• Title/Summary/Keyword: Lee Mi-Ja

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Proteomic Analysis of Cytokine-Like Proteins Secreted from Human Bronchial Epithelial Cells in Response to Pathogenic Bacterial Infection

  • Park, Mi-Ja;Oh, Mi-Jung;Jo, Dong-Hwan;Chin, Mi-Reyoung;Lee, Ji-Yeon;Park, Ji-Woo;Lee, Na-Gyong;Kim, Dae-Kyong
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.111.1-111.1
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    • 2003

  • Bacterial infection is a very complex process in which both pathogens and host cells play crucial roles, and the host cells undergo drastic changes in their physiology, releasing various proteins in response to the pathogenic infection. Human airway epithelial surface serves as a first line of defense against microorganisms and the external environment. It is well known that bronchial epithelial cells secrete various chemokines and cytokines such as IL-6 and IL-8 to cope with various respiratory pathogens. (omitted)

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Differential Induction of PepTLP Expression via Complex Regulatory System against Fungal Infection, Wound, and Jasmonic Acid Treatment during Pre-and Post-Ripening of Nonclimacteric Pepper Fruit

  • Jeon, Woong-Bae;Kim, Kwang-Sang;Lee, Hyun-Hwa;Cheong, Soo-Jin;Cho, Song-Mi;Kim, Sun-Min;Pyo, Byoung-Sik;Kim, Ynung-Soon;Oh, Boung-Jun
    • The Plant Pathology Journal
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    • v.20 no.4
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    • pp.258-263
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    • 2004

  • Ripe fruit of pepper (Capsicum annuum) showed resistance to Colletotrichum gloeoporioides, but unripe fruit was susceptible. We previously isolated the PepTLP gene that induced in both unripe and ripe fruit by fungal infection and wound, and only in ripe fruit by jasmonic acid (JA) treatment. To examine further regulation of PepTLP, the action of specific agonist and antagonists of known signaling effector on the .PepTLP expression by fungal infection, wound, and JA was investigated. A similar dephosphorylation event negatively activated all the PepTLP expression in the ripe fruit by fungal infection, wound, and JA. The induction of PepTLP expression by wound is differentially regulated via phosphorylation and dephosphorylation step during pre- and post-ripening, respectively. In addition, the induction of PepTLP expression in the ripe fruit by wound and JA is differentially regulated via dephosphorylation and phosphorylation step, respectively. Only both wound and JA treatment has synergistic effect on the PepTLP expression in the unripe fruit. Both SA and JA treatments on the unripe fruit, and both wound or JA and SA on the ripe fruit could not do any effect on the expression of PepTLP. These results suggest that the induction of PepTLP expression is differentially regulated via complex regulatory system against fungal infection, wound, and JA treatment during pre- and post-ripening of pepper fruit.

  • https://doi.org/10.5423/PPJ.2004.20.4.258 인용 PDF KSCI

Simultaneous Determination of Baicalin and Glycyrrhizin in Eul-Ja-Tang by HPLC/DAD

  • Lee, Mi-Kyeong;Lee, Ki-Yong;Kim, Seung-Hyun;Park, Jung-Hyun;Cho, Jung-Hee;Oh, Mi-Hyun;Baek, Ju-Hyun;Kim, Hyo-JIn;Kim, Young-Choong;Sung, Sang-Hyun
    • Natural Product Sciences
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    • v.14 no.3
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    • pp.147-151
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    • 2008

  • A high performance liquid chromatographic (HPLC) method for the simultaneous determination of marker constituents, baicalin and glycyrrhizin was established for the quality control of traditional herbal medicinal preparation, Eul-Ja-Tang (EJT). Separation and quantification were successfully achieved with a Waters XTerra RP18 column ($5{\mu}m$, 4.6 mm I.D. ${\times}$ 150 mm) by gradient elution of a mixture of acetonitrile and water containing 0.03% phosphoric acid (pH 2.03) at a flow rate of 1.0 mL/min. The diode-array UV/VIS detector (DAD) was used for the detection and the wavelength for quantification was set at 250 nm. The presence of baicalin and glycyrrhizin in this decoction was ascertained by retention time, spiking with each authentic standard and UV spectrum. Both baicalin and glycyrrhizin showed good linearity ($r^2$ > 0.999) in a relatively wide concentration ranges. The R.S.D. for intra-day and inter-day precision was less than 5% and the limits of detection (LOD) were about 30 ng. The mean recovery of each compound was 99.5 - 101.2% with R.S.D. values less than 4.0%. This method was successfully applied to the determination of contents of baicalin and glycyrrhizin in three commercial products of EJT, which resulted in the difference in the contents of these compounds. These results suggest that the developed HPLC method is simple, effective and could be readily utilized as a quality control method for commercial EJT products.

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