• 제목/요약/키워드: Larvae density

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Community structure of sessile organisms on PVC plates according to different submerged timings and durations in Jangmok Bay, Korea (남해 장목만에서 PVC판 투입시기와 투입기간에 따른 부착생물군집 구조)

  • Park, So-Hyun;Seo, Jin-Young;Choi, Jin-Woo
    • The Korean Journal of Malacology
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    • 제27권2호
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    • pp.99-105
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    • 2011
  • This study was conducted to compare the species composition of sessile organisms on the artificial substrates of PVC submerged at different time intervals and duration in Jangmok Bay, Geoje Island, southern coast of Korea. Three PVC plates were submerged at one month interval from March to October and retrieved in November, 2007. A mussel, Mytilus galloprovincialis exclusively occupied the artificial substrates submerged from March to April and occurred as a dominant species to July. An ascidian, Styela plicata occurred as a dominant sessile species from May to August. Balanus amphtrite, Bugula sp., and hydrozoans occurred as dominant species on the plates submerged from July to September. There was a mis-match between the peak time of settlement and dominance of sessile organisms due to the interspecies competitions when the PVC plates were retrieved in November. There was no clear relationship between submerged duration and the abundance of sessile organisms due to the different settlement period. M. galloprovincialis seemed to be a strong competitor which could exclude the previous recruiters of macroalgae by overgrowth and occupy the substrate surface and maintain its high population density by preventing the settlement of other species until late autumn. These results suggested that the composition of sessile organisms in vacant hard substrates could be determined by the combined effects of supply-timing of larvae and post-settlement competitions.

The Distribution Pattern of Eggs and Larval Survival Rate of Phyllocnistis citrella (Lepidoptera: Gracillaridae) on the Leaves of Shoots in a Citrus Orchard in Jeju (제주 감귤원에서 귤굴나방 알의 신초 잎 위치별 분포와 유충의 생존률)

  • Hyun, Seung Young;Kim, Su Bin;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • 제58권3호
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    • pp.175-182
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    • 2019
  • The citrus leafminer, Phyllocnistis citrella (Lepidoptera: Gracillaridae), is an important pest that decreases the productivity of citrus trees by attacking citrus leaves, and especially it causes a severe damage during the young growth stage. In this study, we investigated the distribution of eggs on the leaves of shoots according to the position and the survival rate of this pest in the field condition. The occurrence patterns of P. citrella eggs in citrus orchards were largely affected by the occurrence of shoots. Even if the adults were still being attracted in the pheromone traps, the eggs were not observed on leaves when the growth of shoots was stopped. The density of P. citrella eggs in the citrus shoots peaked at the fifth or sixth leaf from the terminal leaf, and over 90% of the eggs were found in the first to eighth leaves as a whole. In 2015 and 2016, the average survival rate of larvae surviving to pupa was 1.4%, and the rate of survival to adults was 0.2%, indicating that most of them died. Furthermore, we have discussed the management strategy of P. citrella considering the distribution of eggs and the larval survival rate in the citrus shoots.

Co-occurrence of Matsumuraeses falcana and M. phaseoli (Lepidoptera: Tortricidae) in Soybean Fields, and Polymorphism of Cytochrome c Oxidase Subunit 1 Gene Nucleotide (콩 포장에서 어리팥나방과 팥나방(나비목: 잎말이나방과)의 동시 발생과 시토크롬 c 산화효소 1 유전자 염기서열의 다형성)

  • Jin Kyo, Jung;Eun Young, Kim;Taeman, Han
    • Korean journal of applied entomology
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    • 제61권4호
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    • pp.641-649
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    • 2022
  • Leaf-rolling moths were collected from soybean fields and identified as Matsumuraeses falcana and Matsumuraeses phaseoli by comparison with laboratory-reared species based on the nucleotide sequence (658 bp) of the mitochondrial cytochrome c oxidase 1 subunit gene (COX1). Ten haplotypes with 0.15-0.46% genetic distance from each other in COX1 were found in 47 samples of M. falcana, in which haplotype A was dominant (approximately 70%). Only one type of COX1 was found in 30 samples of M. phaseoli, and its sequence showed 4.11-4.61% genetic distance from those of M. falcana. Amino acid sequences translated from COX1 were identical in all samples of both species, and they showed synonymous substitutions. Larvae of both species caused damage to soybean leaves and pods and co-occurred simultaneously in the field. The average density of M. falcana was 1.5 times higher than that of M. phaseoli. The results clearly indicate that soybean was the host plant for both species. In addition, Elodia flavipalpis (Diptera: Tachinidae) was found to be a larval parasitoid of Matsumuraeses sp. through identification of the COX1 gene.

