• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.38 no.4
• /
• pp.305-310
• /
• 2012

Through our entire life, oral care products such as toothpaste are used. Thus the safety of oral care products used every day to our mouth is very important. As the previous study in animal tests or clinical trials, surfactant in toothpaste may cause the oral irritation. However, EU cosmetics legislation prohibits animal testing of cosmetics and its ingredient for animal welfare. Therefore the development of alternative in vitro test has been actively performed to replace or reduce using the animal in many areas. However, the way to evaluate oral mucosal toxicity has been done using animal models or clinical trials from now on. Even more, the experiment with human oral 3D tissue or human oral cell line is used recently. The aim of this study is the development of oral mucosal irritation method without using animal for the safety of the oral care product. We developed in vitro test method for oral irritation by using human oral cell line (YD-38 cell) acceptable to toothpaste which contains insoluble material. By the results of this assay, we could discriminate toothpaste with or without irritating substance as same manner in animal studies reported previously. In addition, we confirmed that toothpaste for babies and children toothpaste irritated oral musoca lower than the general adult toothpaste. The present study suggest that this new in vitro method by using human oral cell line (YD-38 cell) could be used for evaluation of oral irritation without using animal.

• Journal of Fisheries and Marine Sciences Education
• /
• v.20 no.1
• /
• pp.127-134
• /
• 2008

The purpose of this study was to determine content of biogenic amines (Histamine, Cadaverine, Putrescine) in the salted mackerel, canned mackerel, mackerel pike, and freeze dried mackerel pike (Guamegi) collectively, all were purchased in the market. Salted mackerel was purchased at five markets (A, B, C, D, and F market) and stored for 10 days at $4{^{\circ}C}$. At the time of purchase, salted mackerel purchased at markets A, B and C was tested for biogenic amine, yet nothing was detected. However, salt mackerel purchased at market D was tested and the histamine concentration was 0.5 mg/100g. Mackerel from market F, exhibited content levels of 0.5 mg/100g histamine and 1.6 mg/100g cadaverine. Those make certain safety during self-life but that purchased F market gradually increased biogenic amine during storage. Prepared salted mackerel of varying levels of freshness and processing methods were analyzed for biogenic amines. Salting process was performed using functional brine salt. VBN (volatile basic nitrogen) was used to establish freshness levels of salted mackerel. Analyses of mackerel muscle determined the values of freshness to be 9.2 mg/100g (good freshness), 18.2 mg/100g (bad freshness), respectively. After 40 days of storage, the content of histamine, cadaverine, putrescine in the freshness of salted mackerel was 2.0 mg/kg, 2.4 mg/kg and 0 mg/kg, respectively. The content of histamine, cadaverine, putrescine in salted mackerel of poor freshness was 71.3 mg/kg, 22.9 mg/kg and 17.8 mg/kg, respectively. It was concluded the presence of biogenic amines during the salting process of salted mackerel significantly effected freshness of materials. The presence of biogenic amines were detected in mackerel that underwent the salting process after 1 month of storage at $4{^{\circ}C}$. The levels of biogenic amines in the brine salted mackerel were higher than those found in dry, salted mackerel; however, the freshness of fish had an insignificant effect on biogenic amines. The presence of histamine was detected in small quantities in canned mackerel and mackerel pike from three companies. Alternatively, cadaverine and putrescine were not detected. Guamegi, vacuumed packed or sealed with a rope was purchased from three markets (A, B, C seafood company). Guamegi was stored and observed for 180 days at $-20{^{\circ}C}$. Histamine was detected in small quantities in all products stored at $-20{^{\circ}C}$. Levels of histamine was dependent on the types of packaging, rope packaging yielded the highest level. However, other amines were not detected.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.2
• /
• pp.689-692
• /
• 2015

Background: Incorporation of molecular analysis of the epidermal growth factor receptor (EGFR) gene into routine clinical practice has shown great promise to provide personalized therapy of the non-small cell lung cancer (NSCLC) in the developed world. However, the genetic testing of EGFR mutations has not yet become routine clinical practice in territories remote from the central regions of Russia. Therefore, we aimed to study the frequency of major types of activating mutations of the EGFR gene in NSCLC patients residing in West Siberia. Materials and Methods: We examined EGFR mutations in exons 19 and 21 in 147 NSCLC patients (excluding squamous cell lung carcinomas) by real time polymerase chain reaction. Results: EGFR mutations were detected in 28 of the 147 (19%) patients. There were 19 (13%) cases with mutations in exon 19 and 9 cases (6%) in exon 21. Mutations were more frequently observed in women (42%, p=0.000) than in men (1%). A significantly higher incidence of EGFR mutations was observed in bronchioloalveolar carcinomas (28%, p=0.019) and in adenocarcinomas (21%, p=0.024) than in large cell carcinomas, mixed adenocarcinomas, and NOS (4%). The EGFR mutation rate was much higher in never-smokers than in smokers: 38% vs. 3% (p=0.000). The frequency of EGFR mutations in the Kemerovo and Tomsk regions was 19%. Conclusions: The incorporation of molecular analysis of the EGFR gene into routine clinical practice will allow clinicians to provide personalised therapy, resulting in a significant increase in survival rates and improvement in life quality of advanced NSCLC patients.

