• Title/Summary/Keyword: Leaves extracts

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Antioxidative Activity of Extracts of Acanthopanax divaricatus var. albeofructus Leaves in Human Dermal Fibroblast Irradiated by UVA (자외선이 조사된 사람피부 섬유아세포에서 흰털오가피 잎추출물의 항산화작용)

  • Shin, Ai-Hyang;Lyu, Su-Yun;Noh, Bin-Na;Kim, Ja-In;Kim, Ok-Kyoung;Park, Won-Bong
    • YAKHAK HOEJI
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    • v.51 no.4
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    • pp.229-234
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    • 2007
  • We investigated antioxidative activity of the water and ethanol extracts of leaves of Acanthopanax divaricatus var. albeofructus in human dermal fibroblast (HDFs) irradiated by UVA. The irradiation of UVA did not affect the cell viability of HDFs. The antioxidative activity of the extract was investigated by xylenol orange, TBARS (thiobarbituric acid reactive substances) and antioxidant enzyme assay. Both extracts showed H202 scavenging activity and inhibited lipid peroxidation in HDF cells irradiated by UVA. The extracts also recovered enzyme activity in the same cells.

Phytotoxic Effects of Naturally Occurring Chemicals from Pinus koraiensis on Experimental Species (잣나무의 天然化學物質이 다른 植物에 미치는 毒性作用)

  • Kil, Bong-Seop;Kim, Doo-Young;Kim, Young-Sik;Lee, Seung-Yeob
    • The Korean Journal of Ecology
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    • v.14 no.2
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    • pp.149-157
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    • 1991
  • To find out allelopathic potential of naturally occurring substances emitted form pinus korairnsis, water extracts and volatile sudstances of the tree materials werw provided and used for tests such as seed germination and seedling growth test. In general, the more was the concentration of the extracts and essential oils, the worse was growth effects of the selected species. The germination an the growth were inversely proportional to the concentration of the extracts and the essential oils of p. koraiensis. in other words, the chemical substances had the biological toxic activity. gc and gc/ms methods were employed for analysis and identification of phytotoxic substances from the tree leaves. Sixteen chemical compounds were identified from water extracts and nineteen from essential oils of p. koraiensis leaves. Through the experiment on germination and seedling growth test, the chemical substances naturally occurring from p. koraiensis seemed to be responsible for the allelopathic potential in this study.

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The Effect of Willow Leaf Extracts on Human Leukemic Cells in Vitro

  • El-Shemy, Hany A.;Aboul-Enein, Ahmed M.;Aboul-Enein, Mostafa I.;Issa, Sohair I.;Fujita, Kounosuke
    • BMB Reports
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    • v.36 no.4
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    • pp.387-389
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    • 2003
  • The young developing leaves of willow (Salix safsaf, Salicaceae) trees have antileukemic activity. After a 24-h incubation in vitro, the crude water extracts of the leaves killed a majority of the blasts of acute myeloid leukemia (AML, 73.8%).

Comparison of Antioxidant, Cytotoxicity and Flavonoid Content of Stachys sieboldii Miq. vs. Lycopus lucidus Turcz. Leaf Extracts (초석잠 및 택란 잎 추출물의 항산화 및 세포독성 활성과 총 플라보노이드 함량 비교)

  • Na, Eun;Lee, Jung Woo;Lim, Sun Young
    • Journal of Life Science
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    • v.30 no.2
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    • pp.147-155
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    • 2020
  • In this study, the antioxidant and cytotoxic effects and the flavonoid contents of leaf extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz. were compared. The flavonoid contents of the acetone + methylene chloride (A+M) and methanol (MeOH) extracts of L. lucidus Turcz. leaves were 55.7 and 233.2 mg/g, respectively. In a DPPH assay, A+M and MeOH extracts from L. lucidus Turcz leaves had a greater scavenging effect than those of S. sieboldii Miq. leaves (p<0.05). In an ABTS assay, MeOH extracts from S. sieboldii Miq. and L. lucidus Turcz (0.5 mg/ml concentration) leaves had scavenging effects of 85% and 91%, respectively (p<0.05), suggesting that both of the MeOH extracts had greater scavenging effects than both A+M extracts. In a 120 min ROS production assay, all tested extracts decreased the cellular ROS production induced by H2O2 compared to that produced by exposure to the extract-free control. The MeOH extract from L. lucidus Turcz leaves had a greater inhibitory effect on cellular ROS production (p<0.05). Treatment with A+M and MeOH extracts from both S. sieboldii Miq. and L. lucidus Turcz. leaves showed a dose-dependent increased cytotoxicity against the growth of AGS, HT-29 cancer cells, and HT-1080 (p<0.05). Both A+M extracts had a greater inhibitory effect on the growth of all cancer cells than both MeOH extracts. These results suggest that the MeOH extract of L. lucidus Turcz. leaves is effective in scavenging free radicals and inhibiting cellular oxidation, while the A+M extract inhibits proliferation of three types of cancer cell.

Antimicrobial Effect of Mulberry Leaves Extracts Against Oral Microorganism (뽕잎 추출물의 구강미생물에 대한 항균효과)

  • Choi, Jeong-Lee;Jung, Mi-Ae;Jung, Sang-Hee
    • Journal of dental hygiene science
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    • v.6 no.4
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    • pp.251-254
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    • 2006
  • In the current research for natural product with antimicrobial effects, various extracts of Mulberry Leaves against microorganisms were evaluated in terms of the minimum inhibitory concentrations(MIC). In general, Candida albicans was more antimicrobial activity than the other microorganisms such as Streptococcus mutans, Staphylococcus epidermis, and Staphylococcus aureus. The maximum activity was exhibited by ethanol extract of the leaves of Mulberry Leaves against Candida albicans (MIC, $1600{\mu}g/ml$). These results suggest that ethanol and water extracts of Mulberry Leaves have a potential antimicrobial activity.

