• Title/Summary/Keyword: Life Conditions

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Molecular Cloning of the cDNA of Heat Shock Protein 88 Gene from the Entomopathogenic Fungus, Paecilomyces tenuipes Jocheon-1

  • Liu, Ya-Qi;Park, Nam Sook;Kim, Yong Gyun;Kim, Keun Ki;Park, Hyun Chul;Son, Hong Joo;Hong, Chang Ho;Lee, Sang Mong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.28 no.2
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    • pp.71-84
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    • 2014
  • The full-length heat shock protein 88 (HSP88) complementary DNA (cDNA) of Paecilomyces tenuipes Jocheon-1 was obtained by screening the Paecilomyces tenuipes (P. tenuipes) Jocheon-1 Uni-Zap cDNA library and performing 5' RACE polymerase chain reaction (PCR). The P. tenuipes Jocheon-1 HSP88 cDNA contained an open reading frame (ORF) of 2,139-basepair encoding 713 amino acid residues. The deduced amino acid sequence of the P. tenuipe s Jocheon-1 HSP88 cDNA showed 77% identity to Nectria haematococca HSP88 and 45-76% identity to other fungal homologous HSP88s. Phylogenetic analysis and BLAST program analysis confirmed that the deduced amino acid sequences of the P. tenuipes Jocheon-1 HSP88 gene belonged to the ascomycetes group within the fungal clade. The P. tenuipes Jocheon-1 HSP88 also contained the conserved ATPase domain at the N-terminal region. The cDNA encoding P. tenuipes Jocheon-1 HSP88 was expressed as an 88 kilodalton (kDa) polypeptide in baculovirus-infected insect Sf9 cells. Under higher temperature conditions for the growth of the entomopathogenic fungus, mRNA expression of P. tenuipes Jocheon-1 HSP88 was quantified by real time PCR (qPCR). The results showed that heat shock stress induced a higher level of mRNA expression compared to normal growth conditions.

Conditions for Artificial Culture of Lemna Paucicostata and Potentiality as an Alternative Biomass Source (바이오매스 자원으로서의 Lemna Paucicostata의 인공배양조건과 이화학적 특성에 관한 연구)

  • Kwag, Jung-Hoon;Lee, Jin-Eui;Kim, Ki-Hye;Eum, Hye-Yeong;Shin, Jong-Suh;Ra, Chang-Six
    • Journal of Animal Environmental Science
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    • v.16 no.2
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    • pp.143-152
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    • 2010
  • Conditions for artificial culture of Lemna Paucicostata and its nutritional values were examined in this study. Lemna P. was cultured using artificial wastewater and a bioreactor (total volume $2,630\;cm^3$, working volume $2,240\;cm^3$) was operated at conditions of 6,250 lux and $28^{\circ}C$. Water flow affected the growth of Lemna P.: growth rate was very high (more than $1.1\;d^{-1}$) at a condition of no-water movement, but it was very low (less than $0.15\;d^{-1}$) when water moved slowly. The growth of Lemna P. was higher in $16h\;d^{-1}$ light cycle than in Sand $24h\;d^{-1}$, and it was also severely affected by the initial $NH_4$-N levels of wastewater. The growth rate of Lemna P. was high in lower $NH_4$-N level, indicating that the growth rate is in inverse proportion to $NH_4$-N concentration in wastewater. However, the contents of crude protein (CP) of Lemna P. were proportional to the initial $NH_4$-N concentration. The CP contents of Lemna P. cultured at 2, 10, 50 and 100 $NH_4$-N mg $L^{-1}$ was 18, 24, 37, 43%, respectively, showing the Lemna P. cultured at 50 and $100\;mg\;L^{-1}$ had similar protein contents to linseed (CP 35%), cottonseed (CP 38%) and soybean (CP 45%). Fat, protein, fiber, NDF and ADF contents of Lemna P. harvested at conditions of $16h\;d^{-1}$ light cycle and less than $2\;mg\;L^{-1}$ of $NH_4$-N level was 2.8, 18, 27, 20, 41 and 65.7%, respectively. Since the growth rate of Lemna P. was very high (more than $1.1\;d^{-1}$) at those conditions, it was convinced that mass production of valuable protein and fiber sources are feasible. In particular, since the Lemna P. has unsaturated fatty acids found mainly in animal fat as well as beneficial fatty acids to health such as C18:ln9c, C18:2n6c, C20:5n3 and C22:2, the Lemna P. biomass would be a highly valuable alternative feed source to grains.

