• Journal of the Korean Society of Costume
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• v.52 no.8
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• pp.87-98
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• 2002

Recently, we consist in the crucial juncture have to develope culture goods which is worth standing for our nation's endemism. In this vein, this study tried to reproduce and apply it to the present time by the consideration of various elements such as the kind, the use and the fabrication of sewing craft-works was beloved in women's quarter called Kyubang in the Chosun dynasty. With this, this study was designed to develope craft-works which can make an appeal to foreigners with the merits between contemporary factor and traditional factor. The findings of this study were as follows; First, since economic system in the Chosun dynasty was based on home handicrafts and self-sufficiency, it was a commonplace of the Chosun dynasty that home industry such as sewing, spinning and sericulture was thriving among all the women without social ladder. And also, since women of the day were governed by confucian tradition and moral obligation, they mainly have to live in women's quarter called Kyubang restricted to visitors. For the reasons, Wives of the time have no choice but to spend their time by sewing in the promotion of virtues for woman. In additions, they made an effort to pray for the longevity, the wealthy and the fertility of their family members. Second, the sewing instruments is necessary for women's sewing. And also, since sewing instruments was considerably scientific, efficient in those shape and structure, this instruments could save wives a lot of trouble. Third, since many people were convinced of the functions as practicality as well as fanciness, the sewing craft-works were very useful to them in leading their daily life. Fourth, the reappeared works were a total of 22, and the applied works were a total of 17.

• Biomolecules & Therapeutics
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• v.23 no.6
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• pp.557-563
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• 2015

Skin aging is the most readily observable process involved in human aging. Ultraviolet B (UVB) radiation causes photo-oxidation via generation of reactive oxygen species (ROS), thereby damaging the nucleus and cytoplasm of skin cells and ultimately leading to cell death. Recent studies have shown that high levels of solar UVB irradiation induce the synthesis of matrix metalloproteinases (MMPs) in skin fibroblasts, causing photo-aging and tumor progression. The MMP family is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes such as arthritis and metastasis. We investigated the effect of diphlorethohydroxycarmalol (DPHC) against damage induced by UVB radiation in human skin keratinocytes. In UVB-irradiated cells, DPHC significantly reduced expression of MMP mRNA and protein, as well as activation of MMPs. Furthermore, DPHC reduced phosphorylation of ERK and JNK, which act upstream of c-Fos and c-Jun, respectively; consequently, DPHC inhibited the expression of c-Fos and c-Jun, which are key components of activator protein-1 (AP-1, up-regulator of MMPs). Additionally, DPHC abolished the DNA-binding activity of AP-1, and thereby prevented AP-1-mediated transcriptional activation. These data demonstrate that by inactivating ERK and JNK, DPHC inhibits induction of MMPs triggered by UVB radiation.

• Korean Journal of Head & Neck Oncology
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• v.16 no.2
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• pp.191-195
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• 2000

Objectives: Despite various attempts to preserve the normal voice in advanced laryngeal cancer, it is inevitable for many advanced laryngeal cancer patients to undergo total laryngectomy and thus making a trade off between quality and quantity of life. Laryngectomees are faced with voice loss, change in physical appearance and health deterioration which hinder their efforts to rehabilitate back into their family and employment leading to poor quality of life. The objectives of this study were to evaluate the quality of life in long term surviving laryngectomees to, define the factors which are most important in determining their present quality of life and to propose a theoretical model for quality of life after laryngectomy in Korea. Materials and Methods: From 1986 to 1995, 120 laryngectomees with no evidence of disease were followed up for at least 3years were evaluated. Each of 15 quality of life domains with a total of ten points were given for a quality of life score. Results: There was no significant difference in the physical-mental adaptation index such as general health and mental health between the laryngectomees and control group(p>0.05). However, social adaptation index such as social activity, occupational status and economic status for laryngectomees were significantly lower with 2.3, 3.5, and 4.4 points compared to control group with 8.5, 7.6, and 7.1 points respectively(p<0.05). Conclusion : Vocational and social rehabilitation should be emphasized with highest priority for improving their economic status and thus improving their quality of life.

