• Proceedings of the Korean Society of Crop Science Conference
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• 1999.05a
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• pp.162-163
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• 1999

The goal of this research was (1) to describe the conditions and parameters required for the cell cycle synchronization and the accumulation of large number of metaphase cells in maize and other cereal root tips, (2) to isolate intact metaphase chromosomes from root tips suitable for characterization by flow cytometry, and (3) to construct chromosome-specific libraries from maize. Plant metaphase chromosomes have been successfully synchronized and isolated from many cereal root-tips. DNA synthesis inhibitor (hydroxyurea) was used to synchronize cell cycle, follwed by treatement with trifluralin to accumulate metaphase chromosomes. Maize flow karyotypes show substantial variation among inbred lines. thish variation should be sueful in isolating individual chromosome types. In addition, flow cytometry is a useful method to measure DNA content of individual chromosomes in a genotyps, and to detect chromosomal variations. Individual chromosome peaks have been sorted from the maize hybrid B73/Mol7. Libraries were generated form the DOP-PCR amplification product from each peak. To date, we have analyzed clones from a library constructed from the maize chromosome 1 peak. Hybridization of labeled genomic DNA to clone inserts indicated that $24\%,\;18\%,\;and\;58\%$ of the clones were highly repetitive, medium repetitive, and low copy, respectively. Fifty percent of putative low cpoy clones showed single bands on inbred screening, blots, and the remaining $50\%$ were low copy repeats. Single copy clones showing polymorphism will be mapped using recombinant inbred mapping populations. Repetitive clones are being characterized by Southern blot analysis, and will be screened by in situ hybridization for their potential utility as chromosome specific markers.

• Korean journal of applied entomology
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• v.54 no.3
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• pp.247-255
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• 2015

The cotton aphid, Aphis gossypii Glover (Hemiptera: Aphididae) infests citrus orchards, causing sooty mold damage to the fruits. This study was conducted to investigate the colonizing route of A. gossypii in citrus orchards in a non-heating plastic-film house during the early season. The overwintering eggs of the aphids are frequently found on summer shoots of the citrus trees. The eggs were mostly those of Aphis citricola, without any A. gossypii when hatched. The colonization of citrus trees by alate A. gossypii in non-heating plastic-film houses was mainly observed twice, with advanced flight in late April and delayed flight in late May. The delayed flight was synchronized with the timing of the emergence of alate A. gossypii from the fundatrix generation in the holocyclic life cycle. During advanced flight in closed citrus orchards, alate A. gossypii were caught in yellow water traps installed in the fields, and the populations were found to originate from the surviving populations of the anholocyclic life cycle. Consequently, we concluded that citrus tree colonization with A. gossypii occurred during the advanced flight of the anholocyclic and the delayed flight of the holocyclic life cycle.

• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1453-1458
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• 2008

For quantitative PCR assay of Salmonella enterica serovar Typhimurium in food samples, a real-time PCR method was developed, based on DNA genome equivalent. Specific primers and probe designed based on the STM4497 gene of S. Typhimurium LT2 showed the specificity to S. Typhimurium. Threshold cycle (Ct) values of real-time PCR were obtained from a quantitative standard curve with genomic DNA of Salmonella Typhimurium. In addition, the recovery of S. Typhimurium inoculated artificially to chicken samples with $4.5{\times}10^5$ to 4.5 CFU/ml was evaluated by using real-time PCR and plate-count methods. Result showed that the number of cells calculated from the real-time PCR method had good correlation with that of the plate-count method. This real-time PCR method could be applicable to the detection and quantification of S. Typhimurium in food samples.

• Korean Management Science Review
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• v.27 no.1
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• pp.107-116
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• 2010

There are several stage in corporate's life cycle such as foundation, growth, maturity or bankruptcy. A bankruptcy is very important for corporate in the life cycle. Especially, venture business' life cycle is short compare to other type of corporate. A lot of venture businesses have emerged and bankrupted soon in the market. Venture businesses' survival or bankruptcy have been influenced by not only external environment like the rate of exchange, oil price, and foreign exchange crisis but also internal environment such as efficiency, process, human resources, finance and CEO. In this paper, we attempt to examine financial factors and efficiency that influence on the venture businesses' survival and bankruptcy. The more venture businesses have high efficiency score, the more they have high probability of survival.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.24 no.68
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• pp.51-65
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• 2001

This study is to make LCIA(Life Cycle Impact Assessment) easier as a methodology of environmental scores(called E-score) that integrated environmental load of each emission substance based on environmental damage such as in human health, ecosystem and resources category. The concept is to analyzes the LCI(Life Cycle Inventory) and defines the level of environment damages for human health, ecosystem and resources to objective impact assessment standard, and makes the base of marginal damage to calculate the damage factor, which can present the indication that can establish the standard value of environmental impact. First, damages to human health are calculated by fate analysis, effect analysis and damage analysis to get the damage factor of health effect as a DALY(Disability Adjusted Life Years) unit. Second, damages to ecosystem are calculated by fate analysis, effect analysis and damage analysis to get the damage factor of the effect as a PDF(Potentially Disappeared Fraction) unit through linking potentially increased disappeared fraction. Third, damages to resources are carried out by resource analysis and damage analysis for linking the lower fate to surplus energy conception to get damage factor as a MJ(Mega Joule) unit. For the ranking of relative environment load level each other, LCIA can be carried out effectively by applying this E-score methodology to the particular emission substances. A case study has been introduced for the emission substances coming out of a tire manufacturer in Korea. It is to show how to work the methodology. Based on such study result, product-designers or producers now can apply the E-scores presented in this study to the substances of emission list, and then calculate the environment load of the product or process in advance at any time and can see the environment performance comparatively and expected to contribute to the environmental improvement in view of environmental pollution prevention.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.2
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• pp.297-305
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• 2016