A Study on Stroke Patients' ADL, Depression, Self-Efficacy and Quality of Life (재가 뇌졸중 환자의 일상활동 수행능력, 우울, 자기효능감 및 삶의 질과의 관계)

  • Cho, Bok-Hee;Ko, Mi-Hye;Kim, Soon-Young
    • The Korean Journal of Rehabilitation Nursing
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    • v.6 no.1
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    • pp.51-60
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    • 2003
  • This study was a descriptive research in search of a nursing intervention scheme to improve stroke patients' quality of life by understanding the relationship of stroke patients' ADL, depression, self-efficacy and quality of life with various variables and identifying factors that affect their quality of life. Each subject was interviewed one to one based on a structured questionnaire. With regard to research tools, Modified Barthel Index (MBI: Fortinsky et al., 1981), which was translated by Choi, Hye-sook (1996), was used in measuring ADL, Randloff's (1977) tool, which was translated by Choi, Soon-hee, was used in measuring depression, and the tool developed by Sherer et al. (1982), which is to measures self-efficacy under general conditions not limited to specific conditions, and modified by O, Bok-ja (1994) was used in measuring self-efficacy. The quality of life was measured using the scale of satisfaction of life developed by Diener et al. (1985). The results of this study were as follows: 1. The means of ADL of the subjects was $79.5{\pm}31.9$, depression $26.8{\pm}10.4$, self-efficacy $47.1{\pm}25.7$, and the quality of life $12.3{\pm}4.9$. 2. The subjects' quality of life showed a statistically significant difference according to gender (t=7.9, p= .006), satisfaction with income (F=5.8, p= .004), the burden of medical fee (F=3.7, p= .028) and the period of disease (F=2.8, p= .042). 3. With regard to relationship among ADL, depression, self-efficacy and the quality of life, ADL was in a relatively low positive correlation (r= .293, p= .003) with and the quality of life, depression in a high negative correlation (r=- .634, p= .000) with the quality of life, and self-efficacy in a positive correlation with the quality of life (r= .388, p= .000). 4. Factors that made a significant influence on the quality of life were depression (B=- .309, p= .001) and satisfaction with income (B=-2.611, p= .001). Based on these results, this study made following suggestions: 1. It is necessary to run rehabilitation programs to improve stroke patients' ADL, depression and self-efficacy. 2. It is necessary to perform research of monitoring stroke patients' quality of life in various areas using measuring tools.

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Living Conditions and Life Satisfaction of Single-person Households by Life Cycle : An Analysis of Single-person Households in Kimpo, South Korea (1인가구의 생애주기별 생활실태 및 생활만족도 : 김포시 1인가구를 중심으로)

  • Kim, Jung Eun;Park, Jeongyun;Seo, Jiwon;Song, Hyerim
    • Journal of Family Resource Management and Policy Review
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    • v.27 no.3
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    • pp.21-37
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    • 2023
  • This study examines the living conditions and life satisfaction of single-person households according to their life cycle. The survey was conducted from August to October 2022, and respondents were single-person households from Kimpo, South Korea. The respondents were categorized to three groups namely, young, middle-aged, and older adults by life cycle, and items regarding their sociodemographic background, personal life, family relations, and life satisfaction were included in the analysis. Descriptive statistics, Chi-square analysis, analysis of variance, and multiple regression analysis were performed. The key findings were as follows. First, significant differences were found according to life cycle in the respondents' diet, clothing, housing, financial and home management, self-care, and leisure life. Second, the variables that significantly affected the level of life satisfaction of single-person households were the life cycle with young adults showing a higher level of satisfaction; having a family member to care; living alone voluntarily, discrimination experience; community awareness; and ties with the local community. Based on the results, it is clear that single-person households have different needs and problems in each stage of life cycle. Thus, to reflect the different experiences and needs of single-person households by life cycle, tailored policy and programs should be provided for young, middle-aged, and older adult single-person households.

Counseling Services and Work Conditions Perceived by Counselors in Healthy Family Support Centers (상담실무자가 지각한 건강가정지원센터의 상담업무와 근무환경)

  • Lim, Choon-Hee
    • Journal of Families and Better Life
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    • v.25 no.5
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    • pp.83-105
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    • 2007
  • The present study investigated counseling services and work conditions perceived by counselors in Healthy Family Support Centers. Questionnaire survey by mail was used to collect data from 32 counselors in Healthy Family Support Centers. Major findings of this study were as follows. First, many counselors in Healthy Family Support Centers perceived work load of counseling services heavily though they thought counseling work attractive and they would keep on working. Second, many counselors had a positive view of work conditions as a workplace but not a few of them complained about physical work conditions including poor facilities like lack of counseling room space in Centers. In addition to this, many suggestions were proposed to improve the counseling services and work conditions perceived by counselors in Healthy Family Support Centers.