• Korean Journal of Agricultural Science
• /
• v.18 no.2
• /
• pp.148-156
• /
• 1991

This study was carried out to investigate the storage conditions and methods required for commercialization of Jeung-Pyon. The storage characteristics of Jeung-Pyon were examined with Universal Testing Machine (UTM, Instron model 1000) for textural properties during storage of under the conditions such as sealing, nonsealing, and temperatures. The differences in storage characteristics on the different varieties and years of rices were not shown. When the Jeung-Pyon was lost it's quality as a commercial product, the measured value of UTM was increased as hardness 0.74Kg to 1.0Kg, gumminess 0.23Kg to 0.59Kg, chewiness 6Kg.mm to 18Kg.mm, but decreased in adhesiveness 0.65Kg.mm to 0.40Kg.mm, cohesiveness 0.61 to 0.34, springiness 41mm to 32mm. The nonsealed Jeung-Pyon was lost it's quality for storing at room temperature because of the case-hardening after 2 days. In the case of sealed Jeung-Pyon, the case-hardening phenomena by the evaporation of moisture was not occured, therefore it was a significant factor for an extension in sealed Jeung-Pyon. The shelf-life of sealed Jeung-Pyon was 20 days and 5 days respectively during storage at $-20^{\circ}C$ and $20^{\circ}C$. The sealed Jeung-Pyon stored at $5^{\circ}C$ was lost it's quality, in consideration of cold chain this temperature was suitable for retrogradation of starch. The Jeung-Pyon sealed with air and mosture proof packaging material stored at the accelerated condition as 92% R.H. and $35^{\circ}C$ lost it's quality within two or three days.

• Journal of the Korean Geographical Society
• /
• v.45 no.1
• /
• pp.11-25
• /
• 2010

Green Growth was declared as a national development agenda in 2008. There are many contributing concepts and factors in the process of molding the agenda, such as climate change, sustainable development, globalization, the so-called 747 campaign pledges by President Lee in 2007, and the hunger for economic growth in Asia and the Pacific. Green Growth is rather growth-oriented and pays less attention to environmental conservation and social justice. Green Development would fit better as the name of the agenda, dealing with the weaknesses of Green Growth. Climate change itself is a testing ground for geographic knowledges, whose demand is growing rapidly. The contemporary issue increasingly bears complexity that Earth System Science and Sustainability Science have emerged as a research and applications program. Geography is widely recognized as a portal to these programs, where inter- and trans-disciplinary studies are required. Regional potentials should be evaluated from a holistic view so that proper development goals are chosen. Different development trajectory should be taken, depending on the amount of potential a region bears. Material loop should be closed for environmentally sound regions. Green way of life is essential for low carbon society. In the circumstances of climate change in Korean Peninsula and needing of energy efficiency, geographic insight or imagination is urgent for Green Development.

• KSBB Journal
• /
• v.30 no.2
• /
• pp.58-62
• /
• 2015

A 3-weeks feeding trial with 288 laying hens were conducted to determine the efficacy of lecithin-free egg yolk at different levels of dietary Ca on performance and Ca absorption. Laying hens were divided into 6 groups according to calcium level and testing agent; 0% calcium feed (A), 0.2% calcium feed (B), 0.4% calcium feed (C, normal feed), 0.6% calcium feed (D), 0.4% calcium feed + 0.2% egg byproduct (C+0.2), 0.4% calcium feed + 0.4% egg byproduct (C+0.4). The final body weight gain of C+0.2 and C+0.4 groups were higher by 1.5% and 7.4% respectively than group C. Tibia ash contents did not show significantly difference, but calcium contents increase (p<0.05) in C+0.2 and C+0.4 groups. Parallel undecalcified tibia joint sections were stained for calcium absorption by the von Kossa's stain. This result show that lecithin free egg byproduct supplementation to normal calcium feed improved growth performance and calcium utilization in laying hens.

• The Journal of Advanced Prosthodontics
• /
• v.1 no.3
• /
• pp.113-117
• /
• 2009

