• Animal cells and systems
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• 제3권3호
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• pp.311-317
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• 1999

Prolactin (PRL) was reported to be locally synthesized in many brain areas including the hypothalamus, thalamus (TH) and hippocampus (HIP). In the pituitary lactotrophs, PRL synthesis is dependent upon a pituitary-specific transcription factor, Pit-1. In the present study, we attempted to identify Pit-1 or Pit-1-like protein in brain areas known as the synthetic sites of PRL. Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis showed the same Pit-1 transcripts in brain areas such as the medial basal hypothalamus (MBH), preoptic area (POA), TH, and HIP with the Pit-1 transcripts in the anterior pituitary (AP). Electrophoretic mobility shift assay (EMSA) was run with nuclear protein extracts from brain tissues using a double strand oligomer probe containing a putative Pit-1 binding domain. Shifted bands were found in EMSA results with nuclear proteins from MBH, POA, TH and HIP. Specific binding of the Pit-1-like protein was further confirmed by competition with an unlabeled cold probe. Antisense Pit-1 oligodeoxynucleotide (Pit-1 ODN), which was designed to bind to the Pit-1 translation initiation site and block Pit-1 biosynthesis, was used to test Pit-1 dependent brain PRL transcription. Two nmol of Pit-1 ODN was introduced into the lateral ventricle of a 60-day old male rat brain. RNA blot hybridization and in situ hybridization indicated a decrease of PRL mRNA signals by the treatment of Pit-1 ODN. Taken together, the present study suggests that Pit-1 may play an important role in the transcriptional regulation of local PRL synthesis in the brain.

• 한국음향학회지
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• 제16권4호
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• pp.76-84
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• 1997

본 논문은 이동통신기기의 핵심부품중 하나인 VCO를 IC로 설계한 내용을 기술하였다. 설계한 VCO는 배리캡을 사용한 LC 동조형발진기로 구현하였다. 사용한 발진소자중 인덕터는 실리콘 IC 구현상의 난점[8]으로 인해 외부로 구성하고 나머지부분을 모두 IC화 하였다. 제작하는데 사용된 마스크 수는 15개이며 칩 사이즈는 1150um${\times}$780um이다. 제작한 VCO IC를 테스트한 결과 전원전압 5V에서 제어전압을 1V에서 3V로 변화시킬때 880MHz 영역에서 동작하였으며 주파수 천이는 425KHz/V, 주파수 편이는 1.97MHz/T, 캐리어 레벨은 -7dBm, 전류소모는 16.7mA이었다. 또한, 위상 잡음은 50KHz 오프셋에서 -80dBc/Hz 이며 중심주파수에 대한 하모닉응답은 -41dBm 이다. 향후 송수신단을 단일 칩화하기 위해서는 외부회로도 실리콘 기판위에 구현할 수 있는 실리콘 MMIC[1][8]에 대한 연구가 수행되어야 할 것이다.

• 가정과삶의질연구
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• 제23권2호
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• pp.37-47
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• 2005

Korea is having entered the Ageing Society since 2000 and expected to be the Aged Society by 2019. Proliferation of the nuclear families, attitude shift of supporting the senior people and higher participation of married women in public life as well as the steadily increasing senior population have caused the need for development of the facilities or housings for the senior people. This study is based on the assumption that the congregate housing as one of the options for those senior people who are healthy enough do not need the nursing home. For the questionnaire survey, the preference of 500 Korean pre-senior people on spatial planning elements of units for the senior congregate housing was investigated. The collected data are analysed using SPSS 10.0 for Windows to deduce frequency, percentage, average, Chi-squared test with cross-tabulations. The results of this study were as follows; Most of respondents preferred to the size of 11-15pyung(for single), 21-25pyung(for couple). The type of spatial composition was preferred one-room type(for single), 1-2 bedroom type with kitchen (for couple). And they preferred L/DK type. Also, they preferred furnished units not to bring their own furniture when relocating to the senior congregate housing, which was somewhat different result from previous studies. There were significant differences between preferred spatial planning elements and gender, income, and level of education.