• Structural Engineering and Mechanics
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• v.39 no.3
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• pp.427-447
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• 2011

The progressive collapse phenomenon is generally regarded as dynamic. Due to the impracticality of nonlinear dynamic computations for practitioners, an interest arises for the development of equivalent static pushover procedures. The present paper proposes a methodology to identify such a procedure for sudden column removals, using energetic evaluations to determine the pushover loads to apply. In a dynamic context, equality between the cumulated external and internal works indicates a vanishing kinetic energy. If such a state is reached, the structure is sometimes assumed able to withstand the column removal. Approximations of these works can be estimated using a static computation, leading to an estimate of the displacements at the zero kinetic energy configuration. In comparison with other available procedures based on such criteria, the present contribution identifies loading patterns to associate with the zero-kinetic energy criterion to avoid a single-degree-of-freedom idealisation. A parametric study over a family of regular steel structures of varying sizes uses non-linear dynamic computations to assess the proposed pushover loading pattern for the cases of central and lateral ground floor column failure. The identified quasi-static loading schemes are shown to allow detecting nearly all dynamically detected plastic hinges, so that the various beams are provided with sufficient resistance during the design process. A proper accuracy is obtained for the plastic rotations of the most plastified hinges almost independently of the design parameters (loads, geometry, robustness), indicating that the methodology could be extended to provide estimates of the required ductility for the beams, columns, and beam-column connections.

• The Journal of the Korea Contents Association
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• v.14 no.7
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• pp.70-82
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• 2014

The competitive circumstance among domestic TV stations caused by TV channels of comprehensive programming appearance in 2011 brought the rapid growth of talk shows. The purpose of this article is to diagnose the talk shows trend from a self-disclosure perspective to consider a social aspect and communication structure in popular TV programs. 26 domestic TV talk shows from 2011 to 2014 were collected and analyzed to understand current state and grasp genres and formats. The results show that self-disclosure talk shows are increasing under the leading of terrestrial TV channels. The talk only type of talk show is superior in numbers compare to the variety type talk show includes such as game and quiz. The popular talk shows were produced inside studio mainly with fun seeking topics. Multi MCs and guests encountered many to many communication structure. The marked changes among self-disclosure talk shows to encourage participation of entertainers' family members and ordinary people are emerging and it is a noteworthy phenomenon.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.11
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• pp.4609-4614
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• 2015

Background: In recent decades, the prognosis for childhood leukemia has improved, especially for acute lymphoblastic leukemia (ALL). In Thailand, though, the survival rate for ALL is unimpressive. In 2006, standard national protocols for childhood leukemia treatment were implemented. We herein report the outcome of the ALL national protocols and explanations behind discrepancies in outcomes between institutions. Materials and Methods: Between March 2006 and February 2008, 486 children with ALL from 12 institutions were enrolled in the Thai national protocols. There were 3 different protocols based on specific criteria: one each for standard risk, high risk and Burkitt's ALL. We classified participating centers into 4 groups of institutions, namely: medical schools in Bangkok, provincial medical schools, hospitals in Bangkok and provincial hospitals. We also evaluated supportive care, laboratory facilities in participating centers, socioeconomics, and patient compliance. Overall and event-free survival were determined for each group using the Kaplan Meier method. Statistical differences were determined using the log-rank test. Previous outcomes of Thai childhood ALL treatment between 2003 and 2005 served as the historic control. Results: Five-year overall survival of ALL treated using the Thai national protocol was 67.2%; an improvement from the 63.7% of the 12-institute historical control (p-value=0.06). There were discrepancies in event-free survival of ALL between centers in Bangkok and up-country provinces (69.9% vs 51.2%, p-value <0.01). Socioeconomics and patient compliance were key elements in determining the outcome (65.5% vs 47.5%, 59.4% vs 42.9%) (p-value < 0.02). Conclusions: Implementation of standard national protocols for childhood leukemia in Thailand did not significantly improve the outcome of ALL. Factors leading to better outcomes included (a) improvement of treatment compliance (b) prevention of treatment abandonment and (c) financial support to the family.

• Journal of Periodontal and Implant Science
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• v.39 no.sup2
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• pp.231-237
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• 2009

Purpose: Cytolethal distending toxin (CDT) is a family of heat-labile cytotoxins produced by several gram-negative mucosa-associated pathogens, including Aggregatibacter actinomycetemcomitans. CDT is well known to be capable of inducing growth arrest, morphological alterations, and eventually death in various cells. CDT belongs to a tripartite $AB_2$ toxin (CdtB: the enzymatic A subunit; CdtA and CdtC: the heterodimeric B subunit). Previous studies proposed that CdtA and CdtC together bind to a cell surface receptor and glycolipids act as a receptor for A. actinomycetemcomitans CDT (AaCDT). In this study, recombinant CdtA and CdtC proteins of AaCDT were co-expressed in a bacterial expression system and tested for their affinity for $GM_1$ ganglioside. Methods: The genes for CdtA and CdtC from A. actinomycetemcomitans Y4 were utilized to construct the expression vectors, pRSET-cdtA and pET28a-cdtC. Both CdtA and CdtC proteins were expressed in Escherichia coli BL21(DE3) and then purified using hexahistidine (His6) tag. The identity of purified protein was confirmed by anti-His6 antibody and monoclonal anti-CdtA antibody. Furthermore, the affinity of recombinant protein to $GM_1$ ganglioside was checked through ELISA. Results: Recombinant CdtA and CdtC proteins were expressed as soluble proteins and reacted to anti-His6 and monoclonal anti-CdtA antibodies. ELISA revealed that purified soluble CdtA-CdtC protein bound to $GM_1$ ganglioside, while CdtA alone did not. Conclusions: Co-expression of CdtA and CdtC proteins enhanced the solubility of the proteins in E. coli, leading to convenient preparation of active CdtA-CdtC, a critical material for the study of AaCDT pathogenesis.