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Antioxidant and Biological Activity in the Leaves of Adzuki Bean (Vigna angularis L.)

  • Lee, Kyung Jun;Lee, Jung-Ro;Shin, Myoung-Jae;Cho, Gyu-Taek;Lee, Ho-Sun;Ma, Kyung-Ho;Lee, Gi-An;Chung, Jong-Wook
    • Korean Journal of Plant Resources
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    • v.31 no.3
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    • pp.237-253
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    • 2018
  • The adzuki bean (Vigna angularis L.) is a red-grained legume that has a number of essential nutrients and is used in traditional dishes in Asia. Adzuki bean industrial by-products are also a potential low-cost source of some unique bioactive polyphenols. Hence, here, the authors aimed to perform a comparative study of the phytochemical profiles of the leaves and seeds of the adzuki bean and compare their antioxidant, ${\alpha}$-glucosidase inhibition, and tyrosinase inhibition activity. The authors assessed antioxidant activity by DPPH, ABTS, FRAP, PR, TPC, and SOD assays, which showed wide variation, respectively. From the relative antioxidant capacity index results, 10 adzuki bean landraces were selected to compare for phytochemicals and bioactivity using leaf and seed extracts. Antioxidant, ${\alpha}$-glucosidase inhibition, and tyrosinase inhibition activity in the leaf extracts were higher than in the seed extracts, and there were more flavonols and isoflavones in the leaf extracts than in the seed extracts. This study demonstrated that adzuki bean leaf extracts could be a new natural antioxidant or antidiabetic agent and a skin whitener and can also be used in industrial applications.

In vitro eliminative effects three sorts of herbal extracts of against Ichthyophthirius multifiliis (3종의 식물 추출물에 의한 백점충의 in vitro 구제효과)

  • Kim, Jin-Do;Kim, Yi-Cheong;Jung, Sung-Hee;Seo, Jung-Soo;Park, Sung-Woo
    • Journal of fish pathology
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    • v.24 no.3
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    • pp.289-295
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    • 2011
  • The in-vitro eliminative effects of against three types of herbal extract and formalin Ichthyophthirius multifiliis were examined. All parasites were killed within one hour after exposure to the 500 fold dilution of the complex herb extract whereas the 10 fold dilution of the fertilized solution of Salvia plebeia R. Br. killed all parasites within one hour after exposure. The 5,000 fold dilution of the extracts of Ginkgo biloba leaves killed all parasites within one hour after exposure. As a comparative agent, formalin killed all parasites within one hour at 100 ppm. As the results, the extracts of Ginkgo biloba leaves extracts have the most eliminative ability against the parasite. No differences were found among different parasite density in eliminative effects of the three types of herbal extracts and formalin. Also there were no changes in the fish gill tissues after exposure for two hours to the 5,000 fold dilution of the extract of Ginkgo biloba leaves.

The Allelopathic Effects of Aqueous Chemicals of Ambrosia artemisiifolia on Selected Plants (돼지풀의 수용추출물이 수종 식물에 미치는 알레로파시 효과)

  • Kim, Hae-Su;Kim, Jong-Hui
    • The Korean Journal of Ecology
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    • v.24 no.1
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    • pp.67-73
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    • 2001
  • The allelochemicals from aqueous extracts of A. artemisiifolia var. elatior leaves and roots were analyzed and 60 compounds in the leaf and 53 compounds in the roots were inspected. The main compounds were acids,. especially phenolic acids and some non-acids. The total amount of compounds in the aqueous extracts of A. artemisiifolia var. elatior leaves was higher than in the roots. The aqueous extracts had much high inhibiting effects on the germination and seedling elongation of selected plants. In both cases, the inhibiting effects were very different with different selected plants and increased significantly as the concentration of aqueous extracts increased. Capsicum annum, Achyranthes japonica, and Oenothera odorta plants were suffered more significant inhibition effects than Raphnus sativus, Cucumis sativus, Brassica camperstris plants. Aqueous extracts from leaves had slightly higher inhibition effects than the aqueous extracts from the roots.

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Biofunctional Activities of Sanguisorbae officinalis L. Leaves Ethanol Extract (오이풀잎 에탄올 추출물에 대한 기능성 연구)

  • Park, Sung Jin;Rha, Young Ah
    • Culinary science and hospitality research
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    • v.21 no.5
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    • pp.171-179
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    • 2015
  • This study was conducted to investigate the antioxidant activity and the bioactive compounds found in 70% ethanol extracts taken from Sanguisorbae officinalis L. leaves(SO) cultivated in Korea. The extracts were tested for their total phenolic contents (TPC), total flavonoid contents (TFC), phenolic compounds, and antioxidative activities using various in vitro assay such as DPPH, ABTS radical scavenging activity, FRAP activity, and reducing power. The TPC and TFC were found to be $119.3{\pm}1.54mg$ gallic acid and $59.6{\pm}1.43mg$ rutin at mg of 70% ethanol extracts, respectively. Catechin was the major material among the phenolic compounds in SO extracts. The DPPH radical scavenging activity, ABTS radical scavenging activity, FRAP activity, and reducing power of SO extracts were increased in a dose-dependent manner. These results suggest that SO extracts could be considered as a good source of natural antioxidants and functional food ingredient.