• 한국전기전자재료학회논문지
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• 제28권5호
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• pp.291-294
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• 2015

Recently, ZnO based oxide TFTs used in the flexible and transparent display devices are widely studied. To apply to OLED display switching devices, electrical performance and stability are important issues. In this study, to improve these electrical properties, we fabricated TFTs having Al doped Zinc Oxide (AZO) layer inserted between the gate insulator and ZnO layer. The AZO and ZnO layers are deposited by Atomic layer deposition (ALD) method. I-V transfer characteristics and stability of the suggested devices are investigated under the positive gate bias condition while the channel defects are also analyzed by the photoluminescence spectrum. The TFTs with AZO layer show lower threshold voltage ($V_{th}$) and superior sub-threshold slop. In the case of $V_{th}$ shift after positive gate bias stress, the stability is also better than that of ZnO channel TFTs. This improvement is thought to be caused by the reduced defect density in AZO/ZnO stack devices, which can be confirmed by the photoluminescence spectrum analysis results where the defect related deep level emission of AZO is lower than that of ZnO layer.

• 환경영향평가
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• 제14권4호
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• pp.179-193
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• 2005

As the world's attention turns to sustainability and the considerations of cumulative effects, the concept of Strategic Environmental Assessment(SEA) has become more significant and urgent and increasing number of countries and international organizations now undertake some forms of SEA. The term SEA, however, is variously defined and understood; generally it means a formal process of systematic analysis of the environmental effects on development policies, plans, programmes and other proposed strategic actions. This process extends the aims and principles of EIA upstream in the decision-making process, beyond the project level in which major alternatives are still open. There is a shift toward more integrative approaches and greater use of Environmental Impact Assessment (EIA) and Strategic Environmental Assessment (SEA) as sustainability tools in cooperation with Environmental Management System (EMS). Currently, Korea has EIA system and Prior Environmental Review System (PERS) which is different type of SEA as Environment Assessment (EA) system. APEMI IA MODEL integrated following three pillar(refer to attached figure.1) ; First pillar symbolized decision making cycle with planning process. Second pillar symbolized integrated assessment which tying SEA and EIA with specific impacts assessment(eg: social impact assessment, economic impact assessment, health impact assessment etc) in cooperation with EMS. Third pillar symbolized EA best practical procedure of International Association for Impact Assessment(IAIA). Considering the above, we applied new conceptual model(APEMI IA MODEL) into Impact Assessment for better integrated decision-making in KOREA as an alternative IA system(IS IA MODEL A and B refer to attached figure 4, 5).

• Asian-Australasian Journal of Animal Sciences
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• 제27권8호
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• pp.1088-1097
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• 2014

The study aimed to investigate the effects of gynosaponin on in vitro methanogenesis under different forage-concentrate ratios (F:C ratios). Experiment was conducted with two kinds of F:C ratios (F:C = 7:3 and F:C = 3:7) and gynosaponin addition (0 mg and 16 mg) in a $2{\times}2$ double factorial design. In the presence of gynosaponin, methane production and acetate concentration were significantly decreased, whereas concentration of propionate tended to be increased resulting in a significant reduction (p<0.05) of acetate:propionate ratio (A:P ratio), in high-forage substrate. Gynosaponin treatment increased (p<0.05) the butyrate concentration in both F:C ratios. Denaturing gradient gel electrophoresis (DGGE) analysis showed there was no apparent shift in the composition of total bacteria, protozoa and methanogens after treated by gynosaponin under both F:C ratios. The real-time polymerase chain reaction (PCR) analysis indicated that variable F:C ratios significantly affected the abundances of Fibrobacter succinogenes, Rumninococcus flavefaciens, total fungi and counts of protozoa (p<0.05), but did not affect the mcrA gene copies of methanogens and abundance of total bacteria. Counts of protozoa and abundance of F.succinogenes were decreased significantly (p<0.05), whereas mcrA gene copies of methanogens were decreased slightly (p<0.10) in high-forage substrate after treated by gynosaponin. However, gynosaponin treatment under high-concentrate level did not affect the methanogenesis, fermentation characteristics and tested microbes. Accordingly, overall results suggested that gynosaponin supplementation reduced the in vitro methanogenesis and improved rumen fermentation under highforage condition by changing the abundances of related rumen microbes.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.1749-1753
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• 2003

