Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Study on Soluble Concentrate Formulation and Quality Control Techniques of a Microbial Insecticide "Bt-Plus"

미생물살충제 "비티플러스" 액상 제형화 및 품질 분석 기술에 관한 연구

  • Eom, Seonghyeon (Department of Bioresource Sciences, Andong National University) ;
  • Park, Hyeonji (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Kyusoon (Department of Bioresource Sciences, Andong National University) ;
  • Hong, Youkyeong (Department of Bioresource Sciences, Andong National University) ;
  • Park, Jiyeong (Department of Bioresource Sciences, Andong National University) ;
  • Choi, Bongki (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Joonsung (Department of Bioresource Sciences, Andong National University) ;
  • Kim, Kunwoo (Department of Bioresource Sciences, Andong National University) ;
  • Kang, Moonsoo (YA Korea, Inc.) ;
  • Yang, Kyunghyung (YA Korea, Inc.) ;
  • Kim, Yonggyun (Department of Bioresource Sciences, Andong National University)
  • 엄성현 (안동대학교 자연과학대학 생명자원과학과) ;
  • 박현지 (안동대학교 자연과학대학 생명자원과학과) ;
  • 김규순 (안동대학교 자연과학대학 생명자원과학과) ;
  • 홍유경 (안동대학교 자연과학대학 생명자원과학과) ;
  • 박지영 (안동대학교 자연과학대학 생명자원과학과) ;
  • 최봉기 (안동대학교 자연과학대학 생명자원과학과) ;
  • 김준성 (안동대학교 자연과학대학 생명자원과학과) ;
  • 김건우 (안동대학교 자연과학대학 생명자원과학과) ;
  • 강문수 ((주) YA 코리아) ;
  • 양경형 ((주) YA 코리아) ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Received : 2012.12.26
  • Accepted : 2013.04.05
  • Published : 2013.06.01
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Abstract

A microbial insecticide "Bt-Plus" has been developed to enhance an insecticidal efficacy of an entomopathogenic bacterium, Bacillus thuringiensis (Bt). However, its wettable powder formulation is not preferred by farmers and industry producers due to relatively high cost. This study aimed to develop a soluble concentrate formulation of Bt-Plus. To this end, an optimal mixture ratio of two bacterial culture broths was determined to be 5:4 (v/v) of Bt and Xenorhabdus nematophila (Xn) along with 10% ethanol preservative. In addition, Bt broth was concentrated by 10 times to apply the mixture at 1,000 times fold dilution. The resulting liquid formulation was sprayed on cabbage crop field infested by late instar larvae of the diamondback moth, Plutella xylostella. The field assay showed about 77% control efficacy at 7 days after treatment, which was comparable to those of current commercial biopesticides targeting P. xylostella. For storage test in both low and room temperatures, the liquid formation showed a relatively stable control efficacy at least for a month. To develop a quality control technique to exhibit a stable control efficacy of Bt-Plus, Bt spore density ($5{\times}10^{11}$ spores/mL) and eight active component concentrations of Xn bacterial metabolites in the formulation products have been proposed in this study.

곤충병원세균(Bacillus thuringiensis: 비티)의 살충효과를 증가시킨 미생물살충제 "비티플러스"가 개발되었다. 그러나 수화제 형태의 비티플러스는 농가나 산업체에서 높은 단가로 선호하지 않고 있는 실정이다. 이에 본 연구는 액상 제형의 비티플러스를 개발하는 연구 목적을 두었다. 이러한 목적에 따라 먼저 비티플러스 제조에 포함되는 두 세균 배양액의 최적 혼합 비율을 결정하였다. 이 최적 혼합액은 10%의 에탄올을 보존제로 사용되었으며, 비티와 또 다른 곤충병원세균인 Xenorhabdus nematophila (Xn)의 배양액 비율이 5:4 (v/v)로 제조하게 했다. 또한 이 액상제형이 1,000 희석배수에서 효과를 보이기 위해 비티 배양액을 10 배 농축하여 최적 비율로 혼합하였다. 이렇게 해서 얻어진 액상 제형을 발육후기 유충의 배추좀나방(Plutella xylostella)이 가해를 하는 배추밭에 처리하여 7일 후 약 77%의 방제 효과를 보였는데, 이 처리 효과는 현재 상용화되는 배추좀나방 적용 생물농약들과 비등한 효과를 나타내는 것으로 판명되었다. 저온 및 상온의 저장 분석에서 본 액상 제형은 최소한 한 달 동안 안정된 방제효과를 나타내는 것으로 분석되었다. 이 액상 제형의 안정된 방제 효과를 보장하여 주는 품질관리 기술을 개발하기 위해 제형 내 비티 포자 밀도와 Xn 유래 유용물질의 농도를 판별하였다. 본 연구 분석은 최적의 방제 효과를 나타내기 위해서는 비티플러스 액상 제형에 비티포자 밀도가 최소 $5{\times}10^{11}$ spores/mL 이고, Xn 세균 대사물질들 가운데 8 종 유효물질의 농도가 품질관리 판별 기준으로 제시되었다.

Keywords

References

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