Damage and biological control of dark winged fungus gnats, Lycoriella ingenua (Diptera: Sciaridae) in a shiitake cultivation (표고 톱밥재배에서 검정날개버섯파리 Lycoriella ingenua (Diptera: Sciaridae)의 피해와 생물적 방제)

  • Kim, Hyeong-Hwan;Cho, Myoung-Rae;Kang, Taek-Jun;Ahn, Seung-Joon;Jeon, Sung-Wook;Lee, Chan-Jung;Cheong, Jong-Chun
    • Journal of Mushroom
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    • 제10권4호
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    • pp.184-190
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    • 2012
  • Monitorings and management experiment of adult Lycoriella ingenua (Diptera: Sciaridae) in artificial sawdust grown shiitake mushroom cultivation were carried out by using yellow sticky traps and Hypoaspis aculeifer (Acari: Laelapidae) at Hwaseong, Buyeo and Cheongwon in 2012. The larvae of L. ingenua reduced commercial value of the mushroom by direct eating or retarding the growth of mushroom by spongifying the sawdust medium. The number of L. ingenua caught by traps showed the highest peak in late september resulting in 127.5~1,025.7, 87.4~743.6 and 133.7~650.4 individuals per trap in Hwaseong, Buyeo and Cheongwon, respectively. Damage rate of fruiting bodies in shiitake mushroom by L. ingenua were 7.7~30.3% in Hwaseong, 6.7~25.3% in Buyeo and 5.3~26.0% in Cheongwon and showed the highest peak in late september. L. ingenua were effectively controlled when 30.3 individuals of H. aculeifer per $m^2$ inoculated for three time with 7 to 14 days of interval. In Hwaseong, Buyeo and Cheongwon, density of L. ingenua which were caught on the yellow sticky traps were 168.2, 126.1, 132.5 individuals, respectively. And, damage rate of fruiting bodies by L. ingenua were 5.9%, 5.5% and 5.4% respectively. Both of the L. ingenua density and damage rate of mushroom reduced more than 60% in all experimental site in contrast to the control treatment.

Utilization of meiobenthos for pollution monitoring in the Gamak Bay, Korea (가막만의 중형저서생물을 활용한 오염모니터링)

  • KIM Dong-Sung;JE Jong-Geel;SHIN Sang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • 제33권4호
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    • pp.307-319
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    • 2000
  • Community structure (seasonal fluctuation), taxon diversity, nematodes/benthic harpacticoids and nematodes/kinorhynchs ratios of meiobenthos were studied in the Gamak Bay, Korea. A series of samplings were carried out from April 1997 to May 1999. Meiobenthic organisms was collected by van Veen grab and three subcores of 34 mm in internal diameter were taken from each sediment sample. The total density of meiobenthos in each seasons was found to be the highest in May 1999, and the lowest in December 1998. Total density of meiobenthos at each station was the highest at Station 9 in May 1999 ($2,218 inds./10 cm^2$) and the lowest at Station 3 in December 1998 ($2 inds./10 cm^2$). The Gamak Bay seemed to have an individual number increased from the inner toward the outer stations. The highest number of meiobenthos was observed on 0.125 and 0.063 mm mesh size. In every season, over $80{\%}$ of the meiobenthos was composed of only four groups: nematodes, benthic harpacticoids, sarcomastigophorans and nauplius larvae of crustaceans. Seasonal fluctuation of meiobenthos was also studied. The nematodes/benthic harpacticoids(nematodes/kinorhynchs) ratios were studied as an index of pollution monitoring for benthic ecosystem at each station in each season. The value of nematodes/benthic harpacticoids ratio was the highest at the inner stations and the lowest at the outer stations in the Gamak Bay. Taxon diversity (H') was the highest at the outer stations and the lowest at the inner stations in the Gamak Bay. The value of nematodes/benthic harpacticoids ratio significantly increased from the outer toward the inner stations, whereas the taxon diversity (H') significantly increased from the inner toward the outer stations in Gamak Bay.

