• Journal of the Korea Safety Management & Science
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• v.2 no.2
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• pp.71-83
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• 2000

This paper presents accelerated life tests for Type I censoring data under probabilistic stresses. Probabilistic stress, $S_j$, is the random variable for stress influenced by test environments, test equipments, sampling devices and use conditions. The hazard rate, ,$theta_j$, is the random variable of environments and the function of probabilistic stress. Also it is assumed that the general stress distribution is uniform, the life distribution for the given hazard rate, $\theta$, is exponential and inverse power law model holds. In this paper, we obtained maximum likelihood estimators of model parameters and the mean life in use stress condition.

• Bulletin of the Korean Chemical Society
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• v.34 no.9
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• pp.2663-2670
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• 2013

The purpose of stability testing is to provide evidence about how the quality of a drug varies with time under the influence of a variety of environmental factors. In this study, erythropoietin (EPO) was analyzed under different pH (pH 3 and pH 9) and temperature ($25^{\circ}C$ and $40^{\circ}C$) conditions according to current Good Manufacturing Practice (cGMP) and International Conference on Harmonisation (ICH) guidelines. The molecular weight difference between intact EPO and deglycosylated EPO was determined by SDS-PAGE, and aggregated forms of EPO under thermal stress and high-pH conditions were investigated by size exclusion chromatography. High pH and high temperature induced increases in dimer and high molecular weight aggregate forms of EPO. UPLC-ESI-TOF-MS was applied to analyze the changed modification sites on EPO. Further, normal-phase high-performance liquid chromatography was performed to identify proposed glycan structures and high pH anion exchange chromatography was carried out to investigate any change in carbohydrate composition. The results demonstrated that there were no changes in modification sites or the glycan structure under severe conditions; however, the number of dimers and aggregates increased at $40^{\circ}C$ and pH 9, respectively.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.12 s.177
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• pp.149-155
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• 2005

The rotating components such as turbine rotors in service are generally subjected to multiaxial cyclic loading conditions. The prediction of fatigue lift for turbine rotor components under complex multiaxial loading conditions is very important to prevent the fatigue failures in service. In this paper, axial and torsional low cycle fatigue tests were preformed for 3.5NiCrMo steels serviced low pressure turbine rotor of nuclear power plant. Several methods to predict biaxial fatigue life such as Tresca, von Mises and Brown & Miller's critical plane approach were evaluated to correlate the experimental results for serviced NiCrMoV steel. The fracture mode and fatigue characteristics of NiCrMoV steel were discussed based on the results of fatigue tests performed under the axial and torsional test conditions. In particular, the Brown and Miller's critical plane approach was found to best correlate the experimental data with predictions being within a factor of 2.

• ALGAE
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• v.33 no.2
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• pp.181-189
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• 2018

Emiliania huxleyi (a haptophyte) is the most abundant coccolithophore species that produces delicate calcite scales called coccoliths. In this study, we identified several candidate genes associated with coccolith production by comparing the transcriptomes of the calcifying (CCMP 371) and non-calcifying (CCMP 2090) strains of E. huxleyi. Among the candidates, genes highly expressed in CCMP 371 were identified. To confirm whether these genes are associated with calcification, we modulated coccolith production in CCMP 371 by culturing it at different calcium concentrations. At an ambient (10 mM) concentration of calcium in the growth medium, CCMP 371 sustained its calcifying ability. However, at a low (0.1 mM) concentration or absence of calcium, there was no calcite formation, demonstrating that calcium-limiting conditions negatively affect calcification. We also evaluated the expression patterns of the putative genes in cells grown at different calcium concentrations by quantitative reverse transcription polymerase chain reaction. In addition, we showed that the growth rate of cells cultured under calcium-limiting conditions does not differ from that under ambient conditions. Further studies are required to investigate the roles of the putative calcification-associated genes at the molecular level.

• Korean journal of food and cookery science
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• v.6 no.2
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• pp.105-119
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• 1990

A study on the dietary habit and the food perference of Pre-school Children was made in order to identify the state of their dietary life and provide the data of dietetic education which can help the children to live a desirable food life, by analysing the present dietry life into the dietry habits, food preference, the actual condition of nutrition intake and table manners. 1. The Diet Habits. Both boys and girls were fond of dinner best and tended to neglect breakfast and lunch. 64.2% of the total children took meals regularly. 2. Food Preference. The most favorite food was beef, ham, Dupoo (beancurd), squid, ice-cream, bananas, watermelon, sweet potatoes, corn, Ingeolmi (cake form glutinous rice), doughnuts, JJajangmyon (Chinese noodles), Coke, fried chicken. 3. The Actual Conditions of Nutrition Intake. The frequency of intake of meat and its processed food was the lowest and that of fishes, shellfishes, fats and vegetables was low, too. 4. Table manners and Sanitary Conditions. The average mark of table manners and sanitary conditions is 12.49${\pm}$2.65. The children didn't practice well in the following points; talking at table, beginning to eat after the beginning of elder person's, brushing their teeth after taking meals, keeping a straight posture.