We proposed several systems for 1Giga bit Modem. The first, Binary ASK(Amplitude Shift Keying) system has a high speed shutter transmitter and no IF(Intermediate Frequency) receiver only by symbol synchronization. The advantage of proposed system is that circuitry is very simple without IF process. The disadvantage of proposed system are that line spectrum occurs interference to other channels, and enhancement to 4-level system is impossible due to its large SNR degradation. The second, Binary phase modulation system has a high speed shutter transmitter and IF-VCO(IF-Voltage Controlled Oscillator) control by base-band phase rotation. Polarity of shutter window is changed by the binary data. The window should be narrow same as above ASK. The advantage of proposed system is which error rate performance is superior. The disadvantage of proposed system are that Circuitry is more complex, narrow pull-in range of receiver caused by VCO and spectrum divergence by the non-linear amplifier. The third, 4-QAM(Quadrature Amplitude Modulation)system has a nyquist pulse transmitter and IF-VCO control by symbol clock. The advantage of proposed system are that signal frequency band is a half of 1GHz, reliable pull-in of VCO and possibility of double speed transmission(2Gbps) by keeping 1GHz frequency-band. The disadvantage of proposed system are that circuit complexity of pulse shaping and spectrum divergence by the non-linear amplifier.

• 간호행정학회지
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• 제22권3호
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• pp.209-219
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• 2016

Purpose: To analyze the proportion of medical institutions meeting the legal standard for nurse staffing. Methods: Data collected from 29,282 institutions between 1996 and 2013 were analyzed. Nurse staffing was measured as daily patient census per registered nurse (RN). The standard for general hospitals, hospitals, and clinics is 2.5 or less, and that for long-term care hospitals is 6.0 or less of the daily patient census per RN. Clinics may substitute nursing assistants for RNs by 50% or 100% depending on their daily inpatient census; long-term care hospitals may substitute nursing assistants for RNs by two thirds of the required number of RNs. Results: The proportion of general hospitals, hospitals, clinics, and long-term care hospitals meeting the standards was 63%, 19%, 63%, and 94%, respectively, in 2013. While general hospitals had an increase in the proportion during the 1996-2013 period, small changes were found in hospitals and clinics. In 2013, nurses were estimated to care for 16 (interquartile range: 12~24) patients per shift in general hospitals. Three quarters of clinics had no RNs in 2013. Conclusion: Many medical institutions did not meet the legally mandated minimum staffing level. The government must implement policy actions for all medical institutions to meet the legal standards.

• 간호행정학회지
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• 제19권5호
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• pp.565-577
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• 2013

Purpose: This study was done to propose an improvement in the Nursing Fee Differentiation Policy to alleviate polarization of nursing staffing level among hospitals and to rectify the confusion of legally mandated standards between the Korean Medical Law and National Health Insurance Act. Methods: The policy regulation was reconstructed related to nurse staffing standards and nurse-to-patients ratios. Data on nurse staffing grades were obtained from database of the Health Insurance Review & Assessment Service (HIRA) for the third quarter of 2010 for 44 tertiary hospitals, 274 general hospitals, and 1,262 hospitals. A break-even analysis was used to estimate financial burden of the revised policy improvement proposal. An industrial engineering method was used to calculate Nurse-to-Patients ratios per shift. Results: Twelve tertiary hospitals were downgraded. 74 general hospitals and 102 hospitals were upgraded after application of the regulation. Finances for total hospitalization expenditures changed from -3.55% to +3.14%. Conclusion: The results indicate that the proposed policy would decrease polarization between tertiary hospitals and small hospitals, and would not put a major strain on the finances of the Korean National Health Insurance. Therefore, it is suggested that government stake-holders and many interest groups consider this policy proposal and build a consensus.

• 대한화학회지
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• 제55권3호
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• pp.392-399
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• 2011

벤조산 유도체($NH_2$, OH, H, F, Cl, CN, NO, $NO_2$) 의 두 형태를 6-311++G(d,p) 바탕 집합을 이용하여 MP2, DFT 및 HF 수준으로 연구하였으며, cis이성 질체가 더 안정하였다. 벤조산의 수소 결합 형성은 안정화 에너지를 이용하여 추산하였으며, 이합체에 대한 수소결합 에너지 계산치는 고리에서 협동 상호작용이 일어남을 보여주었다. 페닐 고리로 전자를 밀어내는 그룹(ERG)은 더 안정한 수소 결합이 형성하였다. 이합체에서 O-H 결합의 적색이동은 -565.3에서 $-589.3\;cm^{-1}$ 범위였으며, 상호 작용의 특성은 NBO 분석을 이용하여 연구하였다.