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Intensive Culture of the Pacific White Shrimp Litopenaeus vannamei under Limited Water Exchange I. Indoor Nursery Culture of Postlarvae (사육수 비교환 방식에 의한 흰다리새우의 고밀도 사육 I. 후기유생(postlarva)의 실내 중간육성)

  • Jang, In-Kwon;Kim, Jong-Sheek;Cho, Kook-Jin;Seo, Hyung-Chul;Cho, Yeong-Rok;Gopalakannan, Ayyaru;Kim, Bong-Lae
    • Journal of Aquaculture
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    • 제21권4호
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    • pp.339-345
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    • 2008
  • Farming of the fleshy shrimp Fenneropenaeus chinensis which is a major cultured species in the west coast of South Korea, has been suffered :trom mass mortality due to disease epizootics including viruses. Since the Pacific white shrimp Litopenaeus vannamei was introduced to Korea in 2003, farming of this species has rapidly increased for years, occupying 62.5% of total cultured shrimp production in 2007. However the studies on L. vannamei culture methods for shrimp farming situations in Korea are very limited. Nursery culture of shrimp larvae has some advantages including increased survival, improved feed efficiencies, enhanced growth performance and reduced grow-out period. In this study, L. vannamei postlarvae (${PL_3}-{PL_{10}}$) with a density of $3,750-9,090/m^3$ were cultured in four raceways under limited water exchange condition for 35 days. Survival was the highest (93.6%) in tank stocked with $4,090/m^3$ and was the lowest in tank with $9,090/m^3$ (58.1 %). Mean body weight at harvest ranged from 0.071 to 0.108 g, and FCR was 0.59-0.70 in all tanks. Concentration of total ammonia nitrogen was increased up to 20 ppm on day 10 in all tanks and thereafter gradually decreased by the third week of culture. Nitrite-nitrogen was rapidly increased from the third week, representing bio-floc condition by developed nitrifying bacterial community. Of the present nursery system some modification of structure and consideration for commercial scale are needed in order to be implemented to shrimp farmers.

Study on Soluble Concentrate Formulation and Quality Control Techniques of a Microbial Insecticide "Bt-Plus" (미생물살충제 "비티플러스" 액상 제형화 및 품질 분석 기술에 관한 연구)

  • Eom, Seonghyeon;Park, Hyeonji;Kim, Kyusoon;Hong, Youkyeong;Park, Jiyeong;Choi, Bongki;Kim, Joonsung;Kim, Kunwoo;Kang, Moonsoo;Yang, Kyunghyung;Kim, Yonggyun
    • Korean journal of applied entomology
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    • 제52권2호
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    • pp.115-123
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    • 2013
  • A microbial insecticide "Bt-Plus" has been developed to enhance an insecticidal efficacy of an entomopathogenic bacterium, Bacillus thuringiensis (Bt). However, its wettable powder formulation is not preferred by farmers and industry producers due to relatively high cost. This study aimed to develop a soluble concentrate formulation of Bt-Plus. To this end, an optimal mixture ratio of two bacterial culture broths was determined to be 5:4 (v/v) of Bt and Xenorhabdus nematophila (Xn) along with 10% ethanol preservative. In addition, Bt broth was concentrated by 10 times to apply the mixture at 1,000 times fold dilution. The resulting liquid formulation was sprayed on cabbage crop field infested by late instar larvae of the diamondback moth, Plutella xylostella. The field assay showed about 77% control efficacy at 7 days after treatment, which was comparable to those of current commercial biopesticides targeting P. xylostella. For storage test in both low and room temperatures, the liquid formation showed a relatively stable control efficacy at least for a month. To develop a quality control technique to exhibit a stable control efficacy of Bt-Plus, Bt spore density ($5{\times}10^{11}$ spores/mL) and eight active component concentrations of Xn bacterial metabolites in the formulation products have been proposed in this study.