• Journal of Microbiology and Biotechnology
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• v.14 no.4
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• pp.859-862
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• 2004

An isolate of Pseudomonas putida has been found to aggressively colonize root tips and induce plant resistance to Fusarium wilt. However, P. putida mutants lacking Fe-superoxide dismutase (SOD) or both FeSOD and MnSOD activities are less competitive in root tip colonization. In the current study, the growth of an FeSOD mutant was found to be more sensitive than that of the wild-type or a MnSOD mutant to oxidative stress imposed by paraquat treatment and culturing with the soil fungus Talaromyces flavus, which generates reactive oxygen species. Also, the loss of culturability with an aging stationary-phase culture was greater for a double SOD mutant than an FeSOD mutant, while no reduction in culturability was observed with the wild-type and a MnSOD mutant under the same protracted stationary-phase conditions. Accordingly, it was concluded that FeSOD activity is the major form of SOD in P. putida and plays an essential role in survival under stress conditions when increased oxidative stress is encountered.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.5
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• pp.400-407
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• 2011

The stress distribution and stress amplitude of a membrane are major factors to decide the mechanical fatigue life of PEMFC (Polymer Electrolyte Membrane Fuel Cell). In this paper, mechanical stresses under operating hygro-thermal condition of the membrane are numerically modelled. Contact analysis between gas diffusion layer (GDL) and the membrane is performed under various temperature-humidity conditions. The structural model has nonlinear material properties depending on temperature and relative humidity. Several geometric conditions are applied to the model. The numerical analysis results indicate that deformations of the membrane are strongly related with assembly conditions of the fuel cell. The fatigue life is predicted for practical operating condition through experimental data.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2017.05a
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• pp.148-153
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• 2017

During natural ageing of HTPB propellant undergoes a series of slow physico-chemical degradation reactions. By using accelerated ageing conditions it is possible to simulate the material behaviour at different time-temperature conditions especially focused on the in-service conditions. Ageing behaviour of HTPB propellant were investigated using HFC(Micro-Heat Flow Calorimeter) is universal technique for measuring the rate of slow chemical and physical processes in long-term storage.

• Journal of Life Science
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• v.20 no.7
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• pp.999-1004
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• 2010

Investigations were carried out on a $\beta$-glucosidase produced by Aspergillus niger under solid-state fermentation conditions as a model of enzyme recovery from fermented wheat bran. The leaching efficiency of distilled water to recover the enzyme from the fermented bran was higher than acetate buffer, citrate buffer, citrate-phosphate buffer and 5% methanol; thus, the conditions were further optimized with distilled water as the extracting agent. After fermented bran was washed three times with distilled water for 1.5 hr each under shaking conditions at 1:5 solid to solvent ratio, a maximum recovery of 0.025 U/g of wheat bran was obtained.

• Journal of Applied Biological Chemistry
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• v.59 no.2
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• pp.99-102
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• 2016

The phototactic behavioral responses of Tribolium castaneum adults to light-emitting diodes (LEDs) of seven different wavelengths were determined under various conditions (light exposure times, light sources, and luminance intensities) and compared with those of a black light bulb (BLB) under laboratory conditions. Based on the attractive rate (%) of T. castaneum adults under optimal conditions (50 lx and an 48 h exposure time) in the dark, red LED ($625{\pm}10nm$) exhibited the highest potential attractive rate (97.8 %), followed by yellow ($590{\pm}5nm$, 68.9 %), green ($520{\pm}5nm$, 55.6 %), infrared (IR) (730 nm, 54.4 %), white (450-620 nm, 41.1 %), blue ($470{\pm}10nm$, 34.4 %), and ultraviolet (UV) (365 nm, 0.06 %) LEDs. In comparison, red LED (97.8 %) was approximately 3.4 times more attractive to T. castaneum adults than the BLB (28.9 %). These results indicate that a red LED trap could be useful to control T. castaneum adults.