증식 및 세포 내 활성산소종 억제 효과에 대해 검토하였다. 건조 참치의 A+M 추출물과 MeOH 추출물을 0.5, 1, 및 5 mg/ml의 농도로 인체 섬유육종세포(HT1080)에 처리했을 때 농도 의존적으로 인체 암세포 증식을 억제시켰으며(p<0.05), 특히 A+M 추출물이 MeOH 추출물에 비하여 그 억제효과가 우수하였다. 건조 참치 추출물로부터 얻어진 분획물들의 경우 특히 hexane과 85% aq. MeOH 분획물들에 의한 암세포 증식 억제효과가 높았다. 인체 결장암세포(HT-29)에 대한 결과로 A+M 추출물은 인체 섬유육종세포(HT1080)의 결과와 비교했을 때 다소 암세포 증식 억제 효과가 낮았으나 5 mg/ml의 첨가농도에서 95%로 암세포 증식 억제효과를 보였고(p<0.05), MeOH 추출물도 앞서 A+M 추출물 결과와 유사하게 인체 섬유육종세포(HT1080)에 비해 낮은 암세포 성장 억제효과를 보였지만, 첨가농도 5 mg/ml에서는 93%로 높은 암세포 증식 억제효과를 나타내었다(p<0.05). 건조 참치 분획물들의 경우도 앞서의 인체 섬유육종세포(HT1080)와 유사하게 모든 분획물들에서 농도 의존적으로 억제효과가 높은 것을 살펴 볼 수가 있었고, 인체 결장암세포(HT-29)에서도 hexane과 85% aq. MeOH 분획물에 의한 항암효과가 우수하였다. In vitro 항산화실험에서 건조 참치 A+M 추출물 및 MeOH 추출물을 농도별로 인체 섬유육종세포(HT1080)에 처리하였을 때 A+M 추출물(10 mg/ml 농도)를 제외하고 측정기간 120분 동안 모든 추출물들이 blank군과 control군에 비해 세포 내 활성산소종을 크게 감소시켰다. 건조 참치 분획물들 중 BuOH 및 85% aq. MeOH 분획물들은 세포내 활성산소종을 크게 감소시키는 우수한 항산화 효과를 나타내었다.

• Journal of Plant Biotechnology
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• 제41권2호
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• pp.81-88
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• 2014

식물생명공학 연구에 있어서 모델 식물인 Arabidopsis thaliana (애기장대)와 아주 유사한 염생 식물인 salt cress (Thellungiella halophila 또는 Thellungiella parvula)는 고염 스트레스에 대하여 강한 내성을 가지고 있다. 본 연구에서는 고염에 저항성을 가지는 유용유전자를 선별하기 위하여, 200 mM NaCl을 처리한 T. halophila 또는 T. parvula 식물로부터 mRNA를 분리하여 cDNA library를 작성하였다. Full length cDNA library를 Agrobacteria-methods 형질전환 방법에 필요한 binary vector pool을 작성하고 Arabidopsis에 형질전환 시켰다. 형질전환되어진 Arabidopsis를 항생제 Basta 선별을 통하여 형질전환체 pool을 구축하였다(305,400 종자). 이와 같은 방법을 통해 구축 되어진 pool중에서 168,500 종자를 이용하여 고염 스트레스 조건하에서 종자 발아율과 뿌리 생장 촉진되는 현상이 나타나는 내염성 형질전환체를 1차 선별하였다(7,157 개체; 4.24%). 1차 선별된 형질전환체 중에서 1,551개체를 이용하여 2차 선별을 수행하여 내염성 형질전환체 165 개체를 확보하였다(10.6%). 선별된 형질전환체 중 대부분 개체는 고염 스트레스에 대응하여 종자 발아 및 뿌리 생장 모두 야생형보다 촉진된 표현형을 보였다. 그 중 몇 몇의 형질전환체는 야생형에 비하여 특이적인 기관 발달 현상이 나타났다. 예를 들면, 꽃과 줄기의 발달이 야생형과는 다른 표현형을 보이는 형질전환체가 선별되었다. 이렇게 스트레스에 내성을 가지는 형질전환체로부터 유전자 분리 방법을 통하여 해당 유용유전자를 확보할 수가 있다. 본 연구에서는 향후 halopyte 식물체를 이용하여 고염 뿐만 아니라 다양한 환경스트레스(건조, 냉해, 고열, 호르몬 등) 신호전달에 관여하는 유용유전자를 보다 쉽게 확보하는 방법을 제시함으로서, 환경재해극복에 관여하는 신호전달 기작을 보다 쉽게 이해할 수 있을 것으로 사료된다.