Collection of Adult and Larval Mosquitoes in U.S. Army Compounds in the Republic of Korea During 1979~1983 (한국에 주둔한 미군지역내에서의 모기 성충과 유충 채집(1979~1983))

  • ;R.K
    • Parasites, Hosts and Diseases
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    • 제22권1호
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    • pp.102-108
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    • 1984
  • This paper is the result of adult female mosquito and larval collection in U.S. Army Installations in Korea from 1979 to 1983. New Jersey light traps were operated for adult collection from May to October. The primary concern of this surveillance is to determine when to recommend insecticide spraying for mosquito control in the Army areas. The 5th Preventive Medicine Unit have developed an "index" level of female mosquitoes in a light trap similar to other U.S. Army Agencies in other parts of the world. When 10 female mosquitoes are reached on two consecutive trap-night, or 5 known vector females are collected, fogging is recommended in the trap areas. 1. Mosquito collections were conducted in 12 U.S. Army areas by operating 39 New Jersey light traps. Mosquitoes collected from the areas were identified to be 17 species comprising 3 genera. Anopheles sinensis (40%), Culex tritaeniorhynchus (31%), Aedes vexans nipponii (19 %) and Culex pipiens pallens (10%) appeared to be the most common species in the areas. 2. The species, population density and monthly appearance of adult mosquitoes were found to be almost the same in the all provinces involved. And Japanese Encephalitis vector mosquitoes, Culex tritaeniorhynchus, showed their seasonal fluctuation from July to September with a peak in August each year. 3. Larval habitats confirmed in the Army areas were categorized into 16 types as shown in Table 3. The mosquito larvae collected in those habitats were identified to be 15 species representing 4 genera. Most breeding sites in the Army areas were those which are activated during the wet season. 4. More mosquitoes were collected from the Kyungki Province than from the other Provinces. The reason for more collection of mosquitoes from military installations in the Kyungki Province appears to be the geographic characteristics surrounded by rice fields, marshes and other stagnant water areas.

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Effect of rice seed dressing with imidacloprid WS on early occurring rice insect pests (본답 초기해충의 생력방제를 위한 imidacloprid의 종자분의 효과)

  • Choi, Byung-Ryul;Yoo, Jae-Ki;Lee, Sang-Guei;Lee, Jeong-Oon
    • The Korean Journal of Pesticide Science
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    • 제2권1호
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    • pp.85-90
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    • 1998
  • A series of experiments were carried out to determine the effect of seed dressing application of chemicals on the early occurring rice insect pests. The number of small brown planthopper adults per 3hills in seed-dressing plot of Imidacloprid WS (Im WS) was 1.6 and its control efficacy was over 90%, compared with that of untreated plot. Rice water weevil was observed in the density of 8.8 larvae per 5 hills seed-dressed Im WS plots(showing 95.5% of control efficacy). Control efficacy of Im WS and Im GR against adult weevils lasted for 26 days after treatment and was higher than that of Carbofuran GR. Rates of injured stems by rice stem maggot and injured leaves by rice leaf miner were 3% and 3.7% in Im WS treatment plot respectively. Seed germination rate after seed dressing with recommended dosage (3 g/seed kg) of Im WS was 71 % on the 1st day and increased on 5th day up to that of untreated seed. The residual amount of Im in seed dressing plot was 0.11 ppm in rice roots and 0.05 ppm in leaves on the 40th day after treatment. Residual effect of Im WSI sustained for 50 days with over 95% insecticidal effect for the rice water weevil and over 90% for the brown planthopper. Quantity of the chemical applied in the field was calculated as 0.084 kg a.i./ha in seed dressing and 0.3 kg a.i./ha in seed box treatment, respectively It took 1 hour to treat insecticide by seed dressing, 2.5 hours by seed box applying, and 3.6 hours by water surface releasing per 990 $m^2$.

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