STATEMENT OF PROBLEM. Recently Yttrium-stabilized tetragonal zirconia polycrystal (Y-TZP) has been introduced due to superior flexural strength and fracture toughness compared to other dental ceramic systems. Although zirconia has outstanding mechanical properties, the phenomenon of decrease in the life-time of zirconia resulted from degradation in flexural strength after low temperature aging has been reported. PURPOSE. The objective of this study was to investigate degradation of flexural strength of Y-TZP ceramics after various low temperature aging treatments and to evaluate the phase stability and micro-structural change after aging by using X-ray diffraction analysis and a scanning electron microscope (SEM). MATERIAL AND METHODS. Y-TZP blocks of Vita In-Ceram YZ (Vita Zahnfabrik, Bad $S\ddot{a}ckingen$, Germany) were prepared in 40 mm (length) $\times$ 4 mm (width) $\times$ 3 mm (height) samples. Specimens were artificially aged in distilled water by heat-treatment at a temperature of 75, 100, 125, 150, 175, 200, and $225^{\circ}C$ for 10 hours, in order to induce the phase transformation at the surface. To measure the mechanical property, the specimens were subjected to a four-point bending test using a universal testing machine (Instron model 3365; Instron, Canton, Mass, USA). In addition, X-ray diffraction analysis (DMAX 2500; Rigaku, Tokyo, Japan) and SEM (Hitachi s4700; Jeol Ltd, Tokyo, Japan) were performed to estimate the phase transformation. The statistical analysis was done using SAS 9.1.3 (SAS institute, USA). The flexural strength data of the experimental groups were analyzed by one-way analysis of variance and to detect statistically significant differences ($\alpha$= .05). RESULTS. The mean flexural strength of sintered Vita In-Ceram YZ without autoclaving was 798 MPa. When applied aging temperature at below $125^{\circ}C$ for 10 hours, the flexural strength of Vita In-Ceram YZ increased up to 1,161 MPa. However, at above $150^{\circ}C$, the flexural strength started to decrease. Although low temperature aging caused the tetragonal-to-monoclinic phase transformation related to temperature, the minimum flexural strength was above 700 MPa. CONCLUSION. The monoclinic phase started to appear after aging treatment above $100^{\circ}C$. With the higher aging temperature, the fraction of monoclinic phase increased. The ratio of monoclinic/tetragonal + monoclinic phase reached a plateau value, circa 75% above $175^{\circ}C$. The point of monoclinic concentration at which the flexural strength begins to decrease was between 12% and 54%.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.6 no.8
• /
• pp.1352-1357
• /
• 2002

This Paper describes algorithm to extract license plates in vehicle images. Conventional methods perform preprocessing on the entire vehicle image to produce the edge image and binarize it. Hough transform is applied to the binary image to find horizontal and vertical lines, and the license plate area is extracted using the characteristics of license plates. Problems with this approach are that real-time processing is not feasible due to long processing time and that the license plate area is not extracted when lighting is irregular such as at night or when the plate boundary does not show up in the image. This research uses the gray level transition characteristics of license plates to verify the digit area by examining the digit width and the level difference between the background area the digit area, and then extracts the plate area by testing the distance between the verified digits. This research solves the problem of failure in extracting the license plates due to degraded plate boundary as in the conventional methods and resolves the problem of the time requirement by processing the real time such that practical application is possible. This paper Presents a power automated license plate recognition system, which is able to read license numbers of cars, even under circumstances, which are far from ideal. In a real-life test, the percentage of rejected plates wan 13%, whereas 0.4% of the plates were misclassified. Suggestions for further improvements are given.

• Culinary science and hospitality research
• /
• v.18 no.3
• /
• pp.18-31
• /
• 2012

The current study aimed to analyze data conducting a survey with 211 females in Ulsan Gyeongnam province in order to understand what influence lifestyle types of female customers exert on eating-out behaviors from the perspective of eating-out motivation. So as to achieve research purpose, we conducted factor analysis, reliability analysis, t-test, ANOVA analysis, multiple regression analysis, correlation analysis and frequency analysis through SPSS 12.0 program. Findings of analyses indicated that items of lifestyle involve d 4 factors, and the item of eating out behaviors was extracted to be 1 factor. As a result of testing differences in lifestyle depending on residential areas, both type of chasing fashion and type of extroverted dependence have significant differences in variables, and both factors indicated that females living in Ulsan have higher mean values than those of females living in Gyeongnam. As a result of analyzing lifestyle depending on age, which was adopted in part, females at the ages of 31~40 had higher mean values than those in other ages in the type of chasing fashion, and females above the age of 51 had higher mean values than those in other ages in type of chasing health. As a result of lifestyle depending on academic backgrounds, university students or graduates had higher mean values than those in other academic backgrounds. For the influence of lifestyle types on eating-out behaviors depending on eating-out motivation, the type of chasing fashion, the type of purchasing the quality of life, and the type of chasing health were p<0.001, having significant effects.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.48 no.2
• /
• pp.127-134
• /
• 2020

High supersonic aircraft are exposed to high temperature environments by aerodynamic heating during supersonic flight. Thermal protection system structures such as double-panel structures are used on the skin of the fuselage and wings to prevent the transfer of high heat into the interior of an aircraft. The thin-walled double-panel skin can be exposed to acoustic loads by supersonic aircraft's high power engine noise and jet flow noise, which can cause sonic fatigue damage. Therefore, it is necessary to examine the behavior of supersonic aircraft skin structure under thermal-acoustic load and to predict fatigue life. In this paper, we designed and fabricated thermal-acoustic test equipment to simulate thermal-acoustic load. Thermal-acoustic testing of the titanium specimen under thermal-acoustic load was performed. The analytical model was verified by comparing the thermal-acoustic test results with the finite element analysis results.