• Journal of Korean Home Economics Education Association
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• v.16 no.3
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• pp.147-161
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• 2004

This study investigated laundry behavior. laundry awareness. clothing behavior for environment preservation of university students living at dormitory and relationship among the students' backgrounds. above three aspects. The survey data were collected from the students at Korea National University of Education located in Chung-Buk on November, 2003. The results showed that the laundry awareness of university students living at dormitory was relatively high, but the students' actual laundry behavior was unscientific and according to customs leading to waste of various resources and water pollution. The students' clothing behavior for environment preservation occurred very rarely. Female students and the students had laundry education were superior to laundry awareness and laundry behavior. Therefore, it will be necessary to develop and enforce laundry education program which is not just providing knowledge of laundry but improving power of practical application to real life situation for university students who might be one of family members in immediate future.

• Molecules and Cells
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• v.37 no.7
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• pp.526-531
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• 2014

Human PARP family consists of 17 members of which PARP-1 is a prominent member and plays a key role in DNA repair pathways. It has an N-terminal DNA-binding domain (DBD) encompassing the nuclear localisation signal (NLS), central automodification domain and C-terminal catalytic domain. PARP-1 accounts for majority of poly-(ADP-ribose) polymer synthesis that upon binding to numerous proteins including PARP itself modulates their activity. Reduced PARP-1 activity in ageing human samples and its deficiency leading to telomere shortening has been reported. Hence for cell survival, maintenance of genomic integrity and longevity presence of intact PARP-1 in the nucleus is paramount. Although localisation of full-length and truncated PARP-1 in PARP-1 proficient cells is well documented, subcellular distribution of PARP-1 fragments in the absence of endogenous PARP-1 is not known. Here we report the differential localisation of PARP-1 Nterminal fragment encompassing NLS in PARP-$1^{+/+}$ and PARP-$1^{-/-}$ mouse embryo fibroblasts by live imaging of cells transiently expressing EGFP tagged fragment. In PARP-$1^{+/+}$ cells the fragment localises to the nuclei presenting a granular pattern. Furthermore, it is densely packaged in the midsections of the nucleus. In contrast, the fragment localises exclusively to the cytoplasm in PARP-$1^{-/-}$ cells. Flourescence intensity analysis further confirmed this observation indicating that the N-terminal fragment requires endogenous PARP-1 for its nuclear transport. Our study illustrates the trafficking role of PARP-1 independently of its enzymatic activity and highlights the possibility that full-length PARP-1 may play a key role in the nuclear transport of its siblings and other molecules.

• Molecular & Cellular Toxicology
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• v.1 no.2
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• pp.73-77
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• 2005

Nickel is the one of potent environmental, the occupational pollutants and the classified human carcinogens. It is a serious hazard to human health, when the metal exposure. To prevent human diseases from the heavy metals, it is seemingly important that understanding of how nickel exerts their toxicity and carcinogenic effect at a molecular and a genomic level. The process of nickel absorption has been demonstrated as phagocytosis, iron channel and diffusion. Uptaked nickel has been suggested to induce carcinogenesis via two pathways, a direct DNA damaging pathway and an indirect DNA damaging pathway. The former was originated from the ability of metal to generate Reactive Oxygen Species (ROS) and the reactive intermediates to interact with DNA directly. Ni-generated ROS or Nickel itself, interacts with DNAs and histones to cause DNA damage and chromosomal abnormality. The latter was originated from an indirect DNA damage via inhibition of DNA repair, or condensation and methylation of DNA. Cells have ability to protect from the genotoxic stresses by changing gene expression. Microarray analysis of the cells treated with nickel or nickel compounds, show the specific altered gene expression profile. For example, HIF-I (Hypoxia-Inducible Factor I) and p53 were well known as transcription factors, which are upregulated in response to stress and activated by both soluble and insoluble nickel compounds. The induction of these important transcription factors exert potent selective pressure and leading to cell transformation. Genes of metallothionein and family of heat shock proteins which have been known to play role in protection and damage control, were also induced by nickel treatment. These gene expressions may give us a clue to understand of the carcinogenesis mechanism of nickel. Further discussions on molecular and genomic, are need in order to understand the specific mechanism of nickel toxicity and carcinogenicity.