A Study on Conditions for Effective Home Management: Relations to Resourcefulness nd Communication (효과적 가정관리 조건에 관한연구: 자원 유용성 및 의사소통과의 관계를 중심으로)

  • 한경미
    • Journal of Families and Better Life
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    • v.17 no.2
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    • pp.105-120
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    • 1999
  • The purpose of this study was to examine dimensions related to effective managerial actions in association with effects of resourcefulness personal and managerial communications and condition s for effective home management on the basis of managerial process. Including elements of managerial process conditions for effective home management were divided into the four factors performance-condition dichotomy timing high level and middle range. In conclusion first the managerial process for effective management can be classified into the four conditions related to each other. That was termed as 'conditions for effective home management' Second the most influential variables on conditions for effective home management were personal and managerial communication. The four conditions for effective home management need to include communicaion. Third resuorcefulness was a crucial factor in relation to home management resources division. It is recommedned that resuorcefulness needs to de elop managerial actions of housewives.

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Characteristics of Remodeling in Apartment Housing according to the Building Life-cycle and Family Life-cycle (건물의 생애주기와 가족생활주기에 따른 공동주택의 개조특성)

  • 윤정숙;정유선
    • Proceeding of Spring/Autumn Annual Conference of KHA
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    • 2000.11b
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    • pp.123-129
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    • 2000
  • The purpose of this study was to provide the fundamental data for the effective housing remodeling plan. Through the questionnaire survey, the actual conditions of remodeling in apartment housing were investigated. And as the result of statistic analysis, the relationships between characteristics of remodeling and building life-cycle, and family life-stage, were found out. The major findings of this study are as follows; 1) Most of apartment residents have remodeled the family living space and private living space. In detail, they performed remodeling frequently the change of finishing materials, facilities and equipments, and the change of the location of curtain wall & storage fixtures. 2) It might be predicted that the building life-cycle was influential variable to predict apartment residents' remodeling. Therefore, in planning the support service for the remodeling, the building life-cycle should be taken into consideration.

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The Predicton of the Shelf-life of Packaged Meals (Kim Pab) Marketed in Covenience Stores Using Simulation Study (모의실험을 통한 편의점 판매용 김밥 도시락의 유통기한 예측)

  • 곽동경;김성희
    • Journal of Food Hygiene and Safety
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    • v.11 no.3
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    • pp.189-196
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    • 1996
  • Based on microbiological-chemical test results under simulated time-temperature conditions, total plate count, coliform, and volatile basic nitrogen were selected as effective quality indicators for estimating probable shelf-life on Kim Pab dosirak marketed in convenience stores, and shelf-life at each storage temperature was calculated from regression equation between effective quality indicator standard limit and storage time. Estimated shelf lives of Kim Pab Dosirak were 17, 3 and 2 hours respectively under 1$0^{\circ}C$, 2$0^{\circ}C$ and 3$0^{\circ}C$. Because shelf-life was especially affected by intial values, regression analysis between initial effective quality indicator values and storage hours was performed for the estimation of probable shelf-life on Kim Pab during storage at 10, 20, or 3$0^{\circ}C$.

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Evaluation of Tool Life for Forging Die due to Lubricants and Suface Treatments (단조 금형의 윤활, 표면처리 및 금형 수명 평가)

  • 김병민
    • Transactions of Materials Processing
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    • v.11 no.3
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    • pp.211-216
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    • 2002
  • The mechanical and thermal load, and thermal softening occuring by the rush temperature of die, in warm and hot forging, cause wear, heat cracking and plastic deformation, etc. This paper describes the effects of solid lubricants and surface treatments for warm forging die. Because cooling effect and low friction are essential to the long life of dies, optimal surface treatments and lubricants are very important to hot and warm forging process. The main factors affecting die hardness and heat transfer, are surface treatments and lubricants, which are related to heat transfer coefficient, etc. To verify the effects, experiments are performed for heat transfer coefficient in various conditions - different initial billet temperatures and different loads. Carbonitriding and ionitriding are used as surface treatments, and oil-base and water-base graphite lubricants are used. The effects of lubricant and surface treatment for warm and hot forging die life are explained by their thermal characteristics, and the new developed technique in this study for predicting tool life can give more feasible means to improve the tool life in hot forging process.