This study aims to analyze characteristics of environmental load for the construction phase of PSC beam bridge based on Life Cycle Assessment. For detail computation of environmental load, the construction materials and energy consumption are derived from the BOQ, also connecting with environmental load by Korea LCI Database Information Network. The characteristic of environmental impact was analyzed by 25 cases and cut-off ratio was 80% to 94%. The result sorted by construction materials revealed that environmental load were 53.3% for ready-mixed concrete, 9.6% for wire rod, 7.8% for rebar, 6.8% for cement, 5.5% for plywood, and 5.2% for energy. Furthermore, the result of environmental impact revealed that 45.5% for global warming, 30.4% for abiotic resources depletion, 10.5% for human toxicity, and 8.9% for photochemical oxidant creation. In the future, we can make a decision considering environmental load based on LCA at design phase.

• Journal of the Korean Institute of Educational Facilities
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• v.20 no.3
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• pp.53-61
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• 2013

The purpose of this study is to suggest the way how to improve the school green building certification system based on life cycle assessment methodology and to assess $LCCO_2$ in outline. Green Building Certification System for School is comprised of 7 categories and 39 items. 7 categories include Land use and Transport, Energy and Atmosphere, Materials and Resources, Water, Management, Ecology, Indoor environmental quality. School is a public facility for students. So Green Building Certification System for School must have educational point of view adding to energy saving, reduction of greenhouse gas emission, etc. Also it needs to be classified into three categories ; kindergarten, elementar/middl/high school and university. Improvement plans for items are as follows ; energy consumption and $LCCO_2$ assessment considering life cycle, deletion or integration of duplicate items by comparing other systems, application of passive solar systems, consideration of the law and standards change, and selection of items considering specific building use.

• The Journal of Korean Academic Society of Nursing Education
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• v.18 no.3
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• pp.436-445
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• 2012

Purpose: The purpose of this study was to identify social support networks for each life-cycle stage of adults. Method: A total of 1,047 subjects included 454 young adults, 262 middle-aged adults and 331 senior adults. Data were collected using Oh's Korean Version Norbeck's Social Support Questionnaire (NSSQ), and analyzed using descriptive statistics, t-test, and ANOVA. Result: For the young adults, parents were the top and second priority as important social support resources, the third was siblings, and then friends. For the middle-aged, spouse was the first priority as an important social support resource, while the second and the third were children. For the senior adults, children ranked from the top to the seventh priority. The mean number of social support resources was 13.23 for the young adult, 12.93 for the middle-aged and 5.30 for the senior adults. Social support networks of the young adults significantly differed according to gender and marital status. That of the middle-aged significantly differed according to family size. In addition, that of the senior adults was significantly different according to marital status, economic status, religion and family size. Conclusion: It is essential to consider social support networks for each life-cycle stage of adults when making a social support intervention program.

• Resources Recycling
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• v.11 no.4
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• pp.17-26
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• 2002

As it is not allowed to landfill sludge from 2001 by the act of waste management, new systems of treating sludge are necessary. Life Cycle Assessment, LCA, is a method for evaluating systems in the aspect of environment and also can apply to decision making tools for policy making. The objective of this study is to assess 3 alternatives of landfill: incineration, composting, solidification by applying LCA. This study is done with operation data from incinerator in Kuri, composting facility in Nanjido, solidification facility in Kimpo and electricity and transport data of Korea in 1998 are used. The results of the LCA is that the composting system is most environmental-friendly and the solidification system is least environmental-friendly.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.6
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• pp.25-40
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• 2015

Korean tourist areas are designated/managed in accordance with the Tourism Promotion Act. Since Taejongdae was designated as a tourist area in 1969, a total of 230 tourist areas have been designated/built/operated. It has been 46 years since the first tourist area was designated. According to the Tourism Area Life Cycle by Butler, it is estimated that the flow of the life cycle will be reflected in ups and downs over time. Thus, this study aimed to provide basic data for suggesting the direction of changes and development of the future tourist areas after analyzing the life cycle stage of domestic tourist areas, by applying the Tourism Area Life Cycle by Butler. The research method was based on the tourist areas by year, the number of visitors, and data of the target to derive the change transition curve, obtained by dividing the life cycle stages of the tourist areas based on the visitor rate of change. In the analysis results, more than 1/3 of domestic tourist areas are reaching the stagnation/decline stage, and tourist areas such as hot springs and seaside/beach resources show a particularly high ratio of stagnation/decline. The tourist areas that already have reached the stagnation/decline stage will need to analyze the causes for the decline, seek for resolution measures, and introduce new innovative elements. Even though the results of this study are not sufficient to be used as an absolute standard to decide the life cycle stage of domestic tourist areas, it is considered to be adequate for phenomenologically understanding the life cycle stage of Korean tourist areas. Based on this study, the causes for the stagnation/decline of tourist areas can be revealed while it can be also used as basic research to establish revitalization measures for tourist areas by